Leslie Boudesocque-Delaye

• PhD, PharmD
• Professor (Full) at University of Tours

The growing demand for phycobiliproteins from microalgae generates a significant volume of by-products, such as extraction cakes. These cakes are enriched with products of interest for the cosmetics market, namely free fatty acids, particularly polyunsaturated (PUFA). In this work, two cakes, one of spirulina and one of Porphyridium cruentum, were...

The growing demand for phycobiliproteins from microalgae generates a significant volume of by-products, such as extraction cakes. These cakes are enriched with products of interest for the cosmetic market, namely free fatty acids, particularly polyunsaturated (PUFA). In this work, two cakes, one of spirulina and one of Porphyridium cruentum, were v...

Natural deep eutectic solvents (NaDES), a category of sustainable solvents made from biorefinery ingredients, are an eco-friendly alternative to organic solvents in solubilization of active pharmaceutical or cosmetic ingredients. Biocompatible NaDES can be introduced in final products but are prone to modify their properties thanks to their hydroge...

Cyathostomins are the most prevalent parasitic nematodes of grazing horses. They are responsible for colic and diarrhea in their hosts. After several decades of exposure to synthetic anthelmintics, they have evolved to become resistant to most compounds. In addition, the drug-associated environmental side-effects question their use in the field. Al...

The escalating consumer demand for sustainable cosmetic ingredients poses distinct challenges, particularly concerning their stability within the final formulation. Although natural resources offer a pool of antioxidant molecules with diverse structures and polarities, achieving stabilization combined with a comprehensive antioxidant profile often...

To meet the criteria of modern extraction, it is essential to use solvents that simultaneously reach the standards of green chemistry and allow the extraction of a wide spectrum of bio-compounds. Natural Deep Eutectic Solvents (NaDES), as a green solution, meet this difficult task and represent a satisfactory alternative to organic solvents. Microa...

The use of volatile solvents for organic synthesis is nowadays questioned due to their negative impact on the environnement. To develop sustainable and environmentally friendly methodologies, we propose to combine two green chemistry concepts: the use of bioinspired solvents: natural deep eutectic solvents (NaDES), and mechanochemistry. Using the S...

The current control of gastrointestinal (GI) parasitic nematodes mainly relies on the widespread use of anthelmintics, which has inevitably led to resistance. Therefore, there is an urgent need to find new sources of antiparasitic compounds. Macroalgae represent a rich source of active molecules and are widely described as having medicinal properti...

The growing demand for molecules of interest from microalgal biomass, such as phycobiliproteins, has led to an accumulation of unused by-products. For example, phycocyanin, obtained by the extraction of Spirulina, generated cakes rich in non-polar molecules of interest, such as free fatty acids (FFAs). These FFAs were generally considered as marker...

Vibrational spectroscopic techniques, i.e., attenuated total reflectance infrared (ATR-IR), near infrared spectroscopy (NIRS) and Raman spectroscopy (RS), coupled with Partial Least Squares Regression (PLSR), were evaluated as cost-effective label-free and reagent-free tools to monitor water content in Levulinic Acid/L-Proline (LALP) (2:1, mol/mol)...

Phycocyanin, a natural blue phycobiliprotein extracted from cyanobacteria, recently focused the interest of food industry as promising pigment and antioxidant. Eco-extraction of phycocyanin via ultrasound using five glycerol-based NaDES was described here. Response surface methodology (RSM) and artificial neural network (ANN) were adapted for the g...

Helminth infections of livestock result in economic losses around the globe. The majority of the people in West Africa treats such infections with medicinal plants related to the local traditional medicine. In a previous ethnomedicinal survey carried out in the north of Togo, traditional healers frequently cited Cassia sieberiana DC to treat helmin...

Attenuated total reflectance-infrared spectroscopy (ATR-IR) coupled with partial least squares regression (PLSR) was evaluated as a rapid, label free and cost-effective tool to quantify water content in extracts obtained from spirulina wet biomass using a glucose glycerol natural deep eutectic solvent (NADES). NADESs are green, renewable and biodeg...

With the growing interest in more environmentally friendly solvents and processes, the introduction of Natural Deep Eutectic Solvents (NaDES) as low cost, non-toxic and biodegradable solvents represent a new opportunity for green and sustainable chemistry. Thanks to their remarkable advantages, NaDES are now arousing growing interest in many fields...

Raman spectroscopy is a label-free, non-destructive, non-invasive analytical tool that provides insight into the molecular composition of samples with minimum or no sample preparation. The increased availability of commercial portable Raman devices presents a potentially easy and convenient analytical solution for day-to-day analysis in laboratorie...

Post-doctoral fellowship 18 months Research team: EA 7502 Synthèse et Isolement de Molécules Bioactives (SIMBA) https://simba.univ-tours.fr/ Referee: Pr Leslie Boudesocque-Delaye leslie.boudesocque@univ-tours.fr https://www.linkedin.com/in/leslie-boudesocque-delaye-a441701b Project: ANR JCJC 2019 natural Deep Eutectic solvents foR Microalgae bIo...

Microalgae are a sustainable source of bioactive compounds like carotenoids, free fatty acids and especially polyunsaturated fatty acids. The extraction of such nonpolar metabolites usually involved nonpolar or toxic solvent like methanol or hexane. We report the first screening of Natural Deep Eutectic Solvents for the extraction of pigments and f...

Natural deep eutectic solvents (NADES) are ionic solutions, of great interest for extraction from biomass, biocatalysis, and nanoparticle synthesis. They are easily synthesised and eco-friendly, have low volatility and high dissolution power, and are biodegradable. However, water content in NADES is a critical parameter, affecting their optimal use...

Background/objectives: Hibiscussabdariffa L. (H.sabdariffa (HS)) extract has a vascular relaxant effect on isolated rat thoracic aorta, but data on small resistance arteries, which play an important role on the development of hypertension, are still missing. The purposes of this study were (1) to assess the effect on isolated mesenteric arteries (...

Biofilm-related infections are a matter of concern especially because of the poor susceptibility of microorganisms to conventional antimicrobial agents. Innovative approaches are needed. The antibiofilm activity of extracts of cyanobacteria Arthrospira platensis, rich in free fatty acids, as well as of extract-loaded copper alginate-based nanocarri...

Natural Deep Eutectic solvents (NADES) are green, ionic solutions prepared by mixing vegetal cellular constituents. They are renewable and biodegradable solvents composed of hydrogen bonded donor and acceptor compounds. NADES have a wide range of applications, mainly as solvents for extraction of plants, biocatalysis, and nanoparticle synthesis. Wa...

The growing range of drug resistant parasitic nematode populations threatens the sustainability of ruminant farming worldwide. In this context, nutraceuticals, animal feed that provides necessary dietary requirements while ensuring parasite control, could contribute to increase farming sustainability in developed and low resource settings. In this...

The growing expansion range of drug resistant parasitic nematode populations threatens the sustainability of ruminant farming worldwide. In this context, nutraceuticals, i.e. feed that would both fulfil dietary requirements while ensuring parasite control, would contribute to increase farming sustainability in developed and low resource settings. I...

Cyanobacteria and especially Spirulina platensis are recognized as abundant resources of lipids and poly-unsaturated fatty acids (PUFA). The main valorization of these lipids has been the biofuel production but another approach was developed here: the investigation of S. platensis fatty acids as anti-biofilm agent for skin diseases. In fact, chroni...

Herein, we present the first Suzuki–Miyaura cross-coupling in a sustainable natural deep eutectic solvent (NaDES) applied to biologically relevant imidazo-fused scaffolds imidazo[1,2-a]pyridine and imidazo[1,2-b]pyridazine. The choline chloride/glycerol (1:2, mol/mol) NaDES allowed the functionalisation of diverse positions on the heterocycles with...

A fast quality control protocol based on reference BL-DMAC assay and High-performance thin-layer chromatography (HPTLC)-Densitometry was optimized for routine quality control of cranberry (Vaccinium macrocarpon) products. The sample preparation appeared as a key step to ensure a relevant comparison of products quality and biological data. Sequentia...

Dirucotide is a synthetic drug candidate for the treatment of multiple sclerosis. This 17-aminoacid peptide was successfully purified by ion exchange centrifugal partition chromatography. The optimized conditions involved the biphasic methyl tert-butyl ether/acetonitrile/n-butanol/water (2:1:2:5, v/v) solvent system in the descending mode, the di(2...

Post-doctroal position to work on extraction of microalgae using NADES for cosmetic purpose

Ethnopharmacoligical relevance: Leaves of Crateva adansonii DC (Capparidaceae), a small bush found in Togo, are widely used in traditional medicine to cure infectious abscesses. Traditional healers of Lomé harvest only budding leaves early in the morning, in specific area in order to prepare their drugs. Aim of the study: The main goal was to va...

Le lupin, protéagineux déjà utilisé en nutrition animale, contient des alcaloïdes qui pourraient avoir des vertus anthelminthiques. Notre étude s’attache à valider et quantifier ces propriétés par des tests in vitro exposant Haemonchus contortus, strongle parasite de ruminants, à différents extraits de deux variétés, riche (amère) ou pauvre (douce)...

Aristolochic acids are biologically active and highly toxic secondary metabolites that recently became an important pharmacological tool for in vivo models of acute kidney disorders. Only aristolochic acid I and a mixture of aristolochic acids I and II are currently commercially available. A method based on the strong-ion exchange displacement mode...

Two heterodimers comprising anthraquinone and methylbenzoisocoumarin moieties (1 and 2) were isolated, together with emodin and physcion from the tubers of Pyrenacantha kaurabassana. The structures of 1 and 2 were established by NMR spectroscopy, including the analysis of a 2D INADEQUATE spectrum. On the basis of the data obtained, the structures t...

ABSTRACT: Cranberry is recognized for its many benefits on human health; however, its high acidity may be a limiting factor for its consumption. This study aimed to investigate the deacidification of cranberry juice using a two simultaneous step electrodialysis with bipolar membranes (EDBM) process. In step 1 (deacidification), during the 6 h treat...

trans-Resveratrol (1a) is a phytoalexin produced by plants in response to infections by pathogens. Its potential activity against clinically relevant opportunistic fungal pathogens has previously been poorly investigated. Evaluated herein are the candidacidal activities of oligomers (2a, 3-5) of 1a purified from Vitis vinifera grape canes and sever...

Candida guilliermondii (teleomorph Meyerozyma guilliermondii) is an ascomycetous species belonging to the fungal CTG clade. This yeast remains actively studied as a result of its moderate clinical importance and most of all for its potential uses in biotechnology. The aim of the present study was to establish a convenient transformation system for...

A new type 1 ternary biphasic system composed of cyclopentyl methyl ether, dimethylformamide and water was developed, characterized and successfully used for the purification of a lipophilic, protected peptide by pH-zone refining centrifugal partition chromatography. The protected peptide is an 8-mer, key intermediate in bivalirudin (Angiomax®) syn...

In the context of dental caries prevention by natural foodstuff sources, antifungal and anti-biofilm activities of dry commercial extracts of cranberry fruit (Vaccinium macrocarpon Aiton) and two other red fruits (Vaccinium myrtillus L. and Malpighia punicifolia L.) were assessed on Candida albicans and Candida glabrata yeasts. When added to the cu...

The roots of Ptaeroxylon obliquum Radlk. (Rutaceae) afforded a new meroterpenoid, ptaerobliquol, together with chromones, ptaeroxylinol acetate and peucenin, and coumarins, scopoletin and prenyletin. Ptaerobliquol was isolated using centrifugal partition chromatography followed by silica gel column and identified as 7a,8,9,9a,9b,10a-heptahydro-4H-1...

Synthetic hydrophobic non-ionizable peptides are not soluble in most common solvents and are thus difficult to purify by preparative reversed-phase HPLC, normally used for industrial production. The challenge exists to develop alternative purification chromatographic processes using suitable solvents and providing good yields, high purity and suffi...

This article reports a promising use of the mixed ion-exchange centrifugal partition chromatography (MIXCPC) technique in the field of downstream processes. A complex alfalfa white protein concentrate hydrolysate (AWPC hydrolysate) showing anti-hypertensive properties was successfully fractionated by MIXCPC to yield a l-valyl-l-tryptophan (VW) enri...

Cranberry (Vaccinium macrocarpon) focused the interest of scientist for years now, due to assessed biological activities such as anti infectious, anti inflammatory or anti cancer activity. Anti infectious activity is linked to Proanthocyanidins A (PAC A), major PACs in cranberry. To characterize PACs content of cranberry extracts, official method i...

This article presents the scope and optimization strategies employed in ion-exchange centrifugal partition chromatography (IXCPC). Both the weak and the strong modes were used to separate the constituents of a model mixture of dipeptides. Thus, the combined use of the quaternary biphasic solvent system, methyl-tert-butylether/acetonitrile/n-butanol...

Glucosinolates (GSLs) are a class of phytochemicals found in all cruciferous plants (Brassicaceae) family as well as in the whole order of Brassicales (syn. Capparales). GSLs and their hydrolysis products (e.g. isothiocyanates) are known to play a defensive role by protecting the plant against exterior aggressions and may be potent antitumor, antic...

NMR of weakly polar analytes in an apolar ultraviscous solvent has recently been proposed for mixture analysis as a pertinent alternative to the DOSY experiment. The present article reports the first use of glycerol and glycerol carbonate as polar solvents for the NMR analysis of a model mixture of dipeptides. This work demonstrates the high potent...

The purification of p-hydroxybenzylglucosinolate (sinalbin) on a multigram scale from a crude aqueous extract of white mustard seeds (Sinapis alba var. concerta) was successfully achieved by scaling up a strong ion-exchange centrifugal partition chromatography (SIXCPC) laboratory procedure. Thus, the one-step sinalbin purification was performed wit...

Rhamnolipids produced by the bacteria Pseudomonas aeruginosa are known as very efficient biosurfactant molecules. They are used for a wide range of industrial applications, especially in food, cosmetics and pharmaceutical formulations as well as in bioremediation of pollutants. In this paper, the role of rhamnolipids as novel molecules triggering d...

Original Paper Pilot-scale ion-exchange centrifugal partition chromatography: Purification of sinalbin from white mustard seeds The purification of p-hydroxybenzylglucosinolate (sinalbin) on a multigram scale from a crude aqueous extract of white mustard seeds (Sinapis alba var. concerta) was successfully achieved by scaling up a strong ion-exchang...

Nowadays, centrifugal partition chromatography (CPC) separations can be routinely achieved at the laboratory scale. The solvent system selection has been made easy, as generic sets of solvent systems are described in publications and books. This approach, however, generally reduces the scope of optimization strategies for two important parameters:...