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Introduction
Publications
Publications (24)
In Arabidopis a high number of distinct mRNAs move from shoot to root. We previously reported on the correlation of m ⁵ C-methylation and lack of mRNA transport in juvenile plants depending on the RNA methyltransferases DNMT2 NSUN2B . However, to our surprise we uncovered that lack of DNMT2 NSUN2B (writer) activity did not abolished transport of TC...
The HSC70/HSP70 family of heat shock proteins are evolutionarily conserved chaperones involved in protein folding, protein transport, and RNA binding. Arabidopsis HSC70 chaperones are thought to act as housekeeping chaperones and as such are involved in many growth‐related pathways. Whether Arabidopsis HSC70 binds RNA and whether this interaction i...
Generation of stable gene-edited plant lines using clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated protein 9 (Cas9) requires a lengthy process of outcrossing to eliminate CRISPR–Cas9-associated sequences and produce transgene-free lines. We have addressed this issue by designing fusions of Cas9 and guide RNA tra...
Heat shock proteins of the HSC70/HSP70 family are evolutionarily conserved chaperones that are involved in protein folding, protein transport and RNA binding. Arabidopsis HSC70 chaperones are thought to act as housekeeping chaperones and as such are involved in many growth-related pathways. Whether Arabidopsis HSC70 binds RNA and its function has r...
In plants, transcripts move to distant body parts to potentially act as systemic signals regulating development and growth. Thousands of messenger RNAs (mRNAs) are transported across graft junctions via the phloem to distinct plant parts. Little is known regarding features, structural motifs, and potential base modifications of transported transcri...
Nature Plants 1 , 15025 (2015); published 23 March 2015; corrected 21 November 2016. In the version of this Article originally published, the x -axis scale in Fig. 4b was incorrectly labelled and should have ranged from −10 to 10.
In plants, protein-coding messenger RNAs (mRNAs) can move via the phloem vasculature to distant tissues, where they may act as non-cell-autonomous signals. Emerging work has identified many phloem-mobile mRNAs, but little is known regarding RNA motifs triggering mobility, the extent of mRNA transport, and the potential of transported mRNAs to be tr...
The concept that proteins and small RNAs can move to and function in distant body parts is well established. However, non-cell-autonomy of small RNA molecules raises the question: To what extent are protein-coding messenger RNAs (mRNAs) exchanged between tissues in plants? Here we report the comprehensive identification of 2,006 genes producing mob...
Seedling establishment is a critical phase in the life of plants when they are the most vulnerable to environment. Growth arrest at post-germinative stage under stress is the major adaptive strategy to help germinating seedlings to survive a spectrum of stressful conditions. ABA signaling is the key pathway to control stress-induced developmental a...
Post-geminative developmental arrest of 35S:: miR172b on ABA was restored after removing ABA. Seeds of 35::miR172b and Col-0 were germinated on MS medium containing 0.8 µM ABA, the seedlings which turned green were counted at 12 days after stratification (A); the arrested seedlings were transferred onto MS medium (B) or MS plus 0.8 µM ABA (C) for a...
Primers pairs used for construction and genotyping, probes used for EMSA (Sequence 5′→3′).
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Phenotype of 35::miR172b transgenic plants and snz-1 under LiCl and KCl treatment. (A) KCl and LiCl dose–response analysis of seed germination for Col-0, 35S:: miR172b and snz-1 at 3 days after stratification. (B) and (C) Greening of 35:: miR172b transgenic plants and snz-1 was not affected under KCl and LiCl treatment. Photographs were taken 7 day...
SNZ regulates post-germinative developmental arrest induced by abiotic stress through the ABA dependent pathway.
SNZ cDNA under control of 35S promoter was expressed in snz-1 mutant, and the transgenic lines were characterized and used for phenotypic analysis. (A) Transcript abundance of SNZ in wild-type, snz-1 and 35S:: SNZ/snz-1 transgenic lines...
Genotyping of snz-1/abi5-8. (A) Schematics showing the location of the T-DNA insertion in abi5-8(SALK_013163) and snz-1(SALK_030031). Black boxes indicate exons, green box indicate 5′UTR, yellow box indicates 3′UTR, and the horizontal lines indicate introns. (B) Confirmation of the homologous T-DNA insertion in snz-1/abi5-8 by PCR. Col-0 was used a...
cis-acting regulatory elements analysis of the SNZ promoter sequence by PLACE and AtcisDB.
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SNZ and TOE3 were up-regulated by ABA and osmotic stress/drought. Plant materials from 7 and 18 day old wild-type Col-0 was treated by ABA and osmotic stress, respectively. (A) and (B) Expression of SNZ (A) and TOE3 (B) in response to ABA and osmotic stress/drought; The colors from yellow to red indicate the increased absolute signal values of SNZ...
Expression of SNZ and TOE3 in response to ABA. (A) Expression analysis of TOE3 in response to ABA. Wild-type seeds were germinated on MS with or without 5 µM, and materials were collected at 2 to 4 days after stratification. (B) SNZ expression in wild-type (Col-0) and 35S:: miR172b plants on day 3 and 4 after stratification with or without 0.4 µM A...
Primers pairs used for real-time RT-PCR (Sequence 5′→3′).
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Information of the plant materials used in this work.
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Phenotypic analysis of young seedlings of 35S::miR172b and Col-0 in response to ABA and NaCl. The seeds of 35S::miR172b and Col-0 were germinated on MS medium for 5 days and then transferred to MS medium containing NaCl (0, 50, 100 and 150 mM) or ABA (0, 50 and 100 µM). The photographs were taken 7 days after transfer to NaCl (A), and the primary r...
cis-acting regulatory elements analysis of promoter sequence of miR172b by PLACE and PROMO.
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cis-acting regulatory elements RAV1 analysis of promoter sequence of ABA response genes by PLACE and AtcisDB.
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Control of organ growth by coordinating cell proliferation and differentiation is a fundamental developmental process. In plants, postembryonic root growth is sustained by the root meristem. For maintenance of root meristem size, the rate of cell differentiation must equal the rate of cell division. Cytokinin and auxin interact to affect the cell p...
