Lidia Robaina

University of Las Palmas de Gran Canaria | ULPGC · Department of Biology

The interaction between vitamin D and vitamin K is crucial for regulating bone metabolism and maintaining calcium homeostasis across diverse animal species due to their complementary roles in calcium metabolism and bone health. However, research on this interaction of vitamin D and K in fish, particularly Mediterranean species like gilthead seabrea...

Simple Summary Sustainable aquaculture expansion will be crucial for the food security of a growing population expected to reach ~10 billion by 2050. Larval rearing is one of the most complex phases of marine aquaculture due to the need for additional facilities and labor to produce zooplankton to feed the larvae. Therefore, establishing adequate p...

The high worldwide amount of residues derived from the banana harvest is an opportunity to create synergies between the banana industry and aquaculture, from a circular economy perspective. The present work objective is to evaluate the inclusion of banana by-products, such as banana pseudo-stem and banana flower in tilapia feeds, and to explore the...

Mullets (Mugilidae) present significant potential for sustainable aquaculture diversification due to their eurythermal, euryhaline, and low-trophic nature. However, the physiological differences and optimal cultured conditions among the diverse mullet species are quite unknown. For these reasons, the present study aimed to address two main objectiv...

The golden grey mullet (Liza aurata) is a promising species for aquaculture's sustainable expansion. However, the lack of sustainable juvenile provision, mainly related to the lack of reproductive control, is one of the most significant bottlenecks for further expanding the culture of this species. In many cases, mullet broodstock management needs...

Heavy metals have become subject of concern in the recent years because of its potency to cause cardiovascular diseases and other toxic health effects. Therefore, this research was assumed to investigate the level of toxicity in terms of heavy metals accumulation in the fish samples and its benefits and risk for human consumers health and also eval...

Dietary hydroxy-selenomethionine (OH-SeMet) reduces oxidative stress and modulates immune response against bacterial infection in fish. However, the effect of OH-SeMet on essential fatty acids with a high oxidation risk or on the response against viral infection has not been sufficiently studied. This study aimed to assess the impact of dietary OH-...

The aim of this work is the optimization of phenolic compound extraction from three by-products of banana crops (rachis, discarded banana, and banana’s pseudostem pulp), as a way to valorize them through a green extraction process. The influence of the temperature and aqueous ethanol concentration (Et-OH) on extract properties (total phenol content...

Among of the actual challenges for the sustainable aquaculture development are to lower the trophic level of the cultured species, and to increase the use of by-products from the primary sector. The golden mullet (Liza aurata) is a marine low trophic level consumer present in the Mediterranean and in the Canary Islands, where there was a significan...

A preliminary approximation to characterizes the banana culture by‐products, like banana flowers and banana pseudo‐stem, including a comprehensive analysis of the phenolic compounds by HPLC‐MS was performed in this study. The results suggest that both materials showed to have a potential for their use as novel food ingredients. image

Environmental factors such as nutritional interventions during early developmental stages affect and establish long-term metabolic changes in all animals. Diets used during the spawning period causes a nutritional programming effect in offspring of gilthead sea bream and affects long-term metabolism. Studies showed modulation of genes such as fads2...

Manganese (Mn) is an essential metal for fish and requirements have been established for several finfish but not for gilthead sea bream. Thus, the present study aims to establish the optimal dietary supplementation level of Mn in gilthead sea bream fingerlings fed vegetable based diets. Gilthead sea bream fingerlings (weight 12.6 ± 1.5 g, mean ± S....

Replacement of fish meal (FM) and oil (FO) by plant sources can alter the feed mineral profile. However, requirements of these nutrients have not been established for gilthead sea bream. The aim of the present study was to obtain information about the adequate levels of some of these minerals (such as Zn, Cu, Mn, Se, Co, Fe and Ca) in diets with lo...

A 13-week feeding trial was carried out with juvenile rainbow trout to test two diets: a control diet without astaxanthin (AX) supplementation (CTRL diet), and a diet supplemented with 100 mg/kg of synthetic AX (ASTA diet). During the last week of the feeding trial, fish were exposed to episodic hyperoxia challenge for 8 consecutive hours per day....

A 13-week feeding trial was carried out with juvenile rainbow trout to test two diets: a control diet without astaxanthin (AX) supplementation (CTRL diet), and a diet supplemented with 100 mg/kg of synthetic AX (ASTA diet). During the last week of the feeding trial, fish were exposed to episodic hyperoxia challenge for 8 consecutive hours per day....

Substitution of marine ingredients (FM‐FO) by plant protein and oil sources can modify selenium (Se) levels in feeds. Se plays an important role in the antioxidative defence by forming part of selenoproteins. Se requirements of gilthead sea bream are not accurately determined; therefore, this study was conducted to define Se supplementation levels...

Whereas wild specimens exhibit a red pink silver colour, under captivity red porgy (Pagrus pagrus) skin turns dark grey, being directly associated with lower acceptance by the consumers and lower market value. This study was conducted to evaluate the effect of diet supplementation with two carotenoid sources, at two different concentrations, on gro...

Fish and feed waste provide most of the nutrients required by the plants in aquaponics if the optimum ratio between daily fish feed inputs and the plant growing area is sustained. Thus, the fish feed needs to fulfil both the fish’s and plant’s nutritional requirements in an aquaponic system. A controlled fish waste production strategy where the nit...

The present study aimed to determine the effects of dietary inclusion of selenium (Se) in the form of either inorganic Se (sodium selenite, NaSe) or organic Se (hydroxy-selenomethionine, OH-SeMet) on gilthead seabream (Sparus aurata). Triplicate groups of 150 fish each (6.2 ± 0.04 g initial body weight) were distributed in 15 tanks and fed one of f...

Fish meal is increasingly substituted in diets for gilthead sea bream with ingredients of terrestrial origin which may affect the mineral content and availability. Among these minerals, copper (Cu) is an essential trace element whose excess may have a potential toxic effect. Since ingredients of terrestrial origin have higher Cu levels than marine...

The use of vegetable ingredients may alter the mineral balance of feeds for gilthead seabream (Sparus aurata) due to the differences in mineral composition between marine and vegetable ingredients. Whereas seabream fed low levels of zinc (Zn) and manganese (Mn) may present reduced growth and performance, as well as alterations in mineral deposition...

Terrestrial protein and lipid sources are commonly used as substitutes for marine fishery-derived raw ingredients in fish diets. However, their use is related with several side-effects on marine fish performance, health, or disease resistance. Physical barriers of the skin, gills, and gut constitute the primary defense mechanism of fish. Skin mucus...

Meagre (Argyrosomus regius) is a promising new aquaculture species, however with some bottlenecks to high-scale commercial production. One of those constraints are the scarce information about its nutritional requirements, which difficult the production of optimized diets and can lead to pathological alterations in liver, such as hepatic steatosis....

Traditionally, a major part of aquaculture technology requires fish oil (FO) and fish meal (FM) to produce the aquafeed for farmed species. FO is the main source of persistent organic pollutants (POPs) in fish feed. In recent years, the use of vegetable-origin ingredients in fish feeds has been increasingly studied as an alternative to reduce the l...

Systemic granulomatosis is a growing disease with a high morbidity, which affects to the majority of farmed meagre (Argyrosomus regius). The impossibility of isolating any infectious agents has hypothesized a nutritional origin of the disease. In order to try to elucidate the nutritional origin of granulomas, juvenile meagre were fed for 15 weeks w...

To describe the effect and the distribution of zinc in different tissues of a representative marine fish species, gilthead seabreams (Sparus aurata Linnaeus, 1758) were fed different diets containing zinc in different sources (organic, inorganic, encapsulated, blood‐rich diet or fishmeal). The effect was monitored by histology in the lens, liver an...

Meagre (Argyrosomus regius) is a promising new aquaculture species, with great potential due to its high growth rate, feed efficiency and easy adaptation to captivity. Essential fatty acids (EFA) are required to sustain growth, development, immune status and survival (Watanabe, 1982; Izquierdo, 2005). EFA requirement of meagre fingerlings were prev...

Aquaponics can be defined as the integration of hydroponic plant production in a recirculating aquaculture system and has been proposed as a sustainable method to control the accumulation of waste produced by fish farming. The objective of the present study was to determine the biomass production and its feed potential of Myriophyllum aquaticum, Li...

Aquaponics can be defined as the integration of hydroponic plant production in a recirculating aquaculture system and has been proposed as a sustainable method to control the accumulation of waste produced by fish farming. The objective of the present study was to determine the biomass production and its feed potential of Myriophyllum aquaticum, Li...

The aim of this study was to evaluate the effects of increasing dietary arachidonic acid (ARA) levels (from 1 to 6% of total fatty acids) on European sea bass (Dicentrarchus labrax) juveniles’ growth performance, tissue fatty acid profile, liver morphology as well as long-chain polyunsaturated fatty acids (LC-PUFA) biosynthesis, triglyceride and ch...

Systemic granulomatosis is a growing disease with a high morbidity, which affects to the majority of farmed meagre (Argyrosomus regius). The impossibility of isolating any infectious agents has hypothesized a nutritional origin of the disease. In order to try to elucidate the nutritional origin of granulomas, juvenile meagre were fed for 15 weeks w...

https://idus.us.es/xmlui/handle/11441/68953 Libro sobre pigmentos carotenoides editado en el marco de la RED TEMÁTICA IBERCAROT (referencia 112RT445) http://carotenoides.us.es. PROGRAMA IBEROAMERICANO DE CIENCIA Y TECNOLOGIA PARA EL DESARROLLO – CYTED.

Substituting fishmeal (FM) with vegetable meal (VM) can markedly affect the mineral composition of feeds, and may require additional mineral supplementation. Their bioavailability and optimal supplementation levels depend also on the form of delivery of minerals. The aim of the study was to determine the effect of different delivery forms of three...

Vertebral morphology of Gilthead sea bream juveniles Vertebrae 3–6 and 13–16 were measured for length, height and vertebral length/vertebral height. Measurements were carried for fish fed a diet based on vegetable ingredients (C-) without mineral supplementation and fish fed a diet based on fish meal and fish oil. Vertebra 13 was the only vertebra...

Nutrition during periconception and early development can modulate metabolic routes to prepare the offspring for adverse conditions through a process known as nutritional programming. In gilthead sea bream, replacement of fish oil (FO) with linseed oil (LO) in broodstock diets improves growth in the 4-month-old offspring challenged with low-FO and...

The aim of this study was to assess the effects of low levels of dietary fish meal (FM) and fish oil (FO) on disease resistance and gut associated lymphoid tissue (GALT) response after an experimental intestinal infection with V. anguillarum in European sea bass (Dicentrarchus labrax) For that purpose, sea bass juveniles were fed one of four diets...

The main objective of this study was to assess the effects of graded levels of dietary arachidonic acid (ARA), supplemented from alternative sources, on fatty acid composition of plasma and head kidney leucocytes of European sea bass (Dicentrarchus labrax). For that purpose, sea bass juveniles were fed four diets containing graded levels of ARA as...

The aim of this study was to evaluate the combination of maximum replacement of fishmeal (FM) and fish oil (FO) by alternative meals (AM) and oils (AO) to develop nutritious, but more eco-friendly diets for European sea bass (Dicentrarchus labrax) juveniles. European sea bass of 9.8 ± 1.5 g and 9.1 ± 0.5 cm were fed nine isonitrogenous (45% crude p...

Aquaponics is the combination of aquaculture (fish) and hydroponic cultivation of plants. This review examines fish welfare in relation to rearing water quality, fish feed and fish waste and faeces to develop a sustainable aquaponic system where the co-cultured organisms, fish, bacteria in biofilters and plants, should be considered holistically in...

The main objective of this study was to assess the effects of graded levels of replacement of fishmeal (FM) and fish oil (FO) by terrestrial meals (TM) and vegetable oils (VO) on gut health of European sea bass (Dicentrarchus labrax) based on gut morphology, gut-associated immune system (GALT) and gut microbiota composition. Reducing the dietary FM...

Seven experimental isonitrogenous (50%) and isolipidic (16%) diets with different levels of α-tocopherol acetate (16, 100, 190, 285, 430, 880 and 1300 mg kg− 1) were tested during 72 days to evaluate growth performance, tissue composition, fillet oxidation and liver histology in meagre juveniles, Argyrosomus regius. Growth performance, feed convers...

Special feeds for tilapia have been stated as one of the bottleneck for the aquaponic development in Europe. Local resources valorization is needed to formulate better sustainable feeds at low-medium production scale. Wine production in Europe annually increased with the subsequent higher waste generated from the used grapes. Grapefruit (Vitis vini...

Little is known about the nutritional requirements of ornamental fish and the effects which they may have on fish histology. Because of this, 45 young, captive-bred seahorses (1.37 g ± 0.51) were randomly divided and put into fifteen, 35-L, glass aquariums. Three experimental diets were tested in triplicate: live enriched Artemia (LEA); frozen enri...

The study assesses the effects of dietary concentrated mannan oligosaccharides (cMOS) on fish performance, biochemical composition, tissue fatty acid profiles, liver and posterior gut morphology and gen expression of selected parameters involved on the intestinal immune response and liver lipid metabolism of European sea bass (Dicentrarchus labrax)...

The short-snouted seahorse (Hippocampus hippocampus) is a benthic fish that bases its survival on camouflage with the environment; therefore, both in wild and in captivity specimens, differences in coloration have been observed. This study was conducted to study the effect of five different diets, based on Artemia and frozen mysis, on fish survival...

Two trials were conducted to evaluate the nutrient digestibility of river crab (RC) (Procambarus clarkii) and marine crab (MC) (Chaceon affinis) meals in diets for red porgy (Pagrus pagrus) and the effect on ammonia-N excretion and P and Ca retentions. In trial I, the apparent digestibility coefficient (ADC) of nutrients and energy contents in RC a...

The present study was conducted to evaluate the effect of feeding marine crab (SMC) meal and sea urchin (SU) meal on growth performance, ammonia-N excretion, skin colouration and final flesh quality and lipid oxidation of red porgy (Pagrus pagrus). Fish were fed with five diets: a control-diet (CD); two diets replacing 10% and 20% fish meal protein...

The present study was conducted to evaluate the effect of using river crab (Procamburus clarkii) meal (RC) and marine crab (Chaceon affinis) meal (MC) on the red porgy (Pagrus pagrus) fillet fatty acid profile and quality. Red porgy were fed during 193 days with five diets: a control diet based on high-quality fish meal (CD); and four diets replaci...

The expression and regulation of intestinal oligopeptide transporter (PepT)-1 when vegetable sources are used as a substitute for fish meal in the diet of marine fish has not yet been explored. In the present study, as part of our ongoing work on elucidating PepT1 gene expression in relation to different dietary treatments, we have now isolated and...

The objective of the present study was to determine and compare the activity of digestive lipases BAL and PLA2 in red porgy juveniles fed diets with differing protein to lipid ratios, as indicators of diet utilization and growth performance response.

Two females bearing oocytes larger than 500μ and four fluent males were selected for injection of 20 μg.kg-1 GnRHa (Sigma-Aldrich Co. St. Louis, Missouri, USA). Broodstock, kept in 10 m3 tanks, under natural temperature and photoperiod conditions in the Canary Islands produced a high spawn quality: 92.28% fertilized eggs, 55.48% viable eggs, 77.76%...

El objetivo del presente estudio fue la obtención de puestas viables mediante inducción con GnRHa, de reproductores, capturados en el medio natural y mantenidos en condiciones de cultivo de S. rivoliana, y de la determinación de la calidad de las mismas.

Two females bearing oocytes larger than 500μ and four fluent males were selected for induction with successive injections of 20 μg.kg-1 GnRHa (Sigma-Aldrich Co. St. Louis, Missouri, USA). Broodstock kept in 10 m3 tanks, under natural temperature and photoperiod conditions in the Canary Islands. A total of twenty-two spawns were obtained with fiftee...

Two females with oocytes bigger than 500μ, and four fluent males, originally captured from the wild and acclimated to the broodstock of the GIA were used for this study. Fish were acclimated in tanks of 10 m3, and they were injected three times per month (June till November) with 20μg.kg-1 of GnRha. A total of 33 spawns with 15 injections were obta...

El objetivo del presente estudio fue la obtención de puestas viables, mediante inducción con GnRHa, de reproductores, capturados en el medio natural y mantenidos en condiciones de cultivo de S. dumerili, y de la determinación de su producción

Two females with oocytes bigger than 500μ, and four fluent males, were acclimated in a tank of 10 m3, and were injected three times per month (June till November) with 20μg.kg-1 of GnRha. The number of eggs obtained ranged from a minimum of 1,680,500 in June and a máximum of 6,699,000 in September, and the total was of 20,189,700. A positive relati...

A 24-week feeding trial was conducted to study the possible effect of dietary canthaxanthin on red porgy growth and lipid composition. Two triplicate groups were established to test two experimental diets: (1) Control group fed a diet with no added carotenoids, and (2) canthaxanthin group (CTX100) fed a diet with 100 mg of synthetic canthaxanthin p...

A 24-week feeding trial was conducted to study the possible effect of dietary canthaxanthin on red porgy growth and lipid composition. Two triplicate groups were established to test two experimental diets: (1) Control group fed a diet with no added carotenoids, and (2) canthaxanthin group (CTX100) fed a diet with 100 mg of synthetic can-thaxanthin...

The objective of this study was to evaluate three products derived from krill as sources of essential fatty acids, protein and, particularly, phospholipids in microdiets for larval gilthead seabream (Sparus aurata). Their effect on larval performance, biochemical composition and histological development was investigated. The addition of krill phosp...

Despite carotenoids and essential fatty acids seem to play important roles in fish reproduction, no studies have yet been conducted to determine the effect of dietary carote-noids on gilthead seabream broodstock performance and their relation as antioxidants with dietary n-3 highly unsat-urated fatty acid (HUFA) levels. In addition, the high cost o...

A 120-day feeding trial was conducted to assess the possible effect of including dietary astaxanthin (ASTX), for different lengths of time prior to harvest, on red porgy growth performance, lipid and fatty acid composition and post-mortem skin colour. Four treatment groups were established with fish of initial weight of approximately 220g. Control...

A 120-day feeding trial was conducted to assess the possible effect of including dietary astaxanthin (ASTX), for different lengths of time prior to harvest, on red porgy growth performance, lipid and fatty acid composition and post-mortem skin colour. Four treatment groups were established with fish of initial weight of approximately 220 g. Control...

River crab (RC) meal (Procambarus clarkii) and marine crab (MC) meal (Chaceon affinis) were tested as a partial replacement for fish meal in diets for red porgy (Pagrus pagrus), and their effects on growth performance, fish proximate composition and skin colouration were evaluated. Red porgy were fed during 165 days with five diets. High-quality fi...

Increased energy content in fish feeds has led to an enhanced fat deposition, particularly in European sea bass, concerning fish farmers. Inclusion of conjugated linoleic acid (CLA) could reduce fat deposition as in other vertebrates. To determine if dietary CLA affects fat deposition, lipid metabolism, lipid composition and morphology of different...

Effect of dietary substitution of fish meal for marine crab and echinoderm meals on growth performance, ammonia excretion, skin colour, and flesh quality and oxidation of red porgy (Pagrus pagrus) The present study was conducted to evaluate the effect of feeding marine crab (SMC) meal and sea urchin (SU) meal on growth performance, ammonia-N excret...

The Delta6 and Delta5 desaturases and elongases show only very limited activity in marine fish, and little is known of the possibility of enhancing Delta6 desaturase gene expression in these fish. The use of plant oils in marine fish diets is limited by their lack of n-3 highly unsaturated fatty acids (HUFA) despite an abundant content of the 18C f...

Two feeding trials were conducted to determine the minimum dietary protein level producing maximum growth, and the optimum protein to energy ratio in diets for red porgy (Pagrus pagrus) fingerlings, respectively. In the first trial, six isoenergetic diets were formulated with protein levels ranging from 400 to 650 g kg−1 in increments of 50 g kg−1,...

The objective of this study was to determine the effect of two levels of inclusion of mannan oligosaccharides derived from the outer cell wall of a select strain of Saccharomyces cerevisiae (Bio-Mos, Alltech Inc, USA) on growth, feed utilization, immune status and disease resistance of European sea bass (Dicentrarchus labrax). Specimens of 35 g at...