Leila Hambaba

Leila Hambaba
Université Batna 2 · Microbiology and Biochemistry

Professor

About

122
Publications
50,046
Reads
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426
Citations
Citations since 2017
56 Research Items
334 Citations
2017201820192020202120222023020406080100120
2017201820192020202120222023020406080100120
2017201820192020202120222023020406080100120
2017201820192020202120222023020406080100120
Introduction
Hambaba Leila currently works as Professor and member in a laboratory of the Department of Microbiology and Biochemistry University of Batna 2, Algeria. She is currently interested in Biochemistry, phytochemistry and pharmacology.

Publications

Publications (122)
Article
Full-text available
The gastric pentagastrin-stimulated secretions of acid (peak acid output) and of unsaturated intrinsic factor in eight cystic fibrosis patients (1.4 +/- 0.5 mEq/kg/h and 0.27 +/- 0.12 nmol/kg/h, respectively) were significantly enhanced (p less than 0.05) when compared with six normal controls (0.27 +/- 0.16 mEq/kg/h and 0.10 +/- 0.02 nmol/kg/h, re...
Article
The authors hypothesized the possibility of quantitative or qualitative abnormalities of amniotic fluid B12-binding proteins in cystic fibrosis. Seven cystic fibrosis and 59 normal amniotic fluids sampled between 17.5 and 18.5 gestational weeks were studied by radioimmunogel filtration, radioisotope technique, and isoelectric focusing chromatograph...
Article
Indirect evidence for the presence of intrinsic factor in amniotic fluid has been provided recently, using either a radioisotope binding assay or a radioimmunoassay. We have determined the unsaturated cobalamin binding capacity and the physicochemical properties of the 3 cobalamin binding proteins in 59 amniotic fluids using radioisotope binding as...
Article
Plasmatic homocysteine concentration depends mostly on 5,10 methylene tetrahydrofolate reductase (MTHFR) polymorphisms, a key enzyme in folate metabolism. The most common point mutation C677T is associated to cardiovascular and neurological pathologies; its ethnic repartition is quite heterogenic. In the present study, we proposed to describe the g...
Article
Bioinformatics is inherently an innovative field that is situated at the limit of life and computer sciences that allowed new technological advances in genome sequencing, data processing, predication and simplified the treatment of complex and huge data. This field is related on two common approaches namely; in silico and molecular docking-dynamic...
Article
Full-text available
Among the Mediterranean aromatic plants, Ruta tuberculata Forssk. (Rutaceae) has been widely used as a traditional natural remedy against various disorders resulting from its divers’ pharmacological virtues. The aim of this study is to characterize for the first time the phenolic profile of its ethyl acetate (EtOAcE) and acetonic (AcE) extracts and...
Book
La biotechnologie consiste à comprendre le fonctionnement des organismes vivants au niveau moléculaire. Dans sa forme la plus simple, la biotechnologie est une technologie basée sur la biologie, exploitant les processus cellulaires et biomoléculaires pour développer des technologies et des produits qui contribuent à améliorer nos vies et la santé d...
Article
Full-text available
Background and Objective: Phytotherapy is considered nowadays an important discipline that allowed both the scientific community and pharmaceutical companies to innovate in the drug discovery and development process. In this study, we aimed to evaluate the anti-inflammatory activity of the n-butanol (n-BuOH) and ethyl acetate (EA) extracts of a pla...
Article
Full-text available
The perennial aromatic plant Ruta tuberculata Forssk (Rutaceae) has been traditionally used by Mediterranean peoples as folk medicine against several types of disease to treat diverse illness. The objective of this work is to evaluate the in vitro and in vivo pharmacological activities of the aqueous (RAE) and methanolic (MeOH) 80% (RME) extracts o...
Article
Full-text available
Plants are considered an inestimable source of therapy, and one of the oldest. The structural elucidation of phytocompounds allowed the scientific community to elaborate on new effective drugs, which has considerably enriched the international pharmacopoeia and allowed society to overcome several epidemic and pandemic situations. Centaurea species...
Article
Full-text available
Bioinformatics as a newly emerging discipline is considered nowadays a reference to characterize the physicochemical and pharmacological properties of the actual biocompounds contained in plants, which has helped the pharmaceutical industry a lot in the drug development process. In this study, a bioinformatics approach known as in silico was perfor...
Book
Considered as the gateway between Africa and Europe, Algeria is one of the largest and most beautiful countries in north africa, with a georgraphic diversity extending from the northernmost, which is generally known as the Tell, and subjected to the moderating influences of the Mediterranean and consists largely of the Atlas Mountains, which separa...
Article
Full-text available
Nowadays, plants bioactive compounds are considered as a new source of therapy, especially for the elaboration process of more effective drugs. 80% of the actual drug substances are purely natural and originate from plants, representing a new hope, especially for the treatment of chronic illnesses. The aim of this study is to characterize the phyto...
Book
Nowadays, phytotherapy is considered by many scientists as the ancestor and the basis of the actual medicine. This old discipline rely in the pharmacological properties of plants to treat various diseases and conditions and supported human beings in all aspect throughout their evolution which allowed several civilizations to develop several remedie...
Article
Full-text available
Centaurea species are widely used in traditional medicine to treat several illnesses, especially by Mediterranean populations due to their pharmacological properties. The present study aimed to evaluate for the first time some in vivo activities of n-butanol (n-BuOH) extract of the aerial part of Centaurea tougourensis. For this approach; the antid...
Article
Full-text available
Introduction Traditional medicine has an important place in human history and this since antiquity. Indeed, during Egyptian and Chinese civilization era, many detailed manuscripts, describing the therapeutic effect of plants, were found which suggest that folk medicine is the basis of the actual medicine. Objective To investigate the phytochemical...
Article
Objective Ruta tuberculata forssk. (Rutaceae) is an aromatic plants widely used in Algerian traditionally medicine due to its pharmaceutical virtues against various disorders. This study aims to determine the phenolic profile of aqueous (RAE) and methanol (RME) extracts of R. tuberculata aerial parts and to investigate their acute oral toxicity, as...
Article
Marrubium vulgare L. and Marrubium deserti de Noé have been widely used by the local population for wound healing and disinfection. The arial parts of both species were reported to relieve pain and inflammation. However, insufficient data in the literature supports the traditional use of these species. For this, the present study investigated the i...
Article
Full-text available
Purpose: To determine the anti-inflammatory and anti-ulcer properties of the aerial parts of Centaurea tougourensis Boiss. & Reut. Methods: The effects of n-butanol (n-BuOH) extract of the aerial part of Centaurea tougourensis on carrageenan-induced paw edema and ethanol-induced gastric mucosal damage were determined at 2 doses (200 and 400 mg/kg,...
Presentation
More than 500 Centaurea species compose the Asteraceae family, and most of the recent studies made on the species of this genus proved their pharmacological potential, especially to treat chronic illnesses. Aims: To evaluate for the first time the antioxidant, anti-inflammatory, neuroprotective and anti-microbial properties of the n-butanol (n-BuOH...
Article
Full-text available
Context: More than 500 Centaurea species compose the Asteraceae family, and most of the recent studies made on the species of this genus proved their pharmacological potential, especially to treat chronic illnesses. Aims: To evaluate for the first time the antioxidant, anti-inflammatory, neuroprotective and anti-microbial properties of the n-butan...
Article
Full-text available
The objective of the present study was to determine the pharmacological properties of the methanolic (MeOH) extract of Marrubium deserti leaves. For this purpose, antioxidant activity was carried out by DPPH and Ferric reducing power (FRAP) assays respectively while In vivo anti-inflammatory activity was tested by carrageenan-induced paw edema mode...
Conference Paper
Full-text available
In the last decade, the number of diabetic persons has considerably increased and the scientific community approach to understand this complex pathology has become very difficult due to the fact that diabetes is linked to other illnesses which can generate serious complications like glaucoma, nerve damage. Plants are considered new alternative reso...
Presentation
In the last decade, the number of diabetic persons has considerably increased and the scientific community approach to understand this complex pathology has become very difficult due to the fact that diabetes is linked to other illnesses which can generate serious complications like glaucoma, nerve damage. Plants are considered new alternative reso...
Article
Full-text available
Hashimoto’s thyroiditis (HT) is a chronic autoimmune disease of the thyroid gland and is also the main cause of hypothyroidism. A recent genome-wide association study (GWAS) suggested an association of three novel genetic variants with HT in a population of Caucasian origin (Croatian). A case-control study was performed to investigate the associati...
Article
The novel coronavirus outbreak (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) represents the actual greatest global public health crisis. The lack of efficacious drugs and vaccines against this viral infection created a challenge for scientific researchers in order to find effective solutions. One of the promising...
Article
The novel coronavirus outbreak (COVID-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) represents the actual greatest global public health crisis. The lack of efficacious drugs and vaccines against this viral infection created a challenge for scientific researchers in order to find effective solutions. One of the promising...
Article
The biological struggle by the use of the natural substances that have a pesticide effect may be an alternative to harmful chemicals; among these natural substances are the extracts of aromatic plants especially essential oils. The present work aims to highlight the fungicidal and insecticidal effect of the essential oil (EO), of an aromatic plant...
Article
Dans la présente étude, in vitro, l’activité antimicrobienne et le pouvoir antioxydant des différents extraits préparés à partir des feuilles du Marrubium deserti de Noé ont été étudiés. L’activité antimicrobienne a été évaluée par la méthode de diffusion en milieu gélosé contre Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), Pse...
Article
The present study investigates the evaluation of in vitro pharmacological activities of crude extracts (petroleum ether, ethyl acetate and n-butanol) obtained from the plant Scabiosa stellata L. The cytotoxicity of extracts was tested by Brine shrimp lethality method; the acetylcholinesterase inhibitory activity was performed using Ellman's colorim...
Conference Paper
Effect of pH and temperature on polyphenoloxidase and peroxidase activities was studied to highlight the enzymatic browning which limits the conservation of Deglet Nour and Ghars dates by leading to the loss of their nutritional quality. Stability rates at wide ranges of pH (2.4 - 8.6) and temperatures (10 - 90 °C) were assessed by assaying the res...
Article
This study is devoted to the estimation of total phenolic content and the evaluation of in vitro antioxidant and antibacterial activities of crude extracts (petroleum ether, ethyl acetate and methanolic) obtained from the species Silene inflata Sm. The total phenolic content of the obtained extracts was estimated spectrophotometrically by the Folin...
Article
This study is devoted to the estimation of total phenolic content and the evaluation of in vitro antioxidant and antibacterial activities of crude extracts (petroleum ether, ethyl acetate and methanolic) obtained from the species Silene inflata Sm. The total phenolic content of the obtained extracts was estimated spectrophotometrically by the F...
Article
Full-text available
Objective In this study, cytotoxic effect, anticholinesterase, hemolytic and antibacterial activities of crude extracts (petroleum ether, ethyl acetate and n-butanol) obtained from the plant Scabiosa stellata L. were evaluated. Methods The cytotoxicity of extracts was tested by Brine shrimp lethality method; the acetylcholinesterase inhibitory act...
Article
Full-text available
This study is devoted to the estimation of total bioactive contents and the evaluation of acute toxicity and in vivo anti-inflammatory effects and the assessment of in vitro antioxidant and anti-arthritic potential of the species Scabiosa stellata. The anti-arthritic activity was performed by bovine serum albumin denaturation method and the antioxi...
Conference Paper
Anibiotic resistance is a major global problem and there is a pressing need to develop new therapeutic agents. In this context, the antibacterial potentials of two popular medicinal plants ( Marrubium vulgare and Marrubium deserti) were evaluated against eight bacterial strains: Escherichia Coli ATCC 259232, Pseudomonas aeruginosa ATCC 27853, Staph...
Article
The first biological study of Cardopatium corymbosum L. consists of analyzing qualitatively and quantitatively the methanolic, dichloromethanic, and etheropetrolic extracts from aerial part and investigating the antibacterial and haemostatic activities. The qualitative analyses of these extracts by thin layer chromatography (TLC) reveal the probabl...
Presentation
Medicinal plants can be an important source of new biochemical molecules with potential therapeutic effects because they have the ability to treat inflammatory and infectious diseases. In this context, we are interested in the biochemical study of the plant Scabiosastellata L. belonging to the family Dipsacaceae and the evaluation of its biological...
Presentation
Medicinal plants can be an important source of new biochemical molecules with potential therapeutic effects because they have the ability to treat inflammatory and infectious diseases. In this context, we are interested in the biochemical study of the plant Scabiosastellata L. belonging to the family Dipsacaceae and the evaluation of its biological...
Presentation
Biochemical Composition And Biological Activities Of The Species Scabiosa stellata.
Poster
The present study was designed to explore the anti-inflammatory effect and antioxidant potential of the methanolic extract of Marrubium vulgare leaves, a popular traditional medicinal herb. In vivo anti-inflammatory activity was evaluated by the paw edema assay induced by Carrageenin in rat model, while antioxidant activity was evaluated by two tes...
Poster
the organizing committee of the 4th International Congress of Plants Biodiversity
Article
Full-text available
The first biological study of Cardopatium corymbosum L. consists of analyzing qualitatively and quantitatively the methanolic, dichloromethanic, and etheropetrolic extracts from aerial part and investigating the antibacterial and haemostatic activities. The qualitative analyses of these extracts by thin layer chromatography (TLC) reveal the probabl...
Poster
Les espèces réactives de l’oxygène (ROS) sont des composés toxique à presque tout type de constituants cellulaires qui cause de graves défaillances métabolique et endommage les macromolécules biologiques, elles sont impliquées dans l’induction et/ou l’augmentation d’un certain nombre de pathologies humaines tel que: le cancer, les troubles cardiova...
Poster
Background and aim: the aerial parts of Urtica pilulifera L. ((Urticaceae), El-Hourreigue (in Arabic), have been used in traditional Algerian medicine and in the most Mediterranean areas for their therapeutic properties. However, it is unclear which components in the leaves of this plant are effective for the treatment of diseases related to oxidat...
Article
Full-text available
The present study was designed to explore the anti-inflammatory effect and antioxidant potential of the methanolic extract of Marrubium vulgare leaves, a popular traditional medicinal herb. In vivo anti-inflammatory activity was evaluated by the paw edema assay induced by Carrageenin in rat model, while antioxidant activity was evaluated by two tes...
Article
The influence of pH, temperature and some chemicals on polyphenoloxidase and peroxidase activities in harvested Deglet Nour and Ghars dates was studied. These activities were tested for optimum pH and temperatures. Their stability was assessed as a percentage of remaining activity after incubation at different pH and temperatures and in the presenc...
Poster
2nd Euro Mediterranean Symposium on Fruit and Vegetable processing. Avignon- Paris,
Conference Paper
The overall objective of this study was to evaluate in vitro the hemostatic activity of secondary metabolites (polyphenols, flavonoids and tannins) of Marrubium vulgare leaves, aromatic plant widely used in traditional medicine for the treatment of asthma, cough diabetes (by its effect on the pancreas to secrete insulin), heart disease, fever has a...
Article
This work aims to investigate the acute toxicity of methanolic extract (MeOHE) from Marrubium deserti leaves and evaluate their in vivo anti-inflammatory and analgesic properties. Quantitative determinations of total polyphenols and flavonoïds revealed that MeOHE is rich in polyphenols (184 ± 0.78 mg GAE/mg of extract) and flavonoïds (28.48 ± 0.40...
Article
Full-text available
This work aims to investigate the acute toxicity of methanolic extract (MeOHE) from Marrubium deserti leaves and evaluate their in vivo anti-inflammatory and analgesic properties. Quantitative determinations of total polyphenols and flavonoïds revealed that MeOHE is rich in polyphenols (184 ± 0.78 mg GAE/mg of extract) and flavonoïds (28.48 ± 0.40...
Article
Full-text available
Dans la présente étude, in vitro, l’activité antimicrobienne et le pouvoir antioxydant des différents extraits préparés à partir des feuilles du Marrubium deserti de Noé ont été étudiés. L’activité antimicrobienne a été évaluée par la méthode de diffusion en milieu gélosé contre Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), Pse...
Article
L'objectif de cette étude est d’évaluer in vitro l’activité hémostatique des métabolites secondaires des feuilles de Marrubium vulgare. L’analyse qualitative de l’extrait aqueux (EAQ) par la chromatographie sur couche mince a révélé la présence de la quercétine, la rutine et le kaempférol. La quantification des phénols totaux par la méthode de Foli...
Article
In the current in vitro study, antioxidant and antimicrobial activities of the organic solvents and aqueous extracts of the leaves and flowered tops of Marrubium vulgare were investigated. Antioxidant activity was evaluated by 2,2’-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity and β-carotene bleaching method, and the antimicrobial ac...
Article
Full-text available
La datte (Phoenix dactylifera L.) possède maintes vertus thérapeutiques qui nécessitent des études plus approfondies sur ses composants. En effet, les composés phénoliques sont des métabolites secondaires de grande importance pharmacologique, vu leurs diverses activités biologiques confirmées dans un grand nombre d’études. C’est dans ce contexte qu...
Conference Paper
Le palmier dattier ( phoenix dactyliféra L.) est la plus importante culture des zones arides et semi-arides. Il joue un rôle important dans la vie économique et sociale des populations de ces régions. Son fruit est la datte qui est en plus de son usage diététique, occupe une bonne place dans la médecine traditionnelle. Par ailleurs, les différentes...
Article
The date fruit (Phoenix dactylifera L.) possesses a number of therapeutic virtues, and this requires more studies on its components. Phenolics are secondary metabolites of great pharmacological importance because of their several biological activities confirmed in a great number of studies. It’s in this context that we carried out a study with the...
Conference Paper
In Algeria, some fruit trees produce fruits in free nature. Such trees are Celtis australis, Crataegus azarolus, Crataegus monogyna and Zizyphus lotus. In spite of their appreciable consumption, their nutritional value remains unknown. The objective of this study is the determination of sugars in the pulpe and almond of the above fruits. The bioche...