Libero Gurrieri

Libero Gurrieri
University of Bologna | UNIBO · Department of Pharmacy and Biotechnology FaBiT

PhD
Working on redox regulation of Calvin cycle and starch enzymes, lignilolytic enzymes and microalgae for recycling.

About

18
Publications
3,391
Reads
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144
Citations
Introduction
Studying carbon metabolism and redox regulation: Calvin-Benson cycle, starch degradation, disulfide bonds, glutathionylation. Recently working on waste recycling by microalgae and plant biomass degradation by fungal enzymes.
Additional affiliations
April 2019 - present
University of Bologna
Position
  • PostDoc Position
Description
  • Working on plant/algae carbon metabolism (Calvin cycle, starch), biodiesel production from Chlorella strains, degradation for recycling of lignocellulosic wastes
May 2018 - May 2018
University of Bologna
Position
  • Students tutor for Practical Laboratory in Plant Biochemistry
November 2017 - February 2018
John Innes Centre
Position
  • PhD Student
Education
October 2013 - October 2015
University of Bologna
Field of study
  • Molecular and Cellular Biology
October 2009 - October 2013
University of Bologna
Field of study
  • Life Sciences

Publications

Publications (18)
Article
Arabidopsis (Arabidopsis thaliana) leaves possess a mechanism that couples the rate of night-time starch degradation to the anticipated time of dawn, thus preventing premature exhaustion of starch and night-time starvation. To shed light on the mechanism, we screened a mutagenized population of a starvation reporter line and isolated a mutant that...
Preprint
Oxygenic phototrophs perform carbon fixation through the Calvin–Benson cycle. Different mechanisms adjust the cycle and the light-harvesting reactions to rapid environmental changes. Photosynthetic glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a key enzyme of the cycle. In land plants, different photosynthetic GAPDHs exist: the most abundant...
Article
Thioredoxins (TRXs) are ubiquitous disulfide oxidoreductases structured according to a highly conserved fold. TRXs are involved in a myriad of different processes through a common chemical mechanism. Plant thioredoxins evolved into seven types with diverse subcellular localization and distinct protein targets selectivity. Five TRX types coexist in...
Article
Oxygenic phototrophs use the Calvin-Benson cycle to fix CO2 during photosynthesis. In the dark, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and phosphoribulokinase (PRK), two enzymes of the Calvin-Benson cycle, form an inactive complex with the regulatory protein CP12, mainly under the control of thioredoxins and pyridine nucleotides. In the l...
Article
Full-text available
Protein S-nitrosylation plays a fundamental role in cell signaling and nitrosoglutathione (GSNO) is considered as the main nitrosylating signaling molecule. Enzymatic systems controlling GSNO homeostasis are thus crucial to indirectly control the formation of protein S-nitrosothiols. GSNO reductase (GSNOR) is the key enzyme controlling GSNO levels...
Article
Full-text available
Culturing microalgae using dairy-wastes offers the opportunity of producing valuable biomass for different industrial applications. The capability of four Chlorella species and a recombinant Chlamydomonas reinhardtii strain to mixotrophically grow in wastewaters from an Italian dairy factory was investigated. A robust algal growth could be efficien...
Preprint
Full-text available
Thioredoxins (TRXs) are ubiquitous disulfide oxidoreductases structured according to a highly conserved fold. TRXs are involved in a myriad of different processes through a common chemical mechanism. Plant thioredoxins evolved into seven types with diverse subcellular localization and distinct protein targets selectivity. Five TRX types coexist in...
Article
Full-text available
Water shortage is an increasing problem affecting crop yield. Accumulation of compatible osmolytes is a typical plant response to overcome water stress. Sucrose synthase 1 (SUS1), and glucan, water dikinase 2 (GWD2) and δ1-pyrroline-5-carboxylate synthetase 1 (P5CS1) are members of small protein families whose role in the response of Arabidopsis th...
Preprint
Full-text available
Protein S-nitrosylation plays a fundamental role in cell signaling and nitrosoglutathione (GSNO) is considered as the main nitrosylating signaling molecule. Enzymatic systems controlling GSNO homeostasis are thus crucial to indirectly control the formation of protein S-nitrosothiols. GSNO reductase (GSNOR) is the key enzyme controlling GSNO levels...
Article
Full-text available
Reactive oxygen species (ROS) are produced in cells as normal cellular metabolic by-products. ROS concentration is normally low, but it increases under stress conditions. To stand ROS exposure, organisms evolved series of responsive mechanisms. One such mechanism is protein S-glutathionylation. S-glutathionylation is a post-translational modificati...
Article
In land plants and algae, the Calvin–Benson (CB) cycle takes place in the chloroplast, a specialized organelle in which photosynthesis occurs. Thioredoxins (TRXs) are small ubiquitous proteins, known to harmonize the two stages of photosynthesis through a thiol-based mechanism. Among the 11 enzymes of the CB cycle, the TRX target phosphoribulokinas...
Article
Full-text available
Thioredoxins (TRXs) are major protein disulfide reductases of the cell. Their redox activity relies on a conserved Trp-Cys-(Gly/Pro)-Pro-Cys active site bearing two cysteine (Cys) residues that can be found either as free thiols (reduced TRXs) or linked together by a disulfide bond (oxidized TRXs) during the catalytic cycle. Their reactivity is cru...
Article
Full-text available
Metabolism of starch is a major biological integrator of plant growth supporting nocturnal energy dynamics by transitory starch degradation as well as periods of dormancy, re-growth, and reproduction by utilization of storage starch. Especially, the extraordinarily well-tuned and coordinated rate of transient starch biosynthesis and degradation sug...
Preprint
Full-text available
In land plants and algae, the Calvin-Benson (CB) cycle takes place in the chloroplast, a specialized organelle in which photosynthesis occurs. Thioredoxins (TRXs) are small ubiquitous proteins, known to harmonize the two stages of photosynthesis through a thiol-based mechanism. Among the 11 enzymes of the CB cycle, the TRX target phosphoribulokinas...
Article
The genome of Arabidopsis thaliana encodes three glucan, water dikinases. Glucan, water dikinase 1 (GWD1; EC 2.7.9.4) and phosphoglucan, water dikinase (PWD; EC 2.7.9.5) are chloroplastic enzymes, while glucan, water dikinase 2 (GWD2) is cytosolic. Both GWDs and PWD catalyze the addition of phosphate groups to amylopectin chains at the surface of s...
Poster
The Calvin-Benson cycle is the most representative way to autotrophically process carbon dioxide. Several organisms, from prokaryotes to eukaryotes, adopted this cycle. However only two enzymes are unique of this metabolic pathway, Ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCo) and phosphoribulokinase (PRK). In the cycle, RuBisCo catalys...

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