Laure Menin

Laure Menin
École Polytechnique Fédérale de Lausanne | EPFL · Institute of Chemical Sciences and Engineering

phD

About

97
Publications
10,271
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2,851
Citations
Citations since 2016
66 Research Items
1549 Citations
2016201720182019202020212022050100150200250300
2016201720182019202020212022050100150200250300
2016201720182019202020212022050100150200250300
2016201720182019202020212022050100150200250300

Publications

Publications (97)
Preprint
Hypervalent iodine reagents have recently emerged as powerful tools for late-stage peptide and protein functionalization. Herein we report a tyrosine bioconjugation methodology for the introduction of hypervalent iodine onto biomolecules under physiological conditions. Tyrosine residues were engaged in a selective addition onto the alkynyl bond of...
Article
Continuous fluidic sampling systems allow collection of brain biomarkers in vivo. Here, we propose a new sequential and intermittent sampling paradigm using droplets, called Droplet on Demand (DoD). It is implemented in a microfabricated neural probe and alternates phases of analyte removal from the tissue and phases of equilibration of the concent...
Article
The time-domain transients in the Fourier transform mass spectrometry (FTMS) analysis of monoclonal antibodies (mAbs) are known to exhibit characteristic isotopic beat patterns. These patterns are defined by the isotopic distributions of all gaseous mAb ions present in the FTMS mass analyzer, originating from single or multiple charge states, and f...
Preprint
Full-text available
Continuous fluidic sampling systems allow collection of brain biomarkers in vivo . Here, we propose a new sampling paradigm, Droplet on Demand (DoD), implemented in a microfabricated neural probe. It allows sampling droplets loaded with molecules from the brain extracellular fluid punctually, without the long transient equilibration periods typical...
Article
Full-text available
Monoclonal antibodies (mAbs) are protein biotherapeutics with a proven efficacy toward fighting life-threatening diseases. Their exceptional healing potential drives the annual increase in the number of novel mAbs and other antibody-like molecules entering clinical trials and the number of approved mAb-based drugs. Mass spectrometry (MS) offers hig...
Article
Full-text available
Although genomic DNA is the primary target of anticancer platinum-based drugs, interactions with proteins also play a significant role in their overall activity. In this study, competitive binding of cisplatin with an oligonucleotide and two peptides corresponding to segments of H2A and H2B histone proteins was investigated by mass spectrometry. Fo...
Article
Full-text available
Hypervalent iodine reagents have recently emerged as powerful tools for late-stage peptide and protein functionalization. Herein we report a tyrosine bioconjugation methodology for the introduction of hypervalent iodine onto biomolecules...
Article
Full-text available
The billion tons of synthetic‐polymer‐based materials (i.e. plastics) produced yearly are a great challenge for humanity. Nature produces even more natural polymers, yet they are sustainable. Proteins are sequence‐defined natural polymers that are constantly recycled when living systems feed. Digestion is the protein depolymerization into amino aci...
Article
Full-text available
Protein Recycling In article number 2104581, Sebastian Josef Maerkl, Francesco Stellacci, and co-workers introduce an idealized representation of nature-inspired circular-economy recycling (NaCRe) for proteins, a recycling approach where generic mixtures of protein-based materials are depolymerized into their constituent amino acids, which in turn...
Article
Full-text available
The circadian clock is a ubiquitous molecular time-keeping mechanism which synchronizes cellular, tissue, and systemic biological functions with 24-hour environmental cycles. Local circadian clocks drive cell type- and tissue-specific rhythms and their dysregulation has been implicated in pathogenesis and/or progression of a broad spectrum of disea...
Preprint
Full-text available
Traditionally, mass spectrometry (MS) output is the ion abundance plotted versus ionic mass-to-charge ratio m/z. While employing only commercially available equipment, Charge Determination Analysis (CHARDA) adds a third dimension to MS, estimating for individual peaks their charge states z, starting from z=1, and colour-coding z in m/z spectra. CHA...
Preprint
Traditionally, mass spectrometry (MS) output is the ion abundance plotted versus ionic mass-to-charge ratio m/z. While employing only commercially available equipment, Charge Determination Analysis (CHARDA) adds a third dimension to MS, estimating for individual peaks their charge states z, starting from z=1, and colour-coding z in m/z spectra. CHA...
Article
Full-text available
The therapeutic efficacy and pharmacokinetics of antibody–drug conjugates (ADCs) in general, and antibody–oligonucleotide conjugates (AOCs) in particular, depend on the drug-to-antibody ratio (DAR) distribution and average value. The DAR is considered a critical quality attribute, and information pertaining to it needs to be gathered during ADC/AOC...
Article
Full-text available
Early-diverging fungi (EDF) are distinct from Dikarya and other eukaryotes, exhibiting high N6-methyldeoxyadenine (6mA) contents, rather than 5-methylcytosine (5mC). As plants transitioned to land the EDF sub-phylum, arbuscular mycorrhizal fungi (AMF; Glomeromycotina) evolved a symbiotic lifestyle with 80% of plant species worldwide. Here we show t...
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Full-text available
Annonaceae family has broad uses in herbal medicine for treatment of several diseases, whether through seeds' or leaves' extracts. The present study investigates the antiproliferative and antitumor activity of Annona cher-imola aqueous leaf (AAL) extract/infusion in acute myeloid leukemia (AML) cell lines in vitro. High-resolution LC-MS was first u...
Preprint
Full-text available
In 2070, 10 ¹² Kg of polymer-based materials ( i.e. plastics) might be produced yearly, posing one of the greatest challenges that humanity has to face. Even though natural polymers, such as proteins and nucleic acids, are more abundant than synthetic ones, they are sustainable. The key property of such natural polymers is that they are sequence-de...
Article
RATIONALE The Analysis of Oligonucleotide Modifications from Mass Spectra (Aom2s) was created to support the analysis of oligonucleotide mass spectra. This application complements the existing software tools by providing a comprehensive analysis of oligonucleotide fragments from HR‐MS/MS data in a flexible and user‐friendly manner, being directly a...
Article
Fourier transform mass spectrometry (FTMS) applications require accurate analysis of extremely complex mixtures of species in a wide mass and charge state ranges. To optimize the related FTMS data analysis accuracy, parameters for data acquisition and the allied data processing should be selected rationally and their influence on the data analysis...
Article
Full-text available
The Consortium for Top-Down Proteomics (www.topdownproteomics.org) launched the present study to assess the current state of top-down mass spectrometry (TD MS) and middle-down mass spectrometry (MD MS) for characterizing monoclonal antibody (mAb) primary structures, including their modifications. To meet the needs of the rapidly growing therapeutic...
Article
Full-text available
Bile acids, synthesized from cholesterol by the liver, are chemically transformed along the intestinal tract by the gut microbiota and the products of these transformations signal through host receptors, impacting overall host health. These transformations include bile acid deconjugation, oxidation, and 7α-dehydroxylation. An understanding of the b...
Article
Full-text available
The design of stimuli-responsive nanocarriers has raised much attention to achieve higher local concentration of therapeutics and mitigate the appearance of drug resistance. The combination of imaging properties and controlled photorelease of active molecules within the same nanoconjugate has a great potential for theranostic applications. In this...
Article
Full-text available
Cisplatin [ cis- diamminedichloroplatinum(II) ( cis -DDP)] is one of the most successful anticancer agents effective against a wide range of solid tumors. However, its use is restricted by side effects and/or by intrinsic or acquired drug resistance. Here, we probed the role of glutathione transferase (GST) P1-1, an antiapoptotic protein often over...
Article
Full-text available
Oviparous animals across many taxa have evolved diverse strategies that deter egg predation, providing valuable tests of how natural selection mitigates direct fitness loss. Communal egg laying in nonsocial species minimizes egg predation. However, in cannibalistic species, this very behavior facilitates egg predation by conspecifics (cannibalism)....
Data
High-resolution 10 T APPI FT-ICR MS. Mass spectra of the wax-layer hexane extract of D. melanogaster eggs (A, G, M) and the hexane control solution (D, J, P). The expanded views of mass regions demonstrate the presence of (B) 11Z,11-octadecen-1-ol-acetate (C20H38O2) and (C) 7Z,11Z-tricosadiene (C23H44) compounds in the hexane extract and their abse...
Data
High-resolution 10 T APPI FT-ICR MS of hexane extract of wax layer of eggs laid by transgenic mutant fly crosses with/without ablated oes. C1, ♂oe+ × ♀oe+; C2, ♂oe− × ♀oe−; C3, ♂oe− × ♀oe+; and C4, ♂oe+ × ♀oe−. The table shows the intensities (SNRs) of four major (the highest abundance) peaks (ion types) of the target compound identified in the cor...
Data
Egg leakage in eggs laid by oe mutants and hexane-washed eggs perfumed with individual pheromones. (A) Pictorial representation of the role of maternal hydrocarbons present within the egg shell in preventing leakage of egg contents. Labels across each row represent eggs laid by transgenic mutant flies with ablated oes (oe−) that were either self-cr...
Data
Pheromone profile of intact and dechorinated eggs and repeated hexane washes of dechorinated eggs. (A) GC-MS profile of hexane extract of intact (S1) and dechorinated (S2) eggs indicating the peaks corresponding to the four major hydrocarbons (cVA; 7-T; 7,11-HD, and 7,11-ND). (Inset) the relative amount of these hydrocarbons in hexane extract of in...
Data
High-resolution 10 T APPI FT-ICR MS of commercially synthesized hydrocarbons and eggs laid by oe mutants. (A) Mass spectra of commercially synthesized hydrocarbons diluted in hexane. The mass spectra show four major (the highest abundance) ions of 11Z,11-octadecen-1-ol-acetate (cVA; C20H38O2); 7Z-tricosene (C23H48); 7,11Z-heptacosadiene (C27H52); a...
Data
Role of larval chemosensory receptors in sensing 7,11-HD. (A) Proportion (mean ± SE) of hexane-washed (white bars) eggs perfumed with 7,11-HD that were cannibalized by Canton S and ppk23 mutant larvae (ANOVA: n = 4 replicates, 10 eggs/replicate). (B) Proportion (mean ± SE) of dechorinated (dark bars) and hexane-washed (light bars) eggs cannibalized...
Data
High-resolution 10 T APPI FT-ICR MS. The compounds (alkenes and alkadienes) were identified in mass spectrum of wax layer’s hexane extract of intact eggs of wild-type flies. Only the monoisotopic peak (12C) of the highest abundance ion of an identified compound is indicated in the table. APPI FT-ICR MS, atmospheric pressure photoionization Fourier...
Data
Raw data for figures in supplementary information. (XLSX)
Data
Control bioassays for various experiments. (A, B) Effect of removing egg layers on egg development: (A) egg hatching success (mean ± SE), (B) egg developmental period (mean ± SE), and (C) egg-to-adult viability (mean ± SE) of D. melanogaster eggs that were intact, dechorinated, and hexane washed after dechorination (n = 5 replicate vials). Hexane t...
Article
Full-text available
The human gut hosts trillions of microorganisms that exert a profound influence on human biology. Gut bacteria communicate with their host by secreting small molecules that can signal to distant organs in the body. Bile acids are one class of these signaling molecules, synthesized by the host and chemically transformed by the gut microbiota. Among...
Article
Pairing light and heavy chains in monoclonal antibodies (mAbs) using top-down (TD) mass spectrometry (MS) may complement the sequence information on single chains provided by high-throughput genomic sequencing and bottom-up proteomics, favoring the rational selection of drug candidates. The 50 kDa F(ab) subunits of mAbs are the smallest structural...
Article
Using easy-to-access metalloligands, we were able to prepare Pd 6 L 8 -type coordination cages of unprecedented size.
Article
Full-text available
Carotenoids are organic pigment molecules that play important roles in signalling, control of oxidative stress, and immunity. Fish allocate carotenoids to their eggs, which gives them the typical yellow to red colouration and supports their resistance against microbial infections. However, it is still unclear whether carotenoids act mainly as a shi...
Data
Summary of the average concentrations (nM) of the measured carotenoids in embryo samples per maternal half sib family and treatment with associated standard deviations (SD) and coefficients of variation (CV). Data represent technical replicates (two independent runs of the same sample at different times during ultrahigh-performance liquid chromatog...
Data
Relationship between larval traits. Means per families are shown for the control group (white circles, dotted lines) vs. the PF treatment (black circles, solid lines) for hatching time vs. (a) hatchling length, (b) yolk sac volume at hatching, and (c) larval growth. See Table 3 for statistics. (PDF)
Data
Proportional changes in carotenoid content relative to early fitness-related traits. Embryo hatching time (a–c), hatchling length (d–f), yolk sac volume at hatching (g–i), and larval growth (j–l) are shown for change in astaxanthin, lutein, and zeaxanthin. Changes in carotenoid contents are given for sham-treated controls (circles and solid lines)...
Data
The three carotenoid measurements per female. Carotenoids were quantified in eggs before fertilization (triangles), at the late-eyed development stage in the control treatment (crossed diamonds) and in the PF treatment (filled diamonds) for (a) astaxanthin, (b) lutein and (c) zeaxanthin. Data on the eggs come from Wilkins et al. [24]. (PDF)
Data
Statistical leverage relative to standardized residuals of each female in linear models on embryo performance, including the outlier female. Panels represent data of models on the effects of treatment, change in lutein, and their interaction on (a) hatching time, (b) hatchling length, (c) larval growth, and (d) yolk sac volume at hatching. Each fem...
Data
Proportional changes in carotenoid content relative to early fitness-related traits. Hatchling length (a–c) and yolk sac volume at hatching (d–f) are shown for change in astaxanthin, lutein, and zeaxanthin. Changes in carotenoid contents are given for sham-treated controls (circles and solid lines) and PF treated samples (triangles and dashed lines...
Preprint
Full-text available
Carotenoids are organic pigment molecules that play important roles in signalling, control of oxidative stress, and immunity. Fish allocate carotenoids to their eggs, which gives them the typical yellow to red colouration and supports their resistance against microbial infections. However, it is still unclear whether carotenoids act mainly as a shi...
Article
Full-text available
Life-history theory predicts that iteroparous females allocate their resources differently among different breeding seasons depending on their residual reproductive value. In iteroparous salmonids there is typically much variation in egg size, egg number, and in the compounds that females allocate to their clutch. These compounds include various ca...
Article
Full-text available
Metallodrug-protein interactions contribute to their therapeutic effect (even when DNA is the dominant target), side-effects and are implicit in drug resistance. Here, we provide mass spectrometric-based evidence to show that metallodrug interactions with proteins are considerably more complex than current literature would suggest. Using native inc...
Article
Full-text available
Lückenhafte Ligandenverteilungen: Die morphologische Entwicklung einer Ligandenschale im Laufe von Ligandenaustauschreaktionen auf Nanopartikeloberflächen wird zum ersten Mal untersucht. Kinetik und Thermodynamik spielen zusammen eine wichtige Rolle bei der Bildung von lückenhaften Domänen in verschiedenen Reaktionsstadien. Abstract Ligand exchang...
Article
Full-text available
Herein we show that oxaliplatin reacts rapidly with DMSO in aqueous solutions, despite being stable in pure DMSO and pure water. Furthermore, the reactivity of the clinically applied Pt(ii) drugs in water/DMSO and PBS/DMSO mixtures, and the nature of the species formed were investigated by MS, NMR and RP-HPLC techniques.
Article
RAPTA compounds, ([Ru(η⁶-arene)(PTA)X2], PTA = 1,3,5-triaza-7-phosphaadamantane), have been reported to overcome drug resistance in cisplatin resistant cells. However, the exact mechanism of these complexes is still largely unexplored. In this study, the interaction of some RAPTA compounds with the N-terminal region of the BRCA1 RING domain protein...
Article
Full-text available
Bile acids, important mediators of lipid absorption, also act as hormone-like regulators and as antimicrobial molecules. In all these functions their potency is modulated by a variety of chemical modifications catalyzed by bacteria of the healthy gut microbiota, generating a complex variety of secondary bile acids. Intestinal commensal organisms ar...
Article
Full-text available
Social insects frequently engage in oral fluid exchange – trophallaxis – between adults, and between adults and larvae. Although trophallaxis is widely considered a food-sharing mechanism, we hypothesized that endogenous components of this fluid might underlie a novel means of chemical communication between colony members. Through protein and small...
Data
Hemolymph and trophallactic fluid Juvenile hormone titers for 20 pooled samples of each fluid.DOI: http://dx.doi.org/10.7554/eLife.20375.013
Data
A table of all orthologous proteins and their predicted functions, identifiers, known D. melanogaster orthologs, presence of annotated secretion signals and average NSAF values when present in TF.A complete orthology across the four species can be found in Supplementary file 3.DOI: http://dx.doi.org/10.7554/eLife.20375.018
Data
Cross-correlation matrix for panels 3D-E.DOI: http://dx.doi.org/10.7554/eLife.20375.009
Data
Head-width measurements for panel 4C.DOI: http://dx.doi.org/10.7554/eLife.20375.015
Data
Proteomic experiments included in this study. DOI: http://dx.doi.org/10.7554/eLife.20375.021
Data
Orthology matrix across four social insect species. DOI: http://dx.doi.org/10.7554/eLife.20375.022
Data
Peak lists for cuticular and trophallactic fluid long chain hydrocarbons form GC-MS experiments for five different colonies of C.floridanus.C11, 20 ants, 9.5 µL of TF; C12, 38 ants, 20 µL; C13, 34 ants, 11 µL; C14, 26 ants, 11.5 µL; C15, 35 ants, 9 µL.DOI: http://dx.doi.org/10.7554/eLife.20375.008
Data
Juvenile hormone titers for 37 individual third instar larvae.DOI: http://dx.doi.org/10.7554/eLife.20375.014
Data
Metamorphosis or death counts for panel 4D.DOI: http://dx.doi.org/10.7554/eLife.20375.016
Data
RNA of microorganisms present in trophallactic fluid. DOI: http://dx.doi.org/10.7554/eLife.20375.020