Laure Burel-Deschamps

• Université de Bretagne Occidentale

Lipo-phosphoramidates constitute an interesting class of synthetic vectors for gene delivery. A structure–activity relationship study points out that the incorporation of the linoleyl polyunsaturated lipid chains produces efficient vectors for gene delivery in the lung. In addition, neutral lipo-phosphoramidates possessing an imidazole motif in the...

The synthesis of cationic lipo-thiophosphoramidates, a new family of cationic lipids designed for gene delivery, is reported herein. This new class of lipids is less polar than its oxygenated equivalent the lipo-phosphoramidates. Fluorescence anisotropy and FRET were used to determine the fluidity and fusogenicity of the lipo-phosphoramidates 3a-b...

Cationic lipophosphoramidates constitute a class of cationic lipids we have previously reported to be efficient for gene transfection. Here, we synthesized and studied a novel lipophosphoramidate derivative characterized by an arsonium headgroup linked, via a phosphoramidate linker, to an unconventional lipidic moiety consisting of two diunsaturate...

We report here a study on the physicochemical properties of cationic phospholipids liposomes used for lipoplex formulation and DNA transfer. The original cationic phospholipids synthesized in our laboratory are first presented with the liposome formulation process. The second part deals with the liposomes fusogenic properties studied by fluorescenc...

The behaviour of three South African coals in water and, particularly in seawater, was examined. A sequential speciation procedure used to evaluate trace metal partitioning in coal has shown that trace metals will not be easily released from these coals into environmental ecosystems. Only a few trace elements are slightly leached from these coals i...

Whenever immersed in seawater after a collier accident, a fossil fuel such as coal could become a source of pollution to the marine environment. To study the effect of such a contamination, four coal samples from different origins were used. A first analysis on those coals enabled us to determine the content of polycyclic aromatic hydrocarbons. Sea...

The DNA compacting properties of polyamines (especially spermine) are well-known, hence the use of spermine as the cationic part in several synthetic DNA carriers. Here, we describe the synthesis of modified spermines, with a "lipophosphoramidate" as the lipidic part, and their use for efficient in vitro transfection. Physicochemical measurements (...

The absorption and fluorescence spectra of carbaryl (CB), carbofuran (CF) and carbendazim (MBC) have been studied. Fluorescence lifetime and fluorescence quantum yields are also reported as well as the influence of pH, solvent and presence of humic acids on fluorescence. The limit of detection (LD) of the three compounds has been measured by direct...

This paper presents a synthesis of some analytical potentialities of an equipment designed for both laser induced molecular and atomic fluorescence in the field of ultra-trace analysis (ng l(-1)). Excitation of fluorescence was performed with a pulsed Nd:Yag laser coupled to an optical parametric oscillator (OPO). Fluorescence spectra were recorded...

Fluorescence was induced by coupling a laser with an optical parametric oscillator (OPO) to develop an analytical method for the direct determination of lead content, at ultra-trace level, in seawater by electrothermal atomization-laser-excited atomic fluorescence (ETA-LEAF). The optimization of atomization conditions, laser pulse energy, and mainl...

The development of an analytical method for the direct determination of ultra trace content of aluminum in seawater by electrothermal atomization-laser excited atomic fluorescence (ETA-LEAF) using a pulsed Nd:Yag laser associated with an optical parametric oscillator (OPO) and an intensified CCD (ICCD) camera is described. The good temporal reprodu...

This study deals with the optimisation and application of a method for direct analysis of trace pollutants in water by laser-induced fluorescence. The arrangement used consisted of an Nd:YAG Laser coupled with an optical parametric oscillator (LYOPO) and connected to a spectrophotometer and a high-sensitivity camera. Optimisation was achieved by de...