Lev Matveev

Russian Academy of Sciences | RAS · Institute of Applied Physics

The recent impressive progress in Compression Optical Coherence Elastography (C‐OCE) demonstrated diverse biomedical applications, comprising ophthalmology, oncology, etc. High resolution of C‐OCE enables spatially‐resolved characterization of elasticity of rather thin (thickness <1 mm) samples, which previously was impossible. Besides Young's modu...

In this paper, we present a new approach to contrast-agent-free angiographic visualization in optical coherence tomography (OCT). The proposed approach has much in common with imaging of local interframe strains in OCT-based elastography and utilizes the fact that the interframe motion of blood particles leads to discontinuity of strains within the...

In this paper, we offer a novel classification of progressive changes in the connective tissue of dermis in vulvar lichen sclerosus (VLS) relying on quantitative assessment of the second harmonic generation (SHG) signal received from formalin fixed and deparaffinized tissue sections. We formulate criteria for distinguishing four degrees of VLS deve...

Soft biological tissues, breast cancer tissues in particular, often manifest pronounced nonlinear elasticity, i.e., strong dependence of their Young’s modulus on the applied stress. We showed that compression optical coherence elastography (C-OCE) is a promising tool enabling the evaluation of nonlinear properties in addition to the conventionally...

We present in this study optical coherence tomography angiography (OCTA) and OCT attenuation imaging (OCTAI) for in vivo non-destructive visualization of intramural blood and lymphatic vessels of the intestine wall. Rabbit small intestine in the norm and after thoracolumbar sympathectomy served as the object of the intraoperative study. Compared to...

The aims of this study are (i) to compare ultrasound strain elastography (US-SE) and compression optical coherence elastography (C-OCE) in characterization of elastically linear phantoms, (ii) to evaluate factors that can cause discrepancy between the results of the two elastographic techniques in application to real tissues, and (iii) to compare t...

This paper presents a recently developed variant of phase-resolved Optical Coherence Elastography (OCE) enabling non-contact visualization of transient local strains of various origins in biological tissues and other materials. In this work, we demonstrate the possibilities of this new technique for studying dynamics of osmotically-induced strains...

We present a real-time realization of OCT-based elastographic mapping local strains and distribution of the Young’s modulus in biological tissues, which is in high demand for biomedical usage. The described variant exploits the principle of Compression Optical Coherence Elastography (C-OCE) and uses processing of phase-sensitive OCT signals. The st...

We present a computationally highly efficient full-wave spectral model of OCT-scan formation with the following features: allowance of arbitrary phase-amplitude profile of illuminating beams; absence of paraxial approximation; utilization of broadly used approximation of ballistic scattering by discrete scatterers without limitations on their densi...

Multimodal Optical Coherent Tomography grows popularity with researchers and clinicians over the past decade. One of the modalities is lymphangiography, which allows visualization of the lymphatic vessel networks within OCT imaging volume. In the present study, it is shown that lymphatic vessel visualization obtained from the depth-resolved attenua...

We present a computationally efficient full-wave spectral model of OCT-scan formation with the following capabilities/features: (i) the illuminating beam may have arbitrary phase-amplitude profile with allowance of a sharp diaphragm; (ii) paraxial approximation that limits the degree of focusing/divergence is not used; (iii) the broadly used approx...

A computationally efficient and fairly realistic model of OCT-scan formation in spectral-domain optical coherence tomography is described. The model is based on the approximation of discrete scatterers and ballistic character of scattering, these approximations being widely used in literature. An important feature of the model is its ability to eas...

There is a wide range of equivocal melanocytic lesions that can be clinically and dermoscopically indistinguishable from early melanoma. In the present work, we assessed the possibilities of combined using of multiphoton microscopy (MPM) and optical coherence angiography (OCA) for differential diagnosis of the equivocal melanocytic lesions. Clinica...

The possibility to assess molecular-biological and morphological features of particular breast cancer types can improve the precision of resection margin detection and enable accurate determining of the tumor aggressiveness, which is important for treatment selection. To enable reliable differentiation of breast-cancer subtypes and evaluation of re...

In this paper, we present a computationally highly efficient full-wave model of optical coherence tomography (OCT)-scan formation by focused beams in spectral-domain OCT. Similarly to some previous models, it is based on the summation of fields scattered by discrete sub-resolution scatterers, and enables one to account for the axial and lateral inh...

In this chapter we consider applications of multimodal OCT for characterizing tumorous tissues. Namely, the following extensions of OCT are discussed: (1) polarization-sensitive OCT providing complementary images in the initial and cross-polarization channels (CP OCT), (2) OCT-based label-free angiography (OCA), and (3) compressional variant of OCT...

In this study, for the first time, we apply optical coherence elastography to map and monitor slow strains caused by residual stresses in cartilaginous tissues subjected to thermomechanical laser-assisted reshaping in regimes used for the preparation of implants in otolaringological surgery. Such laser-fabricated implants made of the patient's rib...

Quantitative mapping of deformation and elasticity in optical coherence tomography has attracted much attention of researchers during the last two decades. However, despite intense effort it took ~15 years to demonstrate optical coherence elastography (OCE) as a practically useful technique. Similarly to medical ultrasound, where elastography was f...

We present a non-invasive (albeit contact) method based on Optical Coherence Elastography (OCE) enabling the in vivo segmentation of morphological tissue constituents, in particular, monitoring of morphological alterations during both tumor development and its response to therapies. The method uses compressional OCE to reconstruct tissue stiffness...

Pronounced elastic nonlinearity intrinsic to many biological tissues makes the Young's modulus (stiffness) of the tissue strongly dependent on the current strain and stress. The measured modulus may vary several times for rather moderate tissue straining within a few per cent, such strains being quite typical for compressional optical coherence ela...

A computationally highly efficient full-wave model of OCT-scan formation by focused beams in spectral-domain optical coherence tomography (OCT) is presented. Similarly to some previous models, it is based on summation of fields scattered by discrete sub-resolution scatterers and enables accounting for axial and lateral inhomogeneity of the illumina...

Emerging methods of anti-tumor therapies require new approaches to tumor response evaluation, especially enabling label-free diagnostics and in vivo utilization. Here, to assess the tumor early reaction and predict its long-term response, for the first time we apply in combination the recently developed OCT extensions - optical coherence angiograph...

We present a non-invasive method based on Optical Coherence Elastography (OCE) enabling the in vivo segmentation of morphological tissue constituents, in particular, monitoring of morphological alterations during both tumor development and its response to therapies. The method uses compressional OCE to reconstruct tissue stiffness map as the first...

Microvascular networks of human basal cell carcinomas (BCC) and surrounding skin were assessed with optical coherence angiography (OCA) in conjunction with photodynamic therapy (PDT). OCA images were collected and analyzed in 31 lesions pre-treatment, and immediately/24 hours/3–12 months post-treatment. Pre-treatment OCA enabled differentiation bet...

Moderate heating of collagenous tissues such as cartilage and cornea by infrared laser irradiation can produce biologically non‐destructive structural rearrangements and relaxation of internal stresses resulting in the tissue reshaping. The reshaping results and eventual changes in optical and biological properties of the tissue strongly depend on...

Analysis of semi-transparent low scattering biological structures in optical coherence tomography (OCT) has been actively pursued in the context of lymphatic imaging, with most approaches relying on the relative absence of signal as a means of detection. Here we present an alternate methodology based on spatial speckle statistics, utilizing the sim...

The clinical diagnosis of thin melanomas is an ongoing medical challenge. Non-invasive tools and technologies can help to distinguish equivocal lesions. The major focus of the study was to evaluate the diagnostic possibilities of label-free imaging modalities combination (multiphoton fluorescence microscopy, fluorescence lifetime imaging and optica...

A realization of real-time optical coherence elastography for mapping small-amplitude, slowly varying strains is discussed. The technique is based on the recently developed "vector" method for estimating interframe phase-variation gradients by considering complex-valued signals in optical coherence tomography (OCT) as vectors in the complex plane....