Lei Fang

Lei Fang
Universitätsspital Basel · Department Biomedizin

MD PhD; M.Chir

About

73
Publications
6,032
Reads
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2,944
Citations
Additional affiliations
August 2015 - present
Southeast University (China)
Position
  • Lecturer
October 2010 - July 2015
Universitätsspital Basel
Position
  • Researcher
October 2010 - January 2015
University of Basel
Position
  • MD/PhD student

Publications

Publications (73)
Article
Full-text available
Lower respiratory tract infections caused over 4 million deaths per year worldwide, especially in low-income countries. Viral respiratory infections often occur as rapidly spreading seasonal endemic or epidemic, and sometimes due to new respiratory viruses including corona viruses. The first level of host defense against viral infection is based on...
Article
Full-text available
Airway wall remodeling is a pathology occurring in chronic inflammatory lung diseases including asthma, chronic obstructive pulmonary disease, and fibrosis. In 2017, the American Thoracic Society released a research statement highlighting the gaps in knowledge and understanding of airway wall remodeling. The four major challenges addressed in this...
Article
Full-text available
In clinical studies, OM-85 Broncho-Vaxom®, a bacterial lysate, reduced viral respiratory tract infection. Infection of epithelial cells by SARS-CoV-2 depends on the interaction of its spike-protein (S-protein) with host cell membrane proteins. In this study, we investigated the effect of OM-85 on the expression of S-protein binding proteins by huma...
Article
Full-text available
Idiopathic pulmonary fibrosis (IPF) is a progressive terminal lung disease, and therapies aim to block fibrosis. Fibroblast proliferation is controlled by C/EBP-β, microRNA cluster 17-92 (miR17-92), and Erk1/2 mitogen-activated protein kinase. This study assessed the role of miR17-92 in IPF-fibroblast proliferation and its modification by treprosti...
Article
Airway epithelial mitochondrial oxidative stress and damage is an important pathology in chronic obstructive pulmonary disease (COPD). Mitophagy involves MAPK15-ULK1 signaling, the role of which is unknown in COPD. This study investigated MAPK15-ULK1 signaling in airway epithelial cells of COPD patients and its activation by cigarette smoke extract...
Article
Full-text available
Background: Bronchial thermoplasty regulates structural abnormalities involved in airway narrowing in asthma. In the present study we aimed to investigate the effect of bronchial thermoplasty on histopathological bronchial structures in distinct asthma endotypes/phenotypes. Methods: Endobronchial biopsies (n = 450) were collected from 30 patient...
Article
Background Increased airway smooth muscle mass is a key pathology in asthma. Bronchial thermoplasty is a treatment for severe asthma based on selective heating of the airways that aims to reduce the mass of airway smooth muscle cells (ASMC), and thereby bronchoconstriction. However, short heat exposure is insufficient to explain the long-lasting ef...
Article
Full-text available
Oral bacterial lysates (OBLs) can reduce the frequency and severity of recurrent respiratory tract infections in children from viral and bacterial origins. OBL-induced early innate immune reaction was already shown, but the specific features of different OBLs have never been studied and compared. A study was conducted to assess in vitro the protect...
Article
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Asthma is characterised by chronic inflammation and tissue remodelling of the airways. Remodelling is resistant to pharmaceutical therapies. This study investigated the effect of zinc salicylate-methylsulfonylmethane (Zn-Sal-MSM) compared to zinc salicylate (Zn-Sal), or sodium salicylate (Na-Sal) or zinc chloride (ZnCl2) on remodelling parameters o...
Article
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The aim of this retrospective study is to determine the predictive factors of postoperative dyspnea in infants with Pierre Robin sequence (PRS). Forty children with PRS, who underwent general anesthesia, were retrospectively analyzed. The patient’s physiological status and anesthesiology data were collected accordingly, demographic characteristics...
Preprint
Full-text available
Rationale In idiopathic pulmonary fibrosis (IPF) atypical epithelial cells are present in the alveolar compartment. Their origin and contribution to IPF pathogenesis is unknown. We recently cultured a distinct population of cells, which readily grew from fibrotic lung tissue, but only rarely from non-fibrotic tissue. Here we aimed to characterize t...
Article
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Asthma increases worldwide without any definite reason and patient numbers double every 10 years. Drugs used for asthma therapy relax the muscles and reduce inflammation, but none of them inhibited airway wall remodeling in clinical studies. Airway wall remodeling can either be induced through pro-inflammatory cytokines released by immune cells, or...
Article
Bronchial thermoplasty (BT) is to date the only therapy, which lastingly reduces airway wall remodelling. However, the mechanism of action of BT is not well understood. This study aimed to characterise the changes of remodelling regulating signalling pathways by BT in asthma. Bronchial alveolar lavage fluid (BALF) was obtained from eight patients w...
Article
Purpose: Adrenomedullin (AM) is a pluripotent peptide hormone with contradictory effects in human health and disease. In chronic inflammatory lung diseases, such as asthma and COPD, AM has been shown to inhibit inflammation and cell proliferation. In the present study, we aimed to investigate the effect of AM on pro-angiogenic and pro-inflammatory...
Article
Airway smooth muscle cell (ASMC) hyperplasia causes airway wall remodelling, which is resisting to therapy. Long acting β2-agonists (LABA) relax airway muscles, but their effect on remodelling is unclear. This study compared the anti-proliferative effect of LABA in human primary ASMC, in situations where LABA were applied before, together, or after...
Article
Full-text available
The patho-mechanism leading to airway wall remodeling in allergic asthma is not well understood and remodeling is resistant to therapies. This study assessed the effect of immunoglobulin E (IgE) in the absence of allergens on human primary airway smooth muscle cell (ASMC) remodeling in vitro. ASMCs were obtained from five allergic asthma patients a...
Article
Full-text available
Bronchial epithelial cells are the first target cell for rhinovirus infection. The course of viral infections in patients with acute bronchitis, asthma and COPD can be improved by oral application of Pelargonium sidoides radix extract; however, the mechanism is not well understood. This study investigated the in vitro effect of Pelargonium sidoides...
Data
Tables A and B: Optical density values derived from Fig C by image analysis (imageJ). Data is shown for the same patients shown in S2 File. Mean, S.D. and S.E.M. as well as Student’s t-test were performed by Excel program. Fig C: Representative Western-blots of ICOS and ICOSL. Protein bands used to calculate optical density values presented in Tabl...
Data
Figs (A) Phase-contrast microscopy of primary human bronchial epithelial cells. Epithelial cells show knobble-stone phenotype (day 0). EPs 7630 alone, second row, did not alter the phenotype over 2 days; while RV16 infection significantly reduced cell numbers (3rd row, 1st photograph). When pre-incubated for 1 day with EPs 7630 cell survival was in...
Data
Table A: Optical density values derived from Fig B by image analysis (imageJ). Data is shown for the same patients shown in S2 File. Mean, S.D. and S.E.M. as well as Student’s t-test were performed by Excel program. Fig B: Representative Western-blots of C1qR. Protein bands used to calculate optical density values presented in Table A are marked by...
Data
Table A: Number of surviving human bronchial epithelial cells after RV16 infection for 6 healthy controls (H01-H06), 6 asthma patients (A01-A06), and 6 COPD patients (CD01-CD06). Table B: Number of RV16 infected cells for the same patients presented in table A. Mean, S.D. and S.E.M. as well as Student’s t-test were performed by Excel program. (PDF)
Data
Table A: Optical density values derived from Fig B by image analysis (imageJ). Data is shown for the same patients shown in S2 File. Mean, S.D. and S.E.M. as well as Student’s t-test were performed by Excel program. Fig B: Representative Western-blots of β-defensin1. Protein bands used to calculate optical density values presented in Table A are ma...
Data
Table A: Optical density values for SOCS1 obtained by cell based ELISA in the same patients shown in S2 File. Mean, S.D. and S.E.M. as well as Student’s t-test were performed by Excel program. (PDF)
Article
Full-text available
Tissue remodeling of subepithelial mesenchymal cells is a major pathologic condition of chronic obstructive pulmonary disease and asthma. Fibroblasts contribute to fibrotic events and inflammation in both airway diseases. Recent mechanistic studies established a link between mitochondrial dysfunction or aberrant biogenesis leading to tissue remodel...
Article
Full-text available
Purpose To evaluate a comprehensive scoring system which combines clinical manifestations of Pierre Robin Sequence (PRS) including severity of breathing difficulties, body weight and preoperative Cormack-Lehane grade, for its correlation with perioperative PRS airway management decision. Design Forty PRS children were retrospectively recruited aft...
Article
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Previous work suggests that aging is associated with changes in risk taking but less is known about their underlying neural basis, such as the potential age differences in the neural processing of value and risk. The goal of the present study was to investigate adult age differences in functional neural responses in a naturalistic risk-taking task....
Article
Nucleotide-binding domains and leucine-rich repeat (NLR) pyrin domains containing 3 (NLRP3) inflammasome are highly involved in the pathogenesis of acute lung injury (ALI) wherein alveolar macrophages (AMs) play a crucial role. Isoflurane (ISO) has been shown to attenuate ALI. However, the inhibitory effects of ISO on NLRP3 activation in lipopolysa...
Article
Full-text available
Microfluidic electrical impedance spectroscopy (EIS) has been widely used for the identification of single cells based on the detection of cell size or morphology. However, the obtained impedance data are highly affected by the design of microfluidic setup and by the geometric parameter and orientation of samples. Here, we systematically investigat...
Article
Higher eukaryotic organisms cannot live without oxygen; yet, paradoxically, oxygen can be harmful to them. The oxygen molecule is chemically relatively inert because it has two unpaired electrons located in different pi * anti-bonding orbitals. These two electrons have parallel spins, meaning they rotate in the same direction about their own axes....
Article
Purpose: Oxidative stress plays an important role in the pathogenesis of glaucomatous optic neuropathy. One indirect measure of oxidative stress is the quantification of DNA breaks by comet assay. This study aims to quantify single-stranded DNA (ss-DNA) breaks in circulating leukocytes of glaucoma patients treated with Ginkgo biloba and Nifedipine...
Article
The purpose was to measure the retinal venous pressure (RVP) in both eyes of primary open-angle glaucoma (POAG) patients before and 3 weeks after treatment with low-dosed Nifedipine. This retrospective study included 20 POAG patients who were treated with Nifedipine (5 mg daily) and 20 untreated control POAG patients. In both the treated and untrea...
Article
Full-text available
Maintaining mitochondrial function is essential for neuronal survival and offers protection against neurodegeneration. Ubiquitin-mediated, proteasome-dependent protein degradation in the form of outer mitochondrial membrane associated degradation (OMMAD) was shown to play roles in maintenance of mitochondria on the level of proteostasis, but also m...
Article
Full-text available
Background The purpose of the study was to measure the retinal venous pressure (RVP) in the eyes of primary open-angle glaucoma (POAG) patients and healthy subjects with and without a Flammer-Syndrome (FS). Methods RVP was measured in the following four groups of patients and age- and sex-matched healthy controls: (a) 15 patients with a POAG and a...
Article
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Meningothelial cells (MECs) are the cellular components of the meninges. As such, they provide important barrier function for the central nervous system (CNS) building the interface between neuronal tissue and the cerebrospinal fluid (CSF), and are also part of the immune response of the CNS. Human, immortalized MECs were analyzed by flow cytometry...
Article
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Neuronal cell death is accompanied by mitochondrial dysfunction with mitochondrial maintenance critical to neuronal survival. The mitochondrial ubiquitin ligase MARCH5 has dual roles in the upkeep of mitochondrial function. MARCH5 is involved in targeted degradation of proteins harmful to mitochondria and impacts mitochondrial morphology upstream o...
Article
Background information: Meningothelial cells (MECs) are the cellular components of the meninges protecting the brain and as such provide important barrier function for the central nervous system building the interface between neuronal tissue and the cerebrospinal fluid (CSF). MECs were previously shown to be involved in the clearance of waste prod...
Article
Full-text available
To study the impact of the mitochondrial ubiquitin ligase MARCH5 on mitochondrial morphology and induction of apoptosis using an in vitro model of neuronal precursor cells exposed to glaucoma-relevant stress conditions. RGC5 cells transfected with expression constructs for MARCH5, MARCH5(H43W), Dpr1(K38A) or vector control were exposed to either el...
Article
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The peroxisome proliferator-activated receptor γ coactivator 1 (PGC-1) proteins are key regulators of cellular bioenergetics and are accordingly expressed in tissues with a high energetic demand. For example, PGC-1α and PGC-1β control organ function of brown adipose tissue, heart, brain, liver and skeletal muscle. Surprisingly, despite their promin...
Data
Gene expression analysis of WT and PGC-1α KO mice in light and dark. (a) Microarray analysis of light and dark exposed PGC-1α KO and C57BL/6j WT control mice; Gene expression changes in the four comparisons: 18sRNA was used as HKG. +SEM for n = 3; * = p<0.05; ** = p<0.01; *** = p<0.001. Statistical significance was calculated using two tailed Stude...
Data
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List of primers for semi-quantitative real time PCR analysis. (PDF)
Data
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Microarray analysis of light and dark exposed PGC-1α KO and C57BL/6j WT control mice: top genes and pathways. (a) Top 10 genes most highly up-/downregulated in their mRNA expression of PGC-1α KO and C57BL/6j WT mice, dark vs light exposed. (b) Top 10 pathways up-/downregulated in their mRNA expression of PGC-1α KO and C57BL/6j WT mice, dark vs ligh...
Data
Full-text available
Microarray analysis of light and dark exposed PGC-1α KO and C57BL/6j WT control mice. Significant changes in genes involved in apoptosis, DNA repair, inflammation and phototransduction. green = upregulated; red = downregulated (a) pro and anti-apoptotic, DNA repair genes (b) phototransduction and protein folding genes (c) ECM breakdown and inflamma...
Article
To explore the protective effect of the phosphodiesterase-5 inhibitor, sildenafil, on lung ischemia-reperfusion injury, 30 rats were randomly divided into 3 groups of 10: a sham-operated group A, a lung ischemia-reperfusion injury group B, and a sildenafil preconditioned group C. A 0.1% sildenafil solution was administrated orally 2 h before establ...