
Li WangTsinghua University | TH · Institute of Nuclear and New Energy Technology
Li Wang
Doctor of Chemistry
About
128
Publications
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Education
August 1999 - July 2004
September 1995 - July 1999
Publications
Publications (128)
Background
The screening biomarkers for early detection of colorectal cancer (CRC) is lacking. The aim is to identify epigenetic silenced genes and clarify its roles and underlying mechanism in CRC. We conducted integrative analyses of epigenome-wide Human Methylation 450 K arrays and transcriptome to screen out candidate epigenetic driver genes wi...
Radiation enteritis (RE) is a prevalent complication of radiotherapy for pelvic malignant tumors, characterized by severe intestinal epithelial destruction and progressive submucosal fibrosis. However, little is known about the pathogenesis of this disease and so far, there is no specific targeted therapy. Here, we report that CXCL16 is up‐regulate...
Accompanied by the adoption of aggressive cathodes to continuously improve batteries energy density, enhancing their safety is becoming increasingly urgent for the electric vehicle development. In-situ controllable formation of robust cathode-electrolyte interphase (CEI) with high inorganic content seems to be the most promising strategy to address...
Background:
Severe chronic radiation proctopathy (CRP) is difficult to treat.
Aim:
To evaluate the efficacy of colostomy and stoma reversal for CRP.
Methods:
To assess the efficacy of colostomy in CRP, patients with severe hemorrhagic CRP who underwent colostomy or conservative treatment were enrolled. Patients with tumor recurrence, rectal-va...
Objective:
The aim of this study was to evaluate the effect of the interval between CRT and surgery on radiation proctitis, the pathologic response, and postoperative morbidity.
Methods:
This was a cohort study from a phase III, randomized controlled trial (FOWARC study, NCT01211210). Data were retrieved from the leading center of the trial. Pat...
Background:
Neoadjuvant chemoradiotherapy (nCRT) is associated with post-operative anastomotic complications in rectal-cancer patients. Anastomosis involving at least one non-irradiated margin reportedly significantly reduces the risk of post-operative anastomotic complications in radiation enteritis. However, the exact scope of radiotherapy on th...
Background:
Prognostic assessment is crucial for optimal treatment. The aim of our study was to investigate the potential impact of estrogen receptor-α (ER-α) and progesterone receptor (PR) on the prognosis of colorectal cancer (CRC) patients who received curative resection.
Methods:
Retrospective evaluation of two independent cohorts of CRC pat...
Purpose:
In the multicenter, open-label, phase III FOWARC trial, modified infusional fluorouracil, leucovorin, and oxaliplatin (mFOLFOX6) plus radiotherapy resulted in a higher pathologic complete response rate than fluorouracil plus radiotherapy in Chinese patients with locally advanced rectal cancer. Here, we report the final results.
Methods:...
Background:
We demonstrated previously that radiation proctitis induced by preoperative radiotherapy is a predisposing factor for clinical anastomotic leakage in patients undergoing rectal cancer resection. Quantitative measurement of radiation proctitis is needed.
Objective:
This study aimed to quantitate the changes of anatomic features caused...
Tissue-derived RNA, DNA and protein samples become more and more crucial for molecular detection in clinical research, personalized and targeted cancer therapy. This study evaluated how to biobanking colorectal tissues through examining the influences of cold ischemic time and freeze-thaw cycles on RNA, DNA and protein integrity. Here, 144 pairs of...
Purpose: To determine whether there are differences in bone marrow tolerance to chemoradiotherapy (CRT) between two chemotherapy regimens according to FOWARC protocol and how chemotherapy regimens affect radiation dose parameters and normal tissue complication probability (NTCP) modelings that correlate with acute hematologic toxicity (HT) in recta...
Background:
Chronic radiation proctitis (CRP) is a complication which occurs in 1%-5% of patients who undergo radiotherapy for pelvic malignancies. Although a wide range of therapeutic modalities are available, there is no literature to date showing any particularly appropriate therapeutic modality for each disease stage. Argon plasma coagulation...
Background
Few studies on anastomotic condition after rectal-cancer resection and its effect on anastomotic leakage (AL) are available up to now. This study aimed to investigate potential radiation-induced injury left on surgical margins of anterior resection after neoadjuvant chemoradiotherapy (nCRT) and its association with AL.
Methods
We retros...
Background:
The self-expandable metal stent is used as a bridge to surgery in the treatment of acute malignant colorectal obstruction (AMCO). However, recent studies have shown inferior long-term outcomes and increased risk of tumor dissemination after stent placement. In addition, the optimal interval between stent placement and surgery is not cl...
Neoadjuvant chemoradiotherapy (nCRT) followed by surgery is the standard treatment for locally advanced rectal cancer. Here, we analyzed the impact of local and systemic environments on the tumor response to preoperative chemoradiotherapy in rectal cancer. We recruited 141 patients with rectal cancer treated with nCRT. We evaluated the local tumor...
Programmed death ligand-1 (PD-L1) is involved in inhibiting of T lymphocyte proliferation, producing cytokine, cytolytic activity, and suppressing of the immune response. Genes with molecular alterations involved in DNA mismatch repair promote cancer initiation and tumor progression. Clinical studies show that colorectal cancer (CRC) patients harbo...
This study aims to investigate the molecular characteristics of Chinese gastric cancer patients. In our study, the KRAS, BRAF, and PIK3CA mutation status of 485 GC patients were analyzed by Sanger sequencing. Kaplan-Meier analysis was used to plot survival curves according to different genotypes. The results show that the frequency of KRAS, BRAF an...
Background
Neoadjuvant radiation is recommended for locally advanced rectal cancer, with proven benefit in local control but not in disease-free survival. However, the impact of long-course radiation on postoperative bowel function and quality of life (QOL) remains controversial. This study aimed to investigate the impact of long-course neoadjuvant...
The incidence of colorectal cancer (CRC) is increasing in China. Here, we aimed to evaluate the latest demographic trends and KRAS/BRAF mutations status of Chinese CRC. Five thousand five hundred and forty‐six CRC patients diagnosed from 2010 to 2017 were involved. KRAS exon 2 and BRAFV600E mutations were detected by Sanger sequencing and high‐reso...
Objectives
To develop and validate radiomic models in evaluating biological characteristics of rectal cancer based on multiparametric magnetic resonance imaging (MP-MRI).
Methods
This study consisted of 345 patients with rectal cancer who underwent MP-MRI. We focused on evaluating five postoperative confirmed characteristics: lymph node (LN) metas...
Objective:
The aim of this study is to introduce a new type of cross suture in closing peritoneal incision after abdominoperineal resection (APR) for rectal cancer.
Methods:
This new type of cross suture was firstly proposed and applied in a small cohort in our hospital. In this study, we reported its efficacy and safety. From Feb 2018 to May 20...
Municipal solid waste incinerator (MSWI) fly ash contains large amounts of arsenic and antimony. This study focuses on the incorporation and evaporation of arsenic and antimony when MSWI fly ash added to clinker raw meal. Thermal analysis was used to simulate the “single incorporation” of arsenic and antimony into clinker with HSC. Statistical anal...
Current practice of neoadjuvant therapy and total mesorectal excision (TME) in rectal cancer bears the weakness in systemic disease control and long-term functional outcomes. With increasing concerns of the balance between cure and quality of life, new strategies are developed to better oncological outcomes at least cost of function damage. Attract...
Chronic radiation proctopathy (CRP) is the delayed adverse effect after radiotherapy for pelvic malignancies. It is characterized as progressive fibrosis in pathology. The symptoms are usually unspecific which include rectal bleeding, perianal pain, tenesmus, stool frequencies and so on. The course of CRP can present from months to years after radi...
Background:
Chronic radiation proctitis (CRP), a common complication after radiotherapy for pelvic malignancies, compromises patient quality of life. Vascular damage and aberrant angiogenesis in the mucosal layer are essential histological features, but changes to the submucosal layer are unclear. Thus, we evaluated the histological characteristic...
Background and objective:
With the increase in sphincter preserving rate of rectal cancer (RC) cancer, postoperative quality-of-life, such as genital dysfunction, has become a major issue in the patient management. In this study, we proposed a measurement, namely, the sphincter preserving length (SPL), and investigated the relationship between SPL...
Aim:
To investigate the relationship between mutations of key genes in the EGFR signaling pathway and the prognosis of stage II colorectal cancer patients without chemotherapy.
Materials & methods:
The incidence of KRAS, NRAS, BRAF, PIK3CA mutations and deficient DNA mismatch repair were assessed in 160 stage II colorectal cancer patients who ha...
Fig. S1. Rho GDP‐dissociation inhibitor α (RhoGDIα) depletion represses colorectal cancer cell proliferation and migration. (a) Quantitative real‐time PCR was used to assess RhoGDIα knockdown efficiency in HCT116 cells. Error bars indicate SD (n = 3). ***P < 0.001. (b–d) Colorectal cancer cells were transfected with siControl or siRhoGDIα‐2 oligos....
Fig. S2. Rho GDP‐dissociation inhibitor α (RhoGDIα) induces telomeric repeat factor 1 (TRF1) expression through the phosphatidylinositol 3‐kinase–protein kinase B (PI3K–Akt) signaling pathway. (a) Quantitative real‐time PCR for the expression of six shelterin/telosome proteins in HCT116 cells expressing GFP or RhoGDIα. Error bars indicate SD (n = 3...
Fig. S3. Receiver–operating characteristic curves to determine cut‐off scores for Rho GDP‐dissociation inhibitor α (RhoGDIα) and telomeric repeat factor 1 (TRF1) expression in patients with colorectal cancer.
The aim of this study was to summarize the imaging features of chronic radiation proctitis (CRP) on endorectal ultrasound (ERUS) and investigate the value of ERUS in the evaluation of disease activity. 40 CRP patients and 30 control patients were investigated by ERUS. Rectal wall thickness and layers, ulcers and rectovaginal fistulas were evaluated...
RhoGDIα is an essential regulator for Rho GTPases. Although RhoGDIα may serve as an oncogene in colorectal cancer (CRC), the underlying mechanism is still unclear. Here,we investigate the function, mechanism, and clinical significance of RhoGDIα in CRC progression. We founded that downregulation of RhoGDIα repressed CRC cell proliferation, motility...
The currently known Mendelian colorectal cancer (CRC) predisposition syndromes account for ∼5–10% of all CRC cases, and are caused by inherited germline mutations in single CRC predisposing genes. Using molecular inversion probes (MIPs), we designed a targeted next-generation sequencing panel to identify mutations in seven CRC predisposing genes: A...
Background
Radiation-induced rectovaginal fistula (RVF) is a severe and difficult complication after pelvic malignancy radiation. This study was to retrospectively compare the outcomes of restorative resection and colostomy only in remission of anorectal symptoms. Methods
We enrolled a cohort of 26 consecutive cases who developed RVF after pelvic r...
Background/aims:
Mitogen- and stress-activated protein kinase 1 (MSK1) has recently been implicated in cell proliferation and neoplastic transformation. However, the involvement of MSK1 in colorectal cancer (CRC) has not been addressed. This study aimed to evaluate the expression and potential functions of MSK1 in CRC.
Methods:
The MSK1 expressi...
TPS816
Background: Preoperative 5-Fluorouracil based chemoradiotherapy is the standard of treatment for locally advanced rectal cancer. About 15% to 18% of patients would achieve pathologic complete response (pCR) after 5-Fluorouracil based chemoradiation. And the survival outcome of patients with pCR was much better than that of non-pCR. In our pr...
Cytoplasmic polyadenylation element-binding protein 4 (CPEB4), a zinc-finger-containing sequence-specific RNA-binding protein, has been associated with tumor proliferation, invasion, and migration. Expression of CPEB4 and its prognostic significance in human colorectal cancer (CRC) have not been studied. The aim of the present study was to investig...
Background: The case–control study aimed to investigate the association between the −31G>C polymorphism in the promoter of survivin gene and the susceptibility to sporadic colorectal cancer (CRC) in a Southern Chinese population.
Materials and Methods: The study was carried out on 711 healthy controls and 702 CRC cases of a Southern Chinese popula...
Titania nanotube arrays (TNAs) were grown by anodic oxidation method, and molybdenum disulfide (MoS2) grafted TNAs have been synthesized via one-step hydrothermal process. The MoS2 grafted TNAs (MoS2/TNAs) when employed as an anode material in lithium ion battery, exhibited excellent areal specific capacity (~430 µAh cm⁻²) at current density of 50...
Background:
Evidence regarding the effect of preoperative radiotherapy on anastomotic integrity remains conflicting in rectal cancer surgery. Prospective comparisons with appropriate controls are needed.
Objective:
This study aimed to assess the impact of preoperative radiotherapy on anastomotic leakage and stenosis after rectal cancer resection...
5-fluorouracil (5-FU)-based chemotherapy is the main chemotherapeutic approach for colorectal cancer (CRC) treatment. Because chemoresistance occurs frequently and significantly limits CRC therapies, a novel agent is needed. Pseudolaric acid B (PAB), a small molecule derived from the Chinese medicinal herb ‘‘Tujinpi’’, exhibits strong cytotoxic eff...
This study was to investigate whether the time to the lowest postoperative CEA can predict cancer survival. We enrolled 155 rectal cancer patients in this retrospective and longitudinal cohort study. Deepness of response (DpR) of CEA refers to the relative change of the lowest postoperative CEA level from baseline, and time to DpR (TTDpR) refers to...
Spleen tyrosine kinase (SYK) has been reported as a potential tumor suppressor in colorectal cancer (CRC). However, the role of alternative splicing of SYK in carcinogenesis remains unclear. In the present study, SYK isoforms were overexpressed in the human CRC HCT 116 cell line using lentiviral expression vectors to investigate the biological func...
Objective
The aim was to evaluate erectile and urinary function of male patients with rectal cancer treated by neoadjuvant (NA) chemoradiotherapy (CRT) or NA chemotherapy only.
Methods
In this prospective randomized trial (ClinicalTrials.gov NCT01211210; “FOWARC”), we included 102 men who received NA therapy for stage II–III rectal cancer between J...
Aim:
To assess the efficacy and safety of diverting colostomy in treating severe hemorrhagic chronic radiation proctitis (CRP).
Methods:
Patients with severe hemorrhagic CRP who were admitted from 2008 to 2014 were enrolled into this study. All CRP patients were diagnosed by a combination of pelvic radiation history, clinical rectal bleeding, an...
Municipal solid waste incinerator (MSWI) fly ashes are characterized by high calcium oxide (CaO) content. Carbon dioxide (CO2) adsorption by MSWI fly ash was discussed based on thermogravimetry (TG)/differential thermal analysis (DTA), minerology analysis, and adapting the Stenoir equation. TG/DTA analysis showed that the weight gain of the fly ash...
Background
Mitogen/extracellular signal-regulated kinase kinase-5 (MEK5) has been confirmed to play a pivotal role in tumor carcinogenesis and progression. However, few studies have investigated the role of MEK5 in colorectal cancer (CRC).
Methods
MEK5 expression was determined by immunohistochemistry (IHC) in tissue microarrays (TMAs) containing...
Appropriate management and treatment of fly ash from municipal solid waste (MSW) incineration plant have become an urgent environmental protection problem. In this study, glass–ceramics have been prepared from MSW incineration fly ash and the SiO2 with different blending ratios ((Formula presented.)). With the increase in (Formula presented.), the...
The behavior of single bubble rising in quiescent shear-thinning fluids was investigated numerically by level set method. A number of bubbles in a large range of Reynolds number and Eotvos number were investigated including spherical, oblate and spherical. The bubble shape and drag coefficient were compared with experimental results. It is observed...
Secondary batteries play important roles in energy storage. Secondary batteries such as lithium/sodium-ion batteries are promising for portable electronic devices, electric vehicles and green energy storage. The research on novel electrode materials is important for rechargeable battery of next generation. Phosphorus is attractive as anode material...
Objective:
To establish the classification of colorectal cancer recurrence patterns based on their relations to prognosis and recurrence.
Methods:
A total of 463 patients with recurrent rectal cancer (Stage I-III) following curative resection in our hospital from June 2002 to June 2012 were included consecutively. Recurrence status, time, locati...
MSWI fly ash is a potential substitute for some virgin materials, but the soluble salts and hazardous trace elements in the ash can limit this potential. This study investigated the use of a water-based washing process to remove the soluble salts from MSWI fly ash. The removal of trace elements by bubbling CO2 through the resulting wastewater was a...
Effects of chlorine and phosphor on the transformation of zinc during combustion of sewage sludge (SS) was investigated by two approaches: combustion experiment and thermodynamic simulation. The result of thermodynamic simulation predicted that zinc existed as ZnSO4 (s) below 400°C, while mainly as phosphate in solid phase when the combustion tempe...
Objective:
To develop an accurate model with pre-treatment parameters to predict tumor regression and down-staging in locally advanced rectal cancer patients, basing the cohort of preoperative chemotherapy alone in FOWARC study.
Patients and methods:
From Jan 2011 to Feb 2015, complete data was available for 137 out of 165 patients who received...
The possibility of producing calcium sulphoaluminate cement (CSA) by adding municipal solid waste incinerator (MSWI) fly ash to raw meal was investigated. After subjecting MSWI fly ash to accelerated carbonation and washing with water (ACW), various amounts (i.e. 5, 10 and 15 wt%) of the treated ash were added to raw meal composed of a mixture of b...
Colorectal liver metastases (CLM) occur frequently and postoperative intestinal infection is a common complication. Our previous study showed that probiotics could decrease the rate of infectious complications after colectomy for colorectal cancer. To determine the effects of the perioperative administration of probiotics on serum zonulin levels wh...
Background:
The purpose of this study was to develop an in situ immune marker model to predict postoperative oncological outcomes in patients with colorectal cancer (CRC).
Methods:
Immunohistochemistry for 13 immune cell markers was performed on tumor tissue microarrays from 300 CRC patients who underwent curative resection from January 2000 to...
Purpose: To evaluate retrospectively the postoperative genital function, the local recurrence, and the survival rate impacted by laparoscopic or open surgery for rectal cancer (RC) in male patients. Methods: A total of 398 male RC patients after laparoscopic or open total mesorectal excision (TME) of rectomy (205 patients in the TME with laparoscop...
Lymph node metastasis is an important factor determining the outcome of colorectal cancer. Although epithelial-to-mesenchymal transition (EMT), TNF-α and microRNA (miRNA) have been found to play important roles in lymph node metastasis, the underlying molecular mechanism remains unclear. Here we reported that high expression of microRNA-19a (miR-19...
Biomarkers for predicting prognosis are critical to treating colorectal cancer (CRC) patients. We found that CSN6, a subunit of COP9 signalosome, is overexpressed in CRC samples and that CSN6 overexpression is correlated with poor patient survival. Mechanistic studies revealed that CSN6 is deregulated by epidermal growth factor receptor (EGFR) sign...
Long non-coding RNA, urothelial cancer associated 1 (UCA1), is reported to play a critical role in progression of carcinogenesis. In the present study, we identified differential expression of UCA1 in colorectal cancer (CRC) and paired peritumoral tissues using gene expression microarray analyses. qPCR analysis confirmed that UCA1 was upregulated i...