Laure-Emmanuelle Zaragosi

Laure-Emmanuelle Zaragosi
Université Côte d'Azur · Institut de Pharmacologie Moléculaire et Cellulaire (IPMC/UMR6097 CNRS-UNS)

About

106
Publications
12,760
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5,824
Citations
Citations since 2016
48 Research Items
4961 Citations
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201620172018201920202021202205001,0001,500
201620172018201920202021202205001,0001,500

Publications

Publications (106)
Preprint
Full-text available
Organ- and body-scale cell atlases have the potential to transform our understanding of human biology. To capture the variability present in the population, these atlases must include diverse demographics such as age and ethnicity from both healthy and diseased individuals. The growth in both size and number of single-cell datasets, combined with r...
Preprint
Full-text available
Organ- and body-scale cell atlases have the potential to transform our understanding of human biology. To capture the variability present in the population, these atlases must include diverse demographics such as age and ethnicity from both healthy and diseased individuals. The growth in both size and number of single-cell datasets, combined with r...
Article
Full-text available
The emergence and quick spread of SARS-CoV-2 has pointed at a low capacity response for testing large populations in many countries, in line of material, technical and staff limitations. The traditional RT-qPCR diagnostic test remains the reference method and is by far the most widely used test. These assays are limited to a few probe sets, require...
Article
A finely regulated system of airway epithelial development governs the differentiation of motile ciliated cells of the human respiratory tract, conferring the body’s mucociliary clearance defence system. Human cilia dysfunction can arise through genetic mutations and this is a cause of debilitating disease morbidities that confer a greatly reduced...
Preprint
Full-text available
The emergence and quick spread of SARS-CoV-2 has pointed at a low capacity response for testing large populations in many countries, in line of material, technical and staff limitations. The traditional RT-qPCR diagnostic test remains the reference method and is by far the most widely used test. These assays are limited to a couple of probe sets, r...
Article
Full-text available
Rationale: The respiratory tract constitutes an elaborated line of defense that is based on a unique cellular ecosystem. Single-cell profiling methods enable the investigation of cell population distributions and transcriptional changes along the airways. Methods: We have explored the cellular heterogeneity of the human airway epithelium in 10 heal...
Preprint
Full-text available
Mammary carcinoma, including triple-negative breast carcinomas (TNBC) are tumor-types for which human and canine pathologies are closely related at the molecular level. Low-passage, primary carcinoma cells from TNBC versus non-TNBC were used to compare the efficacy of an oncolytic vaccinia virus (VV). We show that non-TNBC cells are 28 times more s...
Article
Full-text available
We investigated SARS-CoV-2 potential tropism by surveying expression of viral entry-associated genes in single-cell RNA-sequencing data from multiple tissues from healthy human donors. We co-detected these transcripts in specific respiratory, corneal and intestinal epithelial cells, potentially explaining the high efficiency of SARS-CoV-2 transmiss...
Article
Full-text available
There is pressing urgency to understand the pathogenesis of the severe acute respiratory syndrome coronavirus clade 2 (SARS-CoV-2) which causes the disease COVID-19. SARS-CoV-2 spike (S)-protein binds ACE2, and in concert with host proteases, principally TMPRSS2, promotes cellular entry. The cell subsets targeted by SARS-CoV-2 in host tissues, and...
Article
Full-text available
The respiratory tract is lined by a pseudo-stratified epithelium from the nose to terminal bronchioles. This first line of defense of the lung against external stress includes five main cell types: basal, suprabasal, club, goblet and multiciliated cells, as well as rare cells such as ionocytes, neuroendocrine and tuft/brush cells. At homeostasis, t...
Preprint
Full-text available
Rationale The respiratory tract constitutes an elaborated line of defense based on a unique cellular ecosystem. Single-cell profiling methods enable the investigation of cell population distributions and transcriptional changes along the airways. Methods We have explored cellular heterogeneity of the human airway epithelium in 10 healthy living vo...
Article
Full-text available
The upper airway epithelium, mainly composed of multiciliated, goblet, club and basal cells, ensures proper mucociliary function and can regenerate upon aggressions. In chronic airway diseases, defective repair leads to tissue remodeling. Delineating key drivers of differentiation dynamics can help understand how normal or pathological regeneration...
Preprint
This protocol provides details on the cell dissociation that should be performed to obtain single-cell suspensions from airway biopsies. Biopsies may come from tracheal, bronchial or nasal epithelium. Cell dissociation is performed at 4°C to avoid gene expression alterations and maximize viability. The typical cell number recovery is 40 000 cells f...
Article
Full-text available
Multiciliated cells (MCCs) harbor dozens to hundreds of motile cilia, which generate hydrodynamic forces important in animal physiology. In vertebrates, MCC differentiation involves massive centriole production by poorly characterized structures called deuterosomes. Here, single-cell RNA sequencing reveals that human deuterosome stage MCCs are char...
Preprint
Full-text available
Background: It is usually considered that the upper airway epithelium is composed of multiciliated, goblet, secretory and basal cells, which collectively constitute an efficient first line of defense against inhalation of noxious substances. Upon injury, regeneration of this epithelium through proliferation and differentiation can restore a proper...
Preprint
This protocol provides details on the cell dissociation that should be performed to obtain single-cell suspensions from nasal epithelium brushings. Cell dissociation is performed at 4°C to avoid gene expression alterations and maximize viability. The typical cell number recovery is 200 000 - 300 000 for one brushing. Cell suspensions are suitable f...
Preprint
This protocol provides details on the cell dissociation that should be perform to obtain single-cell suspensions from nasal epithelium brushings. Cell dissociation is performed at 4°C to avoid gene expression alterations and maxiize viability. The typical cell number recovery is 200 000 - 300 000 for one brushing. Cell suspensions are suitable for...
Preprint
Full-text available
Multiciliated cells (MCCs) harbour dozens to hundreds of motile cilia, which beat in a synchronized and directional manner, thus generating hydrodynamic forces important in animal physiology. In vertebrates, MCC differentiation critically depends on the synthesis and release of numerous centrioles by specialized structures called deuterosomes. Litt...
Article
Full-text available
There is a growing body of evidence about the presence and the activity of the miRISC in the nucleus of mammalian cells. Here, we show by quantitative proteomic analysis that Ago2 interacts with the nucleoplasmic protein Sfpq in an RNA-dependent fashion. By a combination of HITS-CLIP and transcriptomic analyses, we demonstrate that Sfpq directly co...
Article
Full-text available
The amyloid precursor protein (APP) harbors physiological roles at synapses and is central to Alzheimer’s disease (AD) pathogenesis. Evidence suggests that APP intracellular domain (AICD) could regulate synapse function, but the underlying molecular mechanisms remain unknown. We addressed AICD actions at synapses, per se, combining in vivo AICD exp...
Data
Statistics. (A) AICD increases NMDAR, but not AMPAR, transmission in CA1 pyramidal neurons. (B) AICD regulates synaptic currents mediated by increasing synaptic GluN2B contribution. (C) Statistics (AICD regulates GluN2B mRNA levels). (D) APP knock down eliminates synaptic GluN2B NMDARs, an effect rescued by nuclear AICD delivery. (E) Physiological...
Data
Table S1. miRNA expression and sequence distribution in ten tissues (Study SRP006043).
Data
Table S2. First sheet: Gene expression profiling obtained in RNA sequencing after 72‐h overexpression of individual miR mimics (negative control miR‐Neg, canonical miR‐34a/b/c or miR‐449a/b/c, 5′‐isomiR‐34b or 5′‐isomiR‐449c) in proliferating primary HAECs (GSE77251) and analyzed using the edger package V 3.14.0 (see Materials and Methods).
Article
Full-text available
MiR-34/449 microRNAs are conserved regulators of multiciliated cell differentiation. Here, we evidence and characterize expression of two isomiR variant sequences from the miR-34/449 family in human airway epithelial cells. These isomiRs differ from their canonical counterparts miR-34b and miR-449c by one supplemental uridine at their 5'-end, leadi...
Article
Full-text available
Experimental evidence indicates that about 60% of miRNA-binding activity does not follow the canonical rule about the seed matching between miRNA and target mRNAs, but rather a non-canonical miRNA targeting activity outside the seed or with a seed-like motifs. Here, we propose a new unbiased method to identify canonical and non-canonical miRNA-bind...
Article
Full-text available
Single cell RNA sequencing approaches are instrumental in studies of cell-to-cell variability. 5' selective transcriptome profiling approaches allow simultaneous definition of the transcription start size and have advantages over 3' selective approaches which just provide internal sequences close to the 3' end. The only currently existing 5' select...
Article
Full-text available
Data from next generation sequencing technologies uncovered the existence of many classes of small RNAs. Recent studies reported that small RNAs are released by cells and can be detected in the blood. In this report, we aimed to discover the occurrence of novel circulating small RNAs in coronary artery disease (CAD).Methods We used high-throughput...
Article
Full-text available
Vertebrate multiciliated cells (MCCs) contribute to fluid propulsion in several biological processes. We previously showed that microRNAs of the miR-34/449 family trigger MCC differentiation by repressing cell cycle genes and the Notch pathway. Here, using human and Xenopus MCCs, we show that beyond this initial step, miR-34/449 later promote the a...
Article
Full-text available
Despite the importance of mucociliary epithelia in animal physiology, the mechanisms controlling their establishment are poorly understood. Using the developing Xenopus epidermis and regenerating human upper airways, we reveal the importance of BMP signalling for the construction of vertebrate mucociliary epithelia. In Xenopus, attenuation of BMP a...
Article
Full-text available
Syndecan-1 (SDC1/CD138) is one of the main cell surface proteoglycans and is involved in crucial biological processes. Only a few studies have analyzed the role of SDC1 in mesenchymal tumor pathogenesis. In particular, its involvement in adipose tissue tumors has never been investigated. Dedifferentiated liposarcoma, one of the most frequent types...
Article
Full-text available
ABCC4 is a member of the ATP-binding cassette transporter family known to transport prostaglandin E2 and other molecules across cellular membranes. A mutation in ABCC4 is now shown to cause defects in ciliogenesis, revealing a role for prostaglandin signalling in regulating cilia dynamics.
Chapter
L’adipogenèse est généralement décrite comme un processus en deux étapes. La première étape consiste en la génération de progéniteurs d’adipocytes (PA) à partir de cellules souches. La seconde étape est la différenciation terminale de ces progéniteurs en adipocytes matures. La différenciation des PA en adipocytes a été largement étudiée in vitro [1...
Article
Full-text available
Specificity of interaction between a microRNA (miRNA) and its targets crucially depends on the seed region located in its 5'-end. It is often implicitly considered that two miRNAs sharing the same biological activity should display similarity beyond the strict six nucleotide region that forms the seed, in order to form specific complexes with the s...
Chapter
Full-text available
The current epidemic of obesity and overweight has caused a surge of interest in the study of adipose tissue formation. Much progress has been made in defining the transcriptional networks controlling the terminal differentiation of preadipocytes into mature adipocytes. However, the earliest steps that direct mesenchymal stem cells located in adipo...
Data
Overlap between predicted targets of miR-210 and miR-147 family members. A) In silico evaluation of the common predicted targets between hsa-miR-210, hsa-miR-147a and hsa-miR-147b using TargetScan or microCosm. B) Overlap between the predicted targets for each of the 3 miRNAs that are significantly down-regulated following transfection by each of t...
Data
List of forward and reverse primers used for cloning 3′UTR sequences in pSI-CHECKTM-2 (Promega) by cloning behind the Renilla luciferase in the XhoI and NotI restrictions sites. (DOCX)
Data
List of themes corresponding to “Molecular Function” annotations identified by Ingenuity Pathway Analysis in response to overexpression of hsa-miR-210, hsa-mir-147a or hsa-miR-147b. The probability to obtain the number of genes in a certain pathway in the list of differentially expressed genes was compared with the representation of the same pathwa...
Data
Usage of distinct seed (2–7 nt) sequences among human miRNAs. Pie-chart showing the representation of seed 2–7 among all human mature miRNAs. On the 872 distinct human seed sequences in miRBase v16, 665 are unique and 207 are shared by two or more miRNAs (miR). MiRNAs sharing the same seed sequence can belong to distinct miRBase families, thus the...
Data
Full list of the predicted targets transcripts down-regulated following hsa-miR-210, hsa-miR-147a or hsa-miR-147b overexpression in A549 cells. The transcripts predicted to be targets (seed 2–7 in 3′UTR) are highlited. Logarithm (base 2) of the average expression, logarithm (base 2) of the ratio of miR-210/miR-Neg and false discovery rate p-values...
Data
Association of microRNAs and miRBase family with each human seed. In miRBase v16, 872 distinct seed sequences were found in human microRNA. For each seed, the number of microRNA having this seed is reported (count_mirna) along with the number and names of microRNA having this seed in the 5′ arm (count_mirna_5p, mirna_5p) or the 3′ arm (count_mirna_...
Data