Lilian Kisiswa

Lilian Kisiswa
Karolinska Institutet | KI · Department of Cell and Molecular Biology

PhD

About

41
Publications
4,079
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406
Citations
Additional affiliations
August 2013 - June 2017
Karolinska Institutet
Position
  • PostDoc Position
March 2011 - August 2013
School of Biosciences
Position
  • PostDoc Position
March 2011 - July 2013
Cardiff University
Position
  • PostDoc Position

Publications

Publications (41)
Preprint
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Background Cytosine arabinoside (AraC) is one of the main therapeutic treatments for several types of cancer including acute myeloid leukaemia. However, after high dose AraC chemotherapy regime, patients develop severe neurotoxicity and neurodegeneration in the central nervous system leading to cerebellar ataxia, dysarthria, nystagmus, somnolence a...
Preprint
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Background Huntington’s disease (HD) is a fatal neurodegenerative disorder characterized by progressive motor dysfunction and loss of medium spiny neurons (MSNs) in dorsal striatum. Brain-derived neurotrophic factor (BDNF) sustains functionality and integrity of MSNs, and thus reduced BDNF signaling is integral to the disease. Mutations in BDNF rec...
Preprint
Full-text available
Background: Huntington’s disease (HD) is a fatal neurodegenerative disorder characterized by progressive motor dysfunction and loss of medium spiny neurons (MSNs) in dorsal striatum. Brain-derived neurotrophic factor (BDNF) sustains functionality and integrity of MSNs, and thus reduced BDNF signaling is integral to the disease. Mutations in BDNF re...
Preprint
Full-text available
The medial habenula (mHb) is an understudied small brain nucleus linking forebrain and midbrain structures controlling anxiety and fear behaviors. The mechanisms that maintain the structural and functional integrity of mHb neurons and their synapses remain unknown. Using spatio-temporally controlled Cre-mediated recombination in adult mice, we foun...
Article
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The p75 neurotrophin receptor (p75NTR) is a critical mediator of neuronal death and tissue remodeling and has been implicated in various neurodegenerative diseases and cancers. The death domain (DD) of p75NTR is an intracellular signaling hub and has been shown to interact with diverse adaptor proteins. In breast cancer cells, binding of the adapto...
Preprint
p75 neurotrophin receptor (p75NTR) is a critical mediator of neuronal death and tissue remodeling and has been implicated in various neurodegenerative diseases. The death domain (DD) of p75NTR is an intracellular signaling hub and has been shown to interact with diverse adaptor proteins. However, the structural mechanism and physiological relevance...
Article
Full-text available
The plasticity mechanisms in the nervous system that are important for learning and memory are greatly impacted during aging. Notably, hippocampal‐dependent long‐term plasticity and its associative plasticity, such as synaptic tagging and capture (STC), show considerable age‐related decline. The p75 neurotrophin receptor (p75NTR) is a negative regu...
Article
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An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Article
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MAG (Myelin-associated glycoprotein) is a type I transmembrane glycoprotein expressed by Schwann cells and oligodendrocytes, that has been implicated in the control of axonal growth in many neuronal populations including cerebellar granule neurons (CGNs). However, it is unclear whether MAG has other functions in central nervous system, in particula...
Article
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Members of the TNF and TNF receptor superfamilies acting by both forward and reverse signaling are increasingly recognized as major physiological regulators of axon growth and tissue innervation in development. Studies of the experimentally tractable superior cervical ganglion (SCG) neurons and their targets have shown that only TNF reverse signali...
Article
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Cerebellar granule neurons (CGNs) undergo programmed cell death during the first postnatal week of mouse development, coincident with sustained expression of the death receptor p75NTR. Although ablation of p75NTR does not affect CGN cell death, deletion of the downstream effector RIP2 significantly increases CGN apoptosis, resulting in reduced adul...
Article
Full-text available
Tumour necrosis factor receptor 1 (TNFR1)-activated TNFα reverse signalling, in which membrane-integrated TNFα functions as a receptor for TNFR1, enhances axon growth from developing sympathetic neurons and plays a crucial role in establishing sympathetic innervation. Here, we have investigated the link between TNFα reverse signalling and axon grow...
Article
Full-text available
Signaling by the p75 neurotrophin receptor (p75(NTR)) is often referred to as cell-context dependent, but neuron-type specific signaling by p75(NTR) has not been systematically investigated. Here, we report that p75(NTR) signals very differently in hippocampal neurons (HCNs) and cerebellar granule neurons (CGNs), and present evidence indicating tha...
Article
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APRIL (A Proliferation-Inducing Ligand, TNFSF13) is a member of the tumor necrosis factor superfamily that regulates lymphocyte survival and activation and has been implicated in tumorigenesis and autoimmune diseases. Here we report the expression and first known activity of APRIL in the nervous system. APRIL and one of its receptors, BCMA (B-Cell...
Article
Full-text available
Dendrite size and morphology are key determinants of the functional properties of neurons. Here, we show that growth differentiation factor 5 (GDF5), a member of the bone morphogenetic protein (BMP) subclass of the transforming growth factor β superfamily with a well-characterised role in limb morphogenesis, is a key regulator of the growth and ela...
Article
Full-text available
Reverse signaling via members of the tumor necrosis factor (TNF) superfamily controls multiple aspects of immune function. Here we document TNFα reverse signaling in the nervous system to our knowledge for the first time and show that it has a crucial role in establishing sympathetic innervation. During postnatal development, sympathetic axons expr...
Article
Full-text available
RANKL (receptor-activator of NF-κB ligand, TNFSF11) is a member of the TNF superfamily that regulates bone remodelling and the development of the thymus, lymph nodes and mammary glands. While RANKL and its membrane bound receptor RANK (TNFRSF11A) are expressed in the adult central nervous system and have been implicated in thermoregulation, the pot...
Article
Apoptosis, is the main type of cell death that occurs in ageing and neurodegenerative disease, such as glaucoma. This study therefore characterises the expression profile of caspases (pro-apoptosis) and inhibitors of apoptosis (IAPs; anti-apoptosis) during maturation of the Brown Norway rat retina between 6 weeks and >24 weeks and also examines con...
Article
The development of a method for the sustained elevation of intraocular pressure in experimental glaucoma based on the anterior chamber injection of paramagnetic microbeads. Unilateral glaucoma was induced in adult male Norwegian Brown rats by the injection of paramagnetic polystyrene microspheres. A handheld 0.45 Tesla magnet was used to draw the b...
Article
Glaucoma is characterised by the preferential death of retinal ganglion cells (RGCs). However, mammalian models indicate that neurons pass through a period in which they manifest signs of neuronal damage, but have yet to fully commit to death. Mounting evidence suggests that one of the clearest indications of this process is the reduction in RGC de...
Article
Full-text available
Evidence is emerging for apoptosis gene expression in the lens during development. Therefore, here we used a filter array to assess expression of 243 apoptosis-related genes in the developing postnatal mouse lens using (33)P labelled cDNA synthesized from p7 and p14 mouse lenses. We demonstrated that 161 apoptosis-related genes were expressed at le...
Article
Full-text available
Magnetic resonance imaging (MRI) is a powerful tool for generating 3-dimensional structural and functional image data. MRI has already proven valuable in creating atlases of mouse and quail development. Here, we have exploited high resolution MRI to determine the parameters necessary to acquire images of the chick embryo eye. Using a 9.4 Tesla (400...
Data
Phosphoimage of an array hybridised with P7 radiolabelled RNA. Examples of positive spots are provided, including genomic DNA spots for orientation of the array as well as housekeeping genes and apoptosis genes (caspase-7 and 14-3-3 eta).
Data
Reproducibility of array results. Scatter plots (A–E) were produced by plotting the normalised signal intensities from one array against another. Array results were compared between arrays that had been treated identically (i.e. fresh array compared with a fresh array, or a stripped array compared with a stripped array) or that had been treated dif...
Data
Graphical representations of representative Western blots using densitometry normalised against the actin control at each stage examined for chick Mdm2, Mdm4/X and p53. Error bars represent standard deviation of the mean for a minimum of 3 replicates in each case. (A) The Mdm2-positive band at 55 kDa was faint at embryonic day (ED)6-ED8, peaked in...
Data
RT-PCRs reveal expression of Mdm2, Mdm4/X and p53 during embryonic stages of mouse lens development. PCRs were also carried out on cDNA prepared as described in Materials and Methods from E12.5 pooled mouse embryo whole eyes and on pooled lenses from E14.5, E16.5 as well as the post-natal stages examined previously. All three genes were differentia...
Data
Graphical representations of representative Western blots using densitometry normalised against the actin control at each stage examined for mouse Mdm2, Mdm4/X and p53. Error bars represent standard deviation of the mean for a minimum of 3 replicates in each case. (A) Mdm2 expression at approximately 55 kDa was detected in the Nb lens, peaked at p7...
Data
Standard curves for semi-quantitative PCR of chicken embryo lens. Standard curves were completed for three genes expressed at relatively high (GAPDH), intermediate (13-3-3 eta) and low levels (Mdm2) to determine the number of cycles to be used to ensure the PCR was in the linear phase of the reaction. The increase in band intensity with increasing...
Data
Graphical representations of representative Western blots using densitometry normalised against the actin control at each stage examined for mouse Axl-1 and Mcl-1. Error bars represent standard deviation of the mean for a minimum of 3 replicates in each case. (A) Axl expression peaked at Nb and had a constant low level of expression thereafter to 4...
Article
Full-text available
Multiple sclerosis (MS) is a chronic, progressive disease of the central nervous system (CNS) characterized by consistent myelin injury. Antibody-mediated death of oligodendrocytes is a pathological feature in a subset of MS patients and may be of relevance to disease pathogenesis. In myasthenia gravis (MG), acetylcholine receptors (AChR) situated...

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