Leif Salford

Leif Salford
Lund University | LU · Dept of Neurosurgery, The Rausing Laboratory

MD, PhD

About

338
Publications
25,179
Reads
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7,552
Citations
Additional affiliations
August 1977 - February 2016
Lund University
Position
  • Professor of Neurosurgery and Laboratory Director

Publications

Publications (338)
Article
Full-text available
Chronic inflammatory diseases like rheumatoid arthritis are characterized by a deficit in fully functional regulatory T cells. DNA-methylation inhibitors have previously been shown to promote regulatory T cell responses and, in the present study, we evaluated their potential to ameliorate chronic and acute animal models of rheumatoid arthritis. Of...
Article
Full-text available
The aim was to study the level of S-100 in blood samples taken from Fischer-344 rats after exposure to 915MHz CW-microwaves and ELF magnetic fields in TEM-cells. Magnetic field exposure took place with the TEM cell in a Helmholtz coil arrangement of either 50 Hz sinusoidal magnetic field of 5µT, or incoherent magnetic field noise IMF at a maximum a...
Article
Full-text available
The present study aims to find out if the level of S-100 in blood samples taken from Fischer-344 rats exposed in TEM-cells to microwaves at the 900 MHz band at various modulation frequencies 4, 8.33, 16, 50 Hz and 217 Hz with various peak power output, correlate with albumin leakage through the Blood-Brain Barrier, The results of the present study...
Article
Full-text available
Background Glioblastoma is the most common primary malignant brain tumor in adults. Previous studies have suggested that CRP (C-reactive protein) could serve as a biomarker candidate as well as a prognostic factor in glioblastoma patients, and we here further investigate its potential role. Materials and methods Publicly available datasets were us...
Article
Full-text available
Glioblastoma multiforme (GBM) is an aggressive primary brain malignancy with a very poor prognosis. Researchers employ animal models to develop potential therapies. It is important that these models have clinical relevance. This means that old models, propagated for decades in cultures, should be questioned. Parameters to be evaluated include wheth...
Article
Purpose: The complement system has recently sparked more interest in cancer research. The classical pathway is initiated by activation of the C1 complex, which irreversibly can be bound to and inhibited by C1-INH. We have previously shown that C1-INH is upregulated in human glioblastoma (astrocytoma grade IV) on both gene and protein level. We her...
Article
Purpose: Glioblastoma multiforme (GBM) or astrocytoma grade IV is the most common type of primary brain tumor in adults. In the present study, we investigate the role of the complement system in the glioblastoma situation in an experimental model, since we have previously been able to show a blockade of this system in the glioblastoma setting. Tech...
Article
Full-text available
Glioblastoma multiforme (GBM) is the most common malignant primary brain tumor, and available experimental and routine therapies result in limited survival benefits. A vulnerability of GBM cells to catastrophic vacuolization and cell death, a process termed methuosis, induced by Vacquinol-1 (VQ-1) has been described earlier. In the present study, w...
Article
A major problem regarding glioblastomas is the capacity of glioma cells to inactivate the body’s immune response. The complement system acts as a functional bridge between the innate and adaptive immune response. Previously, it was thought that the complement system was upregulated in glioblastomas. However, only downstream components were studied....
Article
In a previous study, we have found a synergistic effect of indoleamine-2,3-dioxygenase (IDO1)-inhibition using 1-DL-methyl-tryptophan (1-MT) in conjunction with single fraction radiotherapy (RT) for rat glioblastoma (GBM). Using an adaptation of a mathematical model describing synergistic effects of immunotherapy and RT by Serre et al, that is taki...
Article
Full-text available
Background Glioblastoma is the most common and aggressive type of primary brain tumor in adults. A key problem is the capacity of glioma cells to inactivate the body’s immune response. The complement system acts as a functional bridge between the innate and adaptive immune response. Still, the role of the complement system has almost been forgotten...
Data
The NS1 tumor model, showing the NS1 tumor in fluorescent microscope after inoculation into a syngeneic Fischer 344 rat. (TIF)
Data
Protein structure for C1-IA in humans and rats. (DOCX)
Data
Schematic drawing of the complement reaction, showing (left) the effect of C1-IA and (right) re-establishing the reaction by anti-C1-IA treatment. (TIF)
Conference Paper
Background Dysfunction of Tregs results in a breakdown of immunological tolerance and has been implicated in the pathogenesis of autoimmune diseases, including systemic lupus erythematosus, rheumatoid arthritis (RA) and type 1 diabetes. Treg function is regulated by epigenetic factors and we have previously reported the presence of Tregs expressing...
Article
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Background: Inositol trispyrophosphate (ITPP) has been shown to reduce tumour growth in different animal cancer models, as well as of human U87 glioma cells grafted onto chick chorioallantoic membrane (CAM). The aim of this study was to establish whether ITPP crosses the blood-brain barrier and whether it halts the growth of RG2 glioblastoma tumou...
Article
Full-text available
BACKGROUND: Many studies have been performed in animal models in the search for possible treatment alternatives against malignant astrocytomas, including glioblastoma multiforme. One of the key problems when treating patients with malignant gliomas is that despite being able to remove the major bulk of the tumour, we know that tumour cells have alr...
Article
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Investigations on exposure to electromagnetic have generated conflicting results both in epidemiological and laboratory studies, leaving their possible health consequences largely inconclusive. One of the well-reported reasons for the discrepancies is that there is no generally accepted theory to describe the interactions between the very weak elec...
Article
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2014). Immunization with syngeneic interferon-gamma (IFN-gamma) secreting tumour cells enhance the Therapeutic effect and Abscopal effect from combined treatment of subcutaneously implanted contra-lateral N29 tumours on Fischer rats with pulsed electric fields (PEF) and 60Co-gamma radiation., Summary The aim of the present study is to study the Abs...
Article
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ABSTRACT In 1996 there was no convincing laboratory evidence that EMFs used in wireless communication could cause tumour promotion at non-thermal exposure levels. Therefore we then performed a study of the effects from exposure to such electromagnetic fields in the rat brain glioma model we were using in our research for brain tumour therapy. By st...
Article
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We hypothesized that key signaling pathways of glioma genesis might enable the molecular classification of gliomas. Gene coexpression modules around epidermal growth factor receptor (EGFR) (EM, 29 genes) or platelet derived growth factor receptor A (PDGFRA) (PM, 40 genes) in gliomas were identified. Based on EM and PM expression signatures, nonnega...
Article
Full-text available
Increased PDGFRA signaling is an essential pathogenic factor in many subtypes of gliomas. In this context the cell surface expression of PDGFRA is an important determinant of ligand sensing in the glioma microenvironment. However, the regulation of spatial distribution of PDGFRA in glioma cells remains poorly characterized. Here, we report that cel...
Article
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Coping with the immune rejection of allotransplants or autologous cells in patients with an active sensitization towards their autoantigens and autoimmunity presently necessitates life-long immune suppressive therapy acting on the immune system as a whole, which makes the patients vulnerable to infections and increases their risk of developing canc...
Article
Full-text available
Signaling of platelet derived growth factor receptor alpha (PDGFRA) is critically involved in the development of gliomas. However, the clinical relevance of PDGFRA expression in glioma subtypes and the mechanisms of PDGFRA expression in gliomas have been controversial. Under the supervision of morphological diagnosis, analysis of the GSE16011 and t...
Data
Selection of glioma-relevant gliogenic genes. A combined glial genesis gene list (172 genes) was created by combining the glial genesis gene list GO:0042063 and the gene list composed of genes controlling oligodendrogenesis [30]. Using unsupervised hierarchical clustering analysis of the expression of glial genesis genes and glioma samples in GSE42...
Data
Differential expression of PDGFRA in gliomas of the same malignancy grade is associated with patient survival time. High-grade (grade III and IV) and low-grade (grade II) gliomas in the Rembrandt data set were separately analyzed for the extent of PDGFRA expression. Kaplan-Meier plots for the survival time of the patients with high-grade gliomas (p...
Article
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Abstract. The purpose of the study was to examine the possibilities of prolonging the life of rats with brain tumours using electroporation only while conducting impedance scans to evaluate the rate of electroporation. During the experiments, the treated rats in the first batch had tumors grow for 14 days and were then given electroporation with 8...
Article
Full-text available
Abstract. In order to analyze the relaxation characteristics in fresh human brain tissue and blood samples we studied the proton relaxation rate dispersion in the frequency interval from 0.01 to 10 MHz. With the Field cycling method we apply, the sample is first magnetized in a relatively high magnetic fields (0.5 Tesla), which then by electronic m...
Article
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Abstract. In previous work we have studied the effects of electromagnetic fields upon the promotion of growth of tumours from two different rat glioma cell lines, RG2 and N32, inoculated in the brains of Fischer 344 rats. No promotions of tumour growth due to the EMF could be demonstrated in large series of animals as compared to their matched cont...
Article
Full-text available
Abstract. Effect of 915 MHz electromagnetic fields (EMF) on the blood brain-barrier (BBB) permeability has been studied in Fischer 344 rats of both sexes. Male and female Fischer 344 rats were exposed in a Transverse Electromagnetic Transmission line chamber to microwaves of 915 MHz as continuous wave (CW) and pulse-modulated with different pulse p...
Article
Full-text available
STUDIES OF EFFECTS OF GSM-900 MICROWAVE EXPOSURE ON DNA ”MICRONUCLEUS” FORMATION IN MICE Possible genotoxic effects of microwave exposure from GSM-900 mobile telephones have investigated with in vivo micronucleus assay of mouse erythrocytes from CBA mice and GFAP knockout mice. No significant change in the frequency of erythrocytes with micronuclei...
Article
Full-text available
Treatment of tumour cell with 5-aza-2-deoxycytidine (DAC) for immune tumour therapy of Glioma in Fischer 344 rats, Acta Scientiarum Lundensia, Vol. 2012-010, pp. 1-21 ISSN 1651-5013 Fisher 344 rats with implanted N29 glioma tumours were treated with Pulsed Electric Fields (PEF) in combination with immunization using either IFN- gene-transfected sy...
Article
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75 RG2 glioma-carrying Fischer rats were treated by photon activation therapy (PAT) with monochromatic synchrotron radiation and stable thallium. Three groups were treated with thallium in combination with radiation at different energy; immediately below and above the thallium K-edge, and at 50 keV. Three control groups were given irradiation only,...
Article
Full-text available
The question whether very weak, low frequency magnetic fields can affect biological matter is still under debate. The theoretical possibility of such an interaction is often questioned and the site of interaction in the cell is unknown. In the present study, the influence of extremely weak 60 Hz magnetic fields on the transport of Ca(2+) was studie...
Conference Paper
Full-text available
Fisher 344 rats implanted with either N29 or N32 glioma tumours were immunized with either IFN- gene-transfected syngeneic tumour cells or IFN- transfected syngeneic cells treated with 10 M 5-aza-2-deoxycytidine (DAC). Separate groups of rats were also treated in combination with Pulsed Electric Fields (PEF) or Radiation therapy. Tumours (N29) w...
Article
Full-text available
The proton relaxation rate dispersion of paramagnetic ions in blood samples was studied in the frequency interval from 10 kHz to 10 MHz. With the Field cycling method we applied, the samples were first magnetized by a relatively high magnetic field (0.5 T). By electronic means this field was rapidly (1 ms) reduced to a lower value in the interval 0...
Article
Full-text available
The tumor growth rate response was studied on N29 rat glioma tumor cells subcutaneously implanted on both hind legs of Fischer-344 rats. At around 30 days after inoculation, RT was given with 60Co gamma radiation with 4 daily fractions of 5 Gy only to the right-lateral tumors. At days 26, 42, and 54 after inoculation, immunization was performed wit...
Article
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To investigate whether mobile phone radiation might affect snail nociception, employing radiofrequency (RF) electromagnetic fields (EMF) which, to our knowledge, have hitherto not been studied in a snail model. Exposure to extremely low frequency (ELF) magnetic fields has however been shown to significantly affect nociceptive responses. In the pres...
Chapter
Although neurosurgery has a long history it was thanks to brave pioneering neurosurgeons such as Harvey Cushing in the United States — and in Sweden Herbert Olivecrona — that the speciality made huge progress during the first half of the 20th century. However, around 1950, the possibility to reveal pathological processes taking place inside the clo...
Data
Full-text available
Radiation Modulated Immune-Response and Abscopal Effect in Rats with Contra-lateral tumours. Bertil R.R. Persson,1,4 Catrin Bauréus Koch,1,4 Gustav Grafström,1,4 Per Munck af Rosenschöld,1 Crister Ceberg,1,4 Henrietta Nittby,2,4 Bengt Widegren3,4 and Leif G. Salford2,4 1Dept. Medical Radiation Physics, 2Dept of Neurosurgery, 3Dept of Tumour Immunol...