Lenka Záveská Drábková

Callose is a plant-specific polysaccharide (β-1,3-glucan) playing an important role in angiosperms in many developmental processes and responses to biotic and abiotic stresses. Callose is synthesised at the plasma membrane of plant cells by callose synthase (CalS) and, among others, represents the main polysaccharide in the callose wall surrounding...

Juncus trifidus and J. monanthos, two species traditionally included in the section Steirochloa, the Juncaceae, are shown to be substantially different morphologically from the other members of this section. Their relationships with the other groups recognized in the family, using DNA data sets of selected regions of plastome, chondriome and the nr...

The Juncaceae is a species-rich family (443 species, seven genera) with widespread distribution in temperate and arctic regions in both hemispheres. A phylogenetic analysis of the Juncaceae was conducted to assess relationships within and between the genera Juncus, Luzula and five small mainly South American genera (Distichia, Marsippospermum, Oxyc...

Bryophytes represent a very diverse group of non-vascular plants such as mosses, liverworts and hornworts and the oldest extant lineage of land plants. Determination of endogenous phytohormone profiles in bryophytes can provide substantial information about early land plant evolution. In this study, we screened thirty bryophyte species including si...

Nuclear sequences of ITS1-5.8S-ITS2 region of rDNA may be an important source of phylogenetically informative data provided that nrDNA is cloned and the character of sequence variation of clones is properly analyzed. nrDNA of selected Taraxacum sections was studied to show sequence variation differences among diploid sexual, tetraploid sexual and p...

Phylogenetic relationships within the genus Juncus L. remained poorly understood for most groups, and the taxonomic division to two subgenera and ten sections was rather pragmatic. The taxonomic treatment based on the morphology of this group is complicated due to numerous repeated reductions. Continuing the molecular phylogenetic, taxonomic, and n...

Based on comprehensive and long-time phylogenetic investigations, newly established Juncaceae genera have recently been published. These new genera comprise Verojuncus Záveská Drábková & Proćków, Juncinella Záveská Drábková & Proćków, Alpinojuncus Záveská Drábková & Proćków, Australojuncus Záveská Drábková & Proćków, Boreojuncus Záveská Drábková &...

Telomere repeat binding proteins (TRBs) belong to a family of proteins possessing a Myb-like domain which binds to telomeric repeats. Three members of this family (TRB1, TRB2, TRB3) from Arabidopsis thaliana have already been described as associated with terminal telomeric repeats (telomeres) or short interstitial telomeric repeats in gene promoter...

RAD51 is involved in finding and invading homologous DNA sequences for accurate homologous recombination (HR). Its paralogs have evolved to regulate and promote RAD51 functions. The efficient gene targeting and high HR rates are unique in plants only in the moss Physcomitrium patens (P. patens). In addition to two functionally equivalent RAD51 gene...

Telomere repeat binding proteins (TRBs) belong to a family of proteins possessing a Myb-like domain which binds to telomeric repeats. Three members of this family (TRB1, TRB2, TRB3) from Arabidopsis thaliana have already been described as associated with terminal telomeric repeats (telomeres) or short interstitial telomeric repeats in gene promoter...

Telomere repeat binding proteins (TRBs) belong to a family of proteins possessing a Myb-like domain which binds to telomeric repeats. Three members of this family (TRB1, TRB2, TRB3) from Arabidopsis thaliana have already been described as associated with terminal telomeric repeats (telomeres) or short interstitial telomeric repeats in gene promoter...

Juncaceae is a cosmopolitan family belonging to the cyperid clade of Poales together with Cyperaceae and Thurniaceae. These families have global economic and ethnobotanical significance and are often keystone species in wetlands around the world, with a widespread cosmopolitan distribution in temperate and arctic regions in both hemispheres. Curren...

Plant microgametogenesis involves stages leading to the progressive development of unicellular microspores into mature pollen. Despite the active and continuing interest in the study of male reproductive development, little is still known about the hormonomics at each ontogenetic stage. In this work, we characterized the profiles and dynamics of ph...

In the complex process of homeostasis of phytohormones cytokinins (CKs), O -glucosylation catalyzed by specific O- glucosyltransferases represents one of important mechanisms of their reversible inactivation. The CK O- glucosyltransferases belong to a highly divergent and polyphyletic multigene superfamily of glycosyltransferases, of which subfamil...

Being rooted in place, plants are faced with the challenge of responding to unfavourable local conditions. One such condition, heat stress, contributes massively to crop losses globally. Heatwaves are predicted to increase, and it is of vital importance to generate crops that are tolerant to not only heat stress but also to several other abiotic st...

ALBA DNA/RNA-binding proteins form an ancient family, which in eukaryotes diversified into two Rpp25-like and Rpp20-like subfamilies. In most studied model organisms, their function remains unclear, but they are usually associated with RNA metabolism, mRNA translatability and stress response. In plants, the enriched number of ALBA family members re...

With the expansion of molecular techniques, the historical collections have become widely used. The last boom started with using next- and second-generation sequencing in which massive parallel sequencing replaced targeted sequencing and third-generation technology involves single molecule technology. Studying plant DNA using these modern molecular...

Cytokinins (CKs) are a class of phytohormones affecting many aspects of plant growth and development. In the complex process of CK homeostasis in plants, N-glucosylation represents one of the essential metabolic pathways. Its products, CK N7- and N9-glucosides, have been largely overlooked in the past as irreversible and inactive CK products lackin...

Tobacco (Nicotiana tabacum) pollen is a well-suited model for studying many fundamental biological processes owing to its well-defined and distinct development stages. It is also one of the major agents involved in the transmission of infectious viroids, which is the primary mechanism of viroid pathogenicity in plants. However, some viroids are non...

Some viroids-single-stranded, non-coding, circular RNA parasites of plants-are not transmissible through pollen to seeds and to next generation. We analyzed the cause for the elimination of apple fruit crinkle viroid (AFCVd) and citrus bark cracking viroid (CBCVd) from male gametophyte cells of Nicotiana tabacum by RNA deep sequencing and molecular...

FULL TEXT AVAILABLE HERE: https://www.tandfonline.com/eprint/JMQ4BTRVMV6D6PV4AKNX/full?target=10.1080/07357907.2019.1663208 Ovarian cancer is the deadliest gynecologic cancer. The large-scale microRNA (miRNA) expression profiling and individual miRNA validation was performed to find potential novel biomarkers for ovarian cancer. The most consisten...

Ovarian cancer as the most fatal gynecological malignancy is often manifested by excessive fluid accumulation known as ascites or effusion. Ascites-derived microRNAs (miRNAs) may be closely associated with ovarian cancer progression. However, our knowledge of their roles, altered expression, and clinical outcomes remained limited. In this study, la...

Telomerase maturation and recruitment to telomeres is regulated by several telomerase‐ and telomere‐associated proteins. Among a number of proteins, human Pontin and Reptin play critical roles in telomerase biogenesis. Here we characterize plant orthologues of Pontin and Reptin, RuvBL1 and RuvBL2a, respectively, and show association of Arabidopsis...

Phylogenetic and barcoding studies usually use fresh plant tissues as sources of DNA and have successfully amplified DNA for various loci. The use of dried samples, however, is often necessary due to the frequent inaccessibility of fresh rare plants or their parts for genetic analyses or barcoding. The difficulty in obtaining amplifiable DNA is a m...

Phylogenetic tree and classification of 1211 plant sequences of 12 Arabidopsis callose synthase proteins. The evolutionary history was inferred by using maximum likelihood; the tree with the highest log likelihood (-979085.0765) is shown. The analysis included a total of 4150 positions in the final data set. Main branches with circles are described...

Phylogenetic tree of the pollen-specific CalS5 (GLS2) protein rooted with Ginkgo biloba. The timetree shown was generated using RelTime. The estimated log likelihood value of the topology shown is -57276.0114. A discrete gamma distribution was used to model the differences in evolutionary rate amongst sites (five categories (+G, parameter = 0.5505)...

Phylogenetic tree of pollen-expressed CalS11 (GLS1) proteins rooted with Spirodella polyrhiza. The timetree shown was generated using RelTime. The estimated log likelihood value of the topology shown is -54506.2366. A discrete gamma distribution was used to model the differences in evolutionary rate amongst sites (five categories (+G, parameter = 0...

Phylogenetic tree of pollen-expressed CalS 12 (GLS5) proteins rooted with Physcomitrella patens. The timetree shown was generated using RelTime. The estimated log likelihood value of the topology shown is -79253.3574. A discrete gamma distribution was used to model the differences in evolutionary rate amongst sites (five categories (+G, parameter =...

Callose synthase amino acid alignment data set. (NEX)

Phylogenetic tree of pollen-expressed CalS9 (GLS10) proteins rooted with Physcomitrella patens. The timetree shown was generated using RelTime. The estimated log likelihood value of the topology shown is -91929.9006. A discrete gamma distribution was used to model the differences in evolutionary rate amongst sites (five categories (+G, parameter =...

Domain sequence distribution in different callose synthases from the Pfam Motif Library. Highlighted domains are found in different plants. (XLSX)

Phylogenetic tree of pollen-expressed CalS10 (GLS8) proteins rooted with Amborella trichopoda. The timetree shown was generated using RelTime. The estimated log likelihood value of the topology shown is -54519.4579. A discrete gamma distribution was used to model the differences in evolutionary rate amongst sites five categories (+G, parameter = 0....

Consensus-sequence blocks of plant callose synthases. Sequence logos indicates main 50 conserved sequence patterns. (DOCX)

The genus Spiraea includes > 100 species of deciduous shrubs that are widespread in the temperate and subtropical zone of the Northern Hemisphere. We analysed AFLP markers with four primer pairs and scored 203 polymorphic fragments in 59 Spiraea clones, representing 32 taxa (23 species) from all four currently recognized sections (Spiraea, Calospir...

Pollen, an extremely reduced bi-cellular or tri-cellular male reproductive structure of flowering plants, serves as a model for numerous studies covering wide range of developmental and physiological processes. The pollen development and subsequent progamic phase represent two fragile and vital phases of plant ontogenesis and pollen was among the f...

Background and Aims The metabolism of cytokinins (CKs) and auxins in vascular plants is relatively well understood, but data concerning their metabolic pathways in non-vascular plants are still rather rare. With the aim of filling this gap, 20 representatives of taxonomically major lineages of cyanobacteria and algae from Cyanophyceae, Xanthophycea...

Background. Phylogenetic and barcoding studies usually employ fresh parts of plants as the source of DNA. Successful DNA amplification has been achieved in such investigations for different regions. However, there is need for the utilization of dried samples, due to frequent inaccessibility of fresh precious plants or their parts for genetic analys...

Background. Phylogenetic and barcoding studies usually employ fresh parts of plants as the source of DNA. Successful DNA amplification has been achieved in such investigations for different regions. However, there is need for the utilization of dried samples, due to frequent inaccessibility of fresh precious plants or their parts for genetic analys...

Among gynaecological cancers, epithelial ovarian cancers are the most deadly cancers while endometrial cancers are the most common diseases. Efforts to establish relevant novel diagnostic, screening and prognostic markers are aimed to help reduce the high level of mortality, chemoresistance and recurrence, particularly in ovarian cancer. MicroRNAs,...

The genus Taraxacum is characterized by prevailing complex multiple hybridity, frequent polyploidy and widespread agamospermous reproduction, which makes the phylogenetic analysis difficult. On the basis of the previous analysis of the variation of nrDNA in Taraxacum taxa with different ploidy levels and modes of reproduction, to mitigate consequen...

Background / Purpose: We present here data concerning abundance and distribution of cisZ(s) around the plant kingdom, their activities in different bioassays as well as their dynamics during ontogeny and the involvement in plant stress responses with the aim to reveal a potential function and significance of cisZ-types in plant biology. Main co...

The new genus Oreojuncus Záveská Drábková et Kirschner has been described, with two species – O. trifidus and O. monan­thos. Attention is given to the first species, which occurs in the Czech Republic. The genus Oreojuncus has been traditionally included in the Juncus sect. Steirochloa; however, complex research based on phylogenetic analysis of th...

With the expansion of molecular techniques, the historical collections have become widely used. Studying plant DNA using modern molecular techniques such as DNA sequencing plays an important role in understanding evolutionary relationships, identification through DNA barcoding, conservation status, and many other aspects of plant biology. Enormous...

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The order Poales is well known for the presence of holocentric chromosomes, agmatoploidy, symploidy, polyploidy and high variation in chromosome numbers among and within species and genera. The second largest family of this order, Cyperaceae, is very well characterized both karyologicaly and cytologicaly. On the other hand, a smaller family Juncace...

The present study examines chromosome and genome size evolution in Luzula (woodrush; Juncaceae), a monocot genus with holocentric chromosomes. Detailed karyotypes and genome size estimates were obtained for seven Luzula spp., and these were combined with additional data from the literature to enable a comprehensive cytological analysis of the genus...

DNA barcoding is a technique of species identification by using standardized DNA sequence. This technique represents a challenge in the taxonomic approaches avoiding the need of exact knowledge of morphology, particularly when diagnostic morphological features are absent. There has been a considerable debate regarding a locus choice for standard ba...

Screening of nuclear genome size was carried out on ca 2400 plants from over 120 mainly Central- European localities of the Juncus bufonius group. Besides the diploid level, corresponding to known diploid species (in this case J. ranarius, J. hybridus and J. sorrentini), two polyploid cytotypes were detected, conforming with the tetraploid and hexa...

The genus Luzula consists of 115 species distributed throughout the world. Luzula is monophyletic, but species relationships within the genus are difficult to determine primarily due to the similar morphology even within geographically remote taxa (especially within the section Luzula). The plastome trnL intron, trnL-F intergenic spacer and the nuc...

A phylogenetic analysis of the Juncaceae was conducted to assess relationships within the genera Juncus, Luzula and five other small South American genera (Distichia, Marsippospermum, Oxychloë, Patosia and Rostkovia). We examined parallel datasets from organelles (mtDNA: atp1 gene, cpDNA: trnL intron, trnL-F intergenic spacer, rbcL gene) with respe...

Recently, advocates of DNA taxonomy have complained that there is inadequate control of the taxonomy in databases such as GenBank. This is correct, but the uncertainty may be extended to the sequences themselves. The present study shows that as long as vouchers are available neither problem is fatal, but if no voucher exists, bad sequences and bad...

Proposal to conserve the name Juncus micranthus Schrad. ex E. Mey.

Use of molecular data for phylogenetic studies in Juncaceae/Cyperaceae beginning in 1993 has resulted in an uncertain taxonomic position of the Andean Oxychloë Phil. RbcL data used in earlier analyses resolved Oxychloe inside Cyperaceae or as their sister group. However, many typically juncaceous morphological features (e.g., spiro- or ortostichous...

Use of molecular data for phylogenetic studies in Juncaceae/Cyperaceae beginning in 1993 has resulted in an uncertain taxonomic position of the Andean Oxychloë Phil. Based on rbcL data Duvall & al. (1993), Plunkett & al. (1995) and Muasya (1998, 2000) suggested that the Andean Oxychloë was placed inside Cyperaceae or as their sister group, however,...

Juncus alpigenus K. Koch is reported to occur in the province of Azerbaijan, northwestern Iran. The species was previously recorded from the countries of the Caucasus and NE Turkey. The species belongs to Juncus sect. Ozophyllum, the Juncus atratus group, which is characterized by prominent longitudinal ridges on leaves. Relationships of Juncus alp...

Juncus and Luzula are the largest, almost cosmopolitan, genera in the Juncaceae. Relationships within Juncus and Luzula and among other genera of Juncaceae (Distichia, Marsippospermum, Oxychloë, Patosia and Rostkovia) remain incompletely resolved. RbcL sequence data resolved a part of the supraspecific phylogeny, but many clades remain polytomic. F...

TheMelampyrum sylvaticum group is a heterogeneous group at the inter- and infra-specific level. A wide range of morphological characters was examined on specimens collected for this study. The corolla size, the corolla colour and the anther length are considered the most important morphological characters at the interspecific taxonomic level. The m...

The Melampyrum sylvaticum agg. is a heterogeneous group at the inter- and infraspecific level. Several more or less independent trends in the variation of Melampyrum sylvaticum s.l. are conspicuous. Wide range of morphological characters was examined on specimens collected for this study. The corolla size, the corolla colour and the anther length a...

Seven hundred fifty-two to one thousand ninety-seven base pairs of the trnL intron and trnL- trnF intergenic spacer of the chloroplast DNA of 55 Juncaceae taxa (Juncus, Luzula, Rostkovia, and Oxychloë) was sequenced. Seventeen structural mutations (13 indels marked A to M, 3 parts of the trnF pseudogene, and insertion "o" within a pseudogene) withi...