Lina Yonekura

• PhD
• Professor at Kagawa University

Guaraná (Paullinia cupana) seeds are rich in flavan-3-ols (catechins) and reduce oxidative stress in humans. We determined the concentration and in vitro bioaccessibility of catechins in guaraná seeds from two geographical origins, and measured their Caco-2 permeability. The effect of macronutrients on catechins' bioaccessibility and Caco-2 permeab...

Olive ( Olea europaea ) phytochemicals are associated with a reduced risk of type 2 diabetes. Table olives typically have higher phytochemical concentrations than olive oil, but their impact on human intestinal α-glucosidase is largely unknown. Shinzuke, the most common trade preparation in Japan, are alkali-treated non-fermented green olives. In c...

The assessment of carotenoid bioavailability has long been hampered by the limited knowledge of their absorption mechanisms. However, recent reports have elucidated important aspects of carotenoid digestion and absorption. Disruption of food matrix and increasing amounts of fat seem to enhance the absorption of carotenes to a larger extent than tha...

The antiallergic activity of extracts of Naruto Kintoki sweet potato peel was enhanced after cooking the peel using three conventional methods. Using 400 µg/mL extracts, baking showed the highest suppression of β-hexosaminidase release (36.9 %), followed by microwaving (41.9 %) and boiling (64.2 %). Significant suppression of β-hexosaminidase relea...

The objective of this study was to determine which volatile compounds are responsible for the aroma of fresh sweet cream butter, using a liquid–liquid extraction method coupled with a Porapak Q column and limited odour units (Lod) as technical methods. Sixty-four compounds were quantitatively identified. Assessors determined 100 ppm as the critical...

Most methods for quantification of catechins in guaraná and other food matrices rely on lengthy gradients to resolve all peaks, and the analysis time for one sample is around 45-135 min. The present method is a fast, sensitive and simple HPLC-UV method, with a 16-minute isocratic run that deliver the high throughput needed to process a large number...

Rosmarinic acid (RA), commonly found in Nepetoidae subfamily of Lamiaceae family, possesses various biological activities. To expand its application, RA was modified by esterification with methyl (me), propyl (pro), and hexyl (hex) alcohols and then tested antibacterial, α‐glucosidase inhibitory, and lipid accumulation suppression activities. Conse...

Cytotoxicity on sonchifolinic acid (1) isolated from Samallunthus leaf and synthetic compounds related to compound 1 were comparatively studied for the first time. Derivatives of 1 such as propyl (1a), butyl (1b), pentyl (1c) and 2-methoxy ethyl (1d) sonchifolate were prepared and their cytotoxic activity on HeLa cells were compared with sonchifoli...

In the present paper ionotropically structured κ-carrageenan based oil-in-gel (o/g) emulsions were tested as potential carrier systems for the delivery of β-carotene. In situ ionic gelation was induced by Na+, K+ or Ca2+ added at the level of 0.2-0.6% (w/w). All o/g emulsions exerted a true gel like behaviour with storage modulus (G´) being reduced...

In the present work oil-in-gel (o/g) emulsions comprising 0.4 to 1% w/w κ-carrageenan in the absence and presence of Ca²⁺ (20 mM) were investigated for their ability to hamper the oxidative degradation under isothermal (5, 20 and 37 °C) ambient air storage conditions and promote the in vitro bioaccessibility of β-carotene. The mechanical and struct...

We assessed the effects of guaraná (Paullinia cupana) consumption on plasma catechins, erythrocyte antioxidant enzyme activity (superoxide dismutase, catalase and glutathione peroxidase) and biomarkers of oxidative stress (ex vivo LDL oxidation, plasma total antioxidant status and ORAC, and lymphocyte single cell gel electrophoresis) in healthy ove...

Objective: The objectives of this study were to investigate the antioxidant activities in peel and seed of six tropical fruits from Indonesia and to evaluate their bioavailability by in vitro digestion model. Methods: Six tropical fruits namely kapundung, matoa, papaya, rambai salak and soursop were used. The study measured antioxidant activities t...

Absorption, metabolism, and excretion of 3,4-DHPEA-EDA, oleuropein, and hydroxytyrosol isolated from olive fruits were newly evaluated after oral and intravenous administration in freely moving rats cannulated in the portal vein, jugular vein, and bile duct. Orally administered 3,4-DHPEA-EDA, an important bioactive compound in olive pomace, was rea...

To improve the intestinal absorption of fucoxanthin, we evaluated the effects of dietary glyceroglycolipids on the uptake and secretion of fucoxanthin solubilized in mixed micelles by human intestinal Caco-2 cells. Although digalactosyldiacylglycerol and sulfoquinovosyldiacylglycerol suppressed fucoxanthin uptake and secretion, their lyso-types, di...

We previously reported that phospholipids markedly affected the uptake of carotenoids solubilized in mixed micelles by human intestinal Caco-2 cells. In the present study, we found that two classes of dietary glyceroglycolipids and the corresponding lysoglyceroglycolipids affected uptake of β-carotene and lutein by differentiated Caco-2 cells. The...

Methyl, propyl and hexyl esters of rosmarinic, caffeic and p-coumaric acids were tested for antiallergic activity, and rosmarinic acid propyl ester exhibited the greatest β-hexosaminidase release suppression (IC50, 23.7 μM). Quadratic correlations between pIC50 and cLogP (r2 = 0.94, 0.98, and 1.00, respectively) were observed in each acid ester ser...

Forty-six kinds of vegetables and fruits were tested the antiallergic activity by means of suppression rates of a histamine related substance-release from RBL-2H3 cells. Solid Phase Extraction Technique with QuEChERS (STQ) method was applied for quick and efficient extraction of active food ingredients. Consequently we found that sweet potato, lotu...

In the present paper, a novel approach for the development of probiotic baked cereal products is presented. Probiotic pan bread constructed by the application of film forming solutions based either on individual hydrogels e.g. 1% w/w sodium alginate (ALG) or binary blends of 0.5% w/w sodium alginate and 2% whey protein concentrate (ALG/WPC) contain...

The concept of prebiotic edible films as effective vehicles for encapsulating probiotic living cells is presented. Four soluble fibres (inulin, polydextrose, glucose- oligosaccharides and wheat dextrin) were selected as prebiotic co-components of gelatine based matrices plasticised with glycerol and used for the immobilisation of L. rhamnosus GG. T...

We evaluated sodium alginate, chitosan and hydroxypropyl methylcellulose (HPMC) as co-encapsulants for spray dried Lactobacillus acidophilus NCIMB 701748 by assessing their impact on cell viability and physicochemical properties of the dried powders, viability along 35 days of storage at 25°C and survival after simulated digestion. Fibres were adde...

Supplementary Data:

Lipids are minor components of flours, but are major determinants of baking properties and end-product quality. To the best of our knowledge there is no single solvent system currently known that efficiently extracts all non-starch lipids from all flours without the risk of chemical, mechanical or thermal damage. This paper compares nine ambient so...

Over recent years the use of spray drying for the production of anhydrobiotics has gained the interest of functional food manufacturers, mainly due cost efficiencies and enhanced product and process flexibility (e.g. enhanced shelf life). In the present work, spray drying conditions (air inlet temperature and feed flow rate) were optimized for the...

We developed and validated a method for the extraction and determination of eleven phenolic acids in rat plasma, urine and liver by ultra-performance liquid chromatography-mass spectrometry (UPLC-MS). System Suitability Test (instrumental linearity, area and retention time precision) was performed and recovery, intra and between-day precision, dete...

Three different milk proteins — skim milk powder (SMP), sodium caseinate (SC) and whey protein concentrate (WPC) — were tested for their ability to stabilize microencapsulated L. acidophilus produced using spray drying. Maltodextrin (MD) was used as the primary wall material in all samples, milk protein as the secondary wall material (7:3 MD/milk p...

Para elaboração de uma fonte semi-convencional de proteínas de peixes pouco comercializáveis, foram utilizados filés de Aruanã (Osteoglossum bicirrhosum Vandelli, 1899, Osteoglossidae) adquiridos recém capturados no porto do Educandos (Manaus, Amazonas) e transportados para o laboratório de Tecnologia de Alimentos do INPA onde foram triturados e ho...

Resumo: Para elaboração de uma fonte semi-convencional de proteínas de peixes pouco comercializáveis, foram utilizados lés de Aruanã (Osteoglossum bicirrhosum Vandelli, 1899,) adquiridos recém capturados no porto do Educandos (Manaus, Osteoglossidae Amazonas) e transportados para o laboratório de Tecnologia de Alimentos do INPA onde foram triturad...

Fucoxanthin, a xanthophyll present in brown algae consumed in Eastern Asia, can suppress carcinogenesis and obesity in rodents. We investigated the metabolism, tissue distribution, and depletion of fucoxanthin in ICR mice by comparison with those of lutein. The experiments comprised 14-d dietary supplementation with lutein esters or fucoxanthin, fo...

The effects were evaluated of various glycerophospholipids on the uptake of beta-carotene solubilized in mixed micelles by human intestinal Caco-2 cells. Phosphatidylethanolamine markedly enhanced the transfer of beta-carotene from the micelles to the cells, whereas phosphatidylcholine suppressed it. All the lysoglycerophospholipids enhanced the tr...

We evaluated the effects of soluble fibers on beta-carotene and lutein micellization during simulated digestion in vitro, and on carotenoid uptake from mixed micelles by Caco-2 cells. Medium- and high-viscosity alginates and pectins inhibited carotenoid micellization and cellular uptake relative to the fiber-free control. Alginates, carboxy-methylc...

Epoxyxanthophylls (epoxide-containing xanthophylls), a group of carotenoids, are ubiquitously distributed in edible plants. Among them, neoxanthin in green leafy vegetables and fucoxanthin in brown algae have been reported to exhibit an antiproliferative effect on several human cancer cells in vitro. However, there is little information about the i...

The intestinal absorption of carotenoids is thought to be mediated by the carotenoid assembly in mixed micelles, followed by its transfer into the enterocytes and subsequent secretion to the lymph as chylomicron particles. In the present study we investigated the effects of phospholipids and lysophospholipids with diverse fatty acyl moieties on the...

Owing to its fermentability, it has been advocated that resistant starch (RS) has a positive effect on the absorption of minerals by increasing their solubility in the hindgut. In marginally zinc-deficient rats, the enhancement of zinc bioavailability by RS occurs mostly when the diet contains phytic acid. This study aims to investigate the effect...

Many members of the Anthemideae (Asteraceae) have deeply-entrenched cultural roots and are important as cut ornamental flowers and as aromatic and medicinal plants, many producing secondary metabolites used in folk and modern medicine, the cosmetic and pharmaceutical industries. These compounds produced by various species within this tribe will con...

The contribution of cecal fermentation of polysaccharides to the restoration of zinc bioavailability suppressed by phytic acid was assessed through the simultaneous evaluation of changes in zinc and magnesium absorption. Three weeks old rats were fed Fiber-free, 1% Chitosan, 2% Chitosan, 7% RS (raw potato starch), 14% RS, 2.5% Alginic acid or 5% Al...

With the objective of restoring the low zinc bioavailability of phytate-containing diets, cellulose was replaced by pectin, alginic acid, carrageenan, chitosan or raw potato starch (RS), in phytate-free and phytate-containing diets, and given to rats for 21 days. Feeding chitosan, alginic acid or RS (200 g/kg) as the dietary fiber polysaccharide le...

The chemical composition of the duplicate food collections obtained from 36 servers of the National Institute of Researches of Amazônia (INPA) was evaluated and as percentage of recommended intake. The most frequently food consumed they were: sugar (91.7%), rice (80.6%), coffee (77.8%), milk (72.3%), bovine meat and bread (63.9%), cassava flour (58...

Composition centesimal and minerals elements were determined in banana pacovã (Musa paradisiaca): ripe (in natura, boiled and fried), fresh (in natura and fried). Samples were processed in the Laboratório de Nutrição e Físico-Química de Alimentos da Coordenação de Pesquisas em Ciência da Saúde - INPA. These results showed banana pacovã good source...

It was determined the nutritional constituents of the pre-school children diets from Nhamundá - AM, followed by the nutrient adequacy ratio. For sampling, the duplicate portion technique was used, which consisted of collecting all the foods and beverages consumed during 1 day. The results shown inadequate caloric contents, besides presenting defici...

A study was conducted to determine the nutrient content and nutrient adequacy ratio of the foods offered to preschool children at the Fundacao Hospitais e Creches do Amazonas (Amazonas, Brazil) during May-June 1997. Food samples (breakfast, lunch and snacks) were collected in duplicate and were chemically analysed. The most frequently consumed food...

Considerando a magnitude da hipovitaminose A como problema de saúde pública no mundo e a disponibilidade de frutos ricos em pró-vitamina A, como a pupunha (Bactris gasipaes Kunth) na região Amazônica, determinou-se a biodisponibilidade de vitamina A da mesma em ratos, utilizando o método preventivo. Os resultados indicaram ser a pupunha uma fonte d...

Considerando a magnitude da hipovitaminose A como problema de saúde pública no mundo e a disponibilidade de frutos ricos em pró-vitamina A na região Amazônica, determinou-se a biodisponibilidade dos carotenóides do Buriti (Mauritia flexuosa L.) em ratos. Quarenta e oito ratos machos da linhagem Wistar (Rattus novergicus, var. albinus, Rodentia: Mam...

Vitamin A deficiency is a serious public health problem in the world. In Brazilian Amazonia, some native fruits may be pointed as good sources of this vitamin, provided their abundant pro-vitamin A present high bioavailability. The present study evaluated the bioavailability of carotenoids from Buriti (Mauritia flexuosa L.) in rats. Forty eight rec...