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Publications (30)
Annually, the Philippines is burdened by a high number of infections and deaths due to Dengue. This disease is caused by the Dengue virus (DENV) and is transmitted from one human host to another by the female Aedes aegypti mosquito. Being a developing country, most of the high-risk areas in the Philippines are resource-limited and cannot afford equ...
Among the major classes of plant hormones, auxins, gibberellins, and cytokinins are widely used for plant propagation. These hormones influence the physiological and developmental processes of plants such as root initiation, apical dominance, seed germination, leaf expansion, and development of buds, flowers, and fruits. Its exogenous application w...
Application of nanotechnology in agriculture has expanded to improve crop production. This study was conducted to assess the effects of a nanofertilizer–FertiGroe® N (FG-N) and a nano-plant hormone–HormoGroe® Auxin (HG-A) on the chemical properties and microbial community of Lipa clay loam (CL) and Sariaya sandy loam (SL) soils over a 35-d incubati...
Metal oxide nanoparticles are widely used in many agricultural, medical, and electronic products. This study was conducted to assess the effects of metal oxide nanoparticles on the total culturable soil bacterial population and enzymatic activities-namely, dehydrogenase (DHA), urease (UA), and microbial biomass (MCB)-using Philippine soils. The fac...
In the Philippines, bacterial wilt caused by Ralstonia solanacearum is one of the most important constraints on white potato production. Thus, a DNA-based nanobiosensor kit was developed for the detection of R. solanacearum motility gene. The kit includes a carbohydrate-functionalized magnetic nanoparticles (MNP-F1) and gold nanoparticles conjugate...
Nanomaterials have many beneficial applications; however, their impacts on the environment necessitate an assessment. Understanding any possible negative effects of nano-based products on soil health needs to be performed before their extensive use in crop production and commercialization. Microorganisms are considered the most sensitive indicators...
Carbohydrate-functionalized magnetic nanoparticles (MNP-F1) were used for the capture of the plant pathogens Phytophthora palmivora, Lasiodiplodia theobromae and Ralstonia solanacearum. The first two pathogens are commonly found in cacao with black pod rot and VSD-like symptoms, respectively, and becoming a major constraint in cacao production. On...
Antimicrobial resistance (AMR) is one of the top 10 global public health threats facing humanity, especially in low-resource settings, and requires an interdisciplinary response across academia, government, countries, and societies. If unchecked, AMR will hamper progress towards reaching the United Nations Sustainable Development Goals (SDGs), incl...
The new finding of severe dieback caused by Lasiodiplodia theobromae is becoming a major constraint to cacao production in the Philippines. Early detection of this pathogen will allow the management of disease infections and minimize the risk of the spread of disease. Traditionally, plant pathogen detection requires isolation and morphological eval...
15 N-labeled tryptophan was used to trace the synthesis of indole-3-acetic acid (IAA) by plant growth promoting Klebsiella sp. (PGPB1). Preliminary thin layer chromatography analysis of crude extracts showed that IAA was produced from labeled tryptophan. This is further supported by high pressure liquid chromatography wherein the components of the...
Two nanocellulose-producing bacteria were isolated from separate local nata starter cultures and were characterized to determine their putative identity. The nanocellulose produced by the two bacterial isolates were compared to determine if differences between material properties and in vitro biocompatibility were species-dependent. Nanocellulose m...
The wide applications of gold nanoparticles in fields such as electronics and biomedicine (targeted-drug delivery) have caught the interest of many researchers. In this study, Serratia marcescens NBL1001 was screened for its ability to synthesize gold nanoparticles (AuNP). Colorimetric change from light yellow to purple, 24 h after the
supplementat...
The effect of the combination treatment of 1% chitosan coating and 3-d low temperature conditioning (LTC) at 10 °C was evaluated in maintaining the quality of red-fleshed dragon fruit during storage at 8 °C. Fruit subjected to LTC and chitosan coating treatments had slower respiration rate than the control. Ascorbic acid (AAC) and total phenolic co...
The emergence of multidrug resistant microorganisms ignited interest in silver nanoparticles (AgNPs) and their application as antimicrobial particles. In this study, the synthesis of AgNPs by a wildtype isolate, Serratia sp. NBL1001, and preliminary characterization and antibacterial activity of the produced AgNPs were investigated. Extracellular b...
The emergence of multidrug resistant microorganisms ignited interest in silver nanoparticles (AgNPs) and their application as antimicrobial particles. In this study, the synthesis of AgNPs by a wildtype isolate, Serratia sp. NBL1001, and preliminary characterization and antibacterial activity of the produced AgNPs were investigated. Extracellular b...
Fusarium wilt or Panama disease in banana caused by Fusarium oxysporum has caused severe economic losses in banana industries in the Philippines and other countries, creating an intensive search for solutions to control the pathogen. Recently, it was reported that certain metallic inorganic nanoparticles exhibit excellent biocidal activities agains...
The synthesis of silver nanoparticles (AgNPs) by a wildtype isolate, Serratia sp. NBL1001, its preliminary characterization and antibacterial activity were studied. Extracellular biosynthesis of AgNPs from silver nitrate (AgNO3) was observed by visual inspection of crude cell-free NBL1001 supernatant at 30°C showing color changes of orange-brown ch...
A bacterial isolate, obtained from Philippine soil and able to produce nano-sized gold particles outside the cell, was characterized and identified. The organism’s genus was determined to be Lysinibacillus using conventional cultural, morphological and biochemical tests coupled with the molecular method of 16S rDNA sequencing. The organism was able...
Bacteria-derived crude auxin, cytokinin and gibberellin were isolated from plant gowth-promoting bacteria containing 826.43 ± 3.3mg/g IAA equivalence, 489.66 ± 1.0mg/g Kinetin equivalence and 588.90 ± 6.3mg/g Gibberellic acid equivalence respectively. The isolated plant growth regulators (PGRs) were also successfully encapsulated, using phospholipi...
The synthesis of gold nanoparticles (AuNPs) by bacteria (Isolates NBL1a and NBLB31) under ambient temperature, without pH adjustments and addition of stabilizers, was studied. Extracellular biosynthesis of AuNPs from gold (III) chloride trihydrate (HAuCl4·3H2O) was achieved by the crude cell-free NBL1a and NBLB31 culture supernatants at ambient tem...
Plant-growth-promoting bacteria (PGPB) and actinomycetes isolated from Philippine soils were screened for their ability to extracellularly synthesize gold nanoparticles (Au0) from ionic gold (Au3+). Sixteen isolates, which include 12 PGPBs and four actinomycetes, were tested for the presence of nitrate reductase activity. Nitrate reductase is one o...
One of the constraints to the adoption of plant growth promoting bacteria (PGPB) technology is the lack of information on crop response to PGPB inoculation in different soils. This study examined the effectiveness of PGPB inoculation on yield, shoot and root dry weights, nitrogen and phosphorus uptake of lettuce variety 'Green Tower' grown in Lipa...
Forty plant growth promoting bacteria (PGPB) were screened for gibberellin (GA) production through the dwarf rice (cv. Tanginbozu) bioassay. Among the GA-producing isolates, TCeR 48 produced the highest amount of GA (81.70 mu g GA(3) equiv mL(-1) culture). The peak of GA production by TCeR 48 was at 15 days after inoculation. Extracts from TCeR 48...