Laurent Ladepeche

Laurent Ladepeche
ICFO Institute of Photonic Sciences | icfo · Super-resolution Light Microscopy & Nanoscopy Lab

PhD, Neuroscience

About

15
Publications
2,034
Reads
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1,166
Citations
Citations since 2016
7 Research Items
853 Citations
2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140
2016201720182019202020212022020406080100120140
Additional affiliations
January 2018 - present
ICFO Institute of Photonic Sciences
Position
  • PostDoc Position
February 2014 - December 2017
ICFO Institute of Photonic Sciences
Position
  • PostDoc Position
January 2013 - November 2013
University of Bordeaux
Position
  • PostDoc Position

Publications

Publications (15)
Article
Full-text available
Anti-N-methyl-D-aspartate receptor (NMDAR) encephalitis is a severe neuropsychiatric disorder mediated by autoantibodies against the GluN1 subunit of the NMDAR. Patients' antibodies cause cross-linking and internalization of NMDAR, but the synaptic events leading to depletion of NMDAR are poorly understood. Using super-resolution microscopy, we stu...
Data
D-serine and glycine dose-response curves. Summary of Hill coefficient (nH), half maximal concentration (EC50) and n-values for the glycine/D-serine dose-response curves obtained on GluN2A- and GluN2B-NMDARs recombinants. p-Values (unpaired, two-tailed Student t-test) assess the difference between the EC50 obtained with D-serine and glycine. DOI: h...
Data
Co-agonist and NMDAR-subunit developmental switch. Summary of the effects of BsGO, RgDAAO, Ro25-6981 and zinc normalized to baseline (mean ± s.e.m) in slices obtained from rats at indicated ages (see Figure 5—figure supplement 1). n values are indicated as well as p-values (paired Student t-test) assessing the significance of the effect achieved co...
Data
Capillary electrophoresis measurements. Summary of the measurements of glutamate, glycine and D-serine content in slices at different ages. Values indicate average content (in nmoles per mg of tissue) ± s.e.m. p-Values indicate the level of significance (student t-test) between values in <P10 slices compared to values in adults. DOI: http://dx.doi....
Article
The subunit composition of synaptic NMDA receptors (NMDAR), such as the relative content of GluN2A- and GluN2B-containing receptors, greatly influences the glutamate synaptic transmission. Receptor co-agonists, glycine and D-serine, have intriguingly emerged as potential regulators of the receptor trafficking in addition to their requirement for it...
Article
Full-text available
N-methyl-d-aspartate receptors (NMDARs) are ion channels whose synaptic versus extrasynaptic localization critically influences their functions. This distribution of NMDARs is highly dependent on their lateral diffusion at the cell membrane. Each obligatory subunit of NMDARs (GluN1 and GluN2) contains two extracellular clamshell-like domains with a...
Article
Identifying the underlying cellular mechanisms of episodic memory is an important challenge, since this memory, based on temporal and contextual associations among events, undergoes preferential degradation in aging and various neuropsychiatric disorders. Memory storage of temporal and contextual associations is known to rely on hippocampal N-methy...
Article
NMDA-type glutamate receptors (NMDAR) are central actors in the plasticity of excitatory synapses. During adaptive processes, the number and composition of synaptic NMDAR can be rapidly modified, as in neonatal hippocampal synapses where a switch from predominant GluN2B- to GluN2A-containing receptors is observed after the induction of long-term po...
Article
Full-text available
Significance Dopamine receptor signaling in the brain participates in memory encoding through the regulation of glutamatergic signaling. Here we provide evidence that single dopamine D1 receptors are highly dynamic at the surface of hippocampal neurons. In addition, these receptors, together with glutamatergic NMDA receptors, form surface clusters...
Article
Full-text available
Dopamine receptor potently modulates glutamate signalling, synaptic plasticity and neuronal network adaptations in various pathophysiological processes. Although key intracellular signalling cascades have been identified, the cellular mechanism by which dopamine and glutamate receptor-mediated signalling interplay at glutamate synapse remain poorly...
Article
Full-text available
During frog metamorphosis, the vestibular sensory system remains unchanged, while spinal motor networks undergo a massive restructuring associated with the transition from the larval to adult biomechanical system. We investigated in Xenopus laevis the impact of a pre- (tadpole stage) or post-metamorphosis (juvenile stage) unilateral labyrinthectomy...
Article
Full-text available
l-DOPA-induced dyskinesia (LID), a detrimental consequence of dopamine replacement therapy for Parkinson's disease, is associated with an alteration in dopamine D1 receptor (D1R) and glutamate receptor interactions. We hypothesized that the synaptic scaffolding protein PSD-95 plays a pivotal role in this process, as it interacts with D1R, regulates...
Article
N-methyl-d-aspartate receptors (NMDARs) are located in neuronal cell membranes at synaptic and extrasynaptic locations, where they are believed to mediate distinct physiological and pathological processes. Activation of NMDARs requires glutamate and a coagonist whose nature and impact on NMDAR physiology remain elusive. We report that synaptic and...

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Projects

Projects (2)
Project
Until recently, the diffraction limit was preventing the access to details about receptor nano-organization and dynamics due to the small size of synapses (250-500 nm wide). The newly developed super-resolution microscopy techniques can now overcome these challenges. We use Stochastic Optical Reconstruction Microscopy (STORM) to observe the effect of anti-NMDAR patient autoantibodies on the nanoscale organization and stoichiometry of the synaptic components.