Laurent Toffin

Laurent Toffin
Institut Français de Recherche pour l'Exploitation de la Mer | Ifremer · Department of Biological Resources and Environment

PhD

About

45
Publications
6,764
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1,313
Citations
Citations since 2017
12 Research Items
629 Citations
2017201820192020202120222023020406080100
2017201820192020202120222023020406080100
2017201820192020202120222023020406080100
2017201820192020202120222023020406080100
Additional affiliations
January 2003 - present
Japan Agency for Marine-Earth Science Technology
January 2000 - present
Université de Bretagne Occidentale
Description
  • phD

Publications

Publications (45)
Article
A novel anaerobic methylotrophic halophilic methanogen strain SLHTYROT was isolated from a deep hypersaline anoxic basin called “Tyro” located in the Eastern Mediterranean Sea. Cells of SLHTYROT were motile cocci. The strain SLHTYROT grew between 12 and 37 °C (optimum 30 °C), at pH between 6.5 and 8.2 (optimum pH 7.5) and salinity from 45 to 240 g...
Chapter
Me.tha.no.coc.co'i.des. Gr. adj. suff. ‐oides similar to; N.L. neut. n. Methanococcoides organism similar to Methanococcus. Euryarchaeota / Methanomicrobia / Methanosarcinales / Methanosarcinaceae / Methanococcoides The genus Methanococcoides comprises four species, Methanococcoides methylutens, Methanococcoides burtonii, Methanococcoides alaskense...
Article
The depletion of traditional oil fields is driving the oil & gas industry to explore new exploitation sites previously considered as unprofitable. Deep-sea oil fields represent one of these new areas of exploitation. Well drilling during exploration and production operations generate large quantities of drilling waste whose biological impact on the...
Chapter
Benthic chambers are good tools to measure organism metabolism and chemical exchanges rates at the sediment–water interface. Three size categories are recognized in deep‐sea ecology for benthic fauna: meio‐, macro‐ and megafauna. Low microbial diversity with distinctive microbial communities also suggests that gas hydrate deposits are also a highly...
Article
Full-text available
We report here the complete genome sequence (2.08 Mb) of Methanohalophilus portucalensis strain FDF-1 T , a halophilic methylotrophic methanogen isolated from the sediment of a saltern in Figeria da Foz, Portugal. The average nucleotide identity and DNA-DNA hybridization analyses show that Methanohalophilus mahii , M. halophilus , and M. portucalen...
Article
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In the Guaymas Basin, the presence at a few tens of kilometers of cold seeps and hydrothermal vents coupled with comparable sedimentary settings and depths offer a unique opportunity to assess and compare the microbial community composition of these deep-sea ecosystems. The microbial diversity in sediments from three cold seep and two hydrothermal...
Article
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Sitting at ~5 000 m water depth on the Congo-Angola margin and ~ 760 km offshore of the West African coast, the recent lobe complex of the Congo deep-sea fan receives large amounts of fluvial sediments (3-5% organic carbon). This organic-rich sedimentation area harbors habitats with chemosynthetic communities similar to those of cold seeps. In this...
Article
Geochemical profiles (SO4²⁻, H2S, CH4, δ¹³CH4) and phylogenetic diversity of Archaea and Bacteria from two oceanographic cruises dedicated to the lobes sediments of the Congo deep-sea fan are presented in this paper. In this area, organic-rich turbidites reach 5000 m and allow the establishment of patchy cold-seep-like habitats including microbial...
Article
Full-text available
The complete genome sequence of Methanohalophilus halophilus DSM 3094 T , a member of the Methanosarcinaceae family and the Methanosarcianales order , consists of 2,022,959 bp in one contig and contains 2,137 predicted genes. The genome is consistent with a halophilic methylotrophic anaerobic lifestyle, including the methylotrophic and CO 2-H 2 met...
Article
The presently active region of the Congo deep-sea fan (around 330 000 km2), called the terminal lobes or lobe complex, covers an area of 2500 km2 at 4700–5100 m water depth and 750–800 km offshore. It is a unique sedimentary area in the world ocean fed by a submarine canyon and a channel-levee system which presently deliver large amounts of organic...
Article
Full-text available
The Sonora Margin cold seeps present a seafloor mosaic pattern consisting of different faunal assemblages and microbial mats. To better understand if sedimentary microbial communities reflect this patchy distribution, all major habitats were investigated using four complementary approaches: 16S rRNA gene sequence 454 pyrosequencing, quantitative po...
Article
Full-text available
The depth of oxygen penetration into marine sediments differs considerably from one region to another. In areas with high rates of microbial respiration, O2 penetrates only millimetres to centimetres into the sediments, but active anaerobic microbial communities are present in sediments hundreds of metres or more below the sea floor. In areas with...
Article
Full-text available
In the Sonora Margin cold seep ecosystems (Gulf of California), sediments underlying microbial mats harbor high biogenic methane concentrations, fuelling various microbial communities such as abundant lineages of Anaerobic Methanotrophs (ANME). However biodiversity, distribution and metabolism of the microorganisms producing this methane remain poo...
Article
Full-text available
Subsurface sediments of the Sonora Margin (Guaymas Basin), located in proximity of active cold seep sites were explored. The taxonomic and functional diversity of bacterial and archaeal communities were investigated from 1 to 10 meters below the seafloor. Microbial community structure and abundance and distribution of dominant populations were asse...
Article
Full-text available
vulcani sp. nov., a marine methylotrophic methanogen that uses betaine, choline and N,N-dimethylethanolamine for methanogenesis, isolated from a mud volcano, and emended description of the genus Methanococcoides A novel, strictly anaerobic, methylotrophic marine methanogen, strain SLH33 T , was isolated from deep sediment samples covered by an oran...
Article
Full-text available
A novel strictly anaerobic methylotrophic marine methanogen, strain SLHL33T, was isolated from deep sediment samples covered by an orange microbial mat collected from the Napoli Mud Volcano. Strain SLH33T stained Gram negative, was a non-motile irregular coccus and occurred singly. Cells utilized trimethylamine, dimethylamine, monomethylamine, meth...
Article
Full-text available
The Sonora Margin cold seeps present on the seafloor a patchiness pattern of white microbial mats surrounded by polychaete and gastropod beds. These surface assemblages are fuelled by abundant organic inputs sedimenting from the water column and upward-flowing seep fluids. Elevated microbial density was observed in the underlying sediments. A previ...
Article
Full-text available
Cold seeps, located along the Sonora Margin transform fault in the Guaymas Basin, were extensively explored during the 'BIG' cruise in June 2010. They present a seafloor mosaic pattern consisting of different faunal assemblages and microbial mats. To investigate this mostly unknown cold and hydrocarbon-rich environment, geochemical and microbiologi...
Data
##Assembly-Data-START## Assembly Method :: ARB v. 5.3 Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END##
Article
Marine mud volcanoes are geological structures emitting large amounts of methane from their active centres. The Amsterdam mud volcano (AMV), located in the Anaximander Mountains south of Turkey, is characterized by intense active methane seepage produced in part by methanogens. To date, information about the diversity or the metabolic pathways used...
Data
Closest relatives of representative clones from RNA-derived archaeal 16S rRNA gene libraries from depths 0 to 6 cm (Sto-0_6-rt) and 6 to 8 cm
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Full-text available
Pockmarks are seabed geological structures sustaining methane seepage in cold seeps. Based on RNA-derived sequences the active fraction of the archaeal community was analysed in sediments associated with the G11 pockmark, in the Nyegga region of the Norwegian Sea. The anaerobic methanotrophic Archaea (ANME) and sulfate-reducing bacteria (SRB) commu...
Article
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In September 2008, an expedition of the RV Urania was devoted to exploration of the genomic richness of deep hypersaline anoxic lakes (DHALs) located in the Western part of the Mediterranean Ridge. Approximately 40 nautical miles SE from Urania Lake, the presence of anoxic hypersaline lake, which we named Thetis, was confirmed by swath bathymetry p...
Article
Submarine mud volcanoes are a significant source of methane to the atmosphere. The Napoli mud volcano, situated in the brine-impacted Olimpi Area of the Eastern Mediterranean Sea, emits mainly biogenic methane particularly at the centre of the mud volcano. Temperature gradients support the suggestion that Napoli is a cold mud volcano with moderate...
Article
Full-text available
Microbial mats in marine cold seeps are known to be associated with ascending sulfide- and methane-rich fluids. Hence, they could be visible indicators of anaerobic oxidation of methane (AOM) and methane cycling processes in underlying sediments. The Napoli mud volcano is situated in the Olimpi Area that lies on saline deposits; from there, brine f...
Article
Full-text available
Siboglinid tubeworms in cold seep sediments can locally modify the geochemical gradients of electron acceptors and donors, hence creating potential microhabitats for prokaryotic populations. The archaeal communities associated with sediments populated by Oligobrachia haakonmosbiensis and Sclerolinum contortum Siboglinid tubeworms in the Storegga Sl...
Article
Identifying the function of uncultured microbes in their environments today remains one of the main challenges for microbial ecologists. In this article, we describe a new method allowing simultaneous analysis of microbial identity and function. This method is based on the visualization of oligonucleotide probe-conferred hybridization signal in sin...
Data
SIMSISH analysis of artificial mixture of 15N-labelled E. coli and Pyrococcus abyssi at natural isotopic composition. The I6-Eub338-Cy3 probe was applied directly to the mixture. All images are 10 μm × 10 μm. Acquisition time was 25 ms pixel−1. (A) 12C14N– secondary ion image, (B) 32S– secondary ion image, (C) 127I– secondary ion image, (D) superim...
Article
The finding of microbial life in the Earth’s deep subsurface raises the question as to how deep microbial ecosystems are able to survive under these extreme environmental conditions. To investigate the effect of increasing ambient pressure, a new quantitative approach was developed to monitor adaptive changes in the cell membrane phospholipid compo...
Article
Full-text available
A piezotolerant, mesophilic, marine lactic acid bacterium (strain LT20T) was isolated from a deep sub-seafloor sediment core collected at Nankai Trough, off the coast of Japan. Cells were Gram-positive, rod-shaped, non-sporulating and non-motile. The NaCl concentration range for growth was 0-120 g l(-1), with the optimum at 10-20 g l(-1). The tempe...
Article
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A novel piezotolerant, mesophilic, facultatively anaerobic, organotrophic, polarly flagellated bacterium (strain LT13aT) was isolated from a deep sediment layer in the Nankai Trough (Leg 190, Ocean Drilling Program) off the coast of Japan. This organism used a wide range of organic substrates as sole carbon and energy sources: pyruvate, glutamate,...
Article
Culturable bacteria were detected in deep-sea sediment samples collected from the Nankai Trough site 1173 (Ocean Drilling Program, ODP, Leg 190) at 4.15 m below the seafloor with 4791 m of overlying water. In this deep ocean near surface sediment, mainly fermentative heterotrophs, autotrophic acetogens and sulfate-reducing bacteria were enriched by...
Article
Culturable bacteria were detected in deep-sea sediment samples collected from the Nankai Trough site 1173 (Ocean Drilling Program, ODP, Leg 190) at 4.15 m below the seafloor with 4791 m of overlying water. In this deep ocean near surface sediment, mainly fermentative heterotrophs, autotrophic acetogens and sulfate-reducing bacteria were enriched by...
Article
Full-text available
Our understanding of the genetics of species of the best-studied hyperthermophilic archaea, Pyrococcus spp., is presently limited by the lack of suitable genetic tools, such as a stable cloning vector and the ability to select individual transformants on plates. Here we describe the development of a reliable host-vector system for the hyperthermoph...

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