Lingwen Zeng

Chinese Academy of Sciences | CAS · Stem Cell and Molecular Diagnostics

The preparation of platinum-based catalyst materials with high activity and stability for direct methanol fuel cells (DMFCs) commercial process has significant significance. Zeolite imidazole skeleton (ZIF-8) as the precursor, through high temperature carbonization to prepare an excellent metal organic frameworks (MOFs) derived carbon materials as...

In this study, a screen-printed carbon electrode (SPCE) based on in situ deposition modification was developed for the sensitive, rapid, easy and convenient determination of As(III) in water and tea by linear sweep anodic stripping voltammetry (LSASV). The screen-printed carbon electrodes were placed in a solution consisting of As(III) solution, ch...

Pure inorganic perovskite materials have been widely investigated due to their color tunability, narrow band gap, simple fabrication process, and low cost. However, their application is limited by their instability in aqueous and oxygen environments. Herein, to improve the stability of perovskite quantum dots in aqueous and oxygen environments, CsP...

Coronavirus disease 2019 (COVID-19) caused by the SARS-CoV-2 virus has led to a global pandemic with a high spread rate and pathogenicity. Thus, with limited testing solutions, it is imperative to develop early-stage diagnostics for rapid and accurate detection of SARS-CoV-2 to contain the rapid transmission of the ongoing COVID-19 pandemic. In thi...

Cross-border pathogens such as the African swine fever virus (ASFV) still pose a socioeconomic threat. Cheaper, faster, and accurate diagnostics are imperative for healthcare and food safety applications. Currently, the discovery of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) has paved the way for the diagnostics based on...

The pathogen of viral hemorrhagic fever (VHF), which is harmful to human health, is a hemorrhagic fever virus. Clinicians have long needed convenient and sensitive point-of-care rapid diagnostic tests (RDTs) for hemorrhagic fever viruses. Commonly used methods for pathogen detection rely on conventional culture-based tests, antibody-based assays an...

Nasopharyngeal carcinoma (NPC) is one of the most common malignant tumors in the world, and several studies have associated Epstein–Barr virus (EBV) with NPC occurrence and development. EBV-PCR (polymerase chain reaction), in situ hybridization and immunoassays are the most common methods for NPC identification. However, these approaches have drawb...

The advancement in developing sensitive, rapid, and specific sensing tools is crucial in diagnostics and biotechnological applications. Although various isothermal amplification approaches exist for the detection and identification of nucleic acids, post-amplicon analysis is still based on traditional methods such as gel electrophoresis, colorimetr...

Nervous necrosis virus (NNV) has infected more than 50 fish species worldwide, and has caused serious economic losses in the aquaculture industries. However, there is no effective antiviral therapy. The development of a rapid and accurate point-of-care diagnostic method for the prevention and control of NNV infection is urgently required. Commonly...

Abnormal growth of uterine cervix cells may eventually lead to cervical cancer, which is mainly associated with the two most prevalent types of Human Papilloma Virus (HPV), namely HPV16 and HPV18. Cervical cancer can be prevented or treated owing to its slow progression.Therefore, its early, fast, and sensitive diagnosis is crucial. Herein, we deve...

CRISPR/Cas systems have displayed remarkable potential in developing novel biosensing applications for nucleic acid detection owing to the collateral cleavage activity of Cas effector proteins (Cas12, Cas13, etc.). Despite tremendous progress in recent years, the existing CRISPR/Cas based biosensing platforms have several limitations, including rel...

The rapid determination of human osteopontin (OPN) protein, a potential cancer biomarker, holds substantial promise for point-of-care diagnostics and biomedical applications. To date, most reported platforms for OPN detection are apparatus-dependent, time-consuming, and expensive. Herein, we established a lateral flow biosensor (LFB) for OPN detect...

The identification of various targets such as bacteria, viruses, and other cells remains a prerequisite for point-of-care diagnostics and biotechnological applications. Nucleic acids, as encoding information for all forms of life, are excellent biomarkers for detecting pathogens, hereditary diseases, and cancers. To date, many techniques have been...

Vanillin (4-hydroxy-3-methoxybenzaldehyde), a food additive with rich milk flavor, is commonly used in the food, beverage and cosmetic industries. However, excessive consumption of vanillin may cause liver and kidney damage. Therefore, methods for detecting and controlling the level of vanillin in food, especially in infant powder, have important p...

The UV-vis absorption curve data of Fig 5. Milk powder samples compared with vanillin standard sample (50 μg mL-1). (DOCX)

The UV-vis absorption curve data of Fig 2A. Spectra of four kinds of acid buffer. (DOCX)

The UV-vis absorption curve data of Fig 2B. Spectra of all reagents. (DOCX)

The UV-vis absorption curve data of Fig 2E. Spectra of standard samples from 1 μg mL−1 to 100 μg mL−1 before heating. (DOCX)

The UV-vis absorption curve data of Fig 2F. Spectra of standard samples from 1 μg mL−1 to 100 μg mL−1 after heating. (DOCX)

Measurements details data of vanillin spiked in milk powder samples (n = 6). (DOCX)

The UV-vis absorption curve data of Fig 2C. Spectra of standard samples from 1 μg mL−1 to 100 μg mL−1 before heating. (DOCX)

The UV-vis absorption curve data of Fig 2D. Spectra of standard samples from 1 μg mL−1 to 100 μg mL−1 after heating. (DOCX)

The UV-vis absorption curve data of Fig 3. The absorption spectra of the vanillin samples from 1 μg mL−1 to 500 μg mL−1. (DOCX)

Objective: To investigate the rapid and accurate method for the detection of dengue virus (DENV) by using nicking enzyme assisted strand-displacement amplification (SDA) combined with gold nanoparticles-based lateral flow strip. Methods: Total RNA of the virus was extracted by using magnetic beads method and transcribed to cDNA for SDA detection...

The analysis of intracellular ATP can reveal the response of cells to different treatments and is important for individualized medicine. In the present study, we developed a cell penetrating peptides (CPPs) tagged luciferase (TAT-LUC) for tumor chemosensitivity assay. The activity of recombinant TAT-LUC was evaluated using ATP standard solution and...

Sequencing results: TAT-LUC sequence. Red represents the TAT tag. (DOCX)

A lateral flow biosensor (LFB) is introduced for the detection of single nucleotide polymorphisms (SNPs). The assay is composed of two steps: circular strand displacement reaction and lateral flow biosensor detection. In step 1, the nucleotide at SNP site is recognized by T4 DNA ligase and the signal is amplified by strand displacement DNA polymera...

The use of potential mutagenic nitrofuran antibiotic in food animal production has been banned world-wide. Common methods for nitrofuran detection involve complex extraction procedures. In the present study, magnetic beads functionalized with antibody against nitrofuran derivative were used as both the extraction and color developing media in later...

The umbilical cord contains different populations of stem/progenitor cells such as mesenchymal, hematopoietic, trophoblastic and endothelial cells. However, the isolation methods currently in use are different, and the resulted cells are liable to cross contamination. Thus it is increasingly difficult to compare and contrast the study outcomes, whi...

Copper(ii) ions (Cu2+) at a high concentration are harmful to human health. Herein a simple and enzyme-free lateral flow biosensor for the rapid detection of Cu2+ based on copper(i) ion (Cu+)-catalyzed click chemistry has been constructed for the first time. In the presence of sodium ascorbate, Cu2+ was reduced to Cu+, which could catalyze the cycl...

The mouse is an organism that is widely used as a mammalian model for studying human physiology or disease, and the development of immunodeficient mice has provided a valuable tool for basic and applied human disease research. Following the development of large-scale mouse knockout programs and genome-editing tools, it has become increasingly effic...

Human pluripotent stem cells (hPSCs), such as embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), provide a powerful model system for studies of cellular identity and early mammalian development, which hold great promise for regenerative medicine. It is necessary to develop a convenient method to discriminate hPSCs from other ce...

Foodborne diseases caused by pathogens are one of the major problems in food safety. Convenient and sensitive point-of-care rapid diagnostic tests for food-borne pathogens have been a long-felt need of clinicians. Commonly used methods for pathogen detection rely on conventional culture-based tests, antibody-based assays and polymerase chain reacti...

A novel glucometer biosensor was developed for the quantitative detection of mercury ions (Hg(2+)) based on glucose-loaded DNA-gated mesoporous silica nanoparticles (MSNs).

A universal biosensor for the portable and quantitative detection of transcription factors has been constructed using a commercially available glucometer as the sensing platform. With the specific protein-binding DNA and antibody as the recognition elements, invertase as the linker, and glucometer as the transducer, quantitative detection was achie...

G-quadruplex-forming sequence can be formed through a copper(I) ion (Cu(+))-catalyzed click chemistry between azide- and alkyne-modified short G-rich sequences in aqueous solution, eliminating immobilization and washing steps of conventional assays. The source for Cu(+) was generated from the reduction of Cu(2+) with the reductant of sodium ascorba...

In this communication, we report a rapid and cost-efficient assay for the detection of S. enteritidis. Specific aptamers with fluorescence labeled S. enteritidis are absorbed into graphene oxide, and the fluorescence is quenched owing to the fluorescence quenching ability of graphene oxide. In the presence of S. enteritidis, the aptamer would relea...

An autonomous thymine rich DNA machine as an amplification unit was developed for the sensitive detection of mercury ions with high specificity. Combined with a lateral flow biosensor, the amplified signal of Hg2+ can be read out by the naked eye with a detection limit of 5 nM.

Inhibition of inosine monophosphate dehydrogenase (IMPDH) by mycophenolic acid (MPA) can inhibit proliferation and induce apoptosis in cancer cells. This study investigated the underlying molecular mechanisms of MPA's anticancer activity. A gastric cancer cell line (AGS) was treated with MPA and gene expression at different time points was analyzed...

Mycophenolate mofetil (MMF), the prodrug of mycophenolic acid (MPA) which has been widely used for the prevention of acute graft rejection, is a potent inhibitor of inosine monophosphate dehydrogenase (IMPDH) that is up-regulated in many tumors and potentially a target for cancer therapy. MPA is known to inhibit cancer cell proliferation and induce...

Mycophenolic acid (MPA) is the metabolized product and active element of mycophenolate mofetil (MMF) that has been widely used for the prevention of acute graft rejection. MPA potently inhibits inosine monophosphate dehydrogenase (IMPDH) that is up-regulated in many tumors and MPA is known to inhibit cancer cell proliferation as well as fibroblast...

We demonstrated a new spectrophotometric DNA detection approach based on a circular strand-displacement polymerization reaction for the quantitative detection of sequence specific DNA. In this assay, the hybridization of an immobilized hairpin probe on the microtiter plate, to target DNA, results in a conformational change and leads to a stem separ...

The detection of nucleic acid is of central importance for the diagnosis of genetic diseases, infectious agents, and biowarfare agents. Traditional strategies and technologies for nucleic acid detection are time-consuming and labor-intensive. Recently, isothermal strand-displacement reaction-based lateral flow biosensors have attracted a great deal...

A rapid and reliable screening test for thalassemia carrier couples is the most effective strategy to decrease the risk of conceiving fetuses with severe thalassemia. We present an approach based on the isothermal strand-displacement polymerase reaction and the use of a lateral flow strip for the visual detection of an α-thalassemia Southeast Asian...

Histone methylation is a crucial epigenetic modification of chromosomes. In this work, we describe an enhanced strip biosensor using oligonucleotide functionalized gold nanoparticles as an enhancer probe (AuNP-DNA) for rapid and sensitive detection of histone methylation. In conventional strip biosensor, methylated histone is captured on the test zo...

An enzyme-free and label-free fluorescence turn on biosensor for amplified copper(ii) ion (Cu(2+)) detection has been constructed based on self-assembled DNA concatamers and Sybr Green I. This assay is simple, inexpensive and sensitive, enabling quantitative detection of as low as 12.8 pM Cu(2+).

A direct ELISA was established for the fast detection of semicarbazide (SEM) using a novel biotin derivative. Without a tedious extraction procedure, as low as 0.07 μg L(-1) of SEM could be detected reproducibly. This assay has better recovery and accuracy than competitive ELISA.

A hairpin DNA probe mediated cascade signal amplification method was developed for visual and rapid DNA analysis with a detection limit of 100 aM. The implementation of tag/anti-tag DNA and gold nanoparticle reporters permits a universal platform for multiplex genotyping without instrumentation.

A simple and enzyme-free strip biosensor for the amplified detection of Pb(2+) has been constructed based on a catalytic DNA circuit. This assay is ultrasensitive, enabling the visual detection of Pb(2+) concentrations as low as 10 pM without instrumentation.

A simple and rapid method for the detection of S(2-) anions with high selectivity and sensitivity has been developed by using random double-strand DNA-templated formation of copper nanoparticles as novel fluorescence probes in aqueous solution.

A series of enzyme-amplified electronic logic gates (OR, AND, NOR, and NAND) for one-spot simultaneous monitoring of small molecules and proteins has been constructed at the molecular level. This simple but universal system is based on target-induced self-assembly of split aptamer fragments or target-induced conformational changes of intact aptamer...

A double-stranded DNA (dsDNA) mediated sandwich assay was developed for quantitative detection of transcription factors. The detection limit for human recombinant c-jun protein is 2.5 ng, and for c-jun protein the limit is as low as 0.625 μg of cell lysate.

A lateral flow biosensor for detection of single nucleotide polymorphism based on circular strand displacement reaction (CSDPR) has been developed. Taking advantage of high fidelity of T4 DNA ligase, signal amplification by CSDPR, and the optical properties of gold nanoparticles, this assay has reached a detection limit of 0.01 fM.

The first example of strip logic gates ("OR" and "AND" functions) for proteins and small molecules has been constructed on the basis of target-induced self-assembly of split aptamer fragments. Using thrombin and ATP as inputs, the corresponding split/integrated aptamers as molecular recognition elements, and gold nanoparticles as a tracer, the outp...

In this work, we describe a simple colorimetric method to detect DNA methylation. Adenomatous polyposis coli (APC) with a small CpG region containing methylated cytosine (methylated APC) was synthesized and tested. Methylated APC was first captured and enriched by anti-5-methylcytosine monoclonal antibody conjugated magnetic microspheres (MMPs). Th...

A simple label-free method for the detection of Pb(2+) ions with high selectivity and sensitivity has been developed by using random double-strand DNA-templated formation of copper nanoparticles as novel fluorescence probes in aqueous solution.

Reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) resets the epigenome to an embryonic-like state. Vitamin C enhances the reprogramming process, but the underlying mechanisms are unclear. Here we show that the histone demethylases Jhdm1a/1b are key effectors of somatic cell reprogramming downstream of vitamin C. We first ob...

A lateral flow biosensor based on isothermal strand-displacement polymerase reaction and gold nanoparticles has been developed for the visual detection of nucleic acids with a detection limit of 0.01 fM.

A lateral flow biosensor based on an immuno-chromatographic assay has been developed for the detection of DNA-binding proteins. The biosensor is composed of four parts: a sample pad, a conjugate pad, a strip of nitrocellulose membrane and an absorbent pad. A DNA probe containing a specific protein binding consensus sequence is coated onto gold nano...

Generation of induced pluripotent stem cells by defined factors has become a useful model to investigate the mechanism of reprogramming and cell fate determination. However, the precise mechanism of factor-based reprogramming remains unclear. Here, we show that Klf4 mainly acts at the initial phase of reprogramming to initiate mesenchymal-to-epithe...

Vascular endothelial growth factor (VEGF) is one of the best characterized angiogenic factors controlling placental angiogenesis; however, how VEGF regulates placental angiogenesis has not yet completely understood. In this study, we found that all the components of assembling a functional NADPH oxidase (NOX2, p22(phox), p47(phox), p67(phox), and R...