Leila B. Mostaço-Guidolin

Leila B. Mostaço-Guidolin
Carleton University · Department of Systems and Computer Engineering

Ph.D. in Biomedical Engineering

About

37
Publications
10,077
Reads
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531
Citations
Introduction
In the past years, I have been working on developing specific image analysis methods to extract features associated with extracellular matrix (ECM) remodelling, which is a common factor in several diseases. At Carleton, my research platform is combining the use of microscopy (especially nonlinear optical microscopy) and image analysis with 3D bioprinting, aiming to address questions related to cellular communication and repair of the ECM in abnormal mechano-environments.
Additional affiliations
January 2020 - present
Carleton University
Position
  • Professor (Assistant)
September 2015 - December 2019
University of British Columbia - Vancouver
Position
  • PostDoc Position
January 2011 - April 2015
University of Manitoba
Position
  • PhD Student
Education
January 2011 - May 2014
University of Manitoba
Field of study
  • Biomedical Engineering
August 2007 - August 2009
University of São Paulo
Field of study
  • Physics Applied to Medicine and Biology
January 2003 - July 2007
University of São Paulo
Field of study
  • Medical Physics

Publications

Publications (37)
Article
Full-text available
The extracellular matrix (ECM) supports lung tissue architecture and physiology by providing mechanical stability and elastic recoil. Over the last several decades, it has become increasingly clear that the stiffness of the ECM governs many cellular processes, including cell-phenotype and functions during development, healing, and disease. Of all t...
Article
Full-text available
Biological tissues are not uniquely composed of cells. A substantial part of their volume is extracellular space, which is primarily filled by an intricate network of macromolecules constituting the extracellular matrix (ECM). The ECM serves as the scaffolding for tissues and organs throughout the body, playing an essential role in their structural...
Article
Full-text available
Mitochondria are the metabolic hub of the cell, playing a central role in regulating immune responses. Dysfunction of mitochondrial reprogramming can occur during bacterial and viral infections compromising hosts’ immune signaling. Comparative evaluation of these alterations in response to bacterial and viral ligands can provide insights into a cel...
Article
Full-text available
The alveolar ducts are connected to peripheral septal fibers which extend from the visceral pleura into interlobular septa, and are anchored to axial fibers in the small airways. Together these axial and septal fibers constitute a fiber continuum that provides tension and integrity throughout the lung. Building on the observations that alveolar duc...
Article
Full-text available
In asthma, the airway epithelium has an impaired capacity to differentiate and plays a key role in the development of airway inflammation and remodeling through mediator release. The study objective was to investigate the release of (IL)-1 family members from primary airway epithelial-cells during differentiation, and how they affect primary airway...
Article
Background: This study sought to investigate the impact of extensive over-expansion of transcatheter heart valves (THVs) on leaflet structure. THVs may be over-expanded beyond recommended labeling in certain clinical scenarios. However, it is unknown whether over-expansion damages pericardial leaflets. Therefore, we assessed the effect of over-expa...
Article
Polarimetric second-harmonic generation (P-SHG) microscopy is used to quantify the structural alteration of collagen in stage-I,-II and -III non-small cell lung carcinoma (NSCLC) ex vivo tissue. The achiral and chiral molecular second-order susceptibility tensor components ratios (R and C, respectively), the degree of linear polarization (DLP) and...
Article
Full-text available
Multiphoton microscopy is a powerful, non-invasive technique to image biological specimens. One current limitation of multiphoton microscopy is resolution as many of the biological molecules and structures investigated by research groups are similar in size or smaller than the diffraction limit. To date, the combination of multiphoton and super-res...
Article
Full-text available
RATIONALE: Histological stains have been used as the gold standard to visualize extracellular matrix (ECM) changes associated with airway remodeling in asthma, yet they provide no information on the biochemical and structural characteristics of the ECM, which are vital to understanding alterations in tissue function. OBJECTIVE: Demonstrate the use...
Article
Full-text available
Structural proteins like collagen and elastin are major constituents of the extracellular matrix (ECM). ECM degradation and remodeling in diseases significantly impact the microorganization of these structural proteins. Therefore, tracking the changes of collagen and elastin fiber morphological features within ECM impacted by disease progression co...
Article
Full-text available
The ability to respond to injury with tissue repair is a fundamental property of all multicellular organisms. The extracellular matrix (ECM), composed of fibrillar collagens as well as a number of other components is dis-regulated during repair in many organs. In many tissues, scaring results when the balance is lost between ECM synthesis and degra...
Article
Abnormal thickening of the airway basement membrane is one of the hallmarks of airway remodeling in asthma. The present protocols for measuring the basement membrane involve the use of stained tissue sections and measurements of the basement membrane thickness at certain intervals, followed by the calculation of the geometric mean thickness for eac...
Article
Full-text available
Asthma is characterized as a chronic inflammatory airway disease associated with airway wall remodeling. Airway remodeling involves all tissues of the airway wall and specifically leads to alterations in collagen and elastin morphology and distribution compared non-asthmatic airways. Multimodal nonlinear optical (NLO) microscopy, has shown potentia...
Article
Quantification of atherosclerosis has been a challenging task owing to its complex pathology. In this study, a quantitative imaging method for assessing atherosclerosis progression was validated in a rabbit model. This method is based on the calculation of a numerical parameter, optical index for plaque burden or OIPB, which is derived from the non...
Article
Full-text available
In this study we present a novel image analysis methodology to quantify and to classify morphological details in tissue collagen fibril organization and lipid deposition. Co-localized collagen (second harmonic, SHG) and lipid (coherent Raman, CARS) images of atherosclerotic artery walls were acquired by a supercontinuum-powered multi-modal nonlinea...
Article
Full-text available
In this study we present an image analysis methodology capable of quantifying morphological changes in tissue collagen fibril organization caused by pathological conditions. Texture analysis based on first-order statistics (FOS) and second-order statistics such as gray level co-occurrence matrix (GLCM) was explored to extract second-harmonic genera...
Article
Full-text available
Pathological understanding of arterial diseases is mainly attributable to histological observations based on conventional tissue staining protocols. The emerging development of nonlinear optical microscopy (NLOM), particularly in second-harmonic generation, two-photon excited fluorescence and coherent Raman scattering, provides a new venue to visua...
Conference Paper
Full-text available
Nonlinear optical (NLO) microscopy provides a minimally invasive optical method for fast molecular imaging at subcellular resolution with 3D sectioning capability in thick, highly scattering biological tissues. In the current study, we demonstrate the imaging of arterial tissue using a nonlinear optical microscope based on photonic crystal fiber an...
Article
Full-text available
The composition and structure of atherosclerotic lesions can be directly related to the risk they pose to the patient. Multimodal nonlinear optical (NLO) microscopy provides a powerful means to visualize the major extracellular components of the plaque that critically determine its structure. Textural features extracted from NLO images were investi...
Article
Full-text available
Fourier transform infrared (FTIR) spectroscopy is an effective and nondestructive method for monitoring cellular alterations. Combining the advantages of FTIR spectroscopy with the challenge of cellular characterization, the main objective of this review is to collect information related to the spectroscopic identification of blood cells, focusing...
Article
Full-text available
Luminal atherosclerosis imaging was demonstrated by multimodal femtosecond CARS microscopy (MM-CARS). Using a myocardial infarction-prone rabbit model of atherosclerosis, this study demonstrated the utility of multimodal CARS imaging in determining atherosclerotic plaque burden through two types of image analysis procedures. Firstly, multimodal CAR...
Article
Full-text available
A femtosecond CARS-based nonlinear optical microscope was used to simultaneously image extracellular structural proteins and lipid-rich structures within intact aortic tissue obtained from myocardial infarction-prone Watanabe heritable hyperlipidemic rabbits (WHHLMI). Clear differences in the NLO microscopic images were observed between healthy art...
Article
Full-text available
Label-free imaging of bulk arterial tissue is demonstrated using a multimodal nonlinear optical microscope based on a photonic crystal fiber and a single femtosecond oscillator operating at 800 nm. Colocalized imaging of extracellular elastin fibers, fibrillar collagen, and lipid-rich structures within aortic tissue obtained from atherosclerosis-pr...
Article
Full-text available
The present study was designed to identify and compare the infrared absorption spectra of two human breast cancer cell lines: MCF-7 (estrogen receptor expressed, ER+) and SKBr3 (estrogen receptor non-expressed, ER–). Comparison between SKBr3 and MCF-7 cells revealed differences in the following absorption band areas: 1087 cm–1 (DNA), 1397 cm–1 (CH3...
Article
Full-text available
The present study was designed to identify and compare the infrared absorption spectra of two human breast cancer cell lines: MCF-7 (estrogen receptor expressed, ER+) and SKBr3 (estrogen receptor non-expressed, ER–). Comparison between SKBr3 and MCF-7 cells revealed differences in the following absorption band areas: 1087 cm –1 (DNA), 1397 cm –1 (C...
Article
Full-text available
Nonlinear optical (NLO) microscopy is used to follow key structural and biochemical changes associated with the progression of atherosclerosis. Arteries from WHHL-MI rabbits are examined using a 3 channel NLO microscope that can simultaneously monitor the coherent anti-stokes Raman scattered light (CARS), the two-photon excited fluorescence (TPEF)...
Article
Full-text available
The aim of this review is to combine all the information related to the characterization of skin tissues and cells, focusing on the identification of the specific biochemical characteristics of skin cancer. We have characterized two types of melanoma by FTIR spectroscopy: a murine melanoma (B16F10 cell line) and a human melanoma (C8161 cell line)....

Questions

Questions (3)
Question
Hi!
I came across the Nanolive system and I am wondering if anybody here has had a chance to use it. https://www.nanolive.ch/
It seems an interesting simple solution for basic cell imaging, but I am not fully convinced of its capability/versatility.
Any thoughts?
My main application would be a general morphology assessment of fibroblast cells under different chemical/mechanical conditions.
Many thanks in advance for any input.
Question
Hello!
I am in the process of setting up a bioprinting lab (from scratch!) and of course, as a new faculty, I have limited funds.
I am wondering if anyone had any experience with Bio X (Cellink) and/or Aspect Rx1?
I would be looking into printing alginate and lots of collagen-based materials (with likely just one cell line in there).
Any thoughts/advice/recommendations?
I wonder if the extrusion method used on the Bio X (syringe extruders) might be better/worse than the RX1(microfluidic chips). I know that the RX1 is likely much more expensive than the Bio X; however, if that would mean fewer headaches down the road, it might be worth it..?
Many thanks in advance!
Question
Hello! We are working with human lung tissue, histology sections and would like to image them with FTIR and Raman.
However, after exhaustive research I have seen papers talking about significant changes in the collagen secondary structure when fixing the samples with ethanol. However, others report just slightly peak shifts of 1-2 cm-1 (when looking at protein bands).
Our main interest is knowing the spacial distribution of different collagen types on the tissue (at this point, we don't care about quantification and lipid content).
Thanks a lot! :)

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