Lihong Wang Bishop

Lihong Wang Bishop
Vanderbilt University | Vander Bilt · Department of Biomedical Engineering

PhD

About

43
Publications
4,092
Reads
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919
Citations
Citations since 2016
25 Research Items
770 Citations
2016201720182019202020212022050100150
2016201720182019202020212022050100150
2016201720182019202020212022050100150
2016201720182019202020212022050100150
Additional affiliations
November 2018 - present
Vanderbilt University
Position
  • Professor (Assistant)
November 2013 - November 2018
Vanderbilt University
Position
  • PostDoc Position
November 2010 - November 2013
Vanderbilt University
Position
  • Research Intern
Education
September 2010 - June 2013
Xiamen University
Field of study
  • Physiology and cancer biology
September 2005 - June 2008
Nantong University
Field of study
  • Biochemistry and molecular biology

Publications

Publications (43)
Article
Full-text available
Unfolded protein response (UPR) protects malignant cells from endoplasmic reticulum stress-induced apoptosis. We report that Aurora kinase A (AURKA) promotes cancer cell survival by activating UPR in esophageal adenocarcinoma (EAC). A strong positive correlation between AURKA and binding immunoglobulin protein (BIP) mRNA expression levels was found...
Article
Traditional approaches to cancer vaccines elicit weak CD8⁺ T cell responses and have largely failed to meet clinical expectations. This is in part due to inefficient antigen cross-presentation, inappropriate selection of adjuvant and its formulation, poor vaccine pharmacokinetics, and/or suboptimal coordination of antigen and adjuvant delivery. Her...
Article
Full-text available
When compartmentally mislocalized within cells, nucleic acids can be exceptionally immunostimulatory and can even trigger the immune-mediated elimination of cancer. Specifically, the accumulation of double-stranded DNA in the cytosol can efficiently promote antitumor immunity by activating the cGAMP synthase (cGAS) / stimulator of interferon genes...
Article
Full-text available
The stimulator of interferon genes (STING) pathway plays an important role in the immune surveillance of cancer and, accordingly, agonists of STING signaling have recently emerged as promising therapeutics for remodeling of the immunosuppressive tumor microenvironment (TME) and enhancing response rates to immune checkpoint inhibitors. 2′3′-cyclic g...
Article
Full-text available
Background Neuroblastoma (NB) is a childhood cancer for which new treatment options are needed. The success of immune checkpoint blockade in the treatment of adult solid tumors has prompted the exploration of immunotherapy in NB; however, clinical evidence indicates that the vast majority of NB patients do not respond to single-agent checkpoint inh...
Article
Full-text available
Tissue-resident memory T cells (TRM) patrol non-lymphoid organs and provide superior protection against pathogens that commonly infect mucosal and barrier tissues, such as the lungs, intestine, liver, and skin. Thus, there is a need for vaccine technologies that can induce a robust, protective TRM response in these tissues. Nanoparticle (NP) vaccin...
Article
Full-text available
TFF1, a secreted protein, plays an essential role in keeping the integrity of gastric mucosa and its barrier function. Loss of TFF1 expression in the TFF1-knockout (KO) mouse leads to a pro-inflammatory phenotype with a cascade of gastric lesions that include low-grade dysplasia, high-grade dysplasia, and adenocarcinomas. In this study, we demonstr...
Conference Paper
Background & Aims: Amplifications and mutations of KRAS play essential roles in resistance to chemotherapeutics in luminal gastrointestinal cancers. We investigated the regulation of ribosomal protein S6 kinase B1 (RS6KB1) by AURKA and the effects of knockdown AURKA or its inhibitions using alisertib, an AURKA inhibitor, in human gastrointestinal c...
Conference Paper
Background & Aims: Amplifications and mutations of KRAS play essential roles in resistance to chemotherapeutics in luminal gastrointestinal cancers. We investigated the regulation of ribosomal protein S6 kinase B1 (RS6KB1) by AURKA and the effects of knockdown AURKA or its inhibitions using alisertib, an AURKA inhibitor, in human gastrointestinal c...
Article
Full-text available
Correction for ‘The efficiency of cytosolic drug delivery using pH-responsive endosomolytic polymers does not correlate with activation of the NLRP3 inflammasome’ by Jessalyn J. Baljon et al. , Biomater. Sci. , 2019, DOI: 10.1039/c8bm01643g.
Article
Inefficient cytosolic delivery has limited the development of many promising biomacromolecular drugs, a long-standing challenge that has prompted extensive development of drug carriers to facilitate endosomal escape. Although many such carriers have shown considerable promise for cytosolic delivery of a diversity of therapeutics, destabilization or...
Article
RNA agonists of the retinoic acid gene I (RIG-I) pathway have recently emerged as a promising class of cancer immunotherapeutic, but their efficacy is hindered by drug delivery barriers, including nuclease degradation, poor intracellular uptake, and minimal access to the cytosol where RIG-I is localized. Here, we explore the application of pH-respo...
Article
Background & aims: Activation of KRAS signaling and overexpression of the aurora kinase A (AURKA) are often detected in luminal gastrointestinal cancers. We investigated regulation of ribosomal protein S6 kinase B1 (RPS6KB1) by AURKA and the effects of alisertib, an AURKA inhibitor, in mice xenograft tumors grown from human gastrointestinal cancer...
Article
Purpose: We aimed to investigate the therapeutic efficacy of single agent and the combination of quinacrine (QC) and suberoylanilide hydroxamic acid (SAHA) in wt- and mut-p53 upper gastrointestinal cancer (UGC) cell models. Experimental design: ATP-Glo, clonogenic survival, Annexin V, comet, DNA double-strand breaks (DSB), quantitative real-time...
Article
Full-text available
De novo and acquired resistance to platinum therapy such as cisplatin (CDDP) is a clinical challenge in gastric cancer treatment. Aberrant expression and activation of Aurora kinase A (AURKA) and eukaryotic translation initiation factor 4E (eIF4E) are detected in several cancer types. Herein, we investigated the role of AURKA in CDDP resistance in...
Article
Purpose: In this study, we investigated the role of Aurora kinase A (AURKA) in regulating EIF4E, cap-dependent translation, and resistance to mTOR inhibitor, RAD001 (everolimus). Experimental design: Tumor xenografts and in vitro cell models of upper gastrointestinal adenocarcinomas (UGCs) were used to determine the role of AURKA in activation o...
Article
Purpose: The bromodomain and extra-terminal domain (BET) family proteins are epigenetic readers for acetylated histone marks. Emerging BET bromodomain inhibitors have exhibited antineoplastic activities in a wide range of human cancers through suppression of oncogenic transcription factors, including MYC. However, the preclinical activities of BET...
Article
The canonical WNT signaling is aberrantly activated in the vast majority of colorectal cancer (CRC) and results in activation of beta-catenin and its downstream target MYC. These two oncogenic transcription factors play central roles in CRC pathogenesis, but are refractory to direct pharmacological interventions. The recurrent mutations of APC or β...
Article
Full-text available
The mucus layer coating the gastrointestinal tract serves as the first line of intestinal defense against infection and injury. Probiotics promote mucin production by goblet cells in the intestine. p40, a Lactobacillus rhamnosus GG (LGG)-derived soluble protein, has been shown to transactivate the EGF receptor (EGFR) in intestinal epithelial cells,...
Article
Full-text available
Macrophages regulate innate immunity to maintain intestinal homeostasis and play pathological roles in intestinal inflammation. Activation of the epidermal growth factor receptor (EGFR) promotes cellular proliferation, differentiation, survival, and wound closure in several cell types. However, the impact of EGFR in macrophages remains unclear. Thi...
Article
Full-text available
Berberine, an isoquinoline alkaloid, is an active component of Ranunculaceae and Papaveraceae plant families. Berberine has been found to suppress growth of several tumor cell lines in vitro through the cell-type-dependent mechanism. Expression and activation of epidermal growth factor receptor (EGFR) is increased in colonic precancerous lesions an...
Article
Full-text available
Berberine, an isoquinoline alkaloid derived from plants, is a traditional medicine for treating bacterial diarrhea and intestinal parasite infections. Although berberine has recently been shown to suppress growth of several tumor cell lines, information regarding the effect of berberine on colon tumor growth is limited. Here, we investigated the me...
Data
Berberine induces cell death and LDH release in HT-29 cells. HT-29 cells were cultured in serum-starved medium at 37°C for 24 h (A and B) or 36 h (C and D) with or without berberine (50 µM) treatment for indicated times from the end of experiment. Cell viability (A and C) and LDH release (B and D) were detected as described in Figure 2. Data repres...
Data
Berberine does not stimulates capase-dependent apoptosis in colon tumor cells. Cells were cultured in serum-starved RPMI 1640 medium at 37°C for 24 hours with or without berberine at 50 µM treatment for indicated times or TNF (100 ng/ml) and cycloheximide (1 µg/ml) for 6 hours from the end of the experiment. Cellular lysates were collected for West...
Data
Berberine stimulates AIF release in IMCE, which is required for berberine-induced cell death in HT29 cells. Cells were treated with berberine at 75 µM in serum-starved medium at 37°C for 24 h, as described in Figure 2. Cytoplasmic, nuclear, and mitochondrial fractions were prepared for Western blot analysis using anti-AIF, anti-GAPDH (cytosolic mar...
Data
Absorption of berberine by IMEC and YAMC cells. Cells were treated with berberine for 18 h at indicated concentrations (A) or at 50 µM for indicated times (B), as described in Figure 2. Cells were homogenized in PBS and the cellular soluble protein concentration was determined. Berberine content in the soluble fraction was determined by positive io...
Data
Berberine-induced cell death in IMEC cells was detected by Annexin V and propidium iodide (PI) staining. IMEC ells were treated with berberine (50 µM) in serum-starved medium at 37°C for 24 h. Then cells were dissociated using accutase and double stained with Annxin V-FITC and PI. The percentage of cells positive for Annexin V and PI was detected b...
Data
Tiron inhibits berberine-induced ROS production in IMEC cells. Cells were treated with berberine (50 µM) in serum-starved medium at 37°C for 18 h, as described in Figure 2, in the presence or absence of a ROS scavenger, Tiron (5 µM). Intracellular ROS levels were detected using DHE probe and the amount of fluorescence was measured using flow cytome...
Article
Full-text available
Inflammatory bowel disease (IBD) results from dysregulation of intestinal mucosal immune responses to microflora in genetically susceptible hosts. A major challenge for IBD research is to develop new strategies for treating this disease. Berberine, an alkaloid derived from plants, is an alternative medicine for treating bacterial diarrhea and intes...
Article
Full-text available
Berberine, an isoquinoline alkaloid derived from plants, is a traditional medicine for treating bacterial diarrhea and intestinal parasite infections. Although berberine has recently been shown to suppress growth of several tumor cell lines, information regarding the effect of berberine on colon tumor growth is limited. Here, we investigated the me...
Article
Hypoxia has been shown to increase the expression of a variety of proteins involved in iron homeostasis, including ceruloplasmin, transferrin, and transferring receptor. Divalent metal transporter 1 (DMT1) is a transmembrane protein that is important in divalent metal ion transport, in particular iron. Although previous studies have provided that D...

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