Linh Nguyen

University of Melbourne | MSD · Department of Surgery

Background: The Kallikrein-Kinin System (KKS) has been found to play a role in tumor progression in several cancers. The KKS metabolic cascade depends on signalling through two cross talking receptors; bradykinin receptor 1 (B1R) and bradykinin receptor 2 (B2R). Activation of the Kinin receptor is responsible for multiple pathophysiologic function...

Background: Preclinical research indicate that vascular disrupting agent (VDA) treatment induces extensive tumor death but also a systemic mobilization of bone marrow derived cells including endothelial progenitor cells (EPC) leading to revascularization and renewed growth within the residual tumor. This study investigates if combination of VDA wi...

Background and aim: Epithelial to mesenchymal transition (EMT) is implicated in tumour progression. We aimed to determine if the renin angiotensin system (RAS) has a role in colorectal cancer (CRC) cell EMT. Methods: Human CRC cell lines DLD-1 and LIM2405 used in wound scratch migration assays where they were treated with RAS peptide ANG II alon...

Background. Colorectal Cancer (CRC) is the most common form of cancer diagnosed in Australia across both genders. Approximately, 40%-60% of patients with CRC develop metastasis, the liver being the most common site. Almost 70% of CRC mortality can be attributed to the development of liver metastasis. This study examines the pattern and density of l...

Temporal changes in VEGF and AT1R expression following OXi4503 treatment determined by immuno histochemistry. Tissues were collected at indicated times following OXi4503 treatment. Expression (brown staining) was detected using the appropriate antibodies. Black bar = 200 μm; L, liver; T, tumor; N, treatment induced necrosis. Single red arrows indic...

Changes in caspase 3 staining of CRCLM tumors following OXi4503 treatment. Mice with liver metastases were treated with a single IP dose of OXi4503 (100 mg/kg) at 16 days post tumor induction. Tissues were collected at indicated times following OXi4503 treatment. First column, black bar = 250 μm, second column, black bar = 50 μm, third column = mag...

List of antibodies and conditions used.

Temporal changes in proangionenic growth factor concentration in liver lysates and serum following OXi4503 treatment. Tissues were collected at indicated times following OXi4503 treatment. Growth factor concentration was determined in lysates by capture ELISA assays using commercially available kits (R&D Duo Kits) ▪, control tumor bearing mice; □,...

Changes in EMT markers following OXi4503 treatment of tumor metastases. Mice with liver metastases were treated with a single IP dose of OXi4503 (100 mg/ kg) at 16 days post tumor induction. Tissues were collected at 1, 24 h and 5 days following OXi4503 treatment. Formalin fixed control and treated tumor sections in columns 1–4 were stained with an...

Epithelial to mesenchymal transition (EMT) is considered an important mechanism in tumor resistance to drug treatments; however, in vivo observation of this process has been limited. In this study we demonstrated an immediate and widespread EMT involving all surviving tumor cells following treatment of a mouse model of colorectal liver metastases w...

Hepatic resection remains the treatment of choice for patients with colorectal liver metastases (CLM). Indications for hepatic resection have been extended to include extrahepatic lymph node groups, resulting in increased survival benefits. The identification of specific lymph pathways and involved nodes is necessary to support the development of g...

Background Treatment of solid tumors with vascular disrupting agent OXi4503 results in over 90% tumor destruction. However, a thin rim of viable cells persists in the tumor periphery following treatment, contributing to subsequent recurrence. This study investigates inherent differences in the microenvironment of the tumor periphery that contribute...

Preliminary experimental studies have suggested that the in situ destruction of tumor tissue by local laser ablation (LA) may also stimulate host immunity against cancer. We investigated local and systemic induction of immune responses after laser ablation in the setting of residual tumor. A murine colorectal cancer (CRC) liver metastasis model was...

Figure S1: Temporal changes in CD3+ T cells and Kupffer cells in the liver parenchyma distant from injury sites following liver LA treatment. Groups of mice (n >5) had LA treatment in the liver and tissue was collected at various time points post ablation as indicated. Control mice had sham LA performed (LA probe was not activated), black bars = sh...