Lingyu Guan

Rutgers, The State University of New Jersey | Rutgers · Center for Computational and Integrative Biology (CCIB)

tRNA-derived fragments (tRFs) are a class of emerging post-transcriptional regulators of gene expression likely binding to the transcripts of target genes. However, only a few tRFs targets have been experimentally validated, making it hard to extrapolate the functions or binding mechanisms of tRFs. The paucity of resources supporting the identifica...

Controversial reports of human-virus chimeric reads (HVCRs) suggested a possible integration of SARS-CoV-2 sequences into human DNA (1-3).…

Accumulating evidence has suggested that tRNA-derived fragments (tRFs) could be loaded to Argonaute proteins and function as regulatory small RNAs. However, their mode of action remains largely unknown, and investigations of their binding mechanisms have been limited, revealing little more than microRNA-like seed regions in a handful of tRFs and a...

The most abundant cellular RNA species, ribosomal RNA (rRNA), appears to be a source of massive amounts of non-randomly generated fragments. We found rRNA fragments (rRFs) in immunoprecipitated Argonaute (Ago-IP) complexes in human and mouse cells and in small RNA sequencing datasets. In human Ago1-IP, guanine-rich rRFs were preferentially cut in s...

Transfer RNA and ribosomal RNA are known for their traditional roles in translation. Here we reviewed evidence of their novel functionality as sources of short RNA fragments and the emerging role of these fragments as posttranscriptional regulators, including neuronal and aging processes. Such transfer RNA and ribosomal RNA fragments (tRFs and rRFs...

Transfer RNA fragments (tRFs) are an emerging class of small RNA molecules derived from mature or precursor tRNAs. They are found across a wide range of organisms and tissues, in small RNA fraction or loaded to Argonaute in numbers comparable to microRNAs. Their functions and mechanisms of action are largely unknown, and results obtained on individ...

Background Accumulating evidence is pointing to functional roles of rRNA derived fragments (rRFs), often considered degradation byproducts. Small RNAs, including miRNAs and tRNA-derived fragments (tRFs), have been implicated in the aging process and we considered rRFs in this context. Objective We performed a computational analysis of Argonaute-lo...

Background A number of studies have associated obesity with altered gut microbiota, although results are discordant regarding compositional changes in the gut microbiota of obese animals. Herein we aim to obtain an unbiased evaluation of structural and functional changes of the gut microbiota of diet‐induced obese rodents using a meta‐analysis. Me...

Beneficial effects of the Chinese herbal medicine Qushi Huayu Decoction (QHD) were observed with non-alcoholic fatty liver disease (NAFLD) patients and animal models. The impact of QHD or its active components (geniposide and chlorogenic acid, GC) on NAFLD liver transcriptome and gut microbiota was examined with NAFLD rats. Increased expression for...

Clinical studies have demonstrated the therapeutic efficacy of the Chinese herbal medicine Qushi Huayu Decoction (QHD) in the treatment of non‐alcoholic fatty liver disease (NAFLD). It has been suggested that QHD reduces hepatic lipid accumulation through its regulatory effect on liver fat metabolism. Herein, to better understand the pharmaceutical...