Lilian Castilho

Lilian Castilho
University of Campinas | UNICAMP · Centro de Hematologia e Hemoterapia (HEMOCENTRO)

PhD

About

200
Publications
39,880
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2,359
Citations
Citations since 2016
75 Research Items
1247 Citations
2016201720182019202020212022050100150200
2016201720182019202020212022050100150200
2016201720182019202020212022050100150200
2016201720182019202020212022050100150200

Publications

Publications (200)
Article
Introdução e relato de caso Um sangue raro é definido como negativo para antígeno de alta prevalência, com frequência de 1:1000 ou menos ou negativo para vários antígenos comuns. Alguns fenótipos raros são restritos a determinadas áreas geográficas do mundo e indivíduos. Neste relato, reportamos a identificação do raro fenótipo ryry k-negativo em u...
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Introdução Atualmente, mais de 400 tipos de antígenos D fracos foram identificados molecularmente. Os tipos mais comuns na Europa e América do Norte são os tipos 1, 2 e 3. No Brasil, embora esses tipos D fracos sejam menos frequentes, ainda são considerados os mais comuns, seguidos por D fraco tipo 4. Outros tipos de D fracos são raros e encontrado...
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Introdução e relato de caso O sistema de grupos sanguíneos Kell consiste em 36 antígenos considerados clinicamente relevantes carreados em uma glicoproteína transmembrana tipo II. Anticorpos do sistema Kell podem causar reações transfusionais graves, bem como doença hemolítica do feto e do recém-nascido. Neste estudo, relatamos um novo alelo KEL*02...
Article
Background and objectives: Under the ISBT, the Working Party (WP) for Red Cell Immunogenetics and Blood Group Terminology is charged with ratifying blood group systems, antigens and alleles. This report presents the outcomes from four WP business meetings, one located in Basel in 2019 and three held as virtual meetings during the COVID-19 pandemic...
Article
Objective(s): This study aimed investigate association of HLA-DRB1 and cytokine polymorphisms with red blood cell(RBC) alloimmunization in Brazilian Myelodysplastic syndrome(MDS) patients with prior exposure to RBC transfusion. Background: MDS patients are at risk RBC alloimmunization due to chronic RBC transfusion. However, differences in immun...
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Background: Hybrid genes are responsible for the formation of Rh variants and are common in patients with sickle cell disease (SCD). However, it is not usually possible to detect them by conventional molecular protocols. In the present study, hybrid genes were investigated using the Quantitative Multiplex Polymerase chain reaction of Short Fluores...
Article
Background/case studies Cromer blood group system consists of 12 high-prevalence and three low-prevalence antigens. The molecular basis for the antigens is known, and the majority is the product of a single nucleotide change in the DAF gene and has been localized to one of the four complement control protein domains on the DAF protein. Antibodies t...
Article
Introdução A transfusão de sangue é uma terapia vital no tratamento e prevenção de algumas complicações das síndromes mielodisplásicas (SMD). No entanto, apesar das transfusões de concentrados de hemácias melhorarem significativamente a qualidade de vida nesta população de pacientes, sua utilização é limitada devido à frequente ocorrência de aloimu...
Article
Background Several centers have selected Black donors to prevent Rh alloimmunization of patients with sickle cell disease (SCD). As the Brazilian population is considered very admixed and race definition by self-declaration is questionable, this study aimed to compare RHCE diversity among patients with SCD and selected groups of Brazilian blood don...
Article
Background The discrimination between weak D types and partial D can be of clinical importance because carriers of partial D antigen may develop anti-D when transfused with D-positive red blood cell units. The aim of this study was to determine by molecular analysis the type of D variants among Brazilian patients requiring transfusions with serolog...
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Background Red blood cell (RBC) alloimmunization is a complication of patients with sickle cell disease (SCD) and it has a greater impact on pregnancy, leading to a risk of hemolytic disease of the newborn and reducing blood availability for pregnant women. This study proposed to evaluate antigen matching transfusion protocols, aiming to reduce RBC...
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Full-text available
Conclusions: The prevalence of blood group antigens and phenotypes varies significantly in Brazil. To ensure a proper rare blood supply, it is essential to establish a local and regional database of rare donors connected to the national registry. The objective of this study was to create a database of rare blood donors in the northern region of so...
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Objective Low levels of neutrophils can be an intrinsic condition, with no clinical consequences or immunity impairment. This condition is the benign constitutional neutropenia (BCN), defined as an absolute neutrophils count (ANC) ≤2000 cells/mm. Diagnosis of BCN is of exclusion where patients are submitted to blood tests and possibly to invasive d...
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Background and objectives: Drug-induced immune hemolytic anemia is a rare condition that occurs primarily because of drug-induced antibodies, either dependent or independent and positive direct antiglobulin test. Our aim was to evaluate the association of positive DAT with nonreactive eluate and DIHA. Materials and methods: From 2014-2018, we ev...
Article
Background: Rh antibodies produced by patients receiving Rh-matched RBC units may be associated with inheritance of altered RH alleles or a result of altered Rh epitopes on donor red blood cells (RBC). On this background, our aim was to evaluate unexpected Rh antibodies in Brazilian patients receiving regular transfusions and determine the clinica...
Article
Background One of the strategies used to reduce the risk of haemolysis due to ABO‐minor incompatible platelet transfusions is to perform a screening test to identify group O donors with high titres of anti‐A and anti‐B. However, critical immunoglobulin M/ immunoglobulin G (IgM/IgG) titres remain unclear. Objective This study aimed to determine IgM...
Article
BACKGROUND A highly reduced expression of Rh antigens in the erythrocyte membrane is the main feature of Rhmod, an extremely rare phenotype. Mutations within RHAG gene, which encodes RhAG glycoprotein and modulates Rh antigen expression and Rh complex formation, are the molecular events responsible for the Rhmod phenotype. Here we report a clinical...
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Full-text available
The prevalence of blood group antigens and phenotypes varies significantly in Brazil. To ensure a proper rare blood supply, it is essential to establish a local and regional database of rare donors connected to the national registry. The objective of this study was to create a database of rare blood donors in the northern region of southern Brazil....
Article
Background: In the novel era of blood group genomics, (re-)defining reference gene/allele sequences of blood group genes has become an important goal to achieve, both for diagnostic and research purposes. As novel potent sequencing technologies are available, we thought to investigate the variability encountered in the three most common alleles of...
Article
The JR blood group system (ISBT 032) consists of one antigen, Jr a , which is of high prevalence in all populations. The rare Jr(a–) phenotype has been found mostly in Japanese and other Asian populations, but also in people of northern European ancestry, in Bedouin Arabs, and in one Mexican. Anti-Jr a has caused transfusion reactions and is involv...
Article
Background and objectives: Weak D phenotypes with very low antigen densities and DEL phenotype may not be detected in RhD typing routine and could be typed as D-negative, leading to D alloimmunization of D-negative recipients. The present study aimed to investigate the presence of RHD-positive genotypes in blood donors typed as D-negative by an au...
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Full-text available
Background: We evaluated different technological approaches and anti-D clones to propose the most appropriate serologic strategy in detecting the largest numbers of D variants in blood donors. Methods: We selected 101 samples from Brazilian blood donors with different expressions of D in our donor routine. The tests were performed in immediate s...
Article
Objectives: The aim of this study was to verify the possibility of performing prophylactic Rh genotype-matching in Brazilian patients with sickle cell disease (SCD) and identify the genotypes that are lacking or insufficient in our donor cohort. Background: Rh alloimmunisation is still a challenge in transfused patients with SCD. Rh genotype-mat...
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The correct identification of erythrocyte antibodies is fundamental for the searching for compatible blood and haemolytic transfusion reactions prevention. Antibodies against antigens of high prevalence are difficult to identify because of the rarity of their occurrence and unavailability of negative red cells for confirmation. We report a case of...
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Background: A notable RHD variability has been observed in Central Argentina's current population attributed to the intermixing of different ethnic groups. The Northwestern region of the country is characterized by a markedly Amerindian genetic contribution. In this sense, the definition of the RHD polymorphism in individuals from this area was la...
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Background: Laboratory testing to identify the molecular basis of serologic weak D phenotypes is recommended to determine whether a pregnant woman or potential transfusion recipient should be managed as RhD-positive or RhD-negative. The variation in D antigen expression on RBCs, different potencies of anti-D typing reagents, lack of standardized t...
Article
Classic methods to determine human red blood cell (RBC) antigens are based on serologic testing, however, there are some limitations. Several molecular techniques have been developed to bypass these restrictions and to detect the most important allelic variants, the last of great value for the approach in the personalized medicine, especially for p...
Article
False-positive and false-negative reactions exist for serological and molecular antigen typing methods. If the predicted phenotype is inconsistent with the patient`s known antibodies or serological phenotype, the discrepancy must be investigated. False-negative and false-positive results are clinically problematic in blood donors and patients. In t...
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Dans les bases de données, les séquences de référence des gènes impliqués dans l’expression des antigènes érythrocytaires sont souvent erronées. Du design des tests jusqu’à l’interprétation des variants rares, ces erreurs peuvent avoir des conséquences en diagnostic. Récemment, une approche par PCR « long-range » couplée au séquençage de nouvelle g...
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Conclusions: This update of the MNS blood group system (Reid ME. MNS blood group system: a review. Immunohematology 2009;25:95-101) reports three new antigens of the MNS system numbered MNS47, MNS48, and MNS49; new glycophorin (GP) variants associated with silent and weak expression of MNS antigens; and the results of new studies on associations o...
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Conclusions: This update of the JR blood group system (Castilho L, Reid MR. A review of the JR blood group system. Immunohematology 2013;29:63-8) reports new ABCG2 alleles encoding Jr(a-) and Jr(a+w/-) phenotypes, the predominant alleles encoding the Jr(a-) phenotype, and new functional aspects of the ABCG2 glycoprotein. The JR blood group system...
Article
Blood banks in developing countries have limited capability to typify common blood groups creating disparities in the access to blood units for patients with rare blood genotypes. We report the case of a Peruvian woman with metastatic breast cancer with KELnull phenotype (K0), a rare blood group characterized by the lack of expression of all Kell a...
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Background and objectives The rare S–s– phenotype has two main molecular backgrounds. GYPB deletions give rise to the S–s–U– phenotype, which loses the expression of the U antigen, while variant GYPB alleles usually lead to the S–s–U+var phenotype, which express a variant U antigen. The S–s– phenotype is typically found in people of African origin...
Article
Background and objectives The International Society of Blood Transfusion (ISBT) Working Party for Red Cell Immunogenetics and Blood Group Terminology meets in association with the ISBT congress and has met three times since the last report: at the international meetings held in Dubai, United Arab Emirates, September 2016 and Toronto, Canada, June 2...
Article
The development of red blood cell (RBC) alloantibodies and autoantibodies complicates transfusion therapy in sickle cell disease (SCD) patients. In an effort to reduce the risk of alloimmunization, some strategies have been used to provide antigen-matched RBC transfusions to patients with SCD in Brazil, including molecular matching in 3 levels: RH...
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Full-text available
Background: Vel is a high frequency blood group antigen and its alloantibody is involved in haemolytic transfusion reactions. After elucidation of the molecular basis of the Vel-negative phenotype defined by a 17-base pair deletion in SMIM1, genotyping has been the technique of choice to identify the Vel-negative phenotype, and molecular investiga...
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Introduction Pre-transfusion tests, essential for the release of blood components, may be affected by drugs. Monoclonal antibodies represent a class of medications increasingly used in the clinical practice, with anti-CD38 monoclonal antibodies (daratumumab) being a promising resource in the treatment of refractory myeloma. This monoclonal antibody...
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Background: Red blood cell (RBC) alloimmunisation is an event that may occur due to factors such as numerous blood transfusions, age, gender and genetic factors such as human leukocyte antigen (HLA). Aims/objectives: The aim of the present study was to investigate the possibility of alloimmunisation to red blood cell group antigens associated wi...
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Full-text available
Background: A considerable number of RHD alleles responsible for weak and partial D phenotypes have been identified. Serologic determination of these phenotypes is often doubtful and makes genetic analysis of RHD gene highly desirable in transfusion recipients and pregnant women. We analyzed the RHD gene in a cohort of pregnant women with doubtful...
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Full-text available
Background: Kell null (K0) individuals can produce anti-Ku, an antibody against many epitopes in the Kell glycoprotein, after transfusion and/or pregnancy. Since sensitized K0 patients are rare, little is known about anti-Ku clinical relevance and in particular about its association to hemolytic disease of the fetus and newborn. Case report: Thi...
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Background: The reason for the difference in susceptibility to red blood cell (RBC) alloimmunization among patients with sickle cell disease (SCD) is not clearly understood and is probably the result of multiple factors. Our hypothesis is that genetic polymorphisms are associated with RBC alloimmunization. Study design and methods: We investigat...
Article
Autoimmune haemolytic anaemia (AIHA) can be classified in different types including warm AIHA (WAIHA), cold haemagglutinin disease (CHAD), mixed-type AIHA, paroxysmal cold haemoglobinuria and drug-induced AIHA. Warm AIHA (WAIHA) is the most common type of AHAI characterized by warm autoantibodies primarily IgG (rarely IgM or IgA) reacting best at 3...
Article
RHD polymorphism shows substantial ethnic variability. In this study, we report the novel RHD exon 3 c.359C>A mutation responsible for the amino acid change p.Ala120Asp found in 28 individuals from Argentina with weak D antigen expression. The RHD*359A allele was only encountered in samples with ccEe phenotype suggesting a genetic cis association b...
Article
The Working Party has met twice since the last report: in Seoul, South Korea 2014, and in London, UK 2015, both in association with the International Society of Blood Transfusion (ISBT) Congress. As in previous meetings, matters pertaining to blood group antigen nomenclature were discussed. Eleven new blood group antigens were added to seven blood...
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Full-text available
Background: As a consequence of the homology and opposite orientation of RHD and RHCE, numerous gene rearrangements have occurred in Africans and resulted in altered RH alleles that predict partial antigens, contributing to the high rate of Rh alloimmunisation among patients with sickle cell disease (SCD). In this study, we characterised variant R...
Article
Background: The high homology and opposite orientation of RH genes promote rearrangements between them and generate a large number of RHD and RHCE variants which can be inherited together. Searching of RHD-CE genotypes predicting partial antigens in donors is of interest in order to find more closely matched donors for African descent patients. Th...
Article
Background: The knowledge of D variants in patients and donors is important because anti-D alloimmunization can occur in some but not all individuals who express a variant RHD allele. Serologic distinction of RhD discrepancies is not always straightforward, which makes molecular analysis highly desirable. Methods: A group of 223 subjects, 129 pa...
Article
Providing blood units for patients with an antibody to a high-prevalence antigen or with multiple common antibodies is a constant challenge to the blood banks. Finding a compatible donor requires extensive screening, with incurs a large amount of investment. In this article, we share our experience of organizing a rare donor inventory with limited...
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Automated testing platforms facilitate the introduction of red cell genotyping of patients and blood donors. Fluidic microarray systems, such as Luminex XMAP (Austin, TX), are used in many clinical applications, including HLA and HPA typing. The Progenika ID CORE XT (Progenika Biopharma-Grifols, Bizkaia, Spain) uses this platform to analyze 29 poly...
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The use of SNaPshot (Applied Biosystems, Foster City, CA) for predicting blood group antigens has emerged as an alternative to hemagglutination testing and also to the current low- and highthroughput blood group genotyping methods. Several groups have developed multiplex-polymerase chain reaction SNaPshot assays to determine single nucleotide polym...