Lihui Zeng

Fujian Agriculture and Forestry University | FAFU · Department of Horticulture

A major determinant of fruit production in longan (Dimocarpus longan Lour.) is the difficulty of blossoming. In this study, high-throughput microRNA sequencing (miRNA-Seq) was carried out to compare differentially expressed miRNAs (DEmiRNAs) and their target genes between a continuous flowering cultivar ‘Sijimi’ (SJ), and a unique cultivar ‘Lidongb...

Sugars will eventually be exported transporters (SWEET), a group of relatively novel sugar transporters, that play important roles in phloem loading, seed and fruit development, pollen development, and stress response in plants. Longan (Dimocarpus longan), a subtropic fruit tree with high economic value, is sensitive to cold. However, whether the S...

Longan (Dimocarpus longan Lour.) is a tropical/subtropical fruit tree of significant economic importance. Floral induction is an essential process for longan flowering and plays decisive effects on the longan yield. Due to the instability of flowering, it is necessary to understand the molecular mechanisms of floral induction in longan. In this stu...

Anthocyanins are visual cues for pollination and seed dispersal. They also impart valuable benefits to consumers of fruit. In kiwifruit (Actinidia spp.) studies have shown at least two MYB activators of anthocyanin, while their real function in fruit and mechanisms are not fully understood. Here, transcriptome and small RNA high‐throughput sequenci...

Virus-induced gene silencing (VIGS) is a natural defense mechanism of plants, which can cause sequence-specific degradation of viral RNA and is often used for studying the functional genome. At present, the VIGS system mediated by Tobacco rattle virus (TRV) has been successfully established in many plant species. However, the VIGS system in Chinese...

Background Flavonoid biosynthesis in plants is primarily regulated at the transcriptional level by transcription factors modulating the expression of genes encoding enzymes in the flavonoid pathway. One of the most studied transcription factor complexes involved in this regulation consists of a MYB, bHLH and WD40. However, in Chinese Narcissus ( Na...

In fruit trees, flowering is a key event followed by fruit development and seed production. Gigentea ( GI ), a clock-associated gene, is known to contribute to photoperiodic flowering and circadian clock control in Arabidopsis thaliana . However, its functions in woody fruit trees remain unclear. In this study, a 2000 bp promoter fragment of the lo...

Flowering is an important biological process for plants that ensures reproductive success. The onset of flowering needs to be coordinated with an appropriate time of year, which requires tight control of gene expression acting in concert to form a regulatory network. MicroRNAs (miRNAs) are non-coding RNAs known as master modulators of gene expressi...

Carbohydrates are nutrients and important signal molecules in higher plants. Sugar transporters (ST) play important role not only in long-distance transport of sugar, but also in sugar accumulations in sink cells. Longan (Dimocarpus longan L.) is one of the most important commercial tropical/subtropical evergreen fruit species in Southeast Asia. In...

The purpose of this study was to establish an extraction method for the kinsenoside compound from the whole plant Anoectochilus roxburghii. Ultrasound assisted extraction (UAE) and Ultra-high performance liquid chromatography (UPLC) method were used to extract and determine the content of kinsenoside, while response surface method (RSM) was used to...

R2R3-MYB transcription factors play important roles in the regulation of plant flavonoid metabolites. In the current study, NtMYB3, a novel R2R3-MYB transcriptional factor isolated from Chinese narcissus (Narcissus tazetta L. var. chinensis), was functionally characterized. Phylogenetic analysis indicated that NtMYB3 belongs to the AtMYB4-like clad...

High temperatures are known to reduce anthocyanin accumulation in a number of diverse plant species. In potato (Solanum tuberosum L.), high temperature significantly reduces tuber anthocyanin pigment content. However, the mechanism of anthocyanin biosynthesis in potato tuber under heat stress remains unknown. Here we show that high temperature caus...

Chinese narcissus (Narcissus tazetta L. var. chinensis Roem.) is a popular flower in Asia. However, flower colors are limited with all cultivars having a white perianth and yellow corona. Previous studies have shown no anthocyanin accumulation in this species. The reason for an absence of anthocyanins remains unknown, with the flavonoid biosyntheti...

A R2R3-MYB-like gene，named NtMYB5，was selected from transcriptome of Narcissus tazetta var. chinensis and was cloned in order to study its function in flavonoid metabolic pathway. Multiple alignments showed that NtMYB5 contained R2-domain and R3-domain. A pdLNLD/ELxiG/S motif was also found near the C-terminal. Phylogenetic tree analysis indicated...

The full length of cDNA sequences of two ELF4 homolog genes were cloned from Dimocarpus longan 'Red Seed', named DlELF4-1 and DlELF4-2. The functions of these two genes were analyzed by bioinformatics analysis and real-time quantitative PCR. To further study the function of the DlELF4 genes, DlELF4-1 and DlELF4-2 were overexpressed in Arabidopsis t...

R2R3 MYB transcription factors play key functions in the regulation of secondary metabolites. In the present study, a R2R3 MYB transcriptional factorNtMYB2was identified from Chinese narcissus (Narcissus tazettaL. var.ChinensisRoem) and functionally characterized.NtMYB2belongs to subgroup 4 of the R2R3 MYB transcription factor family that are relat...

Longan (Dimocarpus longan Lour.) is a subtropical fruit tree of significant economic importance. Flowering is a key event in longan and other fruit trees. In this study, GIGANTEA (GI) and FLAVIN-BINDING, KELCH REPEAT, F-BOX1 (FKF1) homologs were cloned from longan and named DlGI and DlFKF1, respectively. Translated sequences of DlGI and DlFKF1 reve...

Longan (Dimocarpus longan L.) is a tropical/subtropical fruit tree of significant economic importance in Southeast Asia. However, a lack of transcriptomic and genomic information hinders research on longan traits, such as the control of flowering. In this study, high-throughput RNA sequencing (RNA-Seq) was used to investigate differentially express...

Callus induction and regeneration from ovary, pedicel, and scape explants of Chinese narcissus (Narcissus tazetta L. var. chinensis Roem.) ‘Jin Zhan Yin Tai’ were investigated. Two types of callus, compact and friable, were obtained, but only compact callus could differentiate into shoots. Initially, compact callus was produced on 1.0 Murashige and...

An ortholog of LEAFY, LLFY (longan LFY) was isolated the floral buds of longan (Dimocarpus longan L.) trees. The LLFY gene consists of a 1,167-bp open reading frame and encodes a putative protein of 389 amino acids, which are 62.5% identical to LFY and 76.98% to csLFY (the homolog of LFY from Citrus sinensis). RT-PCR analysis showed that LLFY trans...

Ponkan mandarin (Citrus reticulata Blanco) is one of the most important commercial cultivars of mandarin orange in China. This study reports an improved and efficient protocol for in vitro plant regeneration of Ponkan mandarin. Epicotyl segments, which were cut longitudinally into two halves, were used as explants. The shoot regeneration frequency...

In plants, Glycoside Hydrolase (GH) Family 1 beta -glycosidases are believed to play important roles in many diverse processes including chemical defense against herbivory, lignification, hydrolysis of cell wall-derived oligosaccharides during germination, and control of active phytohormone levels. Completion of the Arabidopsis thaliana genome sequ...