Lev Dykman

Lev Dykman
Saratov State University

About

174
Publications
38,769
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
7,476
Citations

Publications

Publications (174)
Article
The effects of gold nanoparticles (GNPs) on the redox status and freezing tolerance of wheat seedlings were studied. Here we show that treatment of winter wheat seedlings (Triticum aestivum L., var. Moskovskaya 39) with GNPs via their roots increased freezing tolerance by affecting the redox status of plants. Based on the assays with colloidal solu...
Article
Full-text available
The intensive development of nanotechnology led to the widespread application of various nanoparticles and nanomaterials. As a result, nanoparticles enter the environment and accumulate in ecosystems and living organisms. The consequences of possible impact of nanoparticles on living organisms are not obvious. Experimental data indicate that nanopa...
Article
Full-text available
Despite the decades of research, cancer causes millions of deaths every year. Therefore, the development of methods for the indication of tumor markers is very relevant. Heat shock proteins (HSPs) are important for oncogenesis and malignant progression and are a marker of cancer development. Here, HSP-specific antibodies were prepared by using a no...
Article
Full-text available
It was shown for the first time that the treatment of winter wheat (Triticum aestivum L.) seeds with gold nanoparticles (average diameter 15.3 nm; solution concentration 20 μg/mL) increases plant tolerance to low temperature. It was found that an increase in tolerance under the influence of nanoparticles is accompanied by a number of changes depend...
Article
Background The liver disease problem prompts investigators to search for new methods of liver treatment. Introduction Silymarin (Sil) protects the liver by reducing the concentration of free radicals and the extent of damage to the cell membranes. A particularly interesting method to increase the bioavailability of Sil is to use synthesized gold n...
Article
Silymarin (Sil) was conjugated to selenium nanoparticles (SeNPs) to increase Sil bioavailability. The conjugates were monodisperse; the average diameter of the native SeNPs was ~ 20-50 ± 1.5 nm, whereas that of the conjugates was 30-50 ± 0.5 nm. The use of SeNPs to increase the bioavailability of Syl was examined with the MH-22a, EPNT-5, HeLa, Hep-...
Article
This review deals with the influence of gold nanoparticles on physiological processes (responses) in higher plants. Gold nanoparticles can affect a lot of processes in the plant organism, including growth rate, parameters of water exchange, activity of the photosynthetic apparatus and the antioxidant system, and expression of some genes important f...
Conference Paper
The presence of non-pathogenic bacterial microflora in Arabidopsis thaliana suspension culture has been shown to coexist latently with plant cells for a long time. According to the results of 16S rRNA sequencing, it was shown that the detected bacteria belong to the species Rothia amarae. Using microbiological, microscopic and immunochemical method...
Conference Paper
Full-text available
We report, for the first time, that a nonpathogenic bacterial microflora has been found in a suspension culture of Arabidopsis thaliana cells. The 16S rRNA gene sequencing showed that the isolate belonged to Rothia amarae. Identification was confirmed by microbiological, microscopic, and immunochemical methods. The growth of the isolate on blood ag...
Article
Purpose: The aim of this study was to isolate Babesia canis soluble antigens and to investigate the effect of their conjugates with gold nanoparticles on the immunogenicity in laboratory animals. Methods: A procedure was developed for isolating and purifying B. canis antigens. The isolated culture antigen of B. canis 495 was coupled to gold nano...
Article
Information system was developed in the form of a web application that makes it possible to identify microscopic images of cytological samples, to establish an initial diagnosis and to provide recommendations for its further confirmation based on additional data. Approaches, assumptions and prerequisites adopted in the information system developmen...
Article
Full-text available
Introduction: Vaccination remains very effective in stimulating protective immune responses against infections. An important task in antibody and vaccine preparation is to choose an optimal carrier that will ensure a high immune response. Particularly promising in this regard are nanoscale particle carriers. An antigen that is adsorbed or encapsula...
Conference Paper
Full-text available
CLE peptides are important signaling molecules that regulate the balance of proliferation and differentiation of plant cells. In this work, we conjugated gold nanospheres (15 nm) with a chemically synthesized peptide (12 amino acid residues) that the amino acid sequence corresponds to the natural peptides CLE41/44. The "golden number" for CLE41/44...
Article
Full-text available
A number of bacterial glycans are specific markers for the detection and the serological identification of microorganisms and are also widely used as antigenic components of vaccines. The use of gold nanoparticles as carriers for glyco-epitopes is becoming an important alternative to the traditional conjugation with proteins and synthetic polymers....
Article
Full-text available
Speckle-microscopy is used for the detection of Chlamydia trachomatis in aqueous suspensions or on slides. Monoclonal antibody tagged with gold nanoparticles that form biospeckles have also been used with a small number of scatterers. By devising a prototype of laser scanning speckle-microscope, we now demonstrate that the signal of speckle microsc...
Article
Full-text available
Gold nanoparticle conjugates with Vibrio cholerae antigens were synthesized. The animals were immunized with the obtained conjugates. Rabbit polyclonal antibodies to the antigens were obtained, which showed high specific activity. On the model of white laboratory mice, the protective activity of conjugates of cholera antigens with nanoparticles dur...
Preprint
Full-text available
Some bacterial glycans are specific markers for detection and serological identification of microorganisms and are widely used as antigenic components of vaccines. The use of gold nanoparticles as carriers for glyco-epitopes is becoming an important alternative to the traditional conjugation with proteins and synthetic polymers. In this study, we a...
Article
Full-text available
This review presents a comparative analysis of the literature and the authors’ data on the ultrastructural reorganization of plant chloroplasts adapting to abiotic factors of different nature—low temperature (factor of a physical nature) and chloride salinity (factor of a chemical nature). All ultrastructural changes in chloroplasts are considered...
Article
Full-text available
Yersinia enterocolitica is the causative agent of yersiniosis, an acute infectious disease of the gastrointestinal tract. Y. enterocolitica is one of the most common causative agents of nosocomial infections. The problem of intestinal yersiniosis caused by Y. enterocolitica is very relevant for medicine and veterinary medicine and requires the use...
Article
Full-text available
Functionalized gold nanoparticles (GNPs) with controlled geometrical and optical properties have been the subject of intense research and biomedical applications. This review summarizes recent data and topical problems in nanomedicine that are related to the use of variously sized, shaped, and structured GNPs. We focus on three topical fields in cu...
Article
Full-text available
Published data on the chemical synthesis of colloidal gold are summarized and systematized. Attention is focused on the state-of-the-art concepts behind the mechanisms of citrate synthesis and its control parameters, methods for fabrication of ultrafine particles (1 – 5 nm) and the control over the particle spherical shape and size in the range fro...
Article
Here, for the first time, we report the presence of highly active extracellular carbonic anhydrase (CA) of α-class in cyanobacterial cells. The enzyme activity was confirmed both in vivo in intact cells and in vitro, using the recombinant protein. CA activity in intact cells of Cyanothece sp. ATCC 51142 reached ∼ 0.6 Wilbur–Anderson units (WAU) per...
Article
Full-text available
Recent years have seen extremely fast development of new viral nanovaccines and diagnostic agents using nanostructures prepared by biological and chemical synthesis. We used spherical gold nanoparticles (average diameter, 15 nm) as a platform for the antigen for swine transmissible gastroenteritis virus (TGEV). The literature data demonstrate that...
Article
Full-text available
The ability of the suspension culture of Arabidopsis thaliana cells and Dunaliella salina microalga to reduce gold ions with the formation of nanoparticles is shown. The average diameter of gold nanoparticles obtained using D. salina for synthesis appeared to be less than when using A. thaliana cells: 8 and 25 nm, respectively. Freshly prepared cul...
Article
Full-text available
Gold nanoparticles (AuNPs) are successfully used as an adjuvant in the design of effective vaccines and in the preparation of high-affinity antibodies to haptens and complete antigens. Here, we assessed the adjuvant properties of AuNPs conjugated to a synthetic M2e peptide of the influenza A virus capsid. The resulting conjugate, a commercial influ...
Article
Conjugates of gold nanoparticles with two isolated tularemia microbe antigens, a protective antigen complex and a glycosylated protein complex, are used to obtain anti-tularemia sera and to vaccinate animals. A conjugate of gold nanoparticles with the glycosylated protein complex during the subcutaneous immunization of mice is more effective than t...
Conference Paper
Gold nanoparticles are intensively studied in biomedicine. Assessment of their biocompatibility is highly important. Currently there is lack of evidence, concerning nanotoxicity of ultrasmall gold nanoparticles < 5 nm. Existing data are rather contradictory. The aim of that study was to evaluate the toxicity of 2 nm colloidal gold, using microalga...
Chapter
Full-text available
This review considers the past decade’s data regarding the effects of nanoparticles of noble metals (gold and silver) on higher plants, as well as regarding possible nanoparticle phytotoxicity. It discusses the various effects that gold and silver nanoparticles can have on the state, growth, and productivity of plants. Research in this field is top...
Book
This book discusses fabrication of functionalized gold nanoparticles (GNPs) and multifunctional nanocomposites, their optical properties, and applications in biological studies. This is the very first book of its kind to comprehensively discuss published data on in vitro and in vivo biodistribution, toxicity, and uptake of GNP by mammalian cells pr...
Article
Gold nanoparticles (GNPs) are advantageous as an adjuvant in the design of effective vaccines and in the preparation of high-affinity antibodies to haptens and complete antigens. Another method of activating immunocompetent cells with colloidal gold is to conjugate GNPs with CpG oligodeoxynucleotides (ODNs). We examined how the size and shape of GN...
Article
Full-text available
A method has been developed for monitoring the dynamics of the number and viability of Dunaliella salina microalgae taking into account the spectral contribution from aggregates of metal particles tested as a toxic agent in a microplate assay system. The method is based on the in vivo determination of the chlorophyll content from the intensity of a...
Article
The electro-acoustical sensor for the real-time identification of the bacteriophage FAl-SR65 is described. The polyclonal antibodies specific to the bacteriophage FAl-SR65 were for the first time obtained, and the possibility of their application to the bacteriophage detection by using the electro-acoustical method of analysis was also studied for...
Article
The past few years have seen substantial progress in veterinary oncology: new methods have been developed for the diagnosis and treatment of oncological diseases in animals, and the range of possible therapeutic interventions has been broadened. Of interest, in particular, are the prospects for the creation of veterinary pharmaceuticals with the us...
Article
Full-text available
The possibility of detecting bacteriophages using phage mini-antibodies by the electroacoustic analysis method using bacteriophages FA1-59b was shown. It was found that the frequency dependence of the real and imaginary parts of the electrical impedance of a resonator with a suspension of phages and the appropriate antibodies significantly differs...
Article
Full-text available
The work shows the ability of cultured Basidiomycetes of different taxonomic groups—Lentinus edodes, Pleurotus ostreatus, Ganoderma lucidum, and Grifola frondosa—to recover gold, silver, selenium, and silicon, to elemental state with nanoparticles formation. It examines the effect of these metal and metalloid compounds on the parameters of growth a...
Article
Full-text available
Gold and silver nanoparticles are used in a variety of biomedical practice as carriers of drugs, enhancers and/or converters of optical signal, immunomarkers, etc. The review examines a decade publications (2007-2016) pertaining to the various influence of nanoparticles of noble metals (gold and silver) on growth and productivity of higher plants....
Article
Full-text available
In the past decade, gold nanoparticles have attracted strong interest from the nanobiotechnological community owing to the significant progress made in robust and easy-to-make synthesis technologies, in surface functionalization, and in promising biomedical applications. These include bioimaging, gene diagnostics, analytical sensing, photothermal t...
Article
Full-text available
The addition of 20-nm gold and silver nanoparticles to a growth medium has a positive effect on the growth of biomass of a cell suspension culture of Arabidopsis thaliana (L.) Heynh. Growing the cells with silver and gold nanoparticles causes different changes in the pH of the medium: the culture medium is made markedly acidic by the addition of si...
Article
Full-text available
There was performed the study of immunogenic properties of the transmissible gastroenteritis virus antigen conjugated to gold nanoparticles. In comparative immunobiological studies there was found that immunization of guinea pigs driven by the colloidal gold conjugated to transmissible gastroenteritis virus antigen of swine, leads to activation of...
Article
Active application of gold nanoparticles for various diagnostic and therapeutic purposes started in recent decades due to the emergence of new data on their unique optical and physicochemical properties. In addition to colloidal gold con�jugates, growth in the number of publications devoted to the synthesis and application of multifunctional nanoco...
Article
Current laboratory and morphological differential diagnosis of prostate cancer is based on the use of sensitive tumor-specific markers. One of the most promising tumor markers is α-methylacyl-CoA racemase (AMACR), and studies indicate a positive correlation between AMACR expression, degree of tumor differentiation, and tumor progression. Most commo...
Article
It has been demonstrated that electroacoustic analysis with polyclonal antibodies can be used for bacteriophage detection. The frequency dependences of the real and imaginary parts of electrical impedance of a resonator with a viral suspension with antibodies were shown to be essentially different from the dependences of a resonator with control vi...
Article
The aim of this research was to design a method of immobilization of high-purity human butyrylcholinesterase on the surface of gold nanoparticles preserving the activity of the enzyme. In order to achieve this aim, the method of fractionation and purification of human butyrylcholinesterase from plasma was modified. The synthesis of 15-nm gold nanop...
Article
A diagnostic test system was developed to determine the toxicity of nanomaterials to the saltwater microalga Dunaliella salina through evaluation of cell death and changes in the culture growth rate at various toxicant concentrations, providing LC50 and other toxicological metrics. The viability of cells was shown to decrease with decreasing chloro...
Article
The possibility of the application of electro-acoustic analysis for the detection of bacteriophages was demonstrated for the first time based on the example of the interaction of the FA1-Sp59b bacteriophage with bacterial cells of the strain Azospirillum lipoferum Sp59b. Piezoelectric cross-field resonators with a 1-mL chamber for analyzed liquid w...
Article
Full-text available
The changes in the electroacoustic parameters of a cell suspension due to the interaction between cells and bacteriophages were studied both in pure culture and in the presence of extraneous microflora. It was shown that the specific changes in the electroacoustic parameters of a cell suspension under the influence of bacteriophage occur only in th...
Article
Full-text available
In the present study we investigate the effect of 5-nm gold nanoparticles on the proliferation and apoptosis of spermatogenesis in rats. It is found that the per os administration of gold nanoparticles to white rats for 8 weeks at a dose of 0.57 mg/kg/day did not change the expression of proliferation proteins (KI-67 and D1 cyclin) and did not alte...
Article
Full-text available
Foot-and-mouth disease is an acute, highly contagious infection of domestic and wild cloven-hoofed animals, which can be transmitted to humans. In many cases, the existing vaccines are not quite effective. The purpose of this study was to test the possibility of using gold nanoparticles as an antigen carrier and an adjuvant. The immunogenic propert...
Article
Full-text available
We are reporting a new diagnostic test system for evaluating nanomaterial toxicity on halophile green microalgae Dunaliella salina based on colorimetric measurements enabling us to estimate cell death and growth-rate inhibition in microalgae suspension for serial dilutions of several toxicants. The principle of the method is the photodegradation of...
Article
Vestnik RFFI (RFBR Journal). 2014. No. 4(84). P. 18-33 (in Russian)] Laboratory technologies of synthesis of composite nanoparticles containing plasmon resonant core (gold nanospheres, nanoshells, nanorods and gold-silver nanocages) and silica shell doped with photosensitizer with tuning of optical properties in visible and infrared regions were d...
Article
Full-text available
ABSTRACT The exposure of Azospirillum brasilense carbohydrate epitopes was investigated by electro-optical analysis of bacterial cell suspensions. To study changes in the electro-optical (EO) properties of the suspensions, we used antibodies (Abs) generated to the complete lipopolysaccharide (LPS) of A. brasilense type strain Sp7 and also antibodie...