Laurent Metzinger

Laurent Metzinger
Université de Picardie Jules Verne | UPJV · UR UPJV 4666 HEMATIM

PhD, DPharm
Group leader - Non-coding RNA team. Translational research in the Nephrology and Cardiology fields.

About

90
Publications
16,889
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2,697
Citations
Citations since 2016
43 Research Items
1301 Citations
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Introduction
Laurent Metzinger has completed his PhD in Biological Sciences and Pharmaceutical studies in Strasbourg, France and was a postdoctoral fellow from the University of Oxford in a leading lab on Duchenne muscular Dystrophy (Pr. Kay Davies). He works on microRNA regulation in the HEMATIM team in Amiens, and focuses on anemia and related vascular disorders associated with Chronic Kidney Disease. He has authored some of the first papers showing a role for microRNAs in CKD.
Additional affiliations
January 2018 - present
Université de Picardie Jules Verne
Position
  • Professor
Description
  • Hematology and immunology team. Focus on microRNAs in CKD. MicroRNA group
September 2009 - December 2018
Université de Picardie Jules Verne
Position
  • Professor
Description
  • Group manager: microRNA work in vascular disease.
September 2009 - March 2015
Université de Picardie Jules Verne
Position
  • Professor in biochemistry and molecular biology
Description
  • focus on vascular calcifications related on CKD. editorial board member for RNA & Diseases and Journal of Nephrology and Urology.
Education
September 1991 - September 1994
University of Strasbourg
Field of study
  • PhD Duchenne muscular dystrophy

Publications

Publications (90)
Article
Multiple molecular disorders can affect mechanisms regulating proliferation and differentiation of growth plate chondrocytes. Mutations in the TRIM37 gene cause the Mulibrey nanism, a heritable growth disorder. Since chondrocytes are instrumental in long bone growth that is deficient in nanism, we hypothesized that TRIM37 defect could contribute to...
Article
Full-text available
Chronic kidney disease (CKD) is a global health problem characterized by progressive kidney failure due to uremic toxicity and the complications that arise from it. Anemia consecutive to CKD is one of its most common complications affecting nearly all patients with end-stage renal disease. Anemia is a potential cause of cardiovascular disease, fast...
Article
Full-text available
Recent progress in genomic research has highlighted the genome to be much more transcribed than expected. The formerly so-called junk DNA encodes a miscellaneous group of largely unknown RNA transcripts, which contain the long non-coding RNAs (lncRNAs) family. lncRNAs are instrumental in gene regulation. Moreover, understanding their biological rol...
Article
Full-text available
Chronic kidney disease (CKD) is a major cause of death worldwide and is associated with a high risk for cardiovascular and all-cause mortality. In CKD, endothelial dysfunction occurs and uremic toxins accumulate in the blood. miR-126 is a regulator of endothelial dysfunction and its blood level is decreased in CKD patients. In order to obtain a bet...
Article
We used a multi-omics approach to study the impact of over-expression and inhibition of the microRNA miR-223, a pleiotropic regulator of metabolic-related disease, in a monocyte-macrophage cell line. We analyzed the levels of proteins, mRNAs, and metabolites in order to identify candidate molecules involved in miR-223 regulation, to determine disea...
Article
Full-text available
Renal diseases include different pathologies, such as acute kidney injury (AKI), chronic kidney disease (CKD), end-stage renal disease (ESRD), diabetic nephropathy (DN), kidney cancer, polycystic kidney disease, etc [...]
Article
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Atherosclerosis, in the ultimate stage of cardiovascular diseases, causes an obstruction of vessels leading to ischemia and finally to necrosis. To restore vascularization and tissue regeneration, stimulation of angiogenesis is necessary. Chemokines and microRNAs (miR) were studied as pro‐angiogenic agents. We analysed the miR‐126/CXCL12 axis and c...
Article
Full-text available
Background and Aims The gene program is controlled at the post-transcriptional level by the action of small non-coding RNAs known as microRNAs (miRNAs), short, single-stranded molecules that control mRNA stability or translational repression via base pairing with regions in the 3' untranslated region of their target mRNAs. Recently, considerable pr...
Article
Full-text available
Background and Aims Chronic kidney disease (CKD) is a global health condition characterized by a progressive deterioration of renal function due to high serum levels of uremic toxins. Anemia is a major trouble in CKD patients that contributes to a faster deterioration of renal failure, leading to cardiovascular disease and increasing morbimortality...
Article
Full-text available
Renal diseases are consecutive to a deregulation of gene expression regulated by non-coding RNAs. These non-coding RNAs were discovered at the turn of the 21st century when it was established that post-transcriptional regulation was performed through small non-coding RNAs, known as microRNAs (miRNAs). Up to 3,000 miRNAs are expressed by human cells...
Article
Full-text available
Aortic valve stenosis remains the most frequent structural heart disease, especially in the elderly. During the last decade, we noticed an important consideration and a huge number of publications related to the medical and surgical treatment of this disease. However, the molecular aspect of this degenerative issue has also been more widely studied...
Article
Full-text available
Objective Restenosis is a frequent complication of angioplasty. It consists of a neointimal hyperplasia resulting from progression and migration of vascular smooth muscle cells (VSMC) into the vessel lumen. The microRNA miR-223 was recently shown to be involved in cardiovascular diseases including atherosclerosis, vascular calcification and arteria...
Article
Full-text available
Several microRNAs (miRNAs) have been linked to chronic kidney disease (CKD) mortality, cardiovascular (CV) complications and kidney disease progression. However, their association with clinical outcomes remains poorly evaluated. We used real-time qPCR to measure serum levels of miR-126 and miR-223 in a large cohort of 601 CKD patients (CKD stage G1...
Article
Full-text available
microRNAs (miRNAs) represent a recent breakthrough regarding gene expression regulation. They are instrumental players known to regulate post-transcriptional expression. miRNAs are short single stranded RNAs that base-pair with target mRNAs in specific regions mainly within their 3′ untranslated region. We know now that miRNAs are involved in kidne...
Article
Full-text available
TRIpartite motif (TRIM) proteins are part of the largest subfamilies of E3 ligases that mediate the transfer of ubiquitin to substrate target proteins. In this review, we focus on TRIM37 in the normal cell and in pathological conditions, with an emphasis on the MULIBREY (MUscle-LIver-BRain-EYe) genetic disorder caused by TRIM37 mutations. TRIM37 is...
Presentation
Full-text available
Special issue of JCDD
Article
Full-text available
Introduction La dysrégulation des microARN est impliquée dans la physiopathologie de nombreuses maladies, y compris les maladies rénales et cardiovasculaires. Cependant, leur association avec l’évolution clinique dans ces affections reste mal évaluée. Les objectifs de cette étude étaient de quantifier l’expression de deux microRNA circulants, miR-1...
Conference Paper
Full-text available
Shedding a new light: The implication of microRNAs in chronic kidney disease
Article
MicroRNAs (miRNAs) are short single-stranded RNAs that control gene expression through base pairing with regions within the 3 0-untranslated region of target mRNAs. These small non-coding RNAs are now increasingly known to be involved in kidney physiopathology. In this review we will describe how miRNAs were in recent years implicated in cellular a...
Article
Cardiovascular disease is the leading cause of morbidity and mortality in developed countries. Stroke is associated with a marked disability burden and has a major economic impact; this is especially true for carotid artery stroke. Major advances in primary and secondary prevention during the last few decades have helped to tackle this public healt...
Article
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Background and aim of the study: The study aim was to compare the tissular expression of microRNAs (miRs) in bicuspid and tricuspid valves, and to evaluate their use as potential novel biomarkers of aortic valve calcification in bicuspid valves. Methods: A prospective single-center observational study was conducted on stenotic bicuspid and tricu...
Article
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Epigenetic sciences study heritable changes in gene expression not related to changes in the genomic DNA sequence. The most important epigenetic mechanisms are DNA methylation, posttranslational histone modification, and gene regulation by noncoding RNAs, such as microRNAs (miRNAs) and long noncoding RNAs (lncRNAs). Cardiovascular diseases (CVD) ar...
Chapter
Full-text available
Background: Vascular calcification has been recognized to be a major risk factor in chronic kidney disease (CKD) and downstream cardiovascular complications. Micro-RNAs (miRNAs) are small noncoding RNAs comprising 20-25 nucleotides that regulate gene expression by inhibiting or degrading the target mRNA, and constitute potential new biomarkers and...
Article
microRNAs comprise a novel class of endogenous small non-coding RNAs that have been shown to be implicated in both vascular damage and bone pathophysiology. Chronic kidney disease-mineral bone disorder (CKD-MBD) is characterized by vessel and bone damage secondary to progressive loss of kidney function. In this review, we will describe how several...
Article
Full-text available
The discovery of small RNAs has shed new light on microRNA (mRNA) regulation and a range of biological processes. The recognition that miRNAs-221 and -222 are sensitive regulators in the endothelium may enable the identification of novel biomarkers and therapeutic targets. Given that endothelial dysfunction precedes the development of atheroscleros...
Article
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Background MicroRNAs (miRNAs) are innovative and informative blood-based biomarkers involved in numerous pathophysiological processes. In this study and based on our previous experimental data, we investigated miR-126, miR-143, miR-145, miR-155 and miR-223 as potential circulating biomarkers for the diagnosis and prognosis of patients with chronic...
Article
Full-text available
Vascular calcification (VC) is prevalent in patients suffering from chronic kidney disease (CKD). High phosphate levels promote VC by inducing abnormalities in mineral and bone metabolism. Previously, we demonstrated that magnesium (Mg 2+ ) prevents inorganic phosphate- (Pi-) induced VC in human aortic vascular smooth muscle cells (HAVSMC). As micr...
Article
Full-text available
Cardiovascular disease (CD) and peripheral vascular disease (PVD) are leading causes of mortality and morbidity in western countries and also responsible of a huge burden in terms of disability, functional decline, and healthcare costs. Biomarkers are measurable biological elements that reflect particular physiological or pathological states or pre...
Article
Full-text available
Chronic kidney disease-mineral and bone disorder (CKD-MBD) is a common complication of CKD, and uremic toxins have been shown to be instrumental in this process. We have previously shown that miR-223 is increased in smooth muscle cells subjected to the uremic toxin inorganic phosphate (Pi). In the present study we investigated the influence of this...
Article
Full-text available
Embolization of carotid stenotic plaques is the direct cause of stroke in nearly 20% of cases. Genetic mechanisms and especially the roles played by microRNAs in the regulation of plaque destabilization and rupture are mostly unknown. The aim of this pilot study was to compare the expression of seven microRNAs allegedly involved in plaque growth an...
Article
Full-text available
Morphology and changes in gene expression of vascular endothelium are mainly due to shear stress and inflammation. Cell phenotype modulation has been clearly demonstrated to be controlled by small noncoding micro-RNAs (miRNAs). This study focused on the effect of laminar shear stress (LSS) on human endothelial cells (HUVECs), with an emphasis on th...
Conference Paper
Full-text available
e347. doi: 10.14800/rd.347; © 2014 by Metzinger-Le Meuth V, et al. Development of disease is often due to deregulation of gene expression. The gene program is controlled at the post-transcriptional level by the action of small non-coding RNAs known as microRNAs (miRNAs), short, single-stranded molecules that control mRNA stability or translational...
Article
Full-text available
MicroRNAs (miRNAs) are small, noncoding RNAs of 18-22 nucleotides in length that regulate post-transcriptional expression by base-pairing with target mRNAs. It is now clearly established that miRNAs are involved in most of the cell's physiopathological processes (including carcinogenesis and metabolic disorders). This review focuses on miR-223, whi...
Article
Full-text available
Vascular calcification arises during chronic kidney disease (CKD), and increases the risk of cardiovascular mortality. In CKD, alterations of cerebral circulation were linked with an increase in ischemic strokes and behavioral troubles. Studying pathophysiological mechanisms of calcifications and detecting new biomarkers in the cerebral circulation...
Article
Full-text available
Chronic kidney disease (CKD) is associated with vascular calcifications and atherosclerosis. There is a need for novel predictors to allow earlier diagnosis of these disorders, predict disease progression, and improve assessment of treatment response. We focused on microRNAs since they are implicated in a variety of cellular functions in cardiovasc...
Article
Full-text available
Backgound An elevated serum inorganic phosphate (Pi) level is a major risk factor for kidney disease and downstream vascular complications. We focused on the effect of Pi levels on human aortic vascular smooth muscle cells (VSMCs), with an emphasis on the role of microRNAs (miRNAs). Methodology/Principal Findings Exposure of human primary VSMCs in...
Data
miR-223 is expressed in VSMCs, and its expression is increased by high Pi. (DOCX)
Data
High Pi treatment does not induce cell apoptosis in VSMC. (DOCX)
Data
Expression of miR-223 relative to miR-143 in control or Pi treated VSMCs. (DOCX)
Data
Effect of pre-miR-223 and anti-miR-223 on expression of miR-143 and miR-223 in VSMCs. (DOCX)
Data
Pi treatment reduced actin cytoskeleton in VSMCs. (DOCX)
Data
Microscopic images showing VSMC SMα-actin and cortactin staining after high Pi treatment. (DOCX)
Data
Effect of a combination of over-expression of miR-223 and high Pi on VSMC migration. (DOCX)
Chapter
Full-text available
In the last 10 years, microRNAs (miRNAs) have emerged as critical regulators of numerous physiological and pathological mechanisms [1-2], including cardiac and vascular smooth muscle cell (VSMC) plasticity [3-5]. These small molecules (approx. 20 to 25 nucleotides) comprise a novel and abundant class of endogenous interfering RNAs. More than 1 500...
Article
V ascular smooth muscle cells (VSMCs) are able to perform both contractile and synthetic functions, which are associated with changes in morphology, proliferation, and migration rates and are characterized by the specific expres-sion of different marker proteins. Under normal physiological conditions, VSMC rarely proliferate in adult tissues, but u...
Article
Full-text available
The pleiotropic post-transcriptional regulator Hfq is an RNA chaperone that facilitates pairing interactions between small regulatory RNAs (sRNAs) and their mRNA targets in several bacteria. However, this classical pattern, derived from the Escherichia coli model, is not applicable to the whole bacterial kingdom. In this article we discuss the facu...