Laurent Duca

Laurent Duca
Université de Reims Champagne-Ardenne | URCA · UMR CNRS 7369 MEDyC (Matrice Extracellulaire et Dynamique Cellulaire)

PhD

About

82
Publications
12,183
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Introduction
My research interests lie in the area of extracellular matrix/cell interactions, and especially in elastin/elastin peptides signaling through the Elastin Receptor Complex in the fields of vascular pathologies, remodelling and aging. Our team uses combined Biochemical / Physiological / Biophysical / Imaging approaches to characterize the elastin peptides involvement in diseases to propose innovative predictive and pharmacological strategies based on extracellular matrix biology
Additional affiliations
September 2018 - present
Université de Reims Champagne-Ardenne
Position
  • Professor (Full)
Description
  • Head of UMR CNRS 7369 MEDyC Team 2 "Matrix Aging and Vascular Remodelling"
September 2006 - August 2018
Université de Reims Champagne-Ardenne
Position
  • Professor (Associate)
Description
  • Head of UMR CNRS 7369 MEDyC Team 2 "Matrix Aging and Vascular Remodelling" since January 2017
September 2005 - August 2006
Paul Sabatier University - Toulouse III et Institut de Recherche Pierre Fabre
Position
  • PostDoc Position
Description
  • Extracellular Matrix and Wound Healing (Cutaneous and Corneal)

Publications

Publications (82)
Chapter
The vascular wall is a composite environment made of a myriad of different cell types and a complex extracellular matrix (ECM). The latter has a direct impact on vessel function and pathophysiological remodeling of ECM is associated with alterations of vessel structure and modifications of cell phenotype. In this context, elastin plays a fundamenta...
Article
Full-text available
Background Vascular aging is associated with remodeling of elastin, one of the main extracellular matrix component of the arterial wall, and production of elastin-derived peptides (EDP). These extracellular matrix degradation products have been shown to trigger biological activities through the elastin receptor complex (ERC) and data from the last...
Article
Full-text available
Abstract Numerous recent studies have shown that in the continuum of cardiovascular diseases, the measurement of arterial stiffness has powerful predictive value in cardiovascular risk and mortality and that this value is independent of other conventional risk factors, such as age, cholesterol levels, diabetes, smoking, or average blood pressure. V...
Article
Full-text available
Because of their long lifespan, matrix proteins of the vascular wall, such as elastin, are subjected to molecular aging characterized by non-enzymatic post-translational modifications, like carbamylation which results from the binding of cyanate (mainly derived from the dissociation of urea) to protein amino groups. While several studies have demon...
Article
Background Lotus maritimus L. (Fabaceae) is a perennial herb species with yellow flowers, growing in Europe, Asia, Middle East and Maghreb. Some flavones and flavonols have been identified in their leaves and flowers. Their leaf extract was used as a cellulolytic and antiaging cosmetic ingredient. Objective The aim of this work was to perform anti...
Article
Full-text available
Sialidases, also called neuraminidases, are involved in several human pathologies such as neurodegenerative disorders, cancers, as well as infectious and cardiovascular diseases. Several studies have shown that neuraminidases, such as neuraminidase 1 (NEU-1), may be promising pharmacological targets. Therefore, the discovery of new selective inhibi...
Article
Full-text available
Elastic fibers are extracellular components of higher vertebrates and confer elasticity and resilience to numerous tissues and organs such as large blood vessels, lungs and skin. Their formation and maturation take place in a complex multistage process called elastogenesis. It requires interactions between very different proteins but also other mol...
Article
Desialylation, governed by sialidases or neuraminidases, is strongly implicated in a wide range of human disorders, and accumulative data show that inhibition of neuraminidases, such as NEU1 sialidase, may be useful for managing atherosclerosis. Several studies have reported promising effects of oseltamivir phosphate, a widely used anti-influenza s...
Article
Full-text available
Extracellular matrix (ECM) is a dynamic 3-dimensional network of macromolecules that provides structural support for the cells and tissues. Accumulated knowledge clearly demonstrated over the last decade that ECM plays key regulatory roles since it orchestrates cell signaling, functions, properties and morphology. Extracellularly secreted as well a...
Preprint
Full-text available
Vascular stiffness is often the main cause of arterial hypertension and its complications including atherosclerosis, observed during obesity. However, the mechanisms leading to this rigidity or the preventing factors are misknown. We hypothesized that apelin, known for its beneficial effects on lipid, inflammatory, and vascular metabolism, could be...
Article
Arterial stiffness is a complex process affecting the aortic tree that significantly contributes to cardiovascular diseases (systolic hypertension, coronary artery disease, heart failure or stroke). This process involves a large extracellular matrix remodeling mainly associated with elastin content decrease and collagen content increase. Besides va...
Article
Full-text available
It is now admitted that in addition to acquired resistance, the tumor microenvironment contributes to the development of chemo-resistance and malignant progression. In a previous study, we showed that Dox induced apoptosis in FTC-133 cells by trigging JNK pathway. This process was accompanied by a decrease of thrombospondin-1 (TSP-1) expression. Mo...
Article
Full-text available
Sialidases, or neuraminidases, are involved in several human disorders such as neurodegenerative, infectious and cardiovascular diseases, and cancers. Accumulative data have shown that inhibition of neuraminidases, such as NEU1 sialidase, may be a promising pharmacological target, and selective inhibitors of NEU1 are therefore needed to better unde...
Article
Elastin, the major protein of the extracellular matrix, is specially found in cardiovascular tissues and contributing to 30–50% of the dry weight of blood vessels. Elastin regulates cell signalling pathways involved in morphogenesis, injury response and inflammation. The function of elastin is frequently compromised in damaged or aged elastic tissu...
Article
Full-text available
Cellular functions are regulated by extracellular signals such as hormones, neurotransmitters, matrix ligands, and other chemical or physical stimuli. Ligand binding on its transmembrane receptor induced cell signaling and the recruitment of several interacting partners to the plasma membrane. Nowadays, it is well-established that the transmembrane...
Article
Full-text available
This study presents the bioguided chemical investigation of the 80% aqueous methanol extract of Geum urbanum aerial parts. Liquid–liquid partitioning of this extract in solvents of increasing polarity combined with biological screening showed that the ethyl acetate (EtOAc) soluble fraction was the most active part of the extract. This fraction was...
Article
This study presents the bio-guided chemical investigation of a 80% methanol extract of Hippocrepis emerus flowers, a perennial non-climbing shrub. Liquid-liquid partitioning in solvents of increasing polarity combined to biological screening enabled to determine the EtOAc and n-BuOH soluble fractions as the most active parts of the extract. These f...
Article
Extracellular matrix (ECM) has for a long time being considered as a simple architectural support for cells. It is now clear that ECM presents a fundamental influence on cells driving their phenotype and fate. This complex network is highly specialized and the different classes of macromolecules that comprise the ECM determine its biological functi...
Article
The Cardiovascular Continuum describes a sequence of events from cardiovascular risk factors to end‐stage heart disease. It includes conventional pathologies affecting cardiovascular functions such as hypertension, atherosclerosis or thrombosis and was traditionally considered from the metabolic point of view. This Cardiovascular Continuum, origina...
Article
Full-text available
In addition to its critical role in lysosomes for catabolism of sialoglycoconjugates, NEU1 is expressed at the plasma membrane and regulates a myriad of receptors by desialylation, playing a key role in many pathophysiological processes. Here, we developed a proteomic approach dedicated to the purification and identification by LC–MS/MS of plasma m...
Article
Introduction et but de l’étude Affectant plus de 30 % de la population des pays occidentaux, la stéatose hépatique non-alcoolique (NAFLD) est la pathologie du foie la plus fréquente. Cette maladie peut conduire à de multiples complications, y compris stéato-hépatite non alcoolique (NASH), cancer, hypertension et athérosclérose. La résistance à l’in...
Article
Full-text available
Introduction et but de l’étude L’insulinorésistance, la stéatose hépatique et la dyslipidémie, caractéristiques du diabète de type 2 et de l’obésité, sont les causes principales des accidents cardiovasculaires. Parmi les facteurs décrits dans la littérature, la balance neutrophile élastase/alpha 1-antitrypsine joue un rôle important dans l’appariti...
Data
Fig. S1. Influence of EDPs on MDA‐MB‐231 cancer cell adhesion.
Data
Fig. S3. Effect of EDPs on MDA‐MB‐231 cell migration.
Data
Fig. S2. Influence of EDPs on MDA‐MB‐231 cell proliferation.
Article
Here, we cloned a new family of four adenylyl cyclase (AC) splice variants from interleukin-1β (IL-1β)-transdifferentiated vascular smooth muscle cells (VSMCs) encoding short forms of AC8 that we have named "AC8E-H". Using biosensor imaging and biochemical approaches, we showed that AC8E-H isoforms have no cyclase activity and act as dominant-negat...
Article
Full-text available
Affecting more than 30% of the Western population, nonalcoholic fatty liver disease (NAFLD) is the most common liver disease and can lead to multiple complications, including nonalcoholic steatohepatitis (NASH), cancer, hypertension, and atherosclerosis. Insulin resistance and obesity are described as potential causes of NAFLD. However, we surmised...
Article
Full-text available
Elastin is a long‐lived extracellular matrix protein responsible for the structural integrity and function of tissues. Breast cancer elastosis is a complex phenomenon resulting in both the deposition of elastotic masses and the local production of elastin fragments. In invasive human breast cancers, an increase in elastosis is correlated with sever...
Article
Full-text available
Neuraminidase 1 (NEU1) is a lysosomal sialidase catalyzing the removal of terminal sialic acids from sialyloconjugates. A plasma membrane-bound NEU1 modulating a plethora of receptors by desialylation, has been consistently documented from the last ten years. Despite a growing interest of the scientific community to NEU1, its membrane organization...
Article
Full-text available
Sialic acids (SA) are monosaccharides that can be located at the terminal position of glycan chains on a wide range of proteins. The post-translational modifications, such as N-glycan chains, are fundamental to protein functions. Indeed, the hydrolysis of SA by specific enzymes such as neuraminidases can lead to drastic modifications of protein beh...
Article
Eleven compounds were isolated from Poraqueiba sericea stems and identified as niga-ichigoside-F1 (1), trachelosperoside B1 (2), 4-epi-niga-ichigoside (7), 19α-hydroxyasiatic acid (3), myrianthic acid (4), hyptatic acid (5), trachelosperogenin B (6), arjunolic acid (8), and trachelosperogenin E (9), secologanoside (10) and secoxyloganin (11). Compo...
Poster
Initially classified as Icacinaceae family by Rusby (1896)1, a recent study based on morphological and molecular characters has demonstrated affinity of Dendrobangia boliviana with neotropical groups and was therefore transferred to the Cardiopteridaceae group2. This tree is widely distributed throughout the Neotropics, Costa Rica, Panama, Brazil a...
Article
Twelve oleanane saponins, zebiriosides A-L, were isolated from the roots of Dendrobangia boliviana Rusby, together with two known saponins, talunùmoside I and 3-O-β-d-glucuronopyranosyl serjanic acid. These saponins are glycosides of serjanic or phytolaccinic acid. Their structures were established on two basis: first, their spectral data, mainly H...
Poster
Full-text available
Sialic acids (SA) are monosaccharides that can be located at the terminal position of glycan chains on a wide range of proteins [1]. The post-translational modifications, such as N-glycan chains, are fundamental to protein functions. Indeed, the hydrolysis of SA by specific enzymes such as neuraminidases can lead to drastic modifications of protein...
Article
Cardiovascular diseases (CVD) are the leading cause of death worldwide and represent a major problem of public health. Over the years, life expectancy has considerably increased throughout the world and the prevalence of CVD is inevitably rising with the growing aging of the population. The normal process of aging is associated with progressive det...
Article
Full-text available
Elastin, one of the longest-lived proteins, confers elasticity to tissues with high mechanical constraints. During aging or pathophysiological conditions such as cancer progression, this insoluble polymer of tropoelastin undergoes an important degradation leading to the release of bioactive elastin-derived peptides (EDP), named elastokines. EDP exh...
Article
Aging is a progressive process determined by genetic and acquired factors. Among the latter are the chemical reactions referred to as nonenzymatic posttranslational modifications (NEPTMs), such as glycoxidation, which are responsible for protein molecular aging. Carbamylation is a more recently described NEPTM that is caused by the nonenzymatic bin...
Article
Full-text available
The extracellular matrix (ECM) consists of a complex set of macromolecules present in all organs and tissues and forms a three-dimensional network between cells. Considered for a long time as a naı¨ve architectural support for cells, ECM is now regarded as a dynamic structure, finely regulated between neosynthesis and degradation, and essential for...
Article
Full-text available
Degradation of elastin leads to the production of elastin-derived peptides (EDP), which exhibit several biological effects, such as cell proliferation or protease secretion. Binding of EDP on the elastin receptor complex (ERC) triggers lactosylceramide (LacCer) production and ERK1/2 activation following ERC Neu-1 subunit activation. The ability for...
Article
Full-text available
Doxorubicin (DOX), a highly active chemotherapeutic drug, faces limitations in clinical application due to severe cardiotoxic effects (mainly through increased oxidative stress). Therefore, its effect is exacerbated in subjects with ischemic heart disease. We have recently reported that saffron extract (SAF), a natural compound mainly consisting of...
Article
Full-text available
Doxorubicin (DOX) is an anthracycline antibiotic routinely used as a chemotherapeutic agent for the treatment of solid tumours. However, DOX possesses an acute and cumulative cardiotoxicity due to free radical production. The present study was designed to investigate the possible protective effects of saffron (Crocus sativus) extracts against DOX-i...
Article
Objective: Elastin is the major structural extracellular matrix component of the arterial wall that provides the elastic recoil properties and resilience essential for proper vascular function. Elastin-derived peptides (EDP) originating from elastin fragmentation during vascular remodeling have been shown to play an important role in cell physiolo...
Article
Full-text available
During melanoma tumour growth, cancerous cells are exposed to the immediate surrounding the micro- and macro environment, which is largely modified through the degradation of the extracellular matrix by fibroblast-derived metalloproteinases. Among the degradation products, (VGVAPG)3, an elastin peptide is known to stimulate the proliferation of bot...
Article
Unlabelled: In many cancers, including lung carcinomas, Fragile histidine triad (Fhit) is frequently decreased or lost. Fhit status has recently been shown to be associated with elevated in vitro and in vivo invasiveness in lung cancer. Tumor cell invasion is facilitated by epithelial-mesenchymal transition (EMT), a process by which tumor cells lo...
Article
Elastin is degraded during vascular aging and its products, elastin-derived peptides (EP), are present in the human blood circulation. EP binds to the elastin receptor complex (ERC) at the cell surface, composed of the Elastin Binding Protein (EBP), a Cathepsin A and a Neuraminidase 1. Some in vitro functions have been clearly attributed to this bi...
Conference Paper
Full-text available
Elastin is the major structural extracellular matrix component of the arterial wall that provides elastic recoil properties and resilience essential for proper tissue function. During vascular aging and in pathophysiological conditions associated with vascular inflammation such as atherosclerosis, elastin fragmentation occurs leading to the formati...
Article
Context: Anticancer treatments such as anthracyclines are effective; however, they induce cardiotoxicity by releasing radical oxygen species (ROS). Saffron (Crocus sativus; Iridaceae) is a widely used spice with antioxidant properties and numerous health benefits that may provide cardioprotection. Objective: To assess the effect of saffron again...
Article
Full-text available
Although it has long been established that the extracellular matrix acts as a mechanical support, its degradation products, which mainly accumulate during ageing, have also been demonstrated to play an important role in cell physiology and the development of cardiovascular and metabolic diseases. In the present study, we showed that elastin-derived...
Article
Full-text available
Elastin is the protein responsible for the resilience of vertebrate tissue. It is an extremely stable protein deposited during the early stages of life and experiencing almost no renewal. As a consequence, it can be considered that each individual has an elastin capital for life. Despite its extreme stability, elastin can be degraded by several enz...
Article
Full-text available
The elastin binding protein (EBP), a spliced variant of lysosomal β-galactosidase, is the primary receptor of elastin peptides that have been linked to emphysema, aneurysm and cancer progression. The sequences recognized by EBP share the XGXXPG consensus pattern found in numerous matrix proteins, notably in elastin where the VGVAPG motif is repeate...
Article
Elastin peptides constitute a group of biologically active peptides derived from the fragmentation of insoluble elastin. These molecules, currently termed elastokines, have been shown to interact preferably with the elastin receptor complex. Recent data show that the sialidase activity of the neuraminidase-1 of this receptor is required for these p...
Article
Full-text available
Leukemic cell adhesion to proteins of the bone marrow microenvironment provides signals which control morphology, motility and cell survival. We described herein the ability of ethoxyfagaronine (etxfag), a soluble synthetic derivative of fagaronine, to prevent leukemic cell adhesion to fibronectin peptide (FN/V). Phosphorylation of fak and pyk2 wer...
Article
This study aimed to investigate the degradation of the natural substrates tropoelastin and elastin by the neutrophil-derived serine proteases human leukocyte elastase (HLE), proteinase 3 (PR3) and cathepsin G (CG). Focus was placed on determining their cleavage site specificities using mass spectrometric techniques. Moreover, the release of bioacti...