Laure Bally-Cuif

Laure Bally-Cuif
French National Centre for Scientific Research | CNRS · Institut de Neurobiologie Alfred Fessard

About

156
Publications
19,885
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
9,519
Citations
Citations since 2016
28 Research Items
3955 Citations
20162017201820192020202120220200400600
20162017201820192020202120220200400600
20162017201820192020202120220200400600
20162017201820192020202120220200400600

Publications

Publications (156)
Article
Full-text available
Background Quantitative imaging of epithelial tissues requires bioimage analysis tools that are widely applicable and accurate. In the case of imaging 3D tissues, a common preprocessing step consists of projecting the acquired 3D volume on a 2D plane mapping the tissue surface. While segmenting the tissue cells is amenable on 2D projections, it is...
Article
Full-text available
Neural stem cell (NSC) populations persist in the adult vertebrate brain over a lifetime, and their homeostasis is controlled at the population level through unknown mechanisms. Here, we combine dynamic imaging of entire NSC populations in their in vivo niche over several weeks with pharmacological manipulations, mathematical modeling, and spatial...
Preprint
Full-text available
B ackground Quantitative imaging of epithelial tissues prompts for bioimage analysis tools that are widely applicable and accurate. In the case of imaging 3D tissues, a common post-processing step consists in projecting the acquired 3D volume on a 2D plane mapping the tissue surface. Indeed, while segmenting the tissue cells is amenable on 2D proje...
Article
Full-text available
Adult stem cell populations must coordinate their own maintenance with the generation of differentiated cell types to sustain organ physiology, in a spatially controlled manner and over long periods. Quantitative analyses of clonal dynamics have revealed that, in epithelia, homeostasis is achieved at the population rather than at the single stem ce...
Preprint
Neural stem cell (NSC) populations persist in the adult vertebrate brain over a life time, and their homeostasis is controlled at the population level. The nature and properties of these coordination mechanisms remain unknown. Here we combine dynamic imaging of entire NSC populations in their in vivo niche over weeks, pharmacological manipulations,...
Article
Full-text available
Adult neurogenesis, i.e., the generation of neurons from neural stem cells (NSCs) in the adult brain, contributes to brain plasticity in all vertebrates. It varies, however, greatly in extent, location and physiological characteristics between species. During the last decade, the teleost zebrafish (D. rerio) was increasingly used to study the molec...
Article
Full-text available
The cellular basis and extent of neural stem cell (NSC) self-renewal in adult vertebrates, and their heterogeneity, remain controversial. To explore the functional behavior and dynamics of individual NSCs, we combined genetic lineage tracing, quantitative clonal analysis, intravital imaging, and global population assessments in the adult zebrafish...
Article
Full-text available
Today, 3D imaging techniques are emerging, not only as a new tool in early drug discovery but also for the development of potential therapeutics to treat disease. Particular efforts are directed towards in vivo physiology to avoid perturbing the system under study. Here, we assess non-invasive 3D lensless imaging and its impact on cell behavior and...
Article
Full-text available
Although developmental mechanisms driving an increase in brain size during vertebrate evolution are actively studied, we know less about evolutionary strategies allowing accelerated brain growth. In zebrafish and other vertebrates studied to date, apical radial glia (RG) constitute the primary neurogenic progenitor population throughout life [1]; t...
Preprint
Full-text available
While developmental mechanisms driving increase in brain size during vertebrate evolution are actively studied, we know less of evolutionary strategies allowing to boost brain growth speed. In zebrafish and other vertebrates studied to date, radial glia (RG) constitute the primary neurogenic progenitor population throughout life (Kriegstein and Alv...
Preprint
The cellular basis and extent of neural stem cell (NSC) self-renewal in adult vertebrates, and their heterogeneity, remain controversial. To explore the functional behavior and dynamics of individual NSCs within brain germinal pools, we combined genetic lineage tracing, quantitative clonal analysis, intravital imaging and global population assessme...
Preprint
Full-text available
While the vertebrate brain appears largely bilaterally symmetrical in humans, it presents local morphological Left-Right (LR) asymmetries as, for instance, in the petalia. Moreover, higher functions such as speech or handedness are asymmetrically localized in the cortex. How these brain asymmetries are generated remains unknown. Here, we reveal a s...
Data
Supplementary Table 1: Calculation of the habituation coefficient: example of Lphn3-CO larvae. Supplementary Table 2: Calculation of the adjusted percentage of change in locomotion: example on Lphn3-CO larvae treated with Apo. Supplementary Table 3: Examples of experimental and adjusted values for 24 control-injected animals treated with Apo and an...
Article
Full-text available
Neural stem cells (NSCs) in the adult vertebrate brain are found in a quiescent state and can preserve long-lasting progenitor potential (stemness). Whether and how these two properties are linked, and to what extent they can be independently controlled by NSC maintenance pathways, is unresolved. We previously identified Notch3 signaling as a major...
Article
Full-text available
Polymorphisms in the gene coding for the adhesion G-protein coupled receptor LPHN3 are a risk factor for attention-deficit/hyperactivity disorder (ADHD). Transient down-regulation of latrophilin3.1 (lphn3.1), the zebrafish LPHN3 homologue, causes hyperactivity. Zebrafish injected with a lphn3.1-specific morpholino are hyperactive and display an imp...
Data
Movie S2. Rotational View of the Brain Slice Shown in Figure S6A, Related to Figure 5
Article
Full-text available
Spatiotemporal variations of neurogenesis are thought to account for the evolution of brain shape. In the dorsal telencephalon (pallium) of vertebrates, it remains unresolved which ancestral neurogenesis mode prefigures the highly divergent cytoarchitectures that are seen in extant species. To gain insight into this question, we developed genetic t...
Chapter
Attention-deficit/hyperactivity disorder (ADHD) is a common, early onset neuropsychiatric disorder that is characterized by developmentally inappropriate inattention, hyperactivity, increased impulsivity and motivational/emotional dysregulation. However, although there is a significant genetic component to ADHD, relatively few risk genes have been...
Data
Document S1. Supplemental Experimental Procedures, Figures S1–S7, and Tables S1–S4
Article
Full-text available
Throughout life, adult neural stem cells (NSCs) produce new neurons and glia that contribute to crucial brain functions. Quiescence is an essential protective feature of adult NSCs; however, the establishment and maintenance of this state remain poorly understood. We demonstrate that in the adult zebrafish pallium, the brain-enriched miR-9 is expre...
Article
Full-text available
Neurogenesis in the post-embryonic vertebrate brain varies in extent and efficiency between species and brain territories. Distinct neurogenesis modes may account for this diversity, and several neural progenitor subtypes, radial glial cells (RG) and neuroepithelial progenitors (NE), have been identified in the adult zebrafish brain. The neurogenic...
Chapter
Brain development must be precisely regulated to ensure the correct final assembly of neuronal networks. Subtle alterations in neuro-developmental processes have been associated with a variety of mental disorders. MicroRNAs (miRNAs) are fine regulators of gene networks and play key roles in the successive steps of neuro-development. Their discovery...
Article
Full-text available
MicroRNAs (miRNAs) play a major role in the post-transcriptional regulation of target genes, especially in development and differentiation. Our understanding about the transcriptional regulation of miRNA genes is limited by inadequate annotation of primary miRNA (pri-miRNA) transcripts. Here, we used CAGE-seq and RNA-seq to provide genome-wide iden...
Article
Full-text available
Protein-coding mutations in the transcription factor-encoding gene ARX cause various forms of intellectual disability (ID) and epilepsy. In contrast, variations in surrounding non-coding sequences are correlated with milder forms of non-syndromic ID and autism and had suggested the importance of ARX gene regulation in the etiology of these disorder...
Article
Full-text available
Enhancers can regulate the transcription of genes over long genomic distances. This is thought to lead to selection against genomic rearrangements within such regions that may disrupt this functional linkage. Here we test this concept experimentally using the human X chromosome. We describe a scoring method to identify evolutionary maintenance of l...
Article
Radial glial progenitors play pivotal roles in the development and patterning of the spinal cord, and their fate is controlled by Notch signaling. How Notch is shaped to regulate their crucial transition from expansion toward differentiation remains, however, unknown. miR-132 in the developing zebrafish dampens Notch signaling via a cascade involvi...
Article
Little is known on the embryonic origin and related heterogeneity of adult neural stem cells (aNSCs). We use conditional genetic tracing, activated in a global or mosaic fashion by cell type-specific promoters or focal laser uncaging, coupled with gene expression analyses and Notch invalidations, to address this issue in the zebrafish adult telence...
Article
Full-text available
Soon after its discovery, microRNA-9 (miR-9) attracted the attention of neurobiologists, since it is one of the most highly expressed microRNAs in the developing and adult vertebrate brain. Functional analyses in different vertebrate species have revealed a prominent role of this microRNA in balancing proliferation in embryonic neural progenitor po...
Article
microRNA are small non-coding RNA that modulate gene expression post-transcriptionally. Discovered 20 years ago, their individual functions start to be unraveled. Collectively, functional studies point to an important functional plasticity of microRNA, along the course of evolution, and across different cellular contexts. This is the case in partic...
Article
Full-text available
βB2-crystallin (gene symbol: Crybb2/CRYBB2) was first described as a structural protein of the ocular lens. This gene, however, is also expressed in several regions of the mammalian brain, although its function in this organ remains entirely unknown. To unravel some aspects of its function in the brain, we combined behavioral, neuroanatomical, and...
Article
Full-text available
Zebrafish exhibit remarkable alterations in behaviour and morphology as they develop from early larval stages to mature adults. In this study we compare the locomotion parameters of six common zebrafish strains from two different laboratories to determine the stability and repeatability of these behaviours. Our results demonstrate large variability...
Data
Photographs of casper and AB zebrafish in our behavioural setup. Although casper mutant zebrafish are more transparent than AB wild-types, they were both readily detectable by the Zebralab programme. Comparison of adult AB and casper fish at 1 month (A,B) and 3 months (C,D). (TIF)
Data
Schematic representation of the 6 dpf, 1 month and 3 month behavioural setup. At 6 dpf larvae were measured in 24-well plate in a ZebraBox. At one month the fish were individually placed in a small box (9.5 cm×6 cm×4.5 cm) inside a ViewPoint ZebraCube. At 3 months mature fish were placed in an AquaBox 3 and then positioned in a large home-made cham...
Article
Maintaining the homeostasis of germinal zones in adult organs is a fundamental but mechanistically poorly understood process. In particular, what controls stem cell activation remains unclear. We have previously shown that Notch signaling limits neural stem cell (NSC) proliferation in the adult zebrafish pallium. Combining pharmacological and genet...
Article
Full-text available
Zebrafish (Danio rerio) are rapidly gaining popularity in translational neuroscience and behavioral research. Physiological similarity to mammals, ease of genetic manipulations, sensitivity to pharmacological and genetic factors, robust behavior, low cost, and potential for high-throughput screening contribute to the growing utility of zebrafish mo...
Article
Full-text available
Serotonin is a monoamine neurotransmitter that is involved in numerous physiological functions and its dysregulation is implicated in various psychiatric diseases. In all non-placental vertebrates, serotoninergic (5-HT) neurons are present in several regions of the brain, including the hypothalamus. In placental mammals, however, 5-HT neurons are l...
Article
Full-text available
Molecular Psychiatry publishes work aimed at elucidating biological mechanisms underlying psychiatric disorders and their treatment
Article
The timing of commitment and cell-cycle exit within progenitor populations during neurogenesis is a fundamental decision that impacts both the number and identity of neurons produced during development. We show here that microRNA-9 plays a key role in this process through the direct inhibition of targets with antagonistic functions. Across the vent...
Article
Full-text available
Small fresh water fishes such as the zebrafish (Danio rerio) have become important model organisms for biomedical research. They currently represent the best vertebrate embryo models in which it is possible to derive quantitative data on gene expression, signaling events, and cell behavior in real time in the living animal. Relevant phenotypes in f...
Article
Full-text available
Attention-deficit/hyperactivity disorder (ADHD) is a neurodevelopmental disorder characterized by inattention, hyperactivity, increased impulsivity and emotion dysregulation. Linkage analysis followed by fine-mapping identified variation in the gene coding for Latrophilin 3 (LPHN3), a putative adhesion-G protein-coupled receptor, as a risk factor f...
Article
Reactive glia, including astroglia and oligodendrocyte progenitors (OPCs) are at the core of the reaction to injury in the mammalian brain with initially beneficial and later partially adverse functions such as scar formation. Given the different glial composition in the adult zebrafish brain with radial ependymoglia but no parenchymal astrocytes,...
Article
We show that mutation of fibroblast growth factor receptor 1a simultaneously increases aggression, boldness and exploration in adult zebrafish. Reduction of Fgf signalling is correlated with a reduction of histamine levels in the mutant brain. Pharmacological increase of histaminergic signalling rescues the behavioural phenotype. This work demonstr...
Article
Regulation of corticotropin-releasing hormone (CRH) activity is critical for the animal's adaptation to stressful challenges, and its dysregulation is associated with psychiatric disorders in humans. However, the molecular mechanism underlying this transcriptional response to stress is not well understood. Using various stress paradigms in mouse an...
Article
Widely used in biomedical research, zebrafish (Danio rerio) are steadily gaining popularity as a model organism for studying neurobehavioral phenomena. Here, we focus on to the growing spec-trum of zebrafish behavioral phenotypes and the 'bigger' biological problems these models help to address. Emphasizing the developing potential of zebrafish as...
Article
Full-text available
Widely used in biomedical research, zebrafish (Danio rerio) are steadily gaining popularity as a model organism for studying neurobehavioral phenomena. Here, we focus on to the growing spectrum of zebrafish behavioral phenotypes and the ‘bigger’ biological problems these models help to address. Emphasizing the developing potential of zebrafish as a...
Article
Behavioral syndromes are suites of two or more behaviors that correlate across environmental contexts. The aggression-boldness syndrome links aggression, boldness, and exploratory activity in a novel environment. Although aggression-boldness has been described in many animals, the mechanism linking its behavioral components is not known. Here we sh...
Article
All subdivisions of the adult zebrafish brain maintain niches of constitutive neurogenesis, sustained by quiescent and multipotent progenitor populations. In the telencephalon, the latter potential neural stem cells take the shape of radial glia aligned along the ventricle and are controlled by Notch signalling. With the aim of identifying new mark...
Data
Table S3. List of the sequences used for the molecular phylogeny (Figure 1) and their genomic locations.
Data
Figure S2. her13 expression highlights early neurogenesis domains during zebrafish embryonic development. Whole-mount in situ hybridization for her13 (blue staining) at the stages indicated (A-C and E are lateral views, D and F are dorsal views, all with anterior to the left). Note her13 expression in proneural clusters encompassing presumptive spi...
Data
Figure S3. Neural plate patterning is unaffected upon blocking Her8a function. Whole-mount in situ hybridization for barhl2 (A,B), her5 (C,D) and her9 (E,F) in embryos injected with her8a capped mRNA (right column) compared to control embryos (left column). Dorsal views of whole-mount embryos are shown, anterior to the top. All three markers highli...
Data
Table S1. Summary of yeast two-hybrid results. The 75 unique protein-protein interactions recovered are listed, ordered according to their interaction scores (PBS: Protein Binding Score). The recovered her genes are underlined.
Data
Figure S4. her8aMO1 and her8aMO2 have identical effects on neurog1 expression. Whole-mount in situ hybridization for neurog1 expression in embryos injected with her8aMO1 (B) or her8aMO2 (C) compared to control embryos (whole-mount views of 3 somite-embryos, anterior to the top). neurog1 expression is ectopically induced between the clusters of moto...
Data
Table S2. Gene Ontology enrichment analysis. This analysis was conducted on the recovered yeast-2-hybrid candidates from the categories A, B and C.
Data
Full-text available
Figure S1. Alignment of mouse (Mm) Hes1, Hes5 and Hes6 with Hes6-like proteins from zebrafish (Dr) (Her8a, Her8.2, Her13 and Hes6) and other species (Fr: Fugu rubripes, Ol: Oryzias latipes, Gg: Gallus gallus, Hs: Homo sapiens, Xt: Xenopus tropicalis). Protein domains are color-coded: green: basic domain, purple: HLH domain, orange: orange domain. N...