Linda Sommese

Linda Sommese
Università degli Studi della Campania "Luigi Vanvitelli · Department of Environmental, Biological and Pharmaceutical Sciences and Technologies

BSc

About

108
Publications
16,091
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1,627
Citations
Citations since 2016
17 Research Items
1039 Citations
2016201720182019202020212022050100150
2016201720182019202020212022050100150
2016201720182019202020212022050100150
2016201720182019202020212022050100150
Additional affiliations
January 2015 - present
Università degli Studi della Campania "Luigi Vanvitelli
Position
  • Senior Researcher

Publications

Publications (108)
Article
Full-text available
Background DNA methylation can play a pathogenic role in the early stages of hyperglycemia linking homeostasis imbalance and vascular damage. Material and methods We investigated DNA methylome by RRBS in CD04⁺ and CD08⁺ T cells from healthy subjects (HS) to pre-diabetics (Pre-Diab) and type 2 diabetic (T2D) patients to identify early biomarkers of...
Preprint
Background: Despite current intensive treatments, hyperglycemic patients have an unfavorable prognosis due to severe CHD and development of complications. The interplay between hyperglycemia and systemic inflammation can modify patterns of gene expression mainly affecting DNA methylation in promoter regions and, thus, endothelial damage. However, D...
Article
Full-text available
Introduction: Blood transfusion is a lifesaving procedure for patients affected by hematological diseases or hemorrhage risk. Aim: This retrospective study was aimed to evaluate clinical safety of pediatric transfusions by comparing the frequency of adverse events caused by apheretic blood components vs whole blood. Methods: From 2011 to 2015,...
Article
Introduction: Human leukocyte antigen-G (HLA-G) gene encodes for a tolerogenic molecule constitutively expressed in human pancreas and up-regulated upon inflammatory signals. The 14 bp INS/DEL polymorphism in the 3ʹUTR of HLA-G may influence the susceptibility for diabetes and coronary artery diseases (CHD), thus suggesting a novel candidate gene....
Article
Background: Epigenetics modulated tissue-specific gene expression during the onset of type 1 and type 2 diabetes and their complications. Methods: We searched the PubMed recent studies about the main epigenetic tags involved in type 1 and type 2 diabetes onset and their clinical complications. PubMed studies about the epigenetic tags involved in...
Article
Purpose: Human leukocyte antigen (HLA)-G is a non-classic major histocompatibility complex HLA class I molecule. HLA-G may have tolerogenic properties which are linked to epigenetic-sensitive pathways. There is a correlation of sHLA-G levels and graft acceptance in transplantation studies. There are previous data on correlation of sHLA-G with graf...
Article
Full-text available
Background: Patients with end-stage liver disease (ESLD) have a compromised nutritional status because of the liver crucial role in regulating metabolic homeostasis and energy balance. Data sources: A systematic review of literature based on extensive relevant articles published from 2001 to 2017 in English in PubMed database was performed by se...
Article
Full-text available
Prevention of alveolar bone resorption after tooth extraction may be useful for implant rehabilitation of the edentulous site minimizing the future need for bone augmentation procedures. A number of studies have investigated the efficacy of autologous platelet concentrates for the preservation of the alveolar bone volume after tooth extraction. Alt...
Article
Full-text available
HBV still represents a global risk factor in transfusion medicine. The residual risk of HBV is not limited to pre-seroconversion window period but it extends to donors with occult HBV infection (OBI) characterized by the presence of HBV DNA in liver and by the absence of the virus surface antigen. Each country developed an appropriate blood screeni...
Article
Type 1 diabetes (T1D) is an irreversible degenerative disease with severe complications such as heart disease, nephropathy, neuropathy, and retinopathy. Although exogenous insulin administration is a life-saving therapy, it does not cure the disease. This review addresses the epigenetic mechanisms responsible for the development of T1D and discusse...
Article
Full-text available
Epigenetics is involved in the altered expression of gene networks that underlie insulin resistance and insufficiency. Major genes controlling β-cell differentiation and function, such as PAX4, PDX1, and GLP1 receptor, are epigenetically controlled. Epigenetics can cause insulin resistance through immunomediated pro-inflammatory actions related to...
Article
Full-text available
The regenerative potential of tissues and organs could promote survival, extended lifespan and healthy life in multicellular organisms. Niches of adult stemness are widely distributed and lead to the anatomical and functional regeneration of the damaged organ. Conversely, muscular regeneration in mammals, and humans in particular, is very limited a...
Article
Full-text available
Background: Circulating endothelial progenitors cells (EPCs) play a critical role in neovascularization and endothelial repair. There is a growing evidence that hyperglycemia related to Diabetes Mellitus (DM) decreases EPC number and function so promoting vascular complications. Aim of the study: This study investigated whether an intensive glyc...
Article
Full-text available
Objective: To report on the clinical benefits of platelet gel application in a non-regenerating skin wound. Clinical presentation and intervention: An 84-year-old man presented with a severe wound with a regular circumference in the frontal region which resulted in a complete loss of epidermis and dermis. The skin lesion, induced by cryosurgery...
Article
Full-text available
Epigenetic modifications include DNA methylation, histone modifications, and microRNA. Gene alterations have been found to be associated with cardiovascular diseases, and epigenetic mechanisms are continuously being studied to find new useful strategies for the clinical management of afflicted patients. Numerous cardiovascular disorders are charact...
Article
The recrudescence of syphilis is leading to the development of new serological tests. The goal of this study was to compare the performance of the more recent Elecsys Syphilis assay, the Electro Chemiluminescence Immunoassay (ECLIA), with the former Architect Syphilis TP assay, the Chemiluminescent Microparticle Immunoassay (CMIA), for the detectio...
Article
Full-text available
Human serum contains a physiological plethora of bioactive elements naturally released by activated platelets which might have a significant effect on the regeneration of corneal layers by stimulating the cell growth. This mechanism supported the use of human serum eye drops in some ocular diseases associated with dystrophic changes and alterations...
Article
Full-text available
Cardiovascular disease is still the major cause of death worldwide despite the remarkable progress in its prevention and treatment. Endothelial progenitor cells have recently emerged as key players of vascular repair and regenerative medicine applied to cardiovascular disease. A large amount of effort has been put into discovering the factors that...
Article
Full-text available
Serological assays are still considered the most useful tests in the diagnosis of syphilis. Since no single serological assay is able to provide a satisfactory result, in our laboratory we have evaluated the usefulness of a commercially immunoblot to diagnose syphilis infection among blood donors. From October 2012 to June 2013, 4572 blood donors w...
Article
Full-text available
Bartonella henselae is the etiologic agent of cat-scratch disease. B. henselae infections are responsible for a widening spectrum of human diseases, although often symptomless, ranging from self-limited to life-threatening and show different courses and organ involvement due to the balance between host and pathogen. The role of the host immune resp...
Article
Full-text available
Recent efforts in transfusion medicine are focused on improving blood safety as well as establishing effective and efficient diagnostic algorithms for donor screening. To date, syphilis is a transfusion-transmitted infection re-emerged in many countries as a public health threat especially among populations at specific risk. This task requires new...
Article
Prior preclinical and clinical studies support the use of platelet-derived products for the treatment of soft and hard tissue lesions. These regenerative effects are controlled by autocrine and paracrine biomolecules including growth factors and cytokines contained in platelet alpha granules. Each growth factor is involved in a phase of the healing...
Article
From the 1980s, extracorporeal photochemotherapy (ECP) has been shown to be effective in a variety of pathological conditions such as erythrodermic cutaneous T-cell lymphoma, autoimmune diseases, solid organ allograft rejection and graft versus host disease. To date, ECP represents a non-aggressive immune modulatory therapy with a low spectrum of t...
Article
Recently, management of patients awaiting solid organ transplantation has taken advantages after the development of more sensitive and accurate solid phase assays which have supported the historic complement dependent cytotoxicity. This approach has allowed the detection of antibodies in patients previously considered negative. The use of the singl...
Article
Full-text available
Automated chemiluminescent immunoassays (CLIAs) are useful for the detection of hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus 1/2 antigen/antibodies (HIV 1/2 Ag/Ab) in blood donor screening. Eight hundred and forty serum samples were tested for hepatitis B surface antigen (HBsAg), HCV antibodies (anti-HCV), and...
Article
Full-text available
Screening assays are needed in order to guarantee safety of donated blood, but a significant number of safe donations are removed from blood supply because of reactive screening results. It is important to evaluate the positive predictive value (PPV) of screening assays in order to modulate confirmatory algorithm and implement an adequate counselin...
Article
Full-text available
Mediator complex (MED) is an evolutionarily conserved multiprotein, fundamental for growth and survival of all cells. In eukaryotes, the mRNAs transcription is dependent on RNA polymerase II that is associated to various molecules like general transcription factors, MED subunits and chromatin regulators. To date, transcriptional machinery dysfuncti...
Article
Aim Previous clinical studies indicate that implantation of Bone Marrow Cells (BMCs) into ischemic limbs may improve peripheral ischemia. We previously demonstrated that intraarterial administration of autologous BMC and antioxidants and L-arginine therapy is safe and effective in patients with advanced atherosclerotic peripheral artery disease (PA...
Article
Aim Recent evidences have shown that several host genetic factors influence susceptibility or protection to hepatitis C virus (HCV) infection. There are controversial data regarding the associations of human leukocyte antigens (HLA) and clearance or progression of HCV. The aim of this study was to investigate whether particular HLA molecules were a...
Article
Aim The twin epidemics of obesity and Type 2 diabetes continue to increase in industrialized nations. Approximately two thirds of adult Americans and Western Countries are currently overweight or obese and therefore at increased risk for a number of deleterious health conditions including Type 2 diabetes and Cardiovascular Disease (CVD). Consumptio...
Article
Recent evidences have shown that several host genetic factors influence susceptibility or protection to hepatitis C virus (HCV) infection. There are controversial data regarding the associations of human leukocyte antigens (HLA) and the clearance or progression of HCV. The aim of this study was to investigate whether particular HLA molecules were a...
Article
Full-text available
Nitric oxide (NO) has been suggested to be a pathophysiological modulator of cell proliferation, cell cycle arrest and apoptosis. In this context, opposite effects can be exerted by NO under diverse conditions. Indeed, several studies have indicated that low relative concentrations of NO seem to favour cell proliferation and anti-apoptotic response...
Article
Full-text available
The use of cardiac resynchronization therapy (CRT) and implantable cardioverter- defibrillator (ICD) for advanced heart failure (HF) is increasing. Renal dysfunction is a common condition in HF which is associated with a worse survival. The study aims at identifying in patients with advanced HF treated with CRT the effect of baseline glomerular fil...
Article
Full-text available
Bartonella henselae is able to internalize endothelial progenitor cells (EPCs), which are resistant to the infection of other common pathogens. Bacteroides fragilis is a gram-negative anaerobe belonging to the gut microflora. It protects from experimental colitis induced by Helicobacter hepaticus through the polysaccharide A (PSA). The aim of our s...
Data
Bacteroides EPC internalization at different MOIs. Confocal images of human early EPCs infected with B. fragilis and B. fragilis ΔPSA at 100, 200 and 400 MOI. Cells were stained with anti-Bacteroides (red) specific antibody after 48 h from infection. (TIF)
Data
Differential expression of inflammatory genes in infected EPCs. Percentage of differentially expressed inflammatory genes after EPC infection with B. henselae, B. fragilis, B. fragilis ΔPSA, B. henselae and B. fragilis, B. henselae and B. fragilis ΔPSA respectively, compared to uninfected EPCs. (TIF)
Data
Bacteroides and Bartonella EPC coinfection. EPCs coinfected with B. henselae, and B. fragilis or B. fragilis ΔPSA as indicated. A MOI of 100 was used for all bacteria strains. Cells were stained with anti-Bacteroides (red) and with anti-Bartonella (green) specific antibodies after 24 h, 48 h and 72 h from infection. (TIF)
Data
Levels of secreted inflammatory cytokines in infected EPCs measured by ELISA. Bar graph showing the ELISA results from an array of 12 secreted cytokines involved in the inflammation response. (TIF)
Data
Morphological analysis of murine infected spleen by hematoxylin-eosin and immunohistochemistry. A. Representative microscope images of hematoxylin-eosin staining of spleen tissues from each group of mice uninfected, infected with B. henselae, B. fragilis and B. fragilis ΔPSA or coinfected, as detailed. B. Immunohistochemistry analysis of murine spl...
Data
Oligonucleotides used in this study. (DOC)
Data
Electron microscopy analysis of liver (panels A–E) and aorta (panels F–L) from infected mice. A. B. fragilis (arrow). AI. Detail of panel A. B. B. fragilis ΔPSA (arrow). BI. Sinusoidal Kupffer cell with evident phagolysosome. C. B. henselae (arrow) in a granuloma with a characteristic neutrophil. CI. B. henselae in the endothelial layer. D. Bacteri...
Conference Paper
Bartonella henselae è un batterio Gram-negativo intracellulare facoltativo responsabile della malattia da graffio di gatto (cat-scratch disease), un'infezione caratterizzata da linfoadenopatia o da un decorso asintomatico in pazienti immunocompetenti. Tuttavia, l'angiomatosi bacillare e la peliosi epatica, nonchè l'endocardite in pazienti con malat...
Article
Full-text available
The relevance of anti-HLA antibodies in patients awaiting kidney transplants is well recognized. During the past 40 years, kidney transplant candidates have been tested for these antibodies, and the choice of the detection assay has become essential. Recently, the pioneer method, the complement-dependent cytotoxicity, has been integrated but has no...
Data
Summary of mapping results. Distribution of the sequenced reads according to the mapping procedure. DS sample (A) and Euploid (B). (JPG)
Data
Distribution of the UARs in the human genome. Distribution of the UARs according to RefSeq genes, intronic intergenic regions and mitochondrial chromosome. DS sample (A) and Euploid (B). (JPG)
Data
Differential expression of igTARs and inTARs. Standard MA-plot of the normalized global observed counts per each identified igTAR (A) and inTAR (B). Venn diagrams showing the number of regions with evidence of DE according to each statistical method used (igTARs in panel C and inTARs in panel D). (JPG)
Data
Experimental procedure. Schematic representation of the whole RNA-Seq experiment. Depicted are: Total RNA isolation (1) and ribo-depletion (2). Ribo-depleted total RNA is fragmented (3), then ligated to specific adaptors (4) and retro-transcribed (5). The resulting cDNA is size selected by gel electrophoresis (6), and cDNAs are PCR amplified (7). T...
Data
Detection of alternative splicing events. Schematic representation of the computational analysis used to detect sample-specific ASEs both canonical and unannotated. Reliability of the junction was measured with T1 = 3 (A) and with T1 = 5 (B). (JPG)
Data
Supporting Materials and Methods. (DOC)
Data
Differential expression of lincRNAs. Standard MA-plot of the normalized global observed counts per each lincRNA. (JPG)
Data
Data analysis pipeline. Schematic representation of the data analysis workflow described in detail in “Materials and Methods”. (JPG)
Data
Quantitative Real-Time PCR validation. A random selection of “no change” (A) and weak DE (B) RefSeq genes between the analyzed samples confirmed by qRT-PCR. Relative expression levels for a selection of DE RefSeq genes in DS state (C). (JPG)
Data
Summary of mapping on the junctions. (DOC)
Data
Distribution of RPKM expression level of snoRNA host genes. (DOC)
Data
List of differentially expressed snoRNAs in human trisomy 21. (DOC)
Data
Primer pairs used for quantitative RT-PCR. (DOC)
Article
Full-text available
Hybridization- and tag-based technologies have been successfully used in Down syndrome to identify genes involved in various aspects of the pathogenesis. However, these technologies suffer from several limits and drawbacks and, to date, information about rare, even though relevant, RNA species such as long and small non-coding RNAs, is completely m...
Article
Full-text available
Pathological angiogenesis represents a critical issue in the progression of many diseases. Down syndrome is postulated to be a systemic anti-angiogenesis disease model, possibly due to increased expression of anti-angiogenic regulators on chromosome 21. The aim of our study was to elucidate some features of circulating endothelial progenitor cells...