Lionel Forestier

University of Limoges | UNILIM

BISCEm (https://www.unilim.fr/biscem/), acronyme de Biologie Intégrative Santé Chimie Environnement, est une plateforme technologique créée par l’Institut de recherche GEIST en 2017. La plateforme regroupe des équipements de pointe permettant la réalisation d’analyses moléculaires, cellulaires et tissulaires et d’expériences sur des animaux, dans l...

The mutation T3811 ➜ G3811 (TG3811) discovered in the myostatin gene of the Blonde d’Aquitaine breed is suspected of contributing to the outstanding muscularity of this breed. An experiment was designed to estimate the effect of this mutation in a F2 and back-cross Blonde d’Aquitaine x Holstein population. By genotyping all known mutations in the m...

The aim of this study was to identify relevant biomarkers for the prognosis of glioma considering current molecular changes such as IDH mutation and 1p19q deletion. Gene expression profiling was performed using the TaqMan Low Density Array and hierarchical clustering using 96 selected genes in 64 patients with newly diagnosed glioma. The expression...

The transcriptional co-activator PGC-1α has been reported to play a key role in adaptive thermogenesis and to influence muscle homeostasis and growth in mouse and human. PGC-1α has a complex structure with multiple protein domains whose gene is controlled by two promoters and is subject to alternative splicing events. In cattle, very little is curr...

alpha 1-Antichymotrypsin is encoded by the unique SERPINA3 gene in humans, while it is encoded by a cluster of eight closely related genes in cattle. BovSERPINA3 proteins present a high degree of similarity and significant divergences in the reactive centre loop (RCL) domains which are responsible for the antiprotease activity. In this study, we an...

BACKGROUND: Transcriptome sequencing is a powerful tool for measuring gene expression, but as well as some other technologies, various artifacts and biases affect the quantification. In order to correct some of them, several normalization approaches have emerged, differing both in the statistical strategy employed and in the type of corrected biase...

Myostatin is essential for proper regulation of myogenesis, and inactivation of Myostatin results in muscle hypertrophy. Here, we identified an unexpected mutation in the myostatin gene which is almost fixed in Blonde d'Aquitaine cattle. In skeletal muscle, the mutant allele was highly expressed leading to an abnormal transcript consisting of a 41-...

Protease-activated receptor-2 (PAR-2) is a trypsin-activated member of the PAR receptor family. Following cleavage, the newly created N-terminus acts as a tethered receptor-activating ligand. PAR-2 is expressed in keratinocytes and enhances the phagocytosis rate of keratinocytes, which leads to increased melanosome transfer and skin pigmentation. H...

Glioblastoma Multiforme (GBM) is the most frequent malignant brain tumor with still poor prognosis. Tumor initiation, growth and recurrences might depend on Brain Tumor Stem Cells (BTSCs) which can promote tumor aggressiveness and potentially affords new therapeutic target. Recent works emphasized aberrant cell-surface glyco-conjugate expression in...

New preservation solutions are emerging, of various ionic compositions and with hydroxyethyl starch replaced by polymers such as polyethylene glycols (PEGs), offering the potential for 'immunocamouflage'. This experimental study investigated which of three clinically available preservation protocols offered the best graft protection, based on epith...

GAPDH cDNA fragment amplified from retrotranscribed RNA extracted from skin specimens. Lines A and B: from black hair skin regions of a Rhinelander rabbit. Lines C and D: from red hair skin regions of a Rhinelander rabbit. Line E: control genomic DNA. Line F: DNA ladder.

Tricolour rabbits with Δ6J/Δ30 (A) and Δ6J/Δ6J (B) genotypes. Rabbits with these two genotypes differ in terms of extension of black regions.

Three F1 rabbit families obtained crossing animals of different breeds with different genotypes at the Extension locus. Family 1 is obtained crossing a Rhinelander buck with a Thuringian doe, Family 2 is obtained crossing a Rhinelander buck with a Japanese doe, Family 3 is obtained crossing a Checkered Giant buck with a Japanese doe. The c.280_285d...

List of cattle CNVRs reported in four other experiments [49-52]and overlapping with goat CNVRs. CNVRs identified in cattle have been merged from the four reported experiments [49-52]. Progressive CNVR number has been assigned using the complete list. CNVRs are indicated with nucleotide positions (begin and end) on the Btau_4.0 version. Information...

In the domestic rabbit (Oryctolagus cuniculus), classical genetic studies have identified five alleles at the Extension locus: ED (dominant black), ES (steel, weaker version of ED), E (wild type, normal extension of black), eJ(Japanese brindling, mosaic distribution of black and yellow) and e (non-extension of black, yellow/red with white belly). S...

The agouti locus encodes the agouti signalling protein (ASIP) which is involved in determining the switch from eumelanin to pheomelanin synthesis in melanocytes. In the domestic rabbit (Oryctolagus cuniculus) early studies indicated three alleles at this locus: A, light-bellied agouti (wild type); a(t), black and tan; a, black nonagouti. We charact...

A central event in the formation of infectious prions is the conformational change of a host-encoded glycoprotein, PrP(C), into a pathogenic isoform, PrP(Sc). The molecular requirements for efficient PrP conversion remain unknown. Altered glycosylation has been linked to various pathologies and the N-glycans harbored by two prion protein isoforms a...

The bovine PRKAG3 gene encodes the AMPK gamma3 subunit, one isoform of the regulatory gamma subunit of the AMP-activated protein kinase (AMPK). The AMPK plays a major role in the regulation of energy metabolism and mutations affecting the genes encoding the gamma subunits have been shown to influence AMPK activity. The gamma3 subunit is involved in...

Several lines of evidence indicate that some glycoconjugates are efficient effectors of the cellular prion protein (PrPC) conversion into its pathogenic (PrPSc) isoform. To assess how glycoconjugate glycan moieties participate in the biogenesis of PrPSc, an exhaustive comparative analysis of the expression of about 200 glycosylation-related genes w...

Several lines of evidence indicate that some glycoconjugates are efficient effectors of the cellular prion protein (PrPC) conversion into its pathogenic (PrPSc) isoform. To assess how glycoconjugate glycan moieties participate in the biogenesis of PrPSc, an exhaustive comparative analysis of the expression of about 200 glycosylation-related genes w...

Glycosylation of mucins produced by human intestinal goblet cells plays a crucial role in their functions: mucus gel physico-chemical protective properties, host-bacteria interactions, cell-cell adhesion, cell migration, and cell signaling. Colonic mucin glycosylation can be modified by luminal metabolites of fiber fermentation like butyrate. Our a...

The RNase protection assay (RPA) is an extremely sensitive procedure for detection of messenger RNA (mRNA) in complex sample mixture of total RNA. However, its usefulness has been limited by the requirement for the DNA to be cloned onto an appropriate vector. We have utilized the polymerase chain reaction (PCR) to directly incorporate a T7 RNA poly...

Classic Bartter syndrome and the related disorders designated antenatal Bartter syndrome and Gitelman syndrome are relatively uncommon primary tubular disorders that usually run in families, with transmission on an autosomal recessive basis. Shared features include renal potassium wasting, metabolic alkalosis, hyperreninemia responsible for hyperal...

Alport syndrome is an inherited disorder characterized by progressive hematuric nephritis with structural defects of the glomerular basement membrane, and sensorineural deafness. Ocular abnormalities are frequently associated whereas macrothrombocytopenia or diffuse esophageal leiomyomatosis have been reported only in a few families. The disease is...

Inherited renal tubular disorders associated With hypokalemic alkalosis (Bartter-like syndromes) can be subdivided into at least three clinical phenotypes: (i) the hypocalciuric-hypomagnesemic Gitelman variant; (ii) the classic variant; and (iii) the antenatal hypercalciuric variant (also termed hyperprostaglandin E syndrome). Mutations in the Na-C...