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Introduction
Lauren Engelking is a PhD student at the University of Alberta investigating nutritional management strategies to reduce postpartum inflammation in dairy cows. She has previously conducted research with dairy calves at Wageningen University and Hiroshima University as well at the University of Alberta.
Skills and Expertise
Publications
Publications (7)
The objective of this research was to assess variation in postpartum hay intake when offered alongside total mixed ration (TMR) as free choice, and identify factors related to the hay intake. Twenty multiparous cows were fed a closeup TMR (21.5% starch, 39.1% neutral detergent fiber [NDF] on a dry matter [DM] basis). After calving, cows were offere...
Dairy cattle experience inflammation during the calving transition period, and butyrate and nonsteroidal anti-inflammatory drugs (NSAID) are expected to reduce the inflammation. Our objective was to evaluate the effects of dietary butyrate supplementation and oral NSAID administration on feed intake, serum inflammatory markers, plasma metabolites,...
Recommendations for composition of oral rehydration solutions (ORS) for calves, particularly concerning Na+, glucose, and their combined effect on tonicity, are not in line with guidelines for humans. Thus, this study aimed to determine the effect of ORS tonicity on water, mineral and acid–base balance. Seventy‐two calves were selected based on the...
This study investigated how providing hay mixed with calf starter to dairy calves affected their solid feed intake, feed sorting, growth, and plasma metabolite and hormone concentrations. Forty Holstein heifer calves were fed a texturized calf starter (23.4% crude protein, 32.3% starch on a dry matter basis) and chopped Klein grass hay as separate...
Nutrition has an impact on the behavior of dairy calves in the pre- and post-weaning phases; however, an interaction between these 2 phases is poorly understood. Thus, the objective of this study was to investigate how plane of nutrition in the pre- and post-weaning phases affects feed sorting behavior post-weaning. Twenty Holstein heifer calves we...
Questions
Question (1)
Hello,
I am trying to adapt the human Calprotectin assay to use in bovines. For background, Calpotectin is a protein released by neutrophils following gastrointestinal inflammation. A couple things I am currently troubleshooting:
1)The extraction buffer I should be using: I am currently considering a citrate urea buffer with neutrophil-specific protease inhibitors. I am seeking advice on any alternative buffers or buffer components I should consider and also how I should select which protease inhibitor to include.
2) After adding the buffer to my fecal samples, I will centrifuge samples to collect supernatant: How do I determine the centrifuge settings that are appropriate for this type of assay?
Thank you in advance for any insight! Any insight is greatly appreciated.