Lian Chen

• Nanjing Forestry University

Pomacea canaliculata is recognized as a globally invasive aquatic species. Analyses of intestinal microbiota, dietary composition, and metabolism of invasive species can enhance our understanding of their feeding strategies and physiological adaptation strategies to the environment. Intestinal content samples were collected from P. canaliculata inh...

The gut microbiome is vital to the physiological and biochemical functions of the host, and changes in the composition of these microbial communities may affect growth and adaptability to the environment. Pomacea canaliculata is an invasive freshwater snail which has become a serious agricultural pest. Temperature adaptation is considered an import...

Gut microbiota is associated with host health and its environmental adaption, influenced by seasonal variation. Pomacea canaliculata is one of the world's 100 worst invasive alien species. Here, we used high‐throughput sequencing of the 16S rRNA gene to analyze the seasonal variation of gut microbiota of P. canaliculata. The results suggested that...

Background Tolerance of low temperature has a significant impact on survival and expansion of invasive snail Pomacea canalicuata . Cold acclimation can enhance cold tolerance of Pomacea canalicuata . To elucidate the molecular mechanism of P. canaliculata ’s responses to cold acclimation and cold stress, a high-throughput transcriptome analysis of...

Cold causes oxidative stress in living organisms, mainly caused by the accumulation of reactive oxygen species (ROS). In the antioxidant defense systems, Glutathione S-transferases (GSTs) play a vital role in the regulation of detoxification and redox balance of ROS. In this study, the P. canaliculata GST gene family were characterized using a geno...

Many-lined sun skink (Eutropis multifasciata) is a medium sized viviparous scincid lizard. Insufficient molecular markers have limited the effective conservation and management of E. multifasciata. In the present study, 28 novel single nucleotide polymorphism (SNP) markers were developed from the transcriptome dataset of E. multifasciata using SNaP...

Background The apple snail, Pomacea canaliculata, is one of the world’s 100 worst invasive alien species and vector of some pathogens relevant to human health. Methods On account of the importance of gut microbiota to the host animals, we compared the communities of the intestinal microbiota from P. canaliculata collected at different developmenta...

Characterizating the complete mitochondrial genome (mitogenome) of an organism allows detailed genomic studies in systematics and evolution. The present study decodes the mitogenome (17,062 bp) of the many-lined sun skink, Eutropis multifasciata, using next-generation sequencing. To compare the diversity of mitogenomic structure and investigate int...

The first complete mitochondrial genome sequence was determined for an oviparous lizard, Scincella modesta (Scincella, Scincidae). The total length of the complete mitochondrial genome was 17,511 bp, encodes 13 protein-coding genes, 22 tRNAs, 2 rRNA genes, and 2 non-coding regions. The overall base composition of S. modesta is A: 31.9%, T: 27.2%, G...

Procambarus clarkii was introduced into China as an important aquatic product in early 20th century. It has characteristics of high fertility, rapid growth, adaptability and digging burrows, which could cause damage of crops, cropland and facilities, decrease local biodiversity and thus threaten local ecosystem. Thus, predicting the potential distr...

Cathepsin L is one of the most important lysosomal cysteine proteases for the initiation of protein degradation, and it is involved in the immune response in many vertebrates. However, its function in the innate immune system of turtles remains poorly understood. Here, we cloned the cathepsin L gene from the Chinese soft‐shelled turtle Pelodiscus s...

A Caspase-14 gene homolog (designated as PsCaspase-14) in a reptile, the Chinese soft-shelled turtle Pelodiscus sinensis (Wiegmann, 1835), has been cloned and identified. The full-length cDNA of turtle PsCaspase-14 was consisted of 1,464 nucleotides with a 5’-terminal untranslated region (UTR) of 45 base pairs (bp), a 3’-terminal UTR of 198 bp with...

The golden apple snail, Pomacea canaliculata, is one of the world’s 100 most notorious invasive alien species. Knowledge about the critical climate variables that limit the global distribution range of the snail, as well as predictions of future species distributions under climate change, is very helpful for management of snail. In this study, the...

Understanding the spatial patterns in species richness is a central issue in macroecology and biogeography. Analyses that have traditionally focused on overall species richness limit the generality and depth of inference. Spatial patterns of species richness and the mechanisms that underpin them in China remain poorly documented. We created a datab...

Checklist of mammals based on habitat groups. (XLS)

Checklist of resident birds based on habitat groups. (XLS)

Residuals of species richness of mammals and resident birds and their habitat groups. (XLS)

Top six predictors selected based on hierarchical partitioning analysis. *P<0.05. (DOCX)

GLM multivariate models for the residuals of species richness of mammals and resident birds and their habitat groups. Six variables that explained most of the variance of the residuals of species richness were selected based on univariate regression models and hierarchical partitioning. The results of GLM multivariable regression based on six varia...

SLM multivariate models for species richness of mammals and birds and treating area as a variable in the models. Six variables that explained most of the variance of species richness were selected based on univariate regression models and hierarchical partitioning. We established the best multivariate model using GLM multivariable regression. To av...

Flow diagram of the statistical methods. (DOC)

Correlations between environmental variables. Number in the first row corresponds to the number of variables in the first column. 1-Mean annual precipitation. Spearman (two-sided) correlation was performed (n = 2376; **, P<0.01; *P<0.05). (DOC)

Variables selected based on Spearman (two-sided) correlation analysis and univariate regression for species richness of mammals and resident birds and their habitat groups. Spearman (two-sided) correlation analysis between any two variables in each category was performed. The deviance of each variable in each category explaining species richness wa...

Wetlands are under enormous pressure due to rapid economic development in China. Waterbirds, which depend on wetlands, are therefore facing a dramatic threat. Surveys were conducted in 19 Provinces in the winters of 2011/2012 and 2012/2013. Sites that support internationally important numbers of waterbirds were selected. Seven sites that support 20...

Abstract The mitochondrial genome of Pomacea canaliculata (Gastropoda: Ampullariidae) is the first complete mtDNA sequence reported in the genus Pomacea. The total length of mtDNA is 15,707 bp, which containing 13 protein-coding genes, 2 ribosomal RNAs, 22 transfer RNAs, and a 359 bp non-coding region. The A + T content of the overall base composit...

Invasive alien species (IAS) are a major global challenge requiring urgent action, and the Strategic Plan for Biodiversity (2011–2020) of the Convention on Biological Diversity (CBD) includes a target on the issue. Meeting the target requires an understanding of invasion patterns. However, national or regional analyses of invasions are limited to d...

Eleven polymorphic microsatellite loci are described for the multi-ocellated racerunner Eremias multiocellata (Squamata: Lacertidae). Loci were screened in 33 individuals of E. multiocellata. The number of alleles ranged from 11 to 17. The observed heterozygosity ranged from 0.515 to 0.939, and the expected heterozygosity from 0.875 to 0.925. These...

We report the characterization of 16 polymorphic microsatellite markers in the golden apple snail, Pomacea canaliculata, a pest registered in the list of "100 of the world's worst invasive alien species". The fast isolation by AFLP (Amplified Fragment Length Polymorphism) of sequences containing repeats (FIASCO) method was used to isolate microsate...