Laura Kragie

BioMedWorks LLC · Executive

Aromatase enzyme is a cytochrome P450 protein that can catalyze the aromatization of C 19 androgens and thus their conversion into estrogens. It is tissue-specifically expressed in primate placenta, connective tissue, ovary, and brain through alternative splicing of tissue selective promoters. Human aromatase activity is critical for maintenance of...

Unlabelled: Human aromatase (CYP19) converts C19 androgens to aromatic C18 estrogenic steroids. Its activity is critical for early and mid pregnancy maintenance and in regulating parturition in late pregnancy. Past studies have utilized placental microsome tritiated water release assay to assess drug-hormone interactions with estrogen synthesis. W...

Gender differences after acute cocaine administration have received little attention in spite of the fact that males and females respond differently to many drugs. Seven male and seven female occasional cocaine users received both an intranasal dose of cocaine hydrochloride (0.9 mg/kg) and placebo powder in a randomized order and reported subjectiv...

T3 cellular uptake is inhibited in the presence of benzodiazepines (BZs). The structure-activity relationship of BZ inhibition correlates strongly with halogen substitution of the nonfused phenyl ring and indicates that this ring is required for activity. A structure-activity series of thyromimetic (TH) inhibitors of the HepG2 iodothyronine transpo...

Treating elderly debilitated patients with symptomatic bradyarrhythmia may be difficult. Traditional therapy includes use of limited resources (intensive care unit monitoring) and expensive interventions (permanent pacemaker insertion). When a pacemaker is not ideal or appropriate, yet intervention is desired to improve quality of life, theophyllin...

A novel method and composition for treating vitamin B12 deficiency mammals that fail to respond to oral vitamin B12 therapy.

Just as the Rainbow connects Heaven and Earth, you can link Spiritual Practice and Mainstream Medicine. This book provides you with a cognitive scaffold that facilitates your mental ability to integrate Alternative Healing methods with those from Evidence-Based Medicine, thus allowing for the peaceful coexistence of the irrational with the rational...

http://www.epa.gov/endo/pubs/aromatase_peer_review_012208.pdf

1. Understand the process of tissue generated estrogen from circulating androgen precursors/prohormones and consequences of inhibiting the in situ production of estrogen, in men, women, fetus and child. 2. Learn about in vitro screening tools for endocrine disruptors of estrogenic production/receptor cascade, and the EPA’s Endocrine Disruptor Scree...

This disclosure describes compositions and methods of use of compositions, that can replace the role of estrogens in the functions of humans and other animals, when these humans or animals are under the influence of compounds, devices and biologics that can inhibit the activity of aromatase enzyme (estrogen synthetase). https://www.google.com/paten...

Human aromatase (CYP19) converts C19 androgens to aromatic C18 estrogenic steroids that are important for pregnancy maintenance and also in regulating uterine contractions in late pregnancy. Inhibitors of this enzyme are associated with fetal loss in non-human primates. We tested a panel of azole antifungals which may be administered to pregnant wo...

Food and Drug Administration's Office of Women's Health, in collaboration with the FDA's Center for Drug Evaluation and Research, the Center for Biologics Evaluation and Research, and the Center for Devices and Radiological Health, convened a public workshop entitled "Gender Studies in Product Development: Scientific Issues and Approaches." The mee...

Journal cover illustration featuring article

Hypothyroidism is associated with muscle weakness and slowed movements. Hormone-deficient muscles do show changes in contractile proteins and sarcoplasmic calcium pumps, however effects on the components of the neuromuscular junction are less clear. In this study, we examined cholinesterase activity in adult fast-twitch muscle from hypothyroid and...

T3 cellular uptake is inhibited in the presence of benzodiazepines (BZs). The structure-activity relationship of BZ inhibition correlates strongly with halogen substitution of the nonfused phenyl ring and indicates that this ring is required for activity. A structure-activity series of thyromimetic (TH) inhibitors of the HepG2 iodothyronine transpo...

We measured the density and affinity of the peripheral benzodiazepine receptor (PBR) ligand, [3H]4'-Cl-diazepam, in cardiac ventricular and liver homogenates from thyroidectomized (TX) Holtzman adult male rats, and compared these data to sham-operated controls. When data from hypothyroid tissues were compared to those of controls, the densities of...

The purpose of this review is to incorporate new research developments implying a direct interaction of thyroid hormones (TH) and benzodiazepines (BZ) at the cellular level, into our understanding of the neuroendocrinologic mechanisms involved in neuropsychiatric disorders. General information pertaining to TH endocrinology and BZ pharmacology is p...

Thyroid hormone deficiency is associated with changes in the cardiovascular system. No one has reported the measures of both atrial and ventricular calcium channel density and function, in association with measures of negative and positive channel modulators, in hypothyroid hearts. Hormonally-induced modulation of calcium channels has clinical sign...

Hypothyroidism frequently presents with muscle complaints. No consistent histopathology nor electrophysiology explains these symptoms and signs. As well, no previous study shows specific changes in components of the nerve-muscle synapse nor excitation-contraction coupling in adult muscles, but changes are seen in hormone-treated embryonic myoblasts...

L-T3 (T3) accumulates into cells in a temperature-dependent saturable manner through a purported iodothyronine membrane carrier protein. We report energy-dependent uptake of picomolar [125I]T3 into differentiated cell lines derived from human liver, human neuroblast, and rat pituitary malignancies. Furthermore, this cellular uptake is inhibited by...

Previously, we reported potent inhibition of triiodo-L-thyronine (T3) cellular uptake into a human liver cell line (HepG2) by central and peripheral receptor specific benzodiazepine (BZ) compounds and our working hypothesis of BZ's as direct competitors for the iodothyronine transporter, displacing T3 but not acting as a substrate for transport. In...

In vitro and in situ tests have been run to characterize the reaction of the mettalochromic indicator, arsenazo III, with calcium. Job plots as well as plots of indicator absorbance vs. [Ca2+] at different indicator concentrations show a 1:1 reaction stoichiometry. Equilibrium analysis and analysis using Adair's equation are also consistent with 1:...