Leandro Godoy

Leandro Godoy
Universidade Federal do Rio Grande do Sul | UFRGS · Departamento de Zootecnia

PhD

About

53
Publications
15,787
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338
Citations
Introduction
Over the last years, I have dedicated my researches in the field of cryobiology applied on reproduction of aquatic organisms. My current researches aim to develop cryopreservation protocols gametes and tissues and understanding the cryopreservation effects on metabolism of reproductive cells. Biochemistry and physiology of gametes from Brazilian corals as well as biotechnology are applied by our group to support coral reefs conservation.
Additional affiliations
January 2016 - January 2017
Universidade Federal do Rio Grande do Sul
Position
  • Professor (Associate)
August 2013 - December 2015
Universidade Nilton Lins
Position
  • Professor of Fish Reproduction
January 2012 - present
Universidade Federal do Rio Grande do Sul
Position
  • Development of cryopreservation protocols for gametes and embryos of migratory fish
Description
  • International project funded by CAPES Foundation (Ministry of Education of Brazil) by the Program Science without Borders. Partnership in research with Bournemouth University (UK)
Education
July 2011 - August 2012
University of Bedfordshire
Field of study
  • Cryobiology
March 2009 - August 2012
Universidade Federal do Rio Grande do Sul
Field of study
  • Cryopreservation of fish oocytes
August 2006 - July 2008

Publications

Publications (53)
Article
A preservation protocol has not been established for Colossoma macropomum oocytes, and its development may improve the production and breeding programs of this South American fish species. Thus, this study aimed to determine the effect of different methods and protocols for the preservation of C. macropomum oocytes. Seven experiments were conducted...
Article
Given the current threatening scenario faced by coral reefs, reproductive biotechnologies can be a helping hand in the fight against extinction. Therefore, safe methods to assess viability of coral gametes are essential. In this study, we aimed to develop a reliable method to assess oocyte viability for scleractinian corals using Mussismilia hartti...
Article
O objetivo deste estudo foi desenvolver e caracterizar farinhas a partir de carcaças de tilápia, pacu e carpa para inclusão em produtos alimentícios, visando o enriquecimento nutricional. Carcaças das três espécies foram defumadas a quente e posteriormente submetidas a moagem, resultando nas farinhas. Foram realizadas análises dos rendimentos do pr...
Article
Fatty acids (FA) accumulated during follicular development are essential for oocyte growth and maturation, as well as for the embryo generated. Alterations in the FA profile can compromise the oocyte development viability after cryostorage. Thus, our aim of this study was to analyze the possible changes in the FA profile generated during vitrificat...
Article
Bleaching events are becoming more frequent and intense worldwide. Southwestern Atlantic corals are considered stress-tolerant species that suffer less during bleaching episodes. Therefore, we investigated if the reef-building corals Mussismilia hispida and M. harttii are capable of spawning while fully bleached. We compared spermatozoa concentrati...
Article
Reproduction in fish is influenced by several environmental factors. In many cases, failure in reproduction is common once fish are no longer in their natural habitat. Thus, the use of exogenous hormones has been crucial to control the reproductive cycle of many fish species under ex situ conditions. These hormonal stimulations became yet more impo...
Poster
Full-text available
In fish, epigenetic mechanisms in ovarian follicle remain poorly understood. The gametes are carrying not only genetic but epigenetic information that will be transferred to the progeny. Cryopreservation of ovarian follicles is a promising method to preserve genetic material in fish. However, very little is known on the most with regards to possibl...
Article
This study aimed to describe the morphology and sperm quality of free-living adult males of cururu stingray Potamotrygon wallacei , endemic from the Rio Negro basin, Brazilian Amazon. The sperm was collected in loco from the seminal vesicle region and fixed in buffered saline formaldehyde solution for further evaluation of morphometry, sperm plasma...
Article
Hypancistrus zebra is an ornamental fish endemic to the Xingu River (Brazilian Amazon) and is critically endangered by the construction of a hydroelectric plant in its habitat and illegal fishing. In an attempt to create a germplasm bank for conservation purposes, in the present study there was characterization of H. zebra sperm for the first time...
Article
Our study assessed the efficiency of a formulated new extender in maintaining viability and morphological integrity of Colossoma macropomum spermatozoa under chilling storage. Semen was diluted in the test extender and BTS™ (Beltsville Thawing Solution) and exposed to a short‐term storage at 4.6 ± 0.6°C for 96 hr. Both extenders were able to mainta...
Article
Full-text available
Nesse artigo, capa da edição N° 15 da Revista Aquaculture Brasil, é discutida a importância dos recifes de coral e como as mudanças climáticas estão afetando esse ecossistema. Detalho o histórico da criação do Projeto ReefBank, nossas atividades de pesquisa e extensão.
Article
Full-text available
Currently, there is no effective technique to evaluate the quality of oocytes in fish farming in a practical and affordable way. The cell membrane integrity test with the vital dye trypan blue (TB) could be an option. In this study, Colossoma macropomum and Brycon amazonicus oocytes were exposed to different TB concentrations seeking to verify a po...
Article
Full-text available
The objective of this work was to analyze the proximate and fatty acid composition of the dorsal and ventral muscles of wild pirarucu (Arapaima gigas) captured from a Brazilian Amazonian lake. Dorsal and ventral muscles were dissected out, freeze-dried, vacuum-packed, and had the proximate and fatty acid composition analyzed. Ash, total proteins, a...
Article
Full-text available
DNA methylation patterns are inherited from parents and are imperative for proper embryonic development; however, alterations in these patterns can compromise fertilization and development into a fully functioning adult animal because DNA methylation is part of a complex program of gene transcription. In this study, we investigated the impact of cr...
Chapter
Full-text available
Studies on semen cryopreservation in Brazilian freshwater fish have been growing in number of publications and investigated species. Despite this apparent increase in research, standardization of cryoprotocols is still missing, making it clear that the grounds on the quality of cryopreserved semen has not yet reached a level that guarantee satisfac...
Cover Page
Full-text available
Histórico da origem dos exemplares de H. zebra que formaram nosso plantel de reprodutores e contribuíram para a execução de diversas pesquisas.
Article
Full-text available
This study aimed to evaluate muscle organization in tambaqui in order to describe the muscle growth process. We analyzed the morphometric pattern of fibers from white muscle of young-adults (300 days) by smaller diameter. The organization of white muscle exhibited a typical morphological pattern found in other fish species. Heavier animals showed h...
Article
Full-text available
Background: Over the last ten years, Brazilian fish farming has become more focused, resulting in the development of genetic improvement programmes (GIP) for two South American species Colossoma macropomum (tambaqui) and Pseudoplatystoma reticulatum (cachara). Objective: To describe the action plan used for setting up the GIP and to detail the g...
Article
Full-text available
Cryopreservation of ovarian tissue has been studied for female germline preservation of farm animals and endangered mammalian species. However, there are relatively few reports on cryopreservation of fish ovarian tissue and especially using vitrification approach. Previous studies of our group has shown that the use of a metal container for the cry...
Research
Full-text available
El alimento y el proceso de alimentación representan el costo operativo más fuerte de la actividad acuícola en peces y crustáceos, además de ser la principal fuente de contaminación de sus efluentes. El aprovechamiento del alimento natural que se da en forma espontánea, o que se induce en los sistemas de cultivo, es una de las alternativas más viab...
Chapter
Full-text available
The importance of animal genetic resources for wildlife maintenance as well as farming production has become more and more evident in recent years. Fish stocks are globally threatened mainly due to overfishing and environmental pollution. Cryopreservation of aquatic germplasm brings the possibility of preserving the genome of endangered species, in...
Article
Attempts to cryopreserve fish embryos have been conducted over the past three decades, nevertheless successful cryopreservation protocol for long-term storage still remains elusive. Fish oocytes offer some advantages when compared to embryos, which may help in improving the chances of cryopreservation. In the present study, a series of cryo-solutio...
Article
This study evaluated the use of fish processing residues in the preparation of flavored meal from Nile tilapia (Oreochromis niloticus) carcass. Carcasses were washed, labeled, weighed, spice-brined and smoked. After smoking, the carcasses were weighed and ground to a meal. The samples were vacuum packed, labeled and frozen (−18C) until analysis. Th...
Article
Full-text available
Overfishing and environmental factors such as pollution have contributed to the significant decline of fish populations worldwide. Successful cryopreservation of fish gametes and embryos would have important applications in conservation and aquaculture. Although cryopreservation of fish semen has already been achieved successfully, cryopreservation...
Article
The conservation of semen may have its costs reduced when used within a certain period of time through cooling techniques. These techniques are alternatives to reproductive management in fish farms, optimizing fertilization of oocytes and ensuring satisfactory reproduction. The quality of the extenders is essential, since it guarantees the maintena...
Article
Full-text available
Summary Although the sperm cryopreservation of freshwater and marine teleosts has been feasible for years, the cryopreservation of some fish embryos still remains elusive. Thus, the objective of this experiment was to analyze the embryo morphology after freezing and thawing 40 embryos of Piaractus mesopotamicus immersed into methanol and ethylene g...
Article
Full-text available
Bio-floc shrimp culture systems have been investigated in an attempt to optimize water use and prevent the discharge of effluent into the environment. The importance of microalgae in maintaining water quality and nutrition of the shrimp is well known in conventional systems; however, its maintenance amid bio-flocs and its role in the shrimp perform...
Article
Full-text available
Cryopreservation of fish gametes has been studied extensively in the last few decades, but the successful cryopreservation of fish embryos remains elusive. However, recent studies using short-term chilling techniques have shown that it is possible to store embryos at low temperatures with no significant loss in viability. Information on cryopreserv...
Article
Full-text available
SummaryCryopreservation of germplasm provides a promising method to preserve fish genetic material, which is of great importance in preservation of species diversity, aquaculture, and management of fish models used in biomedical research. In the present study, cryopreservation of Rhinelepis aspera embryos, a Brazilian endangered species, was studie...
Article
Full-text available
This study evaluated the effect of smoking and the presence of aromatic herbs on the quality of freshwater prawns muscle (Macrobrachium rosenbergii), through the analysis of sensory characteristics and proximate composition. Fifty-five freshwater thermally stunned prawns, with average weight of 33.9 ± 13.49g, were used. After cephalothoraxes and ca...
Article
Full-text available
This work evaluated the acceptance of soups and broths prepared with aromatized meals made from smoked fish carcasses. The species of fish used for smoking were Nile Tilapia, carp, and pacu. The carcasses were washed, labeled, weighted, immersed in a solution of brine with aromatic herbs, and smoked. The smoked product was crushed to obtain the mea...
Article
Full-text available
Realizaram-se testes físico-mecânicos e físico-químicos em couro de tilápia vermelha (Oreochromis spp.) a fim de testar a sua resistência. As amostras foram distribuídas em delineamento inteiramente ao acaso com dois tratamentos: no T1, procedeu-se à retirada do corpo-de-prova no sentido longitudinal e, no T2, à retirada do corpo-de-prova no sentid...
Article
Full-text available
Physical-mechanical and physical-chemical tests were carried out on red tilapia leather. They were distributed in a completely randomized design with two treatments: T1 = longitudinal body of proof; T2 = transversal body of proof. It was used the EMIC dynamometer for the tests of resistance to traction and elongation and the progressive tearing, wi...
Article
Full-text available
O presente trabalho objetivou avaliar o desempenho produtivo de três linhagens de tilápia-do-Nilo Oreochromis niloticus, alimentadas com dois níveis de proteína bruta (25 e 30%). As linhagens estudadas foram Bouaké (BOU), Chitralada (CHI) e GIFT. Os peixes forammarcados individualmente e alojados em dois viveiros de terra de 140 m2 cada, num deline...
Article
Full-text available
Este trabalho avaliou o efeito do alecrim na qualidade da carne de rã (Rana catesbeiana) defumada, por meio da análise das características sensoriais, da composição centesimal e do rendimento. Após o abate e a evisceração, as carcaças foram imersas em solução de salmoura (20%) numa proporção de 2:1 (volume da salmoura/peso). Posteriormente, elas fo...
Article
Full-text available
This work studied the effect of rosemary on the quality of smoked frog meat (Rana catesbeiana) through the analysis of sensory characteristics, centesimal composition, and processing performance. The carcasses were immersed in a solution of brine (20%), in the proportion of 2:1 (volume of brine/weight), soaked in olive oil and smoked at a temperatu...
Article
Full-text available
No presente experimento objetivou-se avaliar a resistência das peles de peixes submetidas ao curtimento com taninos vegetal e sintético. As peles foram distribuídas em um delineamento inteiramente casualizado, com seis tratamentos aplicados na etapa de curtimento (T1 = 10% tanino vegetal; T2=10% tanino sintético; T3 = 5% tanino vegetal + 5% sintéti...
Article
Full-text available
No presente experimento objetivou-se avaliar a resistência das peles de peixes submetidas ao curtimento com taninos vegetal e sintético. As peles foram distribuídas em um delineamento inteiramente casualizado, com seis tratamentos aplicados na etapa decurtimento (T1 = 10% tanino vegetal; T2=10% tanino sintético; T3 = 5% tanino vegetal + 5% sintétic...
Article
Full-text available
RESUMO -Este estudo foi realizado com os objetivos de analisar a histologia da pele e avaliar a influência da técnica de curtimento e da posição da retirada do corpo-de-prova sobre a qualidade da pele de carpa prateada (Hypophtalmichthys molitrix). Vinte e três peixes foram insensibilizados para retirada das peles para curtimento. Amostras referent...
Article
Full-text available
The objectives of this trial were to analyze the skin histology and to evaluate the influence of tanning technique and the direction removal of samples on skin quality of silver-plated carp (Hypophtalmichthys molitrix). Twenty-three fishes were sacrificed and the skin was removed for tanning. Samples of three fishes were settled in formol 10% and,...

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Projects (2)
Project
In fish, epigenetic regulation mechanisms in ovarian follicle throughout oogenesis and alterations in this regulation remain poorly understood. Data from mammal studies have indicated that epigenetic profile has a dynamic change during oogenesis, fertilization and early embryo development, and it is fundamental on chromatin packaging and gene transcription regulation. Epigenetic regulation is essential for ovarian follicle until the final maturation and maternally-inherited mRNAs accumulation, which have a direct impact on the progeny development. Recently, studies in mammals involving epigenetic regulation in cryopreserved gametes had identified changes in transcription of important genes and heat shock proteins caused by this process. Cryopreservation of ovarian follicles is a promising method to preserve genetic material in fish, contributing to the reproductive management and conservation of endangered species. For aquaculture species that reproduce only once a year, cryopreservation would allow the production of young forms throughout the year, improving commercial production. However, very little is known about epigenetic profile and the dynamic regulation in ovarian follicle, as well as the possible impacts of alterations generated by exposure to cryoprotectants agents and cryogenic temperature. The present study therefore aims to characterize the epigenetic profile and alterations generated by the cryopreservation process of the ovarian follicles on oogenesis in fishes.
Project
Researches like this one, focusing on in vitro reproduction and cryopreservation of gametes from Hypancistrus zebra can generate knowledge and subsidies to allow the conservation of this Amazonian endangered ornamental fish.