Laure Plener

Laure Plener
Gene&GreenTK · Microbiology

PhD

About

41
Publications
12,872
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1,107
Citations
Additional affiliations
October 2015 - present
Gene&GreenTK
Position
  • Researcher
January 2012 - July 2015
Ludwig-Maximilians-University of Munich
Position
  • PostDoc Position

Publications

Publications (41)
Article
Quorum sensing (QS) is a molecular communication system used by microorganisms to adopt behaviors in a cell density-dependent manner. Lactonase enzymes, able to hydrolyze the signal molecules acyl-homoserine lactones (AHL) can counteract QS-mediated virulence in Gram-negative bacteria. Optimizing lactonases activity or specificity for AHL through e...
Article
Full-text available
Organophosphorus compounds (OPs) are neurotoxic molecules developed as pesticides and chemical warfare nerve agents (CWNAs). Most of them are covalent inhibitors of acetylcholinesterase (AChE), a key enzyme in nervous systems, and are therefore responsible for numerous poisonings around the world. Many animal models have been studied over the years...
Article
Quorum sensing (QS) is a communication system used by bacteria to coordinate a wide panel of biological functions in a cell density-dependent manner. The Gram-negative Chromobacterium violaceum has previously been shown to use an acyl-homoserine lactone (AHL)-based QS to regulate various behaviors, including the production of proteases, hydrogen cy...
Article
Full-text available
Enzymes able to degrade or modify acyl-homoserine lactones (AHL) have drawn considerable interest for their ability to interfere with the bacterial communication process referred to as Quorum Sensing (QS). Many proteobacteria use AHL to coordinate virulence and biofilm formation in a cell density-dependent manner, thus AHL-interfering enzymes const...
Article
Full-text available
The human opportunistic pathogen Pseudomonas aeruginosa orchestrates the expression of many genes in a cell density-dependent manner by using quorum sensing (QS). Two acyl-homoserine lactones (AHLs) are involved in QS circuits and contribute to the regulation of virulence factors production, biofilm formation, and antimicrobial sensitivity. Disrupt...
Article
Full-text available
Organophosphorus compounds (OP) are highly toxic molecules used as insecticides that inhibit cholinesterase enzymes involved in neuronal transmission. The intensive use of OP for vector control and agriculture has led to environmental pollutions responsible for severe intoxications and putative long-term effects on humans and wild animals. Many in...
Article
Full-text available
Many bacteria use quorum sensing (QS), a bacterial communication system based on the diffusion and perception of small signaling molecules, to synchronize their behavior in a cell-density dependent manner. QS regulates the expression of many genes associated with virulence factor production and biofilm formation. This latter is known to be involved...
Article
Quorum sensing (QS) is a molecular communication system that bacteria use to harmonize the regulation of genes in a cell density-dependent manner. In proteobacteria, QS is involved, among others, in virulence, biofilm formation or CRISPR-Cas gene regulation. Here, we report for the first time the effect of a QS-interfering enzyme to alter the regul...
Patent
La présente invention concerne l’utilisation d’une lactonase mutée appartenant à la famille des phosphotriesterase-like lactonase pour augmenter la sensibilité de bactéries aux agents anti-microbiens par rapport à l’utilisation d’agents anti-microbiens seuls.
Article
Most bacteria use a communication system known as quorum sensing which relies on the secretion and perception of small molecules called autoinducers enabling bacteria to adapt their behavior according to the population size and synchronize the expression of genes involved in virulence, antimicrobial resistance and biofilm formation. Methods have em...
Article
Many bacteria use extracellular signaling molecules to coordinate group behavior, a process referred to as quorum sensing (QS). However, some QS molecules are hydrophobic in character and are probably unable to diffuse across the bacterial cell envelope. How these molecules are disseminated between bacterial cells within a population is not yet ful...
Article
Full-text available
Numerous bacteria utilize molecular communication systems referred to as quorum sensing (QS) to synchronize the expression of certain genes regulating, among other aspects, the expression of virulence factors and the synthesis of biofilm. To achieve this process, bacteria use signaling molecules, known as autoinducers (AIs), as chemical messengers...
Article
Quorum Sensing (QS) is a communication system used by numerous bacteria to synchronize their behavior according to the cell density. In this way, bacteria secrete and sense small mediating molecules, called autoinducers (AI), which concentration increases in the environment proportionally to bacterial cell number. QS induces major physiological and...
Article
Pseudomonas aeruginosa is a Gram negative pathogenic bacterium involved in many human infections including otitis, keratitis, pneumonia, and diabetic foot ulcers. P. aeruginosa uses a communication system, referred to as quorum sensing (QS), to adopt a group behavior by synchronizing the expression of certain genes. Among the regulated traits, secr...
Article
Full-text available
Extremozymes have gained considerable interest as they could meet industrial requirements. Among these, SsoPox is a hyperthermostable enzyme isolated from the archaeon Sulfolobus solfataricus. This enzyme is a lactonase catalyzing the hydrolysis of acyl-homoserine lactones; these molecules are involved in Gram-negative bacterial communication refer...
Article
Quorum sensing (QS) is used by bacteria to communicate and synchronize their actions according to the cell density. In this way, they produce and secrete in the surrounding environment small molecules dubbed autoinducers (AIs) that regulate the expression of certain genes. The phenotypic traits regulated by QS are diverse and include pathogenicity,...
Article
Full-text available
Phages are bacteria targeting viruses and represent the most abundant biological entities on earth. Marine environments are exceptionally rich in bacteriophages, harboring a total of 4x1030 viruses. Nevertheless, marine phages remain poorly characterized. Here we describe the identification of intact prophage sequences in the genome of the marine γ...
Article
Numerous bacteria use quorum sensing (QS) to synchronize their behavior and monitor their population density. They use signaling molecules known as autoinducers (AI's) that are synthesized and secreted into their local environment to regulate QS-dependent gene expression. Among QS-regulated pathways, biofilm formation and virulence factor secretion...
Article
Full-text available
Unlabelled: Quorum sensing (QS) is a communication process that enables a bacterial population to coordinate and synchronize specific behaviors. The bioluminescent marine bacterium Vibrio harveyi integrates three autoinducer (AI) signals into one quorum-sensing cascade comprising a phosphorelay involving three hybrid sensor kinases: LuxU; LuxO, an...
Article
Full-text available
Ralstonia solanacearum is a soil-borne beta-proteobacterium that causes bacterial wilt disease in many food crops and is a major problem for agriculture in intertropical regions. R. solanacearum is a heterogeneous species, both phenotypically and genetically, and is considered as a species complex. Pathogenicity of R. solanacearum relies on the Typ...
Article
Full-text available
Author Summary While studying the potential interaction of staphylococci with Gram-negative bacteria, we came across another communication system in a Staphylococcus species group, which consists of closely related coagulase-positive bacterial species that play a role as zoonotic pathogens. We found that these species excrete two small compounds th...
Article
Full-text available
Janthinobacteria commonly form biofilms on eukaryotic hosts and are known to synthesize antibacterial and antifungal compounds. Janthinobacterium sp. HH01 was recently isolated from an aquatic environment and its genome sequence was established. The genome consists of a single chromosome and reveals a size of 7.10 Mb, being the largest janthinobact...
Data
Bacterial strains and plasmids used in this study. amp, ampicillin; gm, gentamycin; nal, nalidxin; km, kanamycin; cyc, cycloserin; tet, tetracycline. (DOCX)
Data
Predicted genes and ORFs possibly linked to cell appendages and motility in HH01. Proteins/Genes associated with Type 4 pilus assembly are in blue color. (DOCX)
Data
Survival of C. elegans in the presence of E. coli DH5α carrying extra copies of the HH01 vioA-E genes in pDrive. For the survival assay 30 L4 larvae were placed onto agar plates. The worms were transferred onto new plates every day and incubated at 20°C. Alive and dead worms were counted during transfer. The treatment groups were violacein expressi...
Data
Primers used for cloning and mutant construction. (DOCX)
Data
Predicted Genes/ORFs linked to resistance mechanisms in HH01. (DOCX)
Data
Genes/ORFs linked to protein secretion. (DOCX)
Data
ORFs and genes predicted in the HH01 genome. This file contains the submission list of the Janthinobacterium sp. HH01 genome. The corresponding GenBank files are available at: DDBJ/EMBL/GenBank access.ion AMWD00000000. Genes/ORFs on contig 1 are indicated with Jab_1cxxxx. Genes/ORFs on contig 2 are indicated with Jab_2cxxxx (XLSX)
Data
V. cholerae ΔcqsA mutant cannot be complemented by the HH01 jqsA. A) The enhanced biofilm formation phenotype of a V. cholerae ΔcqsA strain cannot be reverted by provision of jqsA in trans. The indicated V. cholerae strains were incubated statically within 24-well plates and biofilm formation was scored after 24 hours of growth using a standard cry...
Article
Full-text available
Quorum sensing regulates cell density-dependent phenotypes and involves the synthesis, excretion and detection of so-called autoinducers. Vibrio harveyi strain ATCC BAA-1116 (recently reclassified as Vibrio campbellii), one of the best-characterized model organisms for the study of quorum sensing, produces and responds to three autoinducers. HAI-1,...
Article
Full-text available
MetE and MetH are two distinct enzymes that catalyze a similar biochemical reaction during the last step of methionine biosynthesis, MetH being a cobalamin-dependent enzyme whereas MetE activity is cobalamin-independent. In this work, we show that the last step of methionine synthesis in the plant pathogen Ralstonia solanacearum is under the transc...
Data
Growth of the wild-type strain GMI1000 and methionine synthase mutants on minimal medium plates supplemented with methionine 25 µM, 3.7 µM or in absence of methionine. (TIF)
Data
MetE and MetH are cobalamin-independent and –dependent enzymes, respectively. (TIF)
Article
Bacterial wilt (brown rot) disease of potato caused by Ralstonia solanacearum is one of the most important bacterial diseases and a major constraint on potato production worldwide. Through a comparative genomic analysis between R. solanacearum'race 3 biovar 2' (R3bv2) strains, we identified a 77 kb region in strain UW551 which is specifically absen...
Article
Full-text available
The ability of Ralstonia solanacearum to cause disease in plants depends on its type III secretion system (T3SS). The expression of the T3SS and its effector substrates is coordinately controlled by a regulatory cascade, at the bottom of which is HrpB. Transcription of the hrpB gene is activated by a plant-responsive regulator named HrpG, which is...

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