Lim Chang Siang

Lim Chang Siang
National University of Singapore | NUS · Saw Swee Hock School of Public Health

BSc(Hons)BioMed, GDipSA

About

18
Publications
1,839
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156
Citations

Publications

Publications (18)
Article
Full-text available
The prevalence of gestational diabetes mellitus (GDM) is increasing, and only a few mobile health (mHealth) applications are specifically designed to manage GDM. In this mixed-methods study, a follow-up study of a randomized controlled trial (RCT) analyzed a largely automated mHealth application-based lifestyle coaching program to (a) measure the a...
Article
Full-text available
Heterozygous HNF1A gene mutations can cause maturity onset diabetes of the young 3 (MODY3), characterized by insulin secretion defects. However, specific mechanisms of MODY3 in humans remain unclear due to lack of access to diseased human pancreatic cells. Here, we utilize MODY3 patient-derived human induced pluripotent stem cells (hiPSCs) to study...
Article
Full-text available
Human embryonic stem cells are a type of pluripotent stem cells (hPSCs) that are used to investigate their differentiation into diverse mature cell types for molecular studies. The mechanisms underlying insulin receptor (IR)-mediated signaling in the maintenance of human pluripotent stem cell (hPSC) identity and cell fate specification are not full...
Conference Paper
Full-text available
We present a case study where we developed an interface for remote vital signs self-monitoring at a large-scale isolation facility for COVID-positive patients, under disrupted conditions. These conditions were: a lack of time, lack of access to end users, changing requirements, high risk of infection and supply chain limitations. We first describe...
Article
Full-text available
Objective: SMART-GDM examined whether Habits-GDM, a smartphone application (app) coaching program, can prevent excessive gestational weight gain (EGWG) and improve glycemic control and maternal and neonatal outcomes in gestational diabetes mellitus (GDM). Research design and methods: In this randomized controlled trial, women diagnosed with GDM be...
Article
Full-text available
Although IKK-β has previously been shown as a negative regulator of IL-1β secretion in mice, this role has not been demonstrated in humans. Genetic studies of NF-κB signalling in humans with inherited diseases of the immune system have not demonstrated the relevance of the NF-κB pathway in suppression of IL-1β expression. Here, we report an infant...
Preprint
BACKGROUND The prevalence of gestational diabetes mellitus (GDM) is increasing in Singapore and the cost of providing traditional care for GDM is high. Mobile health (mHealth) applications may act as useful tools in the management of GDM. OBJECTIVE The objective of this mixed-methods study was to measure the usage behaviour and explore users’ perc...
Article
Pancreatic β cells are responsible for insulin secretion and are important for glucose regulation in a healthy body and diabetic disease patient without prelabeling of islets. While the conventional biomarkers for diabetes have been glucose and insulin concentrations in the blood, the direct determination of the pancreatic β cell mass would provide...
Article
Full-text available
Maturity-onset diabetes of the young 1 (MODY1) is a monogenic diabetes condition caused by heterozygous HNF4A mutations. We investigate how HNF4A haploinsufficiency from a MODY1/HNF4A mutation influences the development of foregut-derived liver and pancreatic cells through differentiation of human induced pluripotent stem cells from a MODY1 family...
Article
Full-text available
Human pancreatic islets containing insulin-secreting β-cells are notoriously heterogeneous in cell composition. Since β-cell failure is the root cause of diabetes, understanding this heterogeneity is of paramount importance. Recent reports have cataloged human islet transcriptome but not compared single β-cells in detail. Here, we scrutinized ex vi...
Article
The cover image, by Larry Sai Weng Loo et al., is based on the Review Article An arduous journey from human pluripotent stem cells to functional pancreatic β cells, DOI: 10.1111/dom.12996.
Article
Full-text available
Type 1 and type 2 diabetes are caused by either a destruction or decrease in the number of functional insulin-producing β-cells, respectively. Therefore, the generation of functional β-cells from human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs), collectively known as human pluripotent stem cells (hPSCs), for pote...

Questions

Question (1)
Question
Hi. I have recently purchase the alpha-TC9 rodent glucagonoma from ATCC. According to ATCC protocol, the media should contain 0.02% BSA. Can anyone help to recommend a suitable brand for the BSA to culture this cell line? Thank you!

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