Leopoldo Santos-Argumedo

Leopoldo Santos-Argumedo
Center for Research and Advanced Studies of the National Polytechnic Institute | Cinvestav · Departamento de Biomedicina Molecular

PhD

About

182
Publications
19,401
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3,422
Citations
Additional affiliations
January 1999 - present
Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional
January 1993 - December 1999
Instituto Politécnico Nacional (IPN)
Position
  • Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional
October 1991 - July 1993
DNAX Research Institute, Palo Alto Cal.
Education
August 1980 - September 1989
Escuela Nacional de Ciencias Biológicas del IPN.
Field of study
  • Immunology

Publications

Publications (182)
Article
Background Common Variable Immunodeficiency (CVID) is a heterogeneous disorder characterized by defective B cell differentiation and antibody production. Interleukin (IL)-21 activates STAT3, a potent regulator of B cell differentiation into plasma cells. We have studied the phosphorylation of STAT3 in CVID patients and its contribution to B cells s...
Article
Full-text available
NK cells are contained in the ILC1 group; they are recognized for their antiviral and antitumor cytotoxic capacity; NK cells also participate in other immune response processes through cytokines secretion. However, the mechanisms that regulate these functions are poorly understood since NK cells are not as abundant as other lymphocytes, which has m...
Article
Full-text available
Citation: Pérez-Lara, J.C.; Espinosa, E.; Santos-Argumedo, L.; Romero-Ramírez, H.; López-Herrera, G.; García-García, F.; Sandoval-Montes, C.; Ortiz-Navarrete, V.; Flores-Muñoz, M.; Rodríguez-Alba, J.C. CD38 Correlates with an Immunosuppressive Treg Phenotype in Lupus-Prone Mice. Abstract: CD38 is a transmembrane glycoprotein expressed by T-cells. I...
Article
Full-text available
Helicobacter pylori is a gram-negative bacterium found on the luminal surface of the gastric mucosa in at least 50% of the world's human population. The protective effect of breastfeeding against H. pylori infection has been extensively reported; however, the mechanisms behind this protection remain poorly understood. Human IgA from colostrum has r...
Article
Full-text available
Microbiota acquired during labor and through the first days of life contributes to the newborn’s immune maturation and development. Mother provides probiotics and prebiotics factors through colostrum and maternal milk to shape the first neonatal microbiota. Previous works have reported that immunoglobulin A (IgA) secreted in colostrum is coating a...
Data
It was built using STRING (Search Tool for the Retrieval of Interacting Genes/Proteins; http://www. string-db.org). The PPI was generated with confidence score ≥ 0.4. The network contains 39 nodes and 78 edges. Likewise, grouping of Rab35-S22N -interacting proteins into biological processes was carried out using Gene Ontology (GO) annotation (http:...
Article
Full-text available
Cell spreading and phagocytosis are notably regulated by small GTPases and GAP proteins. TBC1D10C is a dual inhibitory protein with GAP activity. In immune cells, TBC1D10C is one of the elements regulating lymphocyte activation. However, its specific role in macrophages remains unknown. Here, we show that TBC1D10C engages in functions dependent on...
Article
Full-text available
The coronavirus disease 2019 (COVID-19) pandemic has reached an unprecedented level. There is a strong demand for diagnostic and serological supplies worldwide, making it necessary for countries to establish their own technologies to produce high-quality biomolecules. The two main viral antigens used for the diagnostics for severe acute respiratory...
Preprint
Full-text available
Background: Microbiota colonization during labor and through the first meals contributes to immune maturation and development of the newborn. Mother provides probiotics and prebiotics factors through colostrum and maternal milk to shape the first neonatal microbiota. Previous works have reported that immunoglobulin A (IgA) secreted in colostrum is...
Article
Full-text available
Cell migration is a dynamic process that involves adhesion molecules and the deformation of the moving cell that depends on cytoskeletal remodeling and actin-modulating proteins such as myosins. In this work, we analyzed the role of the class I Myosin-1 g (Myo1g) in migratory processes of LPS + IL-4 activated B lymphocytes in vivo and in vitro. In...
Article
During immune responses, B cells home to lymph nodes (LNs), where they encounter antigens. Homing starts with capture and L-selectin-dependent rolling on the activated endothelium of high endothelial venules (HEV). After recognition of chemokines presented on HEV, activation of B cell integrins occurs mediating firm arrest. Subsequently, B cells cr...
Article
Full-text available
Dengue virus infection (DENV-2) is transmitted by infected mosquitoes via the skin, where many dermal and epidermal cells are potentially susceptible to infection. Most of the cells in an area of infection will establish an antiviral microenvironment to control viral replication. Although cumulative studies report permissive DENV-2 infection in den...
Article
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Antecedentes: Las deficiencias de anticuerpos abarcan un amplio espectro de patologías y constituyen aproximadamente 50 % de las inmunodeficiencias primarias; con la citometría es posible evaluar el estado inmunológico de forma rápida, efectiva y a bajo costo. Objetivo: Evaluar mediante citometría de flujo, las células de pacientes con tres tipos...
Article
Background: Antibody deficiencies encompass a wide spectrum of pathologies and constitute approximately 50 % of primary immunodeficiencies; with cytometry, it is possible to evaluate the immune status rapidly, effectively and at low cost. Objective: To assess, by means of flow cytometry, the cells of patients with three types of primary humoral...
Article
Lipopolysaccharide (LPS)‐responsive beige‐like anchor (LRBA) protein was initially described as a monogenetic cause for Common Variable Immune Deficiency, a syndrome characterized by low levels of B‐cells, defects in memory B‐cell differentiation, and hypogammaglobulinemia. LRBA was identified as an LPS‐up‐regulated gene in B‐cells, macrophages, an...
Article
Full-text available
Background: Several studies have evaluated the effect of infectious diseases and vaccine protocols during pregnancy on maternal milk immunoglobulin (Ig) levels, to understand the protection conferred by lactation on newborns. Colostrum is the primary source of maternal IgA for the newborn. IgA participates in protection mechanisms in the neonate's...
Article
Full-text available
The inclusion of lymphocytes in high endothelium venules and their migration to the lymph nodes are critical steps in immune response. Cell migration is regulated by actin cytoskeleton and myosins. Myo1e is a long-tailed class I myosin and is highly expressed in B cells that have not been studied in the context of cell migration. By using intravita...
Article
B lymphocytes are a leukocyte subset capable of developing several functions apart from differentiating into antibody‐secreting cells. These processes are triggered by external activation signals that induce changes in the plasma membrane properties, regulated by the formation of different lipid‐bilayer subdomains that are associated with the under...
Article
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Neutrophil extravasation is a migratory event in response to inflammation that depends on cytoskeletal dynamics regulated by myosins. Myosin-1e (Myo1e) is a long-tailed class-I myosin that has not yet been studied in the context of neutrophil–endothelial interactions and neutrophil extravasation. Intravital microscopy of TNFα-inflamed cremaster mus...
Chapter
Most patients with ectodermal dysplasia-immunodeficiency (ED-ID) or incontinentia pigmenti (IP) harbor hemizygous/hypomorphic mutations in the IKBKG gene which encodes the protein NEMO
Chapter
Full-text available
The autosomal dominant form of hyper IgE syndrome (HIES) has both immunologic and non-immunologic features
Article
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Zika virus (ZIKV), an emerging mosquito-borne flavivirus, has quickly spread in many regions around the world where dengue virus (DENV) is endemic. This represents a major health concern, given the high homology between these two viruses, which can result in cross-reactivity. The aim of this study was to determine the cross-reacting antibody respon...
Article
Full-text available
Hyper-IgE syndrome (HIES) is a rare primary immunodeficiency characterized by elevated levels of immunoglobulin E (IgE), eczematous dermatitis, cold abscesses, and recurrent infections of the lung and skin caused by Staphylococcus aureus . The dominant form is characterized by nonimmunologic features including skeletal, connective tissue, and pulmo...
Preprint
Full-text available
The recruitment of leukocyte to high endothelium venules and their migration to the lymph nodes are critical steps to initiate an immune response. Cell migration is regulated by the actin cytoskeleton where myosins have a very import role. Myo1e is a long tail class I myosin highly expressed in B cells that not have been studied in the context of c...
Article
Full-text available
Background: Colostrum is the primary source of maternal immunoglobulin A (IgA) for the newborn. IgA participates in protection and regulation mechanisms of the immune response at the neonate's mucosa. Several studies have evaluated infectious diseases and vaccine protocols effects during pregnancy on maternal milk IgA levels, with the aim to under...
Article
Background: Common variable immunodeficiency (CVID) is a heterogeneous group of primary antibody deficiencies defined by marked reductions in serum IgG, IgA and/or IgM levels and recurrent bacterial infections. Some patients are associated with defects in T cells and regulatory T cells (Tregs), resulting in recurrent viral infections and early-ons...
Article
Full-text available
Connections established between cytoskeleton and plasma membrane are essential in cellular processes such as cell migration, vesicular trafficking, and cytokinesis. Class I myosins are motor proteins linking the actin‐cytoskeleton with membrane phospholipids. Previous studies have implicated these molecules in cell functions including endocytosis,...
Article
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Intestinal macrophages are highly mobile cells with extraordinary plasticity and actively contribute to cytokine-mediated epithelial cell damage. The mechanisms triggering macrophage polarization into a proinflammatory phenotype are unknown. Here, we report that during inflammation macrophages enhance its intercellular adhesion properties in order...
Article
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A novel cell population denominated IFN‐γ‐producing killer dendritic cells (IKDCs), has been recently described. These cells are lymphocytes lacking B‐ or T‐ receptors, but they can be identified by the presence of B220⁺ CD38⁺ CD49b⁺ and low CD11c, among other cell surface markers. The main characteristics of IKDCs are the production of IFN‐γ and t...
Article
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La microbiota humana es el conjunto de microorganismos que residen en nuestro cuerpo. Su composición filogenética está relacionada con el riesgo de padecer enfermedades inflamatorias y cuadros alérgicos. Los humanos interaccionamos con una gran cantidad y variedad de estos microorganismos a través de la piel y las mucosas. Un mecanismo de protecció...
Article
The skin is the first anatomical region that dengue virus (DENV) encounters during the natural infection. Although the role of some skin resident cells like dendritic cells and fibroblasts has been demonstrated to be crucial to elucidate the role of resident cells and molecules participating during the early events of the innate immune response, th...
Article
Full-text available
CD38 is a transmembrane protein expressed in B lymphocytes, that is able to induce responses as proliferation, differentiation or apoptosis. Several reports propose that CD38 deficiency accelerates autoimmune processes in murine models of autoimmune diabetes, lymphoproliferation, and rheumatoid arthritis. Other reports have shown elevated CD38 expr...
Article
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Naegleria fowleri is a protozoan that invades the central nervous system and causes primary amoebic meningoencephalitis. It has been reported that N. fowleri induces an important inflammatory response during the infection. In the present study, we evaluated the roles of Toll-like receptors in the recognition of N. fowleri trophozoites by human muco...
Article
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Cell migration and adhesion are critical for immune system function and involve many proteins, which must be continuously transported and recycled in the cell. Recycling of adhesion molecules requires the participation of several proteins, including actin, tubulin, and GTPases, and of membrane components such as sphingolipids and cholesterol. Howev...
Article
Background: X-linked agammaglobulinemia (XLA) is characterized by the absence of immunoglobulin and B cells. Patients suffer from recurrent bacterial infections from early childhood, and require lifelong immunoglobulin replacement therapy. Mutations in BTK (Bruton’s Tyrosine Kinase) are associated with this phenotype. Some patients that present XLA...
Article
Full-text available
Background Better knowledge of the innate immune system of insects will improve our understanding of mosquitoes as potential vectors of diverse pathogens. The ubiquitously expressed 14-3-3 protein family is evolutionarily conserved from yeast to mammals, and at least two isoforms of 14-3-3, the ε and ζ, have been identified in insects. These protei...
Article
Tetraspanins are a family of transmembrane proteins that form membrane microdomains. They play important roles in migration, adhesion, and other cellular processes. TspanC8, a subfamily of tetraspanins, were found to associate and promote ADAM10 trafficking and cell surface localization. One of its members, Tspan33, is expressed in activated B cell...
Article
Background: Peripheral blood B cells include lymphocytes at various stages of differentiation, each with a specific function in the immune response. All these stages show variations in percentage and absolute number throughout human life. The numbers and proportions of B subpopulation are influenced by factors such as gender, age, ethnicity, and l...
Article
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Hyper IgE syndrome (HIES) is characterized by recurrent skin abscesses, eczema, pneumonia, and high levels of serum IgE. Non-immunologic manifestations of HIES include a characteristic face, pathologic dentition, scoliosis, bone alterations, hyperextensible joints, and vascular abnormalities. Somatic mosaicism is defined by the presence of two or m...
Article
Haemophagocytic lymphohistiocytosis (HLH) may be triggered by infections with or without association with classical primary immunodeficiencies. Viruses are the most common triggers, however, many others microorganisms have been linked such as mycobacterial infection. We followed up on two patients previously reported with IL-12Rβ1 deficiency who de...
Article
B-lymphocytes are migrating cells that specialize in antigen presentation, antibody secretion, and endocytosis; these processes implicate the modulation of plasma membrane elasticity. Cell stiffness is a force generated by the interaction between the actin-cytoskeleton and the plasma membrane, which requires the participation of several proteins. T...
Article
X-linked agammaglobulinemia (XLA) is caused by BTK mutations, patients typically show < 2% of peripheral B cells and reduced levels of all immunoglobulins; they suffer from recurrent infections of bacterial origin; however, viral infections, autoimmune-like diseases, and an increased risk of developing gastric cancer are also reported. In this work...
Article
Full-text available
For many years, it has been considered that the function of B cells is only to serve as precursors of antibody-producing plasma cells; however, this simplistic view has been challenged in the past thirty years. The first big surprise came during the seventies, when it was shown that B lymphocytes are not a homogeneous population, but is made up of...
Conference Paper
Common Variable Immunodeficiency (CVID) is a primary immunodeficiency characterized by B cell dysfunction and decreased serum immunoglobulin. CVID patients are classified by the absence or presence of memory B cells. In addition, T cell defects have been demonstrated in only a proportion of CVID patients. The aim of this study was to evaluate the f...
Article
Full-text available
Hyper-IgE syndrome is an immunodeficiency disorder that is characterized by distinctive immunologic and non-immunologic manifestations. Although mutations in STAT3 have been associated with HIES, the exact nature of the relationship is unknown. Here, we characterized the functional activity of STAT3 and its mutations in eleven Mexican patients with...
Article
Full-text available
We analyzed data from 71 patients with chronic granulomatous disease (CGD) with a confirmed genetic diagnosis, registered in the online Latin American Society of Primary Immunodeficiencies (LASID) database. Latin American CGD patients presented with recurrent and severe infections caused by several organisms. The mean age at disease onset was 23.9...
Article
The X-linked hyper-IgM syndrome (XHIGM) is the most common form of HIGM. Patients are clinically diagnosed on the basis of recurrent sinopulmonary infections, accompanied with low levels of IgG and IgA, normal to elevated levels of IgM, and the presence of peripheral B cells. Here, we have reported a novel deletion of four nucleotides in CD40LG exo...
Chapter
Bruton’s tyrosine kinase (BTK) is a well-studied molecule that is known to play a critical role in pre-B and B cell receptor signaling. It is crucial for early B cell development in the bone marrow and for B cell activation and differentiation in peripheral lymphoid organs. In addition to its role in B cell development and function, BTK is also exp...
Article
Full-text available
Two patients with a severe leukocyte adhesion deficiency type 1 (LAD-1) phenotype were analyzed by flow cytometry and functional assays to demonstrate the improper adhesive and phagocytic responses of their leukocytes. A single homozygous defect that involves a missense mutation (c.817G>A) that encodes for a G273R substitution in CD18 was identifie...