Lesław B Lahuta

Lesław B Lahuta
University of Warmia and Mazury in Olsztyn · Department of Plant Physiology Genetics and Biotechnology

Professor

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86
Publications
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1,261
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Publications

Publications (86)
Article
Full-text available
Myo-inositol plays a key role in the vasculature and may be beneficial for preventing harmful environmental effects. In this study aortic rings were isolated from middle-aged (12-month-old) male Wistar rats and preincubated with myo-inositol (0.01–100 mg/L) for 2 h. A stable thromboxane A2 analog was added (0.1 nM, 2 h) to analyze vascular dysfunct...
Article
Full-text available
Nano-priming is a relatively new seed treatment technique using metal and metal oxide nanoparticles (NPs), and such application of NPs may support the plants' immunity. Recently we have shown that the that biologically synthesized silver nanoparticles (bio-AgNPs) used as short-term foliar treatment protect pea seedlings against D. pinodes and F. av...
Article
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Cadmium (Cd) is a key stress factor that affects plant development. To examine the influence of Cd stress, we analysed the tissue localisation of polysaccharides (Periodic Acid Schiff reaction), qualitative and quantitative changes in soluble carbohydrates (High-Resolution Gas Chromatography), and the expression of the galactinol synthase (PsGolS)...
Preprint
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A potential protective effect of myo–inositol against pro–oxidant damage to human plasma and rat thoracic arteries has been demonstrated, suggesting that this nutraceutical agent plays a key role in the vasculature and may be beneficial for preventing harmful environmental effects. Aortic rings were isolated from aged (12–month–old) male Wistar rat...
Article
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Ascochyta blight and Fusarium root rot are the most serious fungal diseases of pea, caused by D. pinodes and F. avenaceum, respectively. Due to the lack of fully resistant cultivars, we proposed the use of biologically synthesized silver nanoparticles (bio-AgNPs) as a novel protecting agent. In this study, we evaluated the antifungal properties and...
Article
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Seed-to-seedling transition plays a crucial role in plant vegetation. However, changes in the metabolome of crop seedlings during seed germination and early seedling development are mostly unknown and require a deeper explanation. The present study attempted to compare qualitative and quantitative changes in polar metabolites during the seed germin...
Article
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The growing interest in the use of zinc oxide nanoparticles (ZnO NPs) in agriculture creates a risk of soil contamination with ZnO NPs, which can lead to phytotoxic effects on germinating seeds and seedlings. In the present study, the susceptibility of germinating seeds/seedlings of pea and wheat to ZnO NPs of various sizes (≤50 and ≤100 nm) applie...
Article
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Zmiany zawartości D-chiro-inozytolu i jego α-D-galaktozydowych pochodnych podczas wegetacji i desykacji gryki zwyczajnej (Fagopyrum esculentum Moench) Summary. Common buckwheat (Fagopyrum esculentum Moench) is the only crop that contains D-chiro-inositol (DCI) in significant contents in vegetative tissues and its α-D-galactosyl derivatives in seeds...
Article
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Plant species are sensitive to stresses, especially at the seedling stage, and they respond to these conditions by making metabolic changes to counteract the negative effects of this. The objectives of this study were to determine carbohydrate profile in particular organs (roots, hypocotyl, and cotyledons) of common buckwheat seedlings and to verif...
Article
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The response of wheat (Triticum aestivum L.) plants to the soil drought at the metabolome level is still not fully explained. In addition, research focuses mainly on single periods of drought, and there is still a lack of data on the response of plants to short-term cyclical periods of drought. The key to this research was to find out whether wheat...
Article
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Citation: Lahuta, L.B.; Szablińska-Piernik, J.; Stałanowska, K.; Horbowicz, M.; Górecki, R.J.; Railean, V.; Pomastowski, P.; Buszewski, B. Exogenously Applied Cyclitols and Biosynthesized Silver Nanoparticles Affect the Soluble Carbohydrate Profiles of Wheat (Triticum aestivum L.) Seedling. Plants 2023, 12, 1627. https://doi. Abstract: Cyclitols, s...
Article
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Here, we compared the polymorphism among 13 Avena species revealed by the iPBS markers and soluble carbohydrate profiles in seeds. The application of seven iPBS markers generated 83 bands, out of which 20.5% were polymorphic. No species-specific bands were scored. Shannon's information index (I) and expected heterozygosity (H e) revealed low geneti...
Article
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Citation: Saniewski, M.; Szablińska-Piernik, J.; Marasek-Ciołakowska, A.; Mitrus, J.; Góraj-Koniarska, J.; Lahuta, L.B.; Wiczkowski, W.; Miyamoto, K.; Ueda, J.; Horbowicz, M. Accumulation of Anthocyanins in Detached Leaves of Abstract: Accumulation of anthocyanins in detached leaves and in excised stems of Kalanchoë blossfeldiana kept under natural...
Article
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The phytotoxicity of silver nanoparticles (Ag NPs) to plant seeds germination and seedlings development depends on nanoparticles properties and concentration, as well as plant species and stress tolerance degrees. In the present study, the effect of citrate-stabilized spherical Ag NPs (20 mg/L) in sizes of 10, 20, 40, 60, and 100 nm, on wheat grain...
Article
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Myo-inositol is the most popular inositol in living organisms, where it is present as a sugar alcohol, in a free form, and can also be described as a lipid. The aim of this study was to check the concentration change of a myo-inositol solution from the time of oral administration and over a 48 h period in Wistar-type rats. All rats received 2 g/kg...
Article
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The field pea (Pisum sativum L.) is studied as an important grain legume used in both human and animal feed. DNA markers can contribute to the rapid breeding of novel pea cultivars. This study aimed to identify such molecular markers as the number of days to the beginning of flowering, plant lodging, and stem geometry. Phenotypic measurements were...
Article
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The use of silver nanoparticles (Ag NPs) on plants is accompanied by the occurrence of Ag+ ions, so the research of the effects of both on plants should be related. Therefore, in our study, the effects of Ag NPs suspension (containing Ag0 at 20 mg/L) and AgNO3 solutions (with the concentration of Ag+ ions at 20 and 50 mg/L) on the seed germination...
Article
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Changes in the metabolome of germinating seeds and seedlings caused by metal nano-particles are poorly understood. In the present study, the effects of bio-synthesized silver nanopar-ticles ((Bio)Ag NPs) on grains germination, early seedlings development, and metabolic profiles of roots, coleoptile, and endosperm of wheat were analyzed. Grains germ...
Article
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Oxidative stress induces functional changes in arteries. Therefore, the effect of myo-inositol, a possible anti-inflammatory/antioxidant agent was studied on human plasma and rat thoracic arteries. Aortic rings from male Wistar rats (3 months of age) were incubated with myo-inositol (1, 10 and 100 μM, 120 min) and analyzed using the gas chromatogra...
Article
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The metabolic re-arrangements of peas (Pisum sativum L.) under soil drought and re-watering are still not fully explained. The search for metabolic markers of the stress response is important in breeding programs, to allow for the selection drought-resistant cultivars. During the present study, changes in the polar metabolite content in pea plant s...
Article
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The conditions for determining the antioxidant properties of cyclitols (d-pinitol, l-quebrachitol, myo-, l-chiro-, and d-chiro-inositol), selected flavanones (hesperetin, naringenin, eriodictyol, and liquiritigenin) and glutathione by spectrophotometric methods—CUPRAC and with DPPH radical, and by a chromatographic method DPPH-UHPLC-UV, have been i...
Chapter
Cyclitols are high polarity polyols, belonging to secondary metabolites, which are easily isolable from plant material. These compounds are biosynthetically derived from glucose, and occur in all living cells. They form a group of biologically active compounds and participate in many cellular processes such as: membrane biogenesis, signal transduct...
Article
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Ten willow cultivars grown in experimental plots were evaluated for performance, attractiveness to foragers, and the content and composition of soluble carbohydrates. The survival of willow cuttings in a thicket and in browse plots differed subject to cultivar, soil quality, and soil moisture content. The number of stump sprouts varied considerably...
Article
Four semi-leafless pea (Pisum sativum L.) cultivars at the vegetative stage of growth were exposed to progressive soil drought, which lasted for 18 days until the plants began to wilt, after which a 7-day period of the recovery from stress followed, when plant watering was resumed. The soil drought negatively affected plant growth, slowing down the...
Article
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Cyclitols play a particularly important role in cell functioning because they are involved in ion channel physiology, phosphate storage, signal transduction, cell wall formation, membrane biogenesis, osmoregulation and they have antioxidant activity. They are involved in the cell membranes as a phosphatidyl myo-inositol, an inositol triphosphate pr...
Article
The composition and content of low-molecular weight soluble carbohydrates in seeds of 66 accessions of common vetch (Vicia sativa L. ssp. sativa) stored in the Polish and German gene banks, originating from Europe, Asia, Middle East and North Africa, were analyzed using a gas chromatography method. Genotypes showed differences in the composition of...
Article
The aim of the present study was to develop an optimization procedure for supercritical fluid extraction parameters, in order to obtain the highest possible yield of sugars and cyclitols from plant material. Response surface methodology based on Box‐Behnken design was applied to evaluate the effect of: temperature (40, 60, 80 °C), pressure (100, 20...
Article
Methylated inositol, d‐pinitol (3‐O‐methyl‐d‐chiro‐inositol), is a common constituent in legumes. It is synthesized from myo‐inositol in two reactions: the first reaction, catalyzed by myo‐inositol‐O‐methyltransferase (IMT), consists of a transfer of a methyl group from S‐adenosylmethionine to myo‐inositol with formation of d‐ononitol, while the se...
Article
Kernels of winter triticale (Triticosecale Wittm. cv. Dinaro) were analyzed. In the autumn of 2015, the effect of methyl jasmonate (MJ) on the germination of triticale kernels and the development of triticale seedlings was analyzed in a laboratory before kernels were sown in experimental plots. Kernels harvested from plots in August 2016 were analy...
Article
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Introduction: Myo-inositol and its derivatives cyclitols play an important role in the processes of cell regulation, signal transduction, osmoregulation, and ion channel physiology, and are a component of the cell membrane. Free cyclitols present in food or released during the degradation of galactosyl cyclitols by bacteria (in digestive tract) sho...
Article
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This article presents changes in concentrations of d-pinitol (and other cyclitols as well as low molecular weight carbohydrates) in vegetative and reproductive organs of fenugreek (Trigonella foenumgraecum L.) during an entire plant growing period. d-Pinitol was the major cyclitol in all tested organs, representing 43–94% of total cyclitols and 2–7...
Article
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Low molecular weight carbohydrates of seeds of 10 species of Vicia, namely: V. angustifolia, V. articulata, V. cordata, V. ervilia, V. johannis, V. macrocarpa, V. monantha, V. narbonensis, V. pannonica and V. sativa were analyzed by the high resolution gas chromatography method. Seeds of the investigated species contain common (glucose, fructose, m...
Article
Introduction: Cyclitols, the hydroxylated cycloalkanes biosynthetically derived from glucose, occur in all living cells and express a broad spectrum of biological activity and participate in many cellular processes including membrane biogenesis, signal transduction, ion channel physiology, osmoregulation, and antioxidation. They have several health...
Article
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The objective of this study was to analyze the mechanism of some physiological processes accompanying acquisition of sunflower (Helianthus annuus L.) chilling resistance due to seeds hydropriming in the presence of salicylic acid, jasmonic acid, 24-epibrassinolide followed exposition of seeds to short-term heat shock treatment. The seeds were hydro...
Article
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The content of raffinose family oligosaccharides (RFOs) in pea seeds constrains their usage in feeding humans and animals. In our research, the content of soluble carbohydrates—particularly α-d-galactosides of sucrose (RFOs)—was analyzed. The materials were as follows: 248 accessions from the Polish Pisum Genebank including representatives of taxa...
Article
The effect of Methyl Jasmonate (MJ, jasmonic acid methyl ether) at 10⁻⁶-10⁻³M concentrations on triticale kernels germination, seedling growth, changes in soluble carbohydrates content and composition, and activity of α-amylase was studied. MJ inhibited the germination of triticale kernels, possibly due to decreasing activity of α-amylase, leading...
Article
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Solidago gigantea Ait. (goldenrod) belongs to the most expansive environmental weeds, and it is the most dangerous plant-invader of American origin in Europe. The species easily propagates vegetatively, but it also produces large amounts of wind-disseminated achenes that contribute to the colonization of new areas. A sound knowledge of the germinat...
Article
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Background At initial steps of rehydration from cryptobiosis of anhydrobiotic organisms or at rehydration of dry tissues the liquid ¹H NMR signal increased anomaly. The surplus in liquid signal may appear if some solid constituents dissolved, or if they were decomposed by enzymatic action. Methods Hydration kinetics, sorption isotherm, ¹H NMR spec...
Article
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The objective of the present study was to recognize the molecular background of the accumulation of raffinose family oligosaccharides (RFOs) in pea (Pisum sativum L.) seedlings under osmotic stress conditions. The exposure of 5-day-old pea seedlings to osmotic stress for 48 h created by immersing roots in PEG8000 solution (−1.5 MPa) induced synthes...
Article
Galactinol is the galactosyl donor for the biosynthesis of both the raffinose family oligosaccharides (RFOs) and galactosyl cyclitols (Gal-C). Its synthesis by galactinol synthase (GolS, EC 2.4.1.123) is the first committed step of the soluble α-d-galactosides biosynthetic pathway in orthodox seeds. The deposition of galactosides in seeds is sugges...
Article
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The accumulation of soluble carbohydrates in maturing diaspores of flowering plants comprising Arctic populations of Cerastium alpinum, indigenous Antarctic species Colobanthus quitensis and Deschampsia antarctica, and cosmopolitan Poa annua from the Antarctic was investigated. For comparative purposes, the diaspores of two species of flowering pla...
Poster
Full-text available
The study showed significant variation in content of dietary fibre and its five components among different varieties of pea and added new knowledge about their nutritive values and possible uses.
Article
The exposition of 7-day-old pea seedlings to dehydration induced sudden changes in the concentration of monosaccharides and sucrose in epicotyl and roots tissues. During 24 h of dehydration, the concentration of glucose and, to a lesser extent, fructose in seedling tissues decreased. The accumulation of sucrose was observed in roots after 4 h and i...
Article
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Four species of flowering plants comprising Arctic populations of Cerastium alpinum and Poa arctica var. vivipara and indigenous Antarctic species Colobanthus quitensis and Deschampsia antarctica were investigated. Plants derived from natural origins were grown in an experimental greenhouse in Poland (53°47′N and 20°30′E latitude). Plants for exper...
Article
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Anti-nutritional compounds are among the obstacles to the use of pea seeds as a protein source in both feed and food. These compounds are poorly digested by both monogastric animals and humans. There are three main oligosaccharides in pea: raffinose, stachyose and verbascose (raffinose family oligosaccharides - RFOs). The concentration of oligosacc...
Article
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The present paper discusses the effects of α-aminooxyacetic acid (AOA) on contents of polyamines, anthocyanins, photosynthetic pigments and phenylalanine ammonia-lyase activity in seedlings of common buckwheat (Fagopyrum esculentum Moench). AOA clearly decreased light-induced formation of anthocyanins and inhibited PAL activity in buckwheat hypocot...
Article
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The role of the abscisic acid (ABA) in biosynthesis of raffinose family oligosaccharides (RFOs) and galactosyl cyclitols (Gal-C) in tiny vetch (Vicia hirsuta [L.] S.F. Gray) seeds was investigated. The ABA was applied through incubation of seed at various stage of its development. The level of RFOs and Gal-C was determined in seed maturing on plant...
Article
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Effect of Lupin Cyclitols on Pea Aphid Probing Behaviour The cyclitols: D-pinitol, D- chiro -inositol are naturally present in the tissues of Lupinus angustifolius . The effect of these cyclitols on the behaviour of the pea associated clone of Acyrthosiphon pisum during various stages of probing was studied. The main stage of probing studied was th...
Article
Feeding stem–leaf–pod explants with d-chiro-inositol and d-pinitol was used as a method to modify α-d-galactosides in developing pea (Pisum sativum) seeds. Four genotypes differing in the composition of raffinose, stachyose and verbascose (raffinose family oligosaccharides or RFOs) in seeds – high RFOs (cv. Tiny), low RFOs (SZD175) and low verbasco...
Article
We compared the soluble carbohydrate composition of seeds of ten wild and cultivated species of the genus Vicia. In some Vicia species (V. angustifolia, V. grandiflora, V. sativa, V. sepium) they contained only raffinose family oligosaccharides (RFOs) and in others also D-pinitol and its α-D-galactosides. In terms of galactosyl pinitol composition...
Article
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This study compares the soluble sugar content of zygotic and somatic pea embryos. It was noted that mature somatic embryos differed from zygotic embryos with respect to carbohydrate composition. Mature zygotic pea embryos contained glucose, myo-inositol, sucrose, maltose, galactinol, galactosyl-cyclitols, raffinose, stachyose and verbascose. The pr...
Article
Developing garden pea embryos are able to take up exogenously applied cyclitols: myo-inositol, which naturally occurs in pea, and two cyclitols absent in pea plants: d-chiro-inositol and d-pinitol. The competition in the uptake of cyclitols by pea embryo, insensitivity to glucose and sucrose, and susceptibility to inhibitor(s) of H(+)-symporters (e...
Article
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In the present study, the feeding of stem-flag leaf-ear explants of wheat, triticale and barley with D-chiro-inositol and D-pinitol was used for modification of the composition of soluble carbohydrates in grains without genetic transformation of plants. Maturing grains indicated ability to uptake exogenously applied cyclitols, not occurring natural...
Article
The mechanism preferentially regulating accumulation of raffinose family oligosaccharides (RFOs) or galactosyl cyclitols in legume seeds still remains unknown. The broad range of raffinose family oligosaccharides and galactosyl pinitols in the composition of seeds of Vicia genus gives researchers an exceptional opportunity for investigations on rel...
Article
During germination of winter vetch (Vicia villosa Roth.) seeds, the degradation of raffinose family oligosaccharides and galactosyl pinitols occurred faster in axis than in cotyledons. After 7days of germination, all α-d-galactosides disappeared and the soluble carbohydrates in seedling tissues consisted of d-pinitol, sucrose, fructose, glucose and...
Article
Mature seeds of yellow lupine contained sucrose, raffinose family oligosaccharides (RFOs), and galactosyl cyclitols as major soluble carbohydrates. The study showed that RFOs dominated in lupine seeds (16% DW). The disappearance of both types of alpha-d-galactosides in germinating lupine seeds was strongly inhibited by the presence of jasmonic acid...
Article
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We examined the effects of cooling applied for 4 to 20weeks on donor cultures of four dwarfing apple rootstocks (P16, P22, P59 and M26). Our aim includes increasing their competence for in vitro adventitious shoot regeneration from the leaves. Donor cultures were maintained on a shoot multiplication medium at 4°C in the dark for 4months, followed b...
Article
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Beside RFOs, which are commonly present in legume seeds, seeds of some species contain galactosyl pinitols (GPs). These carbohydrates, like RFOs, have been hypothesized to constitute an important energy and carbon skeletal source during germination. To test this hypothesis we have applied a specific α-galactosidase inhibitor (1-deoxygalactonojirimy...
Article
We attempted to establish biochemical markers for the selection of more winter-hardy genotypes, on the basis of the contents of cryoprotectants (proline and soluble sugars) in in vitro shoot cultures. The proline and sugar contents were measured in acclimated and non-acclimated shoot explants of six raspberry and two blackberry cultivars. In experi...
Article
The pea aphid, Acyrthosiphon pisum Harris (Homoptera: Aphididae), fed, developed, and reproduced on yellow lupine, Lupinus luteus L. (Fabaceae: Genisteae). No clear preferences for any variety within L. luteus were found. Acyrthosiphon pisum showed negative values of relative growth rate and no aphid completed development on any variety of narrow-l...
Article
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Yellow lupin seeds cv. Juno were stored under laboratory conditions for 2 month, 4, 6 and 8 years. Eighteen soluble carbohydrates were identified in embryonic axes and cotyledons of different age seeds. The concentration of soluble carbohydrates in analysed seeds ranged from 25 to 34% of dry mass. Axes contained more carbohydrates than cotyledons....
Article
The aim of the present study was to determine the effect of accelerated ageing on the composition and content of the soluble carbohydrates in pea seeds of six genotypes differing in the composition of raffinose family oligosaccharides. A gradual decrease in the concentration of higher homologues of raffinose was observed along with seed ageing. At...
Article
Full-text available
Changes in the accumulation of two types of α-D-galactosides: raffinose family oligosaccharides and galactosyl pinitols were compared with changes in the activities of galactosyltransferases during winter vetch (Vicia villosa Roth.) seed development and maturation. Occurrence of galactinol and raffinose in young seeds and changes in activities of g...
Article
Full-text available
In the present study we have investigated the effect of exogenous cyclitols on the accumulation of their galactosides and raffinose family oligosaccharides (RFOs), as well as on some enzymes important for their biosynthesis in seeds of tiny vetch (Vicia hirsuta [L.] S.F. Gray). Immature seeds during 6-day incubation with D-chiro-inositol (naturally...
Article
In the present study we have investigated the effect of exogenous cyclitols on accumulation of their galactosides and raffinose family oligosaccharides (RFOs) in maturing smooth tare (Vicia tetrasperma [L.] Schreb) seeds. Feeding d-pinitol to pods of smooth tare increased the amount of free d-pinitol and its galactosides: galactopinitol A, galactop...
Article
Composition and levels of soluble α-galactosides: raffinose family oligosaccharides (RFOs) and galactosyl cyclitols (Gal-C) in developing seeds were measured by high resolution gas chromatography (HRGC) method. The studies were performed on maturing seeds of several wild and cultivated Vicia species: Vicia angustifolia L. (common vetch), Vicia crac...
Article
The molecular structure of galactosyl-D-(-)-bornesitol, a novel compound isolated from sweet pea seeds, was determined to be alpha-D-galactopyranosyl-(1-->3)-1-O-methyl-1D-myo-inositol by 1D and 2D NMR spectroscopy and is assigned the trivial name lathyritol.
Article
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Raffinose family oligosaccharides (RFOs) are synthesized by a set of galactosyltransferases, which sequentially add galactose units from galactinol to sucrose. The accumulation of RFOs was studied in maturing seeds of two pea (Pisum sativum) lines with contrasting RFO composition. Seeds of the line SD1 accumulated stachyose as the predominant RFO,...
Article
Full-text available
Raffinose family oligosaccharides (RFOs) are synthesized by a set of galactosyltransferases, which sequentially add galactose units from galactinol to sucrose. The accumulation of RFOs was studied in maturing seeds of two pea (Pisum sativum) lines with contrasting RFO composition. Seeds of the line SD1 accumulated stachyose as the predominant RFO,...
Article
Carbohydrate analysis was made of yellow lupin seeds (cv. Juno) and triticale kernels (cv. Dagro), produced by plants exposed to drought stress for 21 days after the initial flowering of the first node of lupin and initial earing of triticale. The seeds of all experimental variants were harvest at full maturity, dried and stored in linen bags at 18...
Chapter
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This book is the result of a combined effort from scientists covering many disciplines interacting within a European Union-funded Copernicus project entitled 'Carbohydrate Biotechnology Network for Grain Legumes' (CABINET). The CABINET project linked 30 participants from 14 countries across Europe, including states of the former Soviet Union. The 8...