Li-Dong Wang

• Zhengzhou University

Graphene-copper (Gr-Cu) composites exhibit significant potential for industrial applications. Among the methods for fabricating Gr-Cu composites, the in-situ growth method stands out as a simple yet effective approach. However, graphene converted from liquid or solid molecules by the traditional in-situ growth method often exhibits numerous defects...

Background Gastric cardia adenocarcinoma (GCA) is classified as Siewert type II adenocarcinoma at the esophagogastric junction in Western countries. The majority of GCA patients do not exhibit early warning symptoms, leading to over 90% of diagnoses at an advanced stage, resulting in a grim prognosis, with less than a 20% 5‐year survival rate. Met...

Endoscopic biopsy and histopathological examination remain one of the critical methods for high-risk subjects (HRS) screening for esophageal squamous cell carcinoma (ESCC) in symptom-free subjects (SFS) of high-incidence areas (HIA) for ESCC. Almost 90% of the symptom-free residents show normal esophageal epithelia in HIA of ESCC. Based on that, ov...

The aim of this work is to analyze the clinicopathological characteristics and prognostic factors of patients with nuclear pedigree of esophageal cancer. The clinicopathological data and follow-up information of 3,260 patients from different nuclear pedigree of esophageal cancer who underwent radical resection of esophageal cancer were collected, a...

Background The impact of hospital volume on the long-term survival of esophageal squamous cell carcinoma (ESCC) has not been well assessed in China, especially for stage I–III stage ESCC. We performed a large sample size study to assess the relationships between hospital volume and the effectiveness of ESCC treatment and the hospital volume value a...

Background This study was intended to construct a brand new prognostic nomogram after combine clinical and pathological characteristics to increases prognostic value in patients with esophageal squamous cell carcinoma. Methods A total of 1,634 patients were included. Subsequently, the tumor tissues of all patients were prepared into tissue microar...

Keratin pearls (KP) is an important indicator of the degree of tumor cell differentiation of esophageal squamous cell carcinomas (ESCC). However, the independent prognostic value of KP in ESCC patients remains unclear. The hematoxylin-eosin (H&E) stained tissue microarrays (TMAs) or whole slides of the patients were prepared to identify the existen...

Purpose Some studies indicated that gender is associated with prognostic of cancer, However, currently the prognostic value of gender for gastric cardia adenocarcinoma (GCA) survival is unclear. The aim of our study is to reveal the influence of gender on the prognosis of patients with GCA. Patients and methods A total of 42,345 cases Chinese GCA...

Background: Primary malignant melanoma of the esophagus (PMME) is a rare malignant disease and has not been well characterized in terms of clinicopathology and survival. Aim: To investigate the clinical features and survival factors in Chinese patients with PMME. Methods: The clinicopathological findings of ten cases with PMME treated at Henan...

Background This study compared the survival outcomes of different surgical approaches to determine the optimal approach for gastric cardia adenocarcinoma (GCA) and aimed to standardize the surgical treatment guidelines for GCA. Methods A total of 7103 patients with GCA were enrolled from our previously established gastric cardia and esophageal car...

While genetic alterations in several regulators of the cell cycle have a significant impact on the gastric carcinogenesis process, the prognostic role of them remains to be further elucidated. The TCGA-STAD training set were downloaded and the mRNA expression matrix of cell cycle genes was extracted and corrected for further analysis after taking t...

Background: In China, it has been well recognized that some female patients with esophageal squamous cell carcinoma (ESCC) have different overall survival (OS) time, even with the same tumor-node-metastasis (TNM) stage, challenging the prognostic value of the TNM system alone. An effective predictive model is needed to accurately evaluate the prog...

Graphene/Cu composite wire with a diameter as fine as 195 μm was successfully fabricated for the first time by using a synergistic methodology consists of in-situ growth high-quality graphene, spark plasma sintering, hot-extrusion and drawing. The graphene/Cu composite with a continuous severe drawing deformation of 99.7% was easily achieved, which...

Esophageal squamous cell carcinoma (ESCC) is one of the most common aggressive malignancies worldwide, particularly in northern China. The absence of specific early symptoms and biomarkers leads to late-stage diagnosis, while early diagnosis and risk stratification are crucial for improving overall prognosis. We performed UPLC-MS/MS on 450 ESCC pat...

Background: Gastric cardia adenocarcinoma (GCA), which has been classified as type II adenocarcinoma of the esophagogastric junction in western countries, is of similar geographic distribution with esophageal squamous cell carcinoma in China, and even referred as "sister cancer" by Chinese oncologists. The molecular mechanism for GCA is largely un...

The role of focal amplifications and extrachromosomal DNA (ecDNA) is unknown in gastric cardia adenocarcinoma (GCA). Here, we identify frequent focal amplifications and ecDNAs in Chinese GCA patient samples, and find focal amplifications in the GCA cohort are associated with the chromothripsis process and may be induced by accumulated DNA damage du...

Background: Primary small cell carcinoma of the esophagus (PSCE) is a highly invasive malignant tumor with a poor prognosis compared with esophageal squamous cell carcinoma. Due to the limited samples size and the short follow-up time, there are few reports on elucidating the prognosis of PSCE, especially on the establishment and validation of a s...

Riboflavin is an essential micronutrient for normal cellular growth and function. Lack of dietary riboflavin is associated with an increased risk for esophageal squamous cell carcinoma (ESCC). Previous studies have identified that the human riboflavin transporter SLC52A3a isoform (encoded by SLC52A3) plays a prominent role in esophageal cancer cell...

Extrachromosomal DNA plays an important role in oncogene amplification in tumour cells and poor outcomes across multiple cancers. However, the function of extrachromosomal DNA in gastric cardia adenocarcinoma (GCA) is very limited. Here, we investigated the availability and function of extrachromosomal DNA in GCA from a Chinese cohort of GCA using...

Background: Preoperative pulmonary function plays an important role in selecting surgical candidates and assessing postoperative complications. Reduced pulmonary function is associated with poor survival in several cancers, but the prognostic value of preoperative pulmonary function in esophageal squamous cell carcinoma (ESCC) is unclear. Nutritio...

Hot rolling is one of the most important thermoforming methods for metal matrix composites. However, to date, there are limited studies devoted to the research on the hot rolling behavior of graphene/metal composites. In this work, the influence of hot rolling on the evolution of microstructures, textures, and tensile properties of the graphene/Cu...

Esophageal squamous cell carcinoma (ESCC) occurs with highest frequency in China with over 90% mortality, highlighting the need for early detection and improved treatment strategies. We aimed to identify ESCC cancer predisposition gene(s). Our study included 4,517 individuals. The discovery phase using whole‐exome sequencing (WES) included 186 fami...

A low-cost and easily-accessible approach based on adsorption of small aromatic hydrocarbons (SAHs) was developed to synthesize graphene on Cu powders, which could supply raw material for powder metallurgy (PM) fabrication of graphene/Cu composites. Two common SAHs, naphthalene and α-naphthol, were chosen as the carbon precursors. Compared with nap...

Purpose: The genetic basis of esophageal squamous cell carcinoma (ESCC) still remains unclear. Several genome-wide association studies identified some common variants associated with small effects. Here we aimed to identify some rare deleterious variants in cancer predisposition genes, which may contribute to the genetic susceptibility of ESCC with...

Introduction: Esophageal squamous cell carcinoma (ESCC) has an especially high incidence in Northern China, where there is evidence for a significant familial association. We performed targeted next-generation sequencing (NGS) analysis on familial ESCC germline samples compared to non-cancer controls from the same high-risk region and compiled a li...

Conventional ferroelectric oxides are of generally non-centrosymmetric crystals resulting from paraelectric-ferroelectric transitions. Here, we present a new donor-acceptor ion co-doping route to room-temperature ferroelectricity in rutile TiO2, where ionic pair of co-doping ions plays very important roles in generating local broken symmetry and po...

Based on our initial genome-wide association study (GWAS) on esophageal squamous cell carcinoma (ESCC) in Han Chinese, we conducted a follow-up study to examine the single nucleotide polymorphisms (SNPs) associated with family history (FH) of upper gastrointestinal cancer (UGI) cancer in cases with ESCC. We evaluated the association between SNPs an...

The rarity of primary small cell carcinoma of the esophagus (PSCE) has limited the clinical feature and survival analysis with large sample size. Tissue chromogranin A (CgA) protein expression has been reported to be a useful biomarker for diagnosing PSCE. Interestingly, recent studies have indicated tissue CgA as a significant prognostic marker in...

Background Our previous genome-wide association study (GWAS) identified three independent single nucleotide polymorphisms (SNPs) in human major histocompatibility complex (MHC) region showing association with esophageal squamous cell carcinoma (ESCC). In this study, we increased GWAS sample size on MHC region and performed validation in an independ...

STROBE_checklist_v4_combined_PlosMedicine. File contains the STROBE Checklist of this paper. (DOCX)

Genome-wide association studies (GWAS) of gastric cancer have reported differences in single-nucleotide polymorphism (SNP) associations for tumour subtypes, particularly when divided by location into the gastric cardia versus the non-cardia. Here we present results for a GWAS using 2350 East Asian gastric cancer cases divided as 1189 gastric cardia...

This study sheds light on the effect of alumina on dielectric constant and dielectric loss of novel CaCu3Ti4O12 composite ceramics. Alumina, at several concentrations, was deposited on the surface of CaCu3Ti4O12 particles via sol-gel technique. The dielectric constant significantly increased for all frequencies and the dielectric loss substantially...

Proceedings: AACR Annual Meeting 2014; April 5-9, 2014; San Diego, CA We conducted a joint (pooled) analysis of three genome-wide association studies (GWAS) of esophageal squamous cell carcinoma (ESCC) in ethnic Chinese (5,337 ESCC cases and 5,787 controls) with 9,654 ESCC cases and 10,058 controls for follow-up. In a logistic regression model adj...

CaCu3Ti4O12 powder was prepared by sol-gel technique, and the CaCu3Ti4O12 composites were sintered at 1 000°C for different time after cold pressing with the same pressure and time. The results exhibit that the grain size is affected by the pressure and sintering time. The impedance spectrum measurement results show that both the dielectric constan...

Worldwide, the highest prevalence of esophageal cancer (EC) occurs in Northern China. High-density SNP arrays allow identification of identity-by-descent (IBD) segments in genomic DNAs representative of shared common ancestral regions. We utilized IBD approaches to map susceptibility loci associated with low-penetrance SNPs in high-risk Henan hered...

To evaluate the diagnostic values of using autoantibodies in sera to a panel of eight tumor‐associated antigens (TAAs) of P53, Koc, P62, C‐myc, IMP1, Survivn, P16 and Cyclin B1 full‐length recombinant proteins for early detection of patients with gastric cardia adenocarcinoma (GCA) and high‐risk subjects screening. Enzyme‐linked immunosorbent assay...

BACKGROUND:The human major histocompatibility complex (MHC) is the most important region in vertebrate genome, and is crucial in innate immunity. Recent studies have demonstrated the possible role of polymorphisms in the MHC region to high risk for esophageal squamous cell carcinoma (ESCC). Our previous genome-wide association study (GWAS) has indi...

The etiological role of human papillomavirus (HPV) in cervical cancer has been well established. However, it is inconclusive whether HPV plays the same role in esophageal carcinogenesis. In this study, we detected HPV infection in 145 frozen esophageal tissues, including 30 normal epithelium (ENOR), 37 dysplasia (DYS) and 78 invasive squamous cell...

A single-nucleotide polymorphism (rs2274223: A5780G:His1927Arg) in the phospholipase C epsilon gene (PLCε) was recently identified as a susceptibility locus for esophageal cancer in Chinese subjects. To determine the underlying mechanisms of PLCε and this SNP in esophageal carcinogenesis, we analyzed PLCε genotypes, expression, and their correlatio...

The aim of this study was to evaluate the diagnostic values by detecting sera autoantibodies to eight tumor-associated antigens (TAAs) of P53, IMP1, P16, cyclin B1, P62, C-myc, Survivn and Koc full-length recombinant proteins for the screening of high-risk subjects and early detection of esophageal squamous cell carcinoma (ESCC). Enzyme-linked immu...

Cytochrome P4501A1 (CYP1A1) enzyme is a member of the CYP superfamily of enzymes. CYP1A1 A2455G and T3801C are two most commonly studied polymorphisms loci. Previous studies have reported that CYP1A1 polymorphisms increase esophageal cancer (EC) risk. However, the results remain controversial and ambiguous. To further investigate the association be...

The aim of this study was to characterize histo-chemical patterns and Das-1 and Ki67 protein expression in gastric cardia adenocarcinoma (GCA) and intestinal metaplasia (IM) lesions adjacent to GCA. Histochemical techniques, including Alcian blue/periodic acid-Schiff (AB/PAS), high iron diamine/Alcian blue (HID/AB) and avidin-biotin-peroxidase comp...

Background The role of tumor suppressor gene RASSF1A in the esophageal and gastric cardia carcinogenesis is still inconclusive. In this study, the polymorphism, promoter methylation and gene expression of RASSF1A were characterized in esophageal squamous cell carcinoma (ESCC) and gastric cardia adenocarcinoma (GCA). Methods We firstly analyzed the...

An investigation was conducted in a 2.55 hm2 plot of Pinus koraiensis-dominated broad-leaved mixed forest to study the microsite characteristics of pit and mound formed by 42 treefalls and the status of vegetation regeneration on the microsites. The soil water content, soil temperature, relative air humidity, and photosynthetically active radiation...

In order to explore the relationship between human papilloma virus ( HPV) and upper gastrointestinal cancer(esophageal cancer), An esophageal squamous cell carcinoma(ESCC) tissue was obtained from a 76 year old Chinese female patient from Anyang city, a high-incidence area for esophageal cancer, in China. Transplanted tumor was formed through direc...

Objective: Biomarker assay is a noninvasive method for the early detection of esophageal squamous cell carcinoma (ESCC). Searching for new biomarkers with high specificity and sensitivity is very important for the early detection of ESCC. Serum surface-enhanced laser desorption/ionization-time of flight mass spectrometry (SELDI-TOF-MS) is a high t...

Objective: To explore the association of C20orf54 gene rs3746804 position single nucleotide polymorphism and susceptibility to esophageal squamous cell carcinoma (ESCC). Methods: Purification of genomic DNA from whole blood was used the Maxwell(16) System. rs3746804 in C20orf54 was detected by direct sequencing in 434 ESCC patients from Changzhi...

Genomic instability caused by telomere erosion is an important mechanism of tumorigenesis. p53 plays a key role in cellular senescence and/or apoptosis associated with telomere erosion which positions p53 as a guard against tumorigenesis. The present study was undertaken to investigate the potential interactions between p53 functional mutations, po...

Esophageal cancer ranks sixth in cancer death. To explore its genetic origins, we conducted exomic sequencing on 11 esophageal adenocarcinomas (EAC) and 12 esophageal squamous cell carcinomas (ESCC) from the United States. Interestingly, inactivating mutations of NOTCH1 were identified in 21% of ESCCs but not in EACs. There was a substantial dispar...

Proceedings: AACR 103rd Annual Meeting 2012‐‐ Mar 31‐Apr 4, 2012; Chicago, IL Lymph node (LN) metastasis has been recognized as one of the most important factors for poor prognosis of esophageal cancer (EC). However, the influence of dissected LN number on metastatic detection rate and survival in EC has not been well documented. The present study...

Genome-wide association studies have identified susceptibility loci for esophageal squamous cell carcinoma (ESCC). We conducted a meta-analysis of all single-nucleotide polymorphisms (SNPs) that showed nominally significant P-values in two previously published genome-wide scans that included a total of 2961 ESCC cases and 3400 controls. The meta-an...

Comparative and statistical analysis the HPV infection rate between fresh tissue and Paraffin-embedded Specimens of esophageal squamous cell carcinoma,and comparative the testing results with others regions. Extracted the total DNA from the novel fresh tissue and Paraffin-embedded Specimens; Detected the DNA by PCR with universal primer and Detecte...

To determine whether CDX2 and villin protein expression are associated with intestinal metaplasia (IM) in gastric cardiac mucosa and to explore the relationship with evolution of gastric cardiac adenocarcinoma (GCA). We studied 143 gastric cardiac biopsy or resection specimens from Henan province China, including 25 cardiac gastritis specimens with...

To evaluate the effects and safety of combination chemotherapy with oxaliplatin (L-OHP) and S-1 (SOX regimen) in older patients with advanced gastric cardiac adenocarcinoma (GCA). Seventy patients with advanced GCA were classified according to age into an older group (≥ 75 years) and a control group (< 75 years). The SOX regimen was administered to...

To study the relationship between plasma riboflavin levels and esophageal squamous cell carcinoma. We detected and compared plasma concentrations of riboflavin in patients with esophageal squamous cell carcinoma (ESCC) and immigrants of Linzhou living in Changzhi. Plasma riboflavin levels were quantified in 445 ESCC patients, 689 healthy control su...

In accordance with the hypothesis that cancer formation is a process of somatic evolution driven by natural selection, signature of positive selection has been detected on a number of cancer-related nuclear genes. It remains, however, controversial whether a similar selective pressure has also acted on mitochondrial DNA (mtDNA), a small molecule in...

The group 2 LIM domain protein, Cysteine-rich intestinal protein 2 (CRIP2) was found to play an important role in esophageal squamous cell carcinoma (ESCC) tumorigenesis. Subcellular fractionation studies show that CRIP2 is expressed in the nucleus. Real-time quantitative PCR shows CRIP2 expression is down-regulated in ESCC tissues and cell lines....

β-LiAlSiO4 reinforced Cu matrix composites (Euc/Cu) were fabricated by hot-pressed sintering process. Thermal expansion behaviors of Euc/Cu composites were studied during the thermal cycling process. Upon twice thermal cycle, the temperature dependence of the coefficients of thermal expansion (CTE) for Euc/Cu composites tends to be stable. The CTEs...

To study the promoter methylation pattern of p16 and hMLH1 genes in esophageal squamous cell carcinoma and reflux esophagitis, and to correlate the results with clinical and pathologic findings. Twelve cases of normal esophagus, 13 cases of esophageal squamous cell carcinoma, 43 cases of reflux esophagitis with basal cell hyperplasia and 21 cases o...

To evaluate the association between xeroderma pigmentosum group D (XPD), genetic polymorphism Lys751Gln and esophageal cancer risk. We searched PubMed up to September 1, 2010 to identify eligible studies. A total of 10 case-control studies including 2288 cases and 4096 controls were included in the meta-analysis. Statistical analysis was performed...

The effect of interface reaction on the fractal dimensions of fracture surface in aluminum borate whisker-reinforced aluminum alloy 6061 composite was investigated. The composite was fabricated by squeeze-casting technique. The fracture surfaces created in tensile test were measured by vertical sectioning method. Fractal phenomena were found in two...

Oesophageal squamous cell carcinoma (SCC) causes the highest number of cancer deaths in some regions of Northern China. Previously, we narrowed down a critical region at 9q33-34, identified to be associated with tumour-suppressive function of deleted in oesophageal cancer 1 (DEC1) in oesophageal SCC. We generated DEC1 antibody and constructed tissu...

To investigate the prevalence of human papillomavirus (HPV), particularly of high-risk HPV in biopsy tissue specimens of esophageal carcinomas in Linzhou city. General nested primer sets were used to detected the whole HPV genotypes, following by HPV16 and 18 type specific PCR for the HPV16 and 18 detection respectively. All 18 biopsy samples were...

C20orf54, also known as a human riboflavin transporter 2 (RFT2), encodes an open reading frame protein RFT2 newly identified to play an important role in esophageal carcinogenesis by modulating riboflavin uptake. Missense cSNPs on exon 3,1172 C>A (T391M) and 1246A>G (I416V) have been suggested to modulate protein expression. The aim of present stud...

To investigate the relationship between human papillomavirus (HPV) infection and concurrent esophagus and gastric cardia cancer from the same patient (CC) and examine the significance of P16(INK4A) protein expression. Polymerase chain reaction was used to detect the presence of HPV type16 (HPV16). The expression of P16(INK4A) protein was detected u...

We performed a genome-wide association study of esophageal squamous cell carcinoma (ESCC) by genotyping 1,077 individuals with ESCC and 1,733 control subjects of Chinese Han descent. We selected 18 promising SNPs for replication in an additional 7,673 cases of ESCC and 11,013 control subjects of Chinese Han descent and 303 cases of ESCC and 537 con...

Esophageal cancer (EC) is one of the six most common cancers across the world. The striking 3-4: 1male predominance of esophageal squamous cell carcinoma (ESCC) has not yet been well explained. Our hypothesis is that the changes in level of estrogen and/or subtype of estrogen receptor (ER) may exert a protective factor in esophageal carcinogenesis...

Esophageal carcinogenesis is a multi-stage process, involving a variety of changes in gene expression and physiological structure change. MicroRNAs (miRNAs) are a class of small non-coding endogenous RNA molecules. Recent innovation in miRNAs profiling technology have shed new light on the pathology of esophageal carcinoma (EC), and also heralded g...

The striking 3-4:1 male predominance of esophageal squamous cell carcinoma (ESCC) has not yet been well explained. Our hypothesis is that the changes in level of estrogen and/or subtype of estrogen receptor (ER) may exert a protective factor in esophageal carcinogenesis and prognosis of ESCC. Radioimmunoassay (RIA) was used to determine the serum l...

Background and objective: The mechanism of tumor tissues selectively uptake the photosensitizer in photodynamic therapy (PDT) is still unclear. This study was to investigate the affinity of tumor cells to the photosensitizer photofrin-II. Methods: Ultraviolet spectrophotometer was applied to measure the absorption spectra of various cell culture...

To characterize the profile of chromosomal imbalances in esophageal cancer (EC) with or without family history in Linzhou, Henan Province of China. Comparative genomic hybridization (CGH) was used to examine 13 cases with positive family history of EC and 32 cases with negative family history of EC. RESULTS DNA copy number gains on chromosome 10q w...

OBJECTIVE: To study the characteristics of the alteration of Survivin and E-cadherin protein expressions in concurrent carcinomas of the esophagus and gastric cardia from the same patient (CC). METHODS: Survivin and E-cadherin protein expressions were detected by immunohistochemical method (ABC) in 15 patients with concurrent esophageal squamous ce...

Objective: To investigate the relationship between human papillomavirus (HPV) infection and the development of esophageal squamous cell carcinoma (SCC) and gastric cardia adenocarcinoma(GCA) in the high risk area for esophageal cancer in Henan. Methods: HPV-DNA was detected by polymerase chain reaction (PCR) in 44 surgically resected specimens of S...

Endoglin (ENG) has been identified as a candidate tumor-suppressor gene in esophageal squamous cell carcinoma (ESCC). Earlier microcell-mediated chromosome transfer (MMCT) studies of chromosome 9 in ESCC narrowed down a tumor-suppressive critical region to 9q33-34. ENG maps to 9q34-qter and encodes a transformation growth factor beta (TGFbeta) supe...

Objective: To characterize the alterations and significance of p53 and proliferating cell nuclear antigen (PCNA) in esophageal epithelial dysplasia (EDYS) and gastric cardia dysplasia (GDYS) from the same patient. Methods: p53 and PCNA protein expressions were detected by the immunohistochemical method (ABC) in 38 patients with both EDYS and GDYS f...

Background: Nutrition deficiencies or poverty traditionally have been recognized to be related with increased risk for esophageal cancer (EC) in rural regions at junction of Henan, Hebei, and Shanxi provinces in northern China--the highest incidence area for esophageal squamous cell carcinoma (ESCC) and gastric cardia adenocarcinoma (GCA). Since t...

Cyclooxygenase-2 (COX-2) plays an important role in the carcinogenesis of esophageal squamous cell carcinoma (ESCC). However, it is not clear whether COX-2 is involved in the early or late stage of the development of ESCC. The aim of this study was to investigate the role of COX-2 in the carcinogenesis of ESCC by an immortalized esophageal epitheli...

To investigate the relationship between the human papillomavirus (HPV) and esophageal carcinomas. We detected HPV DNA in 31 fresh tissue of esophageal carcinomas from Linzhou City, Henan Province, by PCR with the general primer set of GP5+/6+ for HPV L1 gene and type-specific primer sets for HPV16 and 18 as well. 29 from 31 esophageal carcinoma sam...

To characterize cytogenetic alterations in esophageal squamous cell carcinoma (ESCC) and its metastasis. A total of 37 cases of primary ESCC and 15 pairs of primary ESCC tumors and their matched metastatic lymph nodes cases were enrolled from Linzhou, the high incidence area for ESCC in Henan, northern China. The comparative genomic hybridization (...

Previous studies showed that expression of the novel candidate tumor suppressor gene, DEC1 (Deleted in Esophageal Cancer 1), is reduced in esophageal carcinoma and suppresses cancer cell growth in vitro and tumor growth in vivo in nude mice. This study shows that DEC1 gene expression was downregulated in 100% of 16 esophageal squamous cell carcinom...

Objective: To investigate the effect and mechanism of the esophageal nerve transmitters on esophageal cancer cells differentiation and to provide clues for esophageal cancer prevention and treatment. Methods: EC109-line cells were divided into 6 groups: acetylcholine (Ach) group, Ach + Atropine group, norepinephrine (NE) group, NE + tolazoline grou...

AIM: To characterize the expression of P53 and proliferating cell nuclear antigen (PCNA) proteins in gastric cardiac adenocarcinoma (GCA) with and without paracancerous intestinal metaplasia (IM). METHODS: Immunohistochemical analysis (ABC method) was performed on tissues resected from 70 cases of GCA with and without paracancerous IM. RESULTS: The...

The lightest density of Mg has stimulated renewed interest in Mg based alloys for applications in the automotive, aerospace and communications industries. However, Mg in the pure form has relatively low strength, limited ductility and is susceptible to corrosion. Great efforts have been made to improve the mechanical properties of Mg alloys. Alloyi...

Magnesium borate whisker reinforced aluminum alloy 2024 (AA2024) composite was fabricated by squeeze casting method, after was CuO coating of the whisker. The interfacial reaction in the composite was investigated. To change the interfacial reaction degree, the solution treatment for different time was carried out. The results show that the interfa...

Extrusion experiments have been carried out on Bi2O3-coated Al18B4O33 whisker reinforced aluminum matrix composite. The effects of the extrusion temperature on the microstructures, the formability and the mechanical properties of ABOw reinforced aluminum (ABOw/Al) composites with and without Bi2O3 coating were investigated. The results show that th...

Our recent study on proteomics for esophageal cancer has indicated the importance of Annexin II as a promising protein to distinguish esophageal cancer patients from healthy subjects. This study was to detect the expression of Annexin II in esophageal squamous cell carcinoma (ESCC) and adjacent tissues, and to explore the role of Annexin II in ESCC...

Objective: To determine the significance of the tumor-associated antigen and autoantibody measurement in high-risk subject screening and early detection for esophageal cancer. Methods: The enzyme-linked immunoassay and tumor-associated antigen mini-array (consisting of eight full-length recombinant proteins, including C-myc, p53, cyclin B1, p62, p1...

In order to identify representative genetic alterations in esophageal squamous cell carcinomas (ESCC) and useful markers for future early detection, 34 ESCC samples with neighboring normal epithelia and 30 esophageal biopsy samples from Linzhou, P.R. China, were studied. Of the 38 microsatellite markers selected, half were linked with tumor suppres...

Objective: To establish the proteomic model for primary concurrent cancer of the esophagus and gastric cardia from the same patient (CC) and to identify the biomarkers associated with CC through proteomic analysis on serum from CC patients and healthy controls. Methods: SELDI-TOF-MS methods were applied to serum samples from CC patientss (n=19) and...

Objective: To characterize the p16 protein expression and promoter hypermethylation in gastric cardia carcinogenesis. Methods: Immunohisto-chemistry and methylation specific PCR were used to detect p16 protien expression and p16 gene promoter methylation in adjacent non-cancer tissue, dysplasia tissue (DYS) and gastric cardia adenocarcinoma (GCA) f...

A gene critical to esophageal cancer has been identified. Functional studies using microcell-mediated chromosome transfer of intact and truncated donor chromosomes 3 into an esophageal cancer cell line and nude mouse tumorigenicity assays were used to identify a 1.61 Mb tumor suppressive critical region (CR) mapping to chromosome 3p14.2. This CR is...