About
38
Publications
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Introduction
Current institution
Additional affiliations
March 2022 - July 2022
Texas Children's Hospital, Baylor College of Medicine
Position
- Instructor
June 2016 - March 2022
Texas Children's Hospital, Baylor College of Medicine
Position
- PostDoc Position
March 2015 - June 2016
Publications
Publications (38)
MYCN activation is a hallmark of advanced neuroblastoma (NB) and a known master regulator of metabolic reprogramming, favoring NB adaptation to its microenvironment. We found that the expression of the main regulators of the molecular clock loops is profoundly disrupted in MYCN-amplified NB patients, and this disruption independently predicts poor...
Neuroblastoma (NB) arises from oncogenic disruption of neural crest (NC) differentiation. Treatment with retinoic acid (RA) to induce differentiation has improved survival in some NB patients, but not all patients respond, and most NBs eventually develop resistance to RA. Loss of the chromatin modifier chromatin assembly factor 1 subunit p150 (CHAF...
Neuroblastoma (NB) is a childhood cancer arising from sympatho-adrenal neural crest cells. MYCN amplification is found in half of high-risk NB patients; however, no available therapies directly target MYCN. Using multi-dimensional metabolic profiling in MYCN expression systems and primary patient tumors, we comprehensively characterized the metabol...
Continuous monitoring and closed-loop therapy of soft wound tissues is of particular interest in biomedical research and clinical practices. An important focus is on the development of implantable bioelectronics that can measure time-dependent temperature distribution related to localized inflammation over large areas of wound and offer in situ tre...
Several cancer core regulatory circuitries (CRCs) depend on the sustained generation of DNA accessibility by SWI/SNF chromatin remodelers. However, the window when SWI/SNF is acutely essential in these settings has not been identified. Here we used neuroblastoma (NB) cells to model and dissect the relationship between cell-cycle progression and SWI...
Diet dictates nutrient availability in the tumor microenvironment, thus affecting tumor metabolic activity and growth. Intrinsically, tumors develop unique metabolic features and are sensitive to environmental nutrient concentrations. Tumor-driven nutrient dependencies provide opportunities to control tumor growth by nutritional restriction or supp...
MYCN is a major driver for neuroblastoma (NB) and the tyrosine hydroxylase (TH)-MYCN transgenic mouse model is extensively used for preclinical NB studies. However, spatio-temporal NB progression in the TH-MYCN model has not been studied, and questions remain about the value of implanted models as a surrogate for transgenic mice. In this work, we u...
Background:
Avocados are a nutrient-dense source of MUFAs and are rich in antioxidants. Avocados have an additional LDL cholesterol (LDL-C) lowering effect beyond that observed when their MUFAs are substituted for SFAs, especially on small, dense LDL (sdLDL) particles, which are susceptible to in vivo oxidation and associated with increased risk o...
Background:
We previously showed enrichments of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) in broiler chicks fed defatted microalgae.
Objectives:
The aims of this study were to determine 1) if the enrichments affected meat texture and were enhanced by manipulating dietary corn oil, selenium, and vitamin E concentrations and 2) ho...
Regorafenib suppresses RET protein levels and abrogates glucose metabolism in NB cells
Neuroblastoma (NB), the most common extracranial pediatric solid tumor,
continues to cause significant cancer-related morbidity and mortality in children.
Dysregulation of oncogenic receptor tyrosine kinases (RTKs) has been shown to
contribute to tumorigenesis in various human cancers and targeting these RTKs has
had therapeutic benefit. RET is an...
Purpose:
mTORC1 inhibitors are promising agents for neuroblastoma therapy, however they have shown limited clinical activity as monotherapy, thus rational drug combinations need to be explored to improve efficacy. Importantly, neuroblastoma maintains both an active p53 and an aberrant mTOR signaling.
Experimental Design: Using an orthotopic xeno...
Background. MYCN activation is a hallmark of advanced tumor stage in neuroblastoma (NB), characterizing high-risk patients prone to resistant disease. MYCN is also a potent regulator of metabolic reprogramming that favors NB adaptation to its microenvironment. The retinoic acid receptor-related orphan receptor α (RORα) is a key regulator of cell me...
Neuroblastoma (NB) is the most common extracranial solid tumor in children. Aberrant activation of the non-receptor tyrosine kinases Src and c-Abl contributes to the progression of NB. Thus, targeting these kinases could be a promising strategy for NB therapy. In this paper, we report that the potent dual Src/Abl inhibitor bosutinib exerts anti-tum...
Neuroblastoma is the most common extracranial solid tumor in children. The ErbB family of proteins is a group of receptor tyrosine kinases that promote the progression of various malignant cancers including neuroblastoma. Thus, targeting them with small molecule inhibitors is a promising strategy for neuroblastoma therapy. In this study, we investi...
Whereas a number of studies have examined the effects of soy isoflavones and tocopherols on colonic inflammation, few have examined soy protein. We determined the radical scavenging and cytoprotective effects of soy protein concentrate (SPC) in vitro and its anti-inflammatory effects in dextran sulfate sodium (DSS)-treated mice. Cotreatment with SP...
Effects of defatted microalgae (DFA) on fatty acid composition of eggs and tissues of hens have not been well studied. This experiment was to determine how low concentrations of supplemental DFA in the presence or absence of flaxseed oil (FO) altered fatty acid profiles of egg yolk, liver, adipose tissue, and plasma of layers. A total of 60 Shaver...
Two experiments were conducted to determine effects of supplementing flaxseed oil (FO) and microalgae alone or in combination in diets for hens (Shaver Leghorn) on enriching egg n − 3 fatty acids. Experiment I was a 3 × 3 factorial arrangement of treatments (0, 3, and 5% FO and 0, 7.5, and 10% full-fatted Staurosira sp.). Experiment II consisted of...
Glutathione peroxidase‐1 (GPX1) and Cu/Zn‐superoxide dismutase (SOD1) are two major antioxidant enzymes, while polyunsaturated fatty acids (PUFA) including eicosapentaenoic acid (20:5n‐3, EPA) and docosahexaenoic acid (22:6n‐3, DHA) are health‐promoting nutrients. This study was conducted to determine the effects of GPX1 overexpression and SOD1 kno...
Our laboratory has revealed novel roles and mechanisms of glutathione peroxidase-1 (GPX1) in glucose and lipid metabolism, whereas little is known about its function in protein metabolism. This study was conducted to elucidate the physiological impacts of the GPX1 overexpression and knockout on gene expression and activity of key factors related to...
Our laboratory has revealed novel roles and mechanisms of glutathione peroxidase‐1 (GPX1) in glucose and lipid metabolism, whereas little is known about its function in protein metabolism. This study was conducted to elucidate the physiological impacts of the GPX1 overexpression and knockout on gene expression and activity of key factors related to...
For many years, both preclinical and clinical studies have provided evidences to support the beneficial effects of ω-3 Polyunsaturated fatty acids (PUFAs), particularly Eicosapen-taenoic acid (EPA) and Docosahexaenoic acid (DHA) in the prevention of chronic diseases. However, recently, an increasing number of studies reported adverse or contradicto...
The tea catechin, (‐)‐Epigallocatechin‐3‐gallate (EGCG), has been shown to exert cancer preventive effects. We and others have found that EGCG induces oxidative stress in oral cancer cells but causes antioxidant responses in normal cells. Here, we examined the role of antioxidant protein metallothionein (MT) in the differential pro‐oxidant effects...
Nonalcoholic fatty liver disease (NAFLD) is an important co‐morbidity of obesity. Previously, we found that dietary cocoa (Theobroma cacao) supplementation reduced hepatic lipid content and inflammation in obese mice. Here, we investigated the effect of dietary cocoa on hepatic mitochondrial biogenesis, oxidative stress, and antioxidant response. M...
Scope:
We have previously reported that the green tea catechin, (-)-epigallocatechin-3-gallate (EGCG), can induce oxidative stress in oral cancer cells but exerts antioxidant effects in normal cells. Here, we report that these differential prooxidative effects are associated with sirtuin 3 (SIRT3), an important mitochondrial redox modulator.
Meth...
Abstract Text: The objective of this experiment was to investigate the effects of dietary Capsicum extract (CE) on T-cell phenotypes, phagocytotic and oxidative burst activity of neutrophils, blood cell counts, blood chemistry, and oxidative stress markers in lactating dairy cows. Eight multiparous Holstein cows (DIM, 50 ± 9.6 d; BW, 591 ± 32.6 kg)...
The tea catechin, (-)-epigallocatechin-3-gallate (EGCG), has potential cancer preventive effects. The prooxidant activity of EGCG may play a role in these effects.
Here, we report that EGCG exerted cytotoxic effects against oral cancer cell lines (IC50 = 83-95 μM). EGCG treatment resulted in formation of extracellular reactive oxygen species (ROS),...
Dietary approaches may play an important role in reducing oral cancer burden. The green tea catechin, (‐)‐epigallocatechin‐3‐gallate (EGCG), has been reported to have cancer preventive effects. Previously, we found that EGCG induced mitochondrial dysfunction, oxidative stress and apoptosis in oral cancer cells but exerted antioxidant effects in nor...
Green tea (Camellia sinensis) is a widely consumed beverage that has shown preventive effects against chronic diseases including cancer. Green tea polyphenols are believed to be responsible for this cancer preventive effect and modulation of redox activity has been suggested as a potential mechanism of action. Data from various laboratories has sug...
(-)-Epigallocatechin-3-gallate (EGCG) is the most abundant polyphenol in green tea (Camellia sinensis). Although many studies have demonstrated its cancer chemopreventive and chemotherapeutic effects, the potential for development of EGCG resistance in cancer cells has not been well-studied. The purpose of this study was to develop and characterize...
(-)-Epigallocatechin-3-gallate (EGCG) is the most abundant polyphenol in green tea (Camellia sinensis). Many laboratory studies have demonstrated its potential cancer chemopreventive effects. Previously, we found that EGCG inhibited cell growth and induced apoptosis in oral squamous carcinoma cells (SCC-25 and SCC-9), and that, EGCG-induced intrace...
(-)-Epigallocatechin-3-gallate (EGCG) is the most abundant polyphenol in green tea (Camellia sinensis L.). Recently, it has been reported to have cancer chemotherapeutic and chemopreventive effects. However, the mechanisms are incompletely understood. We found that EGCG induced both extracellular and intracellular reactive oxygen species (ROS) in S...
An early light-inducible protein gene (CaELIP) was isolated from a cDNA library of hot pepper (Capsicum annuum) that showed heavy metal stress-inducible expressions. This gene contains an open reading frame (ORF) encoding a protein of 160 amino acids, and the protein has significant homology with reported early light-inducible proteins from other p...
Cassia tora is an annual legume and cultivated as a traditional medicinal herb for multiple therapies including regulation of blood pressure and blood lipid. Because of naturally occurring acidic soils in southeastern China, this plant species may possess strategies for tolerance to low pH and aluminum toxicity. In the search for the regulatory bas...
Questions
Questions (4)
Dear colleagues,
I constructed a protein expression vector. After transfection, my target protein and linked GFP was well expressed. However, the protein was dispersed everywhere in the cells, making quantitative imaging difficult. I would like to control the target protein to a low amount in cells. Do you know if there are any methods to reduce the protein expression in cells? For example, adding some inhibitor at certain time?
Thank you,
Ling
For GC, the actual make up gas flow is zero. Yet, there is gas pressure from the tank. May I know what happened here? Because there is no make up gas, the machine is not ready.
Sirtuin family proteins (SIRTs) have been widely studied recently. I found a lot of research on the function of SIRTs but no information (within my searching ability) on the regulation of SIRTs (particularly SIRT3) ACTIVITY. Is anyone working on this or interested in working on it? I think this will be very helpful for the understanding of upstream signaling of SIRTs.
I am interested in doing cancer stem cell research in the future. I would like to get some information to help me find the suitable lab. Thanks!
Thanks for every response!
