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Mito-metagenomics (MMG) is becoming an alternative to amplicon metabarcoding for the assessment of biodiversity in complex biological samples using high-throughput sequencing. Whereas MMG overcomes the biases introduced by the PCR step in the generation of amplicons, it is not yet a technique free of shortcomings. First, as the reads are obtained f...
Amplicon metabarcoding is an established technique to analyse the taxonomic composition of communities of organisms using high-throughput DNA sequencing, but there are doubts about its ability to quantify the relative proportions of the species, as opposed to the species list. Here, we bypass the enrichment step and avoid the PCR-bias, by directly...
We provide new chorological, demographic, phenological, phytosociological, ecological and environmental management data of Simethis mattiazzi in Catalonia, a threatened vascular plant in the Mediterranean region. We have located 27 stands in the region of la Selva which counting give us an estimation of 176,052 individuals (22 stands). The area of...