Laurent Bozec

Laurent Bozec
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Laurent verified their affiliation via an institutional email.
Verified
Laurent verified their affiliation via an institutional email.
University of Toronto | U of T · Faculty of Dentistry

PhD, FRMS
follow for update @l_bozec

About

177
Publications
55,687
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Introduction
Investigating the mechano-biology of tissues at the nanoscale is paramount to establish not only the link between their forms and functions but also the inception of several diseases and disorders. With expertise in nanometrology and especially AFM & nanomechanics, Bozec’s group focuses primarily on “Mechano-Biology for Healthcare” in research fields related to aging, connective and mineralized tissue disorders, and finally bacteriology. https://discover.research.utoronto.ca/1070-laurent-bozec
Additional affiliations
September 2007 - September 2018
University College London
Position
  • Professor (Associate)
October 1999 - September 2002
Lancaster University
Position
  • PhD
January 2005 - December 2011
University College London

Publications

Publications (177)
Article
Full-text available
Background Chronic lung disease of prematurity, called bronchopulmonary dysplasia (BPD), lacks effective therapies, stressing the need for preclinical testing systems that reflect human pathology for identifying causal pathways and testing novel compounds. Alveolar organoids derived from human pluripotent stem cells (hPSC) are promising test platfo...
Article
Full-text available
Ehlers-Danlos syndromes (EDS) represent a group of rare genetic disorders affecting connective tissues. Globally, approximately 1.5 million individuals suffer from EDS, with 10,000 reported cases in Canada alone. Understanding the histological properties of collagen in EDS has been challenging, but advanced techniques like atomic force microscopy (...
Article
Purpose Physiological and erosive wear reported in clinical studies were reviewed, and in vitro aging models were developed to simulate and compare the effect of aging on human teeth with the review data obtained from clinical studies. Methods A review of clinical studies and randomized clinical trials that quantify enamel wear was performed in th...
Preprint
Full-text available
Background Chronic lung disease of prematurity, called bronchopulmonary dysplasia (BPD), lacks effective therapies, stressing the need for preclinical testing systems that reflect human pathology for identifying causal pathways and testing novel compounds. Alveolar organoids derived from human pluripotent stem cells (hPSC) are promising test platfo...
Article
Full-text available
Periodontal surgery to treat gingival recession or root exposure and improve peri-implant esthetics often requires soft tissue grafting. Allogeneic or heterologous collagen matrix can be a useful alternative to autologous soft tissue grafts via the avoidance of the invasive procedure of harvesting autologous tissues. A variety of collagen matrix pr...
Article
Full-text available
Type I collagen, a fundamental extracellular matrix (ECM) component, is pivotal in maintaining tissue integrity and strength. It is also the most prevalent fibrous biopolymer within the ECM, ubiquitous in mammalian organisms. This structural protein provides essential mechanical stability and resilience to various tissues, including tendons, ligame...
Article
Full-text available
The skin is the largest organ in the body and is essential for protecting us from environmental stressors such as UV radiation, pollution, and pathogens. As we age, our skin undergoes complex changes that can affect its function, appearance, and health. These changes result from intrinsic (chronological) and extrinsic (environmental) factors that c...
Article
The adhesion of initial colonizers such as Streptococcus mutans to collagen is critical for dentinal and root caries progression. One of the most described pathological and aging-associated changes in collagen-including dentinal collagen-is the generation of advanced glycation end-products (AGEs) such as methylglyoxal (MGO)-derived AGEs. Despite pr...
Article
Full-text available
Correction for ‘Enhanced detoxification of Cr ⁶⁺ by Shewanella oneidensis via adsorption on spherical and flower-like manganese ferrite nanostructures’ by Diana S. Raie et al. , Nanoscale Adv. , 2023, https://doi.org/10.1039/d2na00691j.
Preprint
Full-text available
While the external signs of skin aging have been well-defined throughout history, much less is known about aging within the ultrastructure of our skin. Our skin, the largest organ in our body, is structured by collagen through fibrils or large sheets. With the increased use of nanometrology tools in histology, it is now possible to explore how the...
Article
Full-text available
Maximizing the safe removal of hexavalent chromium (Cr6+) from waste streams is an increasing demand due to the environmental, economic and health benefits. The integrated adsorption and bio-reduction method can be applied for the elimination of the highly toxic Cr6+ and its detoxification. This work describes a synthetic method for achieving the b...
Article
Purpose: To investigate the effect of different in vitro aging protocols on the optical properties and crystalline structure of high-translucency zirconia. Materials and methods: Thirty-six specimens of high-translucency (HT) and extra-high translucency (XT) zirconia were divided into three groups: control (CO) - no treatment; hydrothermal aging...
Article
Full-text available
The structural and functional properties of collagen are modulated by the presence of intramolecular and intermolecular crosslinks. Advanced Glycation End-products (AGEs) can produce intermolecular crosslinks by bonding the free amino groups of neighbouring proteins. In this research, the following hypothesis is explored: The accumulation of AGEs i...
Preprint
Full-text available
Background White spot lesions (WSL) represent the earliest stage of caries formation in which mineral is lost from the enamel surface, but the surface retains its integrity. At this stage, remineralization of enamel is generally considered possible. This study aimed to develop a reliable in vitro protocol for the creation of artificially induced WS...
Preprint
Full-text available
The adhesion of initial colonizers such as Streptococcus mutans to collagen is critical for dentinal and root caries progression. One of the most described pathological and aging-associated changes in collagen, including dentinal collagen, is the generation of advanced glycation end-products (AGEs) such as methylglyoxal (MGO)-derived AGEs. Despite...
Article
Full-text available
The aim of the current work is twofold: to demonstrate the application of in situ non-invasive imaging by portable atomic force microscopy (AFM) on the surfaces of a violin and to integrate compositional and mechanical analysis at the nano scale level on model samples of varnished wood. These samples were prepared according to traditional recipes b...
Article
Background Ionizing radiation may indiscriminately and adversely affect tissues during exposure. As collagen is the most abundant protein in the human body, its exposure to ionizing radiation is inevitable. To date, there has been limited comprehensive evaluation of the biophysical properties of individual collagen fibrils exposed to clinically rel...
Article
Following pilot testing of the effects of alkaline-based nanoparticles on modern parchment, the treatment was extended to historical parchment. This paper describes the application and impact of calcium hydroxide and calcium carbonate nanoparticles dispersed in (i) propan-2-ol and (ii) cyclohexane on a parchment book cover dated 1570. The cover sho...
Preprint
Full-text available
The purpose was two-fold: To explore the capability of the Diamond Synchrotron infra-red so as to include near-field photothermal microspectroscopy (PTMS); and Toward a quantitative nanohistology - investigation of scleroderma using synchrotron radiation (mu- FTIR). With recent advances in AFM, the integration of an IR temperature-based system on a...
Article
Full-text available
Background: The early identification of children who have experienced adversity is critical for the timely delivery of interventions to improve coping and reduce negative consequences. Self-report is the usual practice for identifying children with exposure to adversity. However, physiological characteristics that signal the presence of disease or...
Preprint
Full-text available
The structural and functional properties of collagen are modulated by the presence of intramolecular and intermolecular crosslinks. Advanced Glycation End-products (AGEs) can produce intermolecular crosslinks by bonding the free amino groups of neighboring proteins. In this research, the following hypothesis is explored: The accumulation of AGEs in...
Article
Full-text available
The biophysical microenvironment of the cell is being increasingly used to control cell signalling and to direct cell function. Herein, engineered 3D tuneable biomimetic scaffolds are used to control the cell microenvironment of Adipose-derived Mesenchymal Stromal Cells (AMSC), which exhibit a collagen density-specific profile for early and late st...
Article
Methylglyoxal (MGO) is an important molecule derived from glucose metabolism with the capacity of attaching to collagen and generating advanced glycation end products (AGEs), which accumulate in tissues over time and are associated with aging and diseases. However, the accumulation of MGO-derived AGEs in dentin and their effect on the nanomechanica...
Preprint
Methylglyoxal (MGO) is an important molecule derived from glucose metabolism with the capacity of attaching to collagen and generating advanced glycation end-products (AGEs), which accumulate in tissues over time and are associated to aging and diseases. However, the accumulation of MGO-derived AGEs in dentin and their effect on the nanomechanical...
Article
Full-text available
Within the oral cavity, dental biofilms experience dynamic environments, in part due to changes in dietary content, frequency of intake and health conditions. This can impact bacterial diversity and morpho-mechanical properties. While phenotypic properties of oral biofilms are closely related to their composition, these can readily change according...
Article
Full-text available
This study aimed to assess the viability of dental cells following time-dependent carbamide peroxide teeth-whitening treatments using an in-vitro dentin perfusion assay model. 30 teeth were exposed to 5% or 16% CP gel (4 h daily) for 2-weeks. The enamel organic content was measured with thermogravimetry. The time-dependent viability of human dental...
Article
Growing interest in exploring mechanically mediated biological phenomena has resulted in cell culture substrates and 3D matrices with variable stiffnesses becoming standard tools in biology labs. However, correlating stiffness with biological outcomes and comparing results between research groups is hampered by variability in the methods used to de...
Article
Objectives Recently, the trend in treating dental caries relies on preserving the affected dentin. The success of restorations relies on remineralization of affected dentin which is very challenging. Here, we report the facile development of a dual-analogue biomimetic phosphate glass polyacrylate paste (PGPAP), combining two non-collagenous protein...
Article
Full-text available
Organoids can shed light on the dynamic interplay between complex tissues and rare cell types within a controlled microenvironment. Here, we develop gut organoid cocultures with type-1 innate lymphoid cells (ILC1) to dissect the impact of their accumulation in inflamed intestines. We demonstrate that murine and human ILC1 secrete transforming growt...
Article
Full-text available
Nanocellulose-based materials have recently been used to consolidate degraded cotton painting canvases. Canvas-supported paintings consist of materials that are sensitive to moisture and especially susceptible to environmental fluctuations in temperature and relative humidity (RH). These environmental fluctuations occur in uncontrolled environments...
Article
In article number 2000806 by Richard M. Day and co‐workers, highly porous biodegradable films are prepared using thermally‐induced phase separation. In vivo implantation of the films into ischemic tissue stimulates increased expression of pro‐angiogenic growth factor genes. This provides a favourable local environment for neovascularization, increa...
Article
Biofilm-mediated oral diseases such as dental caries and periodontal disease remain highly prevalent in populations worldwide. Biofilm formation initiates with the attachment of primary colonizers onto surfaces, and in the context of caries, the adhesion of oral streptococci to dentinal collagen is crucial for biofilm progression. It is known that...
Article
Full-text available
Peripheral arterial disease and critical limb ischemia are common symptoms of cardiovascular disease. Vascular surgery is used to create a bypass around occluded blood vessels to improve blood flow to ischemic muscle, thus avoiding the need for amputation. Attempts to vascularize tissues by therapeutic angiogenesis using delivery of exogenous angio...
Article
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Abstract In conservation, adhesives are commonly used for the consolidation of canvases, yet their impact upon the canvas longevity has raised some concerns amongst conservators. As such, this study presents a testing protocol developed to assess the performance of commonly-used adhesives (natural animal glue and synthetic Beva ® 371) and a newly d...
Preprint
Full-text available
Organoids can shed light on the dynamic interplay between complex tissues and rare cell types within a controlled microenvironment. Here, we developed gut organoid co-cultures with type-1 innate lymphoid cells (ILC1) to dissect the impact of their accumulation in inflamed intestines. We demonstrate for the first time that murine and human ILC1 secr...
Conference Paper
Full-text available
Intense pulsed light (IPL) is a high-intensity treatment for skin disorders and ageing. As this treatment regime is often poorly regulated and inadequately studied, we investigate IPL as a cosmetic device and its effects on dermal collagen components of the skin. Biopsies from the back-neck folds of a 4-week-old, 25 kg large white pig were irradiat...
Article
Full-text available
In common with many bacterial pathogens, Streptococcus pneumoniae has a polysaccharide capsule, which facilitates immune evasion and determines virulence. Recent data has shown that the closely related Streptococcus mitis also express polysaccharide capsules including those with an identical chemical structure to S. pneumoniae capsular serotypes. W...
Article
Full-text available
Introduction Dentinogenesis imperfecta type 1 (OIDI) is considered a relatively rare genetic disorder (1:5000 to 1:45,000) associated with osteogenesis imperfecta. OIDI impacts the formation of collagen fibrils in dentin, leading to morphological and structural changes that affect the strength and appearance of teeth. However, there is still a lack...
Article
Tendon mechanobiology plays a vital role in tendon repair and regeneration; however, this mechanism is currently poorly understood. We tested the role of different mechanical loads on extra‐cellular matrix (ECM) remodelling gene expression and the morphology of tendon fibroblasts in collagen hydrogels, designed to mimic native tissue. Hydrogels wer...
Article
Tendon mechanobiology plays a vital role in tendon repair and regeneration; however, this mechanism is currently poorly understood. We tested the role of different mechanical loads on extra-cellular matrix (ECM) remodelling gene expression and the morphology of tendon fibroblasts in collagen hydrogels, designed to mimic native tissue. Hydrogels wer...
Article
Full-text available
Collagen glycation, and in particular the formation of advanced glycation end-product (AGE) crosslinks, plays a central role in the ageing process and in many of the long-term complications of diabetes. Glucosepane, the most abundant and relevant AGE crosslink, has been suggested to increase the stiffness of tissue and reduce its solubility, althou...
Article
Aim: To refine FTIR protocol for detection of NaOCl-induced dentinal collagen changes using an ex vivo irrigation model, and to apply it to determine the collagen change within 0.5mm of canal or root surfaces, with or without mature roots or periodontal involvement. Methodology: Extracted human roots were irrigated with control saline (n=3) or 5...
Article
Reconstruction of a tendon rupture is surgically challenging as each end of the tendon retracts leaving a substantial gap and direct repair is often not feasible. Hence to restore function a tendon graft is required to bridge this defect and presently these gaps are filled with auto-, allo- or, synthetic grafts but they all have clinical limitation...
Preprint
Full-text available
In common with many bacterial pathogens, Streptococcus pneumoniae has a polysaccharide capsule, which facilitates immune evasion and is a key virulence determinant. However, recent data has shown that the closely related Streptococcus mitis can also express polysaccharide capsules including those with an identical chemical structure to S. pneumonia...
Article
Objective The purpose of this review was to systematically summarize the outcomes of laboratory-based studies investigated the effect of sodium hypochlorite application on bond strength of dentin to various materials. Data A comprehensive literature search was conducted using PubMed, Google Scholar, Cochrane Library and OpenThesis database. Then a...
Article
Full-text available
Carbamide peroxide (CP) is widely used as a tooth-whitening agent in self-administered tooth-bleaching products. In this study, the effects of 5% and 10% CP on dentinal collagen structure and chemical properties were evaluated in vitro. Thirty-five intact teeth were exposed to 2 whitening protocols (2 or 4 h daily) with either 5% or 10% CP gel for...
Article
Full-text available
The original version of this Article contained an error in the author affiliations. The affiliation of Marjan Enayati with ‘Ludwig Boltzmann Cluster for Cardiovascular Research at the Center for Biomedical Research, Medical University of Vienna, Austria' was inadvertently omitted. This has now been corrected in both the PDF and HTML versions of the...
Article
Full-text available
In the original version of this Article the dataset identifier in the Data Availability statement was incorrect. The correct dataset identifier is PXD009500. This has been corrected in the HTML and PDF versions of this Article.
Article
Full-text available
Modifiable hydrogels have revealed tremendous insight into how physical characteristics of cells' 3D environment drive stem cell lineage specification. However, in native tissues, cells do not passively receive signals from their niche. Instead they actively probe and modify their pericellular space to suit their needs, yet the dynamics of cells' r...
Article
Full-text available
Recent developments in paper and canvas conservation have seen the introduction of nanocellulose (NC) as a compatible treatment for the consolidation of historical cellulosic artefacts and manuscripts. However, as part of the assessment of these new materials for canvas consolidation, the adhesion of the consolidation treatment (which takes place b...
Article
Full-text available
Aim To establish a method using Fourier Transform Infra‐Red spectroscopy (FTIR) to characterise the nature and depth of changes in dentinal collagen following exposure to sodium hypochlorite (NaOCl) during root canal irrigation in an ex‐vivo model. Methodology FTIR was used to assess the changes in dentinal collagen when the root canal was exposed...
Article
Nanocellulose has been recently proposed as a novel consolidant for historical papers. Its use for painting canvas consolidation, however, remains unexplored. Here, we show for the first time how different nanocelluloses, namely mechanically isolated cellulose nanofibrils (CNF), carboxymethylated cellulose nanofibrils (CCNF) and cellulose nanocryst...
Article
Cellular function depends on the maintenance of protein homeostasis (proteostasis) by regulated protein degradation. Chronic dysregulation of proteostasis is associated with neurodegenerative and age-related diseases, and drugs targeting components of the protein degradation apparatus are increasingly used in cancer therapies. However, as chronic i...
Conference Paper
Objectives: The aim of this study was to investigate the impact of carbon supplementation of media on the microbial community and matrix constituents over an extended culture period. Methods: A fed batch culture approach utilising 96-well microtiter plates, with hydroxyapatite discs, was developed to grow microcosm dental plaque biofilms in-vitro....
Conference Paper
Full-text available
Background Three prolyl 3-hydroxylase enzymes, LEPRE1, LEPREL1 and LEPREL2, are known to modify prolines in certain sequences in the C-terminal helical region of the polypeptide chains of procollagens converting them to 3-hydroxyproline residues. This modification appears to facilitate correct alignment of the chains in forming the triple helical d...
Article
Full-text available
Over the last 5-10 years, optical coherence tomography (OCT) and atomic force microscopy (AFM) have been individually applied to monitor the morphological and mechanical properties of various single-species biofilms respectively. This investigation looked to combine OCT and AFM as a multi-scale approach to understand the role sucrose concentration...
Article
Background and objective: Although collagen membranes have been clinically applied for guided tissue/bone regeneration for more than 30 years, their in vivo degradation pattern has never been fully clarified. A better understanding of the different stages of in vivo degradation of collagen membranes is extremely important, considering that the bio...
Conference Paper
Objectives: The aim of this study was to develop a high throughput microcosm dental plaque biofilm model to investigate the bacterial composition and matrix constituents. Methods: A fed batch culture approach utilising 96-well MBEC™ plates, with hydroxyapatite coated pegs, was developed to grow dental plaque biofilms. Pooled human saliva was inocu...
Article
Full-text available
Denture-associated stomatitis is a common candidal infection that may give rise to painful oral symptoms, as well as be a reservoir for infection at other sites of the body. As poly (methyl methacrylate) (PMMA) remains the main material employed in the fabrication of dentures, the aim of this research was to evaluate the adhesion of Candida albican...
Article
Full-text available
This paper describes the application of atomic force microscopy (AFM) for the imaging of collagen denaturation as observed in parchment. Parchment is prepared from processed animal skin and collagen is the main component. Large collections in national archives, libraries and religious institutions contain numerous documents written on parchment. Th...
Article
Full-text available
The extracellular matrix of the dermis is a complex, dynamic system with the various dermal components undergoing individual physiologic changes as we age. Age-related changes in the physical properties of collagen were investigated in particular by measuring the effect of aging, most likely due to the accumulation of advanced glycation end product...