Laura Guimarães

University of Porto | UP · Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR)

As a highly sensitive vibrational technique, Raman spectroscopy (RS) can provide valuable chemical and molecular data useful to characterise animal cell types, tissues and organs. As a label-free, rapid detection method, RS has been considered a valuable asset in forensics, biology and medicine. The technique has been applied to zebrafish for vario...

Background: Pesticide usage has several beneficial impacts on agricultural production. However, it also has negative environmental impacts on soil, water, and non-target species, which can potentially cause a decrease in biodiversity. A good example is the decline of bee populations due to pesticide use. Fenpy-roximate (FEN) is a fungicide that act...

Background: Tramadol (TRA) and its main metabolites are among the most detected pharmaceutical compounds in aquatic ecosystems, reaching concentrations in the µg/L range. In humans, TRA acts on different receptors of the noradrenergic, serotoninergic, dopaminergic and opioid systems. Also, it is me-tabolized into the active metabolite o-desmethyltr...

This work presents an engaging hands-on activity designed to teach school students about nature based solutions (NBS) and their role in achieving high-quality water systems within a sustainable circular economy. Through this activity, students actively participate in building a small constructed wetland (CW) and test its effectiveness under various...

Simple Summary Over the years, research from other areas has suggested that tadpole shrimps could be a useful model to detect and evaluate the effects of aquatic contamination and environmental health. Its size, active swimming, short generation time, and easy lab maintenance make it an attractive alternative to the use of higher sentient animals....

Triops longicaudatus is a crustacean typically inhabiting temporary freshwater bodies in regions with a Mediterranean climate. These crustaceans are easily maintained in the laboratory and show a set of biological features that make them good candidates for diagnosing environmental quality and health. However, information about their responses to e...

Tributyltin (TBT) is a biocide introduced in the 1960s in antifouling paints. Despite legislation banning its use, its persistence in the environment still causes significant harm to organisms. Tributyltin is a ligand of retinoid X receptors (RXR) and ecdysteroid receptors (EcRs), which in arthropods act as homologs of RXR. Focusing on Metazoan spe...

Pharmaceutical compounds and their metabolites are found in natural and wastewater. However, investigation of their toxic effects on aquatic animals has been neglected, especially for metabolites. This work investigated the effects of the main metabolites of carbamazepine, venlafaxine and tramadol. Zebrafish embryos were exposed (0.1-100 µg/L) for...

Pharmaceuticals and pesticides are recognized micropollutants in freshwater systems. Their ever-increasing frequency of detection, levels found and little information available about their effects on non-target organisms, make them emerging contaminants. However, parental compounds are not the only substances of concern. Their metabolites and degra...

The common use of tires is responsible for the production of large quantities of waste worldwide, which are landfilled or energetically recovered, with higher economical cost and known environmentally harmful consequences. This type of problem must be studied, and all efforts must be conducted to eliminate, or at least mitigate, such high costs. Th...

(1) An approach with great potential for fast and cost-effective profiling and identification of diatoms in lake ecosystems is presented herein. This approach takes advantage of Raman spectroscopy. (2) The study was based on the analysis of 790 Raman spectra from 29 species, belonging to 15 genera, 12 families, 9 orders and 4 subclasses, which were...

Antibiotics are often applied in aquaculture to prevent fish diseases. These substances can cause disturbances on receiving waters, when not properly eliminated from the aquaculture effluents. In this work, ozone (O3) was investigated as a possible oxidizing agent to remove fishery antibiotics from aquaculture effluents: florfenicol (FF), oxytetrac...

The occurrence of psychopharmaceuticals in aquatic ecosystems is a growing problem. Fluoxetine (FL) and its metabolite norfluoxetine (NF) are selective serotonin reuptake inhibitors. Although they may be potentially harmful to non-target species, available knowledge on the effects of NF is sparse, relative to FL. This study aimed at contributing to...

Motivation theory is indispensable when discussing processes of learning. Learners who are motivated can learn almost everything. Students' motivation is probably one of the most important factors for teacher effectiveness both for engagement in the learning process and high academic performance. To have effective environmental education, it is not...

Freshwater quality has been changing due to the ever greater use of water resources and the contamination load resulting from human activities. Management of these systems, thus, requires constant diagnose of water quality with fast and efficient methodologies. The conventional methods adopted are, however, time-consuming, often very expensive, and...

Dear Colleagues, The application of biomarkers in ecotoxicology and biomonitoring has been rapidly accelerating since the 90s due to their many advantages, including in allowing for expeditious determination. This, however, has resulted in frequent misleading interpretations, mostly due to i) their application without any underlying effects-based...

This study aimed at assessing the effects of the dietary tryptophan (Trp) supplementation on growth and feed utilization, brain serotonin content, and expression of selected liver genes (involved in the liver serotonin pathway, protein synthesis degradation, and antioxidant activity) in zebrafish. A growth trial was conducted with zebrafish juvenil...

Low levels of concern about anthropogenic climate change have been attributed to a range of factors, some of which relate to education. These include people’s lack of understanding and engagement with the multifaceted nature and extent of the problem that it presents to current and future generations. Limited knowledge is also known to be an obstac...

The last two decades produced a wealth of work revealing the pressing need to increase ocean literacy in support of ocean sustainability and governance. The aim of this chapter is to present the Portuguese Blue School programme, an initiative born in response to the above need, to prepare ocean-literate citizens and support the development of the b...

Contamination by microplastics is increasing steadily worldwide, affecting all environments. Additionally, aquatic organisms are often exposed to mixtures of other contaminants, including various chemicals. Numerous studies reported adsorption of chemicals to microplastics, raising concern about their possible role as vehicles of exposure through t...

Diatom species are good pollution bioindicators due to their large distribution, fast response to changes in environmental parameters and different tolerance ranges. These organisms are used in ecological water assessment all over the world using autoecological indices. Such assessments commonly rely on the taxonomic identification of diatom specie...

Contamination derived from maritime transportation is not only linked to petroleum-based substances but also to transportation of other substances such as hazardous and noxious nature (HNS). Over the last decade, the maritime trade of HNS has been augmenting. However, levels of HNS in aquatic environment are still quite unknown. So, an integrative...

Maritime trade and transportation of hazardous and noxious substances (HNS) have been increasing in European waters, augmenting the risk of accidental spills from ships or in harbours. Despite their reported toxicity and hazardousness, information on HNS levels in the aquatic environment is still lacking. Therefore, an assessment combining a chemic...

The data presented herein relates to the article entitled “Norfluoxetine and venlafaxine in zebrafish larvae: single and combined toxicity of two pharmaceutical products relevant for risk assessment” [1]. Recent studies have shown the occurrence of active metabolites of human and veterinary pharmaceuticals in surface and wastewaters. Besides their...

Levonorgestrel is one of the active ingredients of oral contraceptives detected in the aquatic environment at concentrations in the order of ng/L. During the past decade, a wealth of new information about levonorgestrel has been produced, with several studies having reported negative effects in the reproduction and growth of aquatic organisms after...

Antidepressant metabolites are found in natural and waste waters. However, investigation of their toxic effects on aquatic animals, single or in mixture with other occurring psychoactive drugs, has been neglected. Here, effects of 80hpf exposure to norfluoxetine (0.64-400 ng/L), venlafaxine (16-10000 ng/L) or their combination (3.2 ng/L +2000 ng/L,...

Aquaculture is among the industries growing at the fastest rate in the world. This industry has been recognized to play a critical role in food production for a continuously expanding world population. However, despite various technological innovations and improvements in production techniques, this sector is still associated with misperceptions an...

Marine litter awareness hands on actions through learning by doing (MALIA) is a two year funded Erasmus+ project, started in January 2018 that aims to include marine conservation practices and marine litter educational guidelines in the national school curriculum from 4 EU countries (Portugal, Cyprus, Italy and Spain). To enhance intercultural comp...

Rivers are amongst the most threatened ecosystems in Europe. To prevent further degradation and to improve their ecological status, effective mitigation and restoration actions are needed. Those actions are primarily based on the precision of the ecological assessment results. This study aims to assess the ecological status of two small Mediterrane...

Indices based on community structure provide a global ecological status of the biological communities but they are of limited value as early-warning indicators of contamination. Almost two decades after the Water Framework Directive (2000/60/EC) was adopted, we reviewed publications that measured biomarkers in benthic macroinvertebrates in biomonit...

Biomarkers are recognised sensitive early-warning tools of biological effects in aquatic organisms. In this scope, the main aim of this study was to investigate the potential usefulness of a battery of biomarkers, evaluated in different benthic macroinvertebrate taxa, to discriminate aquatic ecosystems with different levels of ecological status and...

Marine litter awareness hands-on actions through learning by doing (MALIA), is a project funded by Erasmus+ programme carried out in Portugal, Cyprus, Italy and Spain. It started in January 2018 and aims at including marine conservation practices and marine litter educational guidelines in the national school curriculum enhancing students and teach...

In the present study, the underlying short-term effects of gold nanoparticles (AuNP, spheres, citrate coated, ∼40 nm) on the hepatic function of gilthead sea bream (Sparus aurata) was assessed, using a species-specific enriched oligonucleotide microarray platform (SAQ). Two distinct concentrations of AuNP (0.5 and 50 μg/L) were tested during 24 h w...

Lymnaea stagnalis, also referred to as great or common pond snail, is an abundant and widespread invertebrate species colonizing temperate limnic systems. Given the species importance, studies involving L. stagnalis have the potential to produce scientifically relevant information, leading to a better understanding of the damage caused by aquatic c...

Urban groundwater is a core resource to human health, and well-being, decisive for sustainable development. Extreme weather events and active release of man-made chemicals are increasing groundwater scarcity and loss of quality around the world. Surface and subsurface release of chemical contaminants are among the most compromising activities. Grou...

The data presented herein relates to the article entitled "Multibiomarker interactions to diagnose and follow-up chronic exposure of a marine crustacean to hazardous and noxious substances (HNS)" (Abreu et al., 2018). Multibiomarker approaches, including molecular, biochemical, physiological and behaviour parameters, are recognised as valuable and...

Integrated compensatory responses of physiological systems towards homeostasis are generally overlooked when it comes to analysing alterations in biochemical parameters indicative of such processes. Here an hypothesis-driven multivariate analysis accounting for interactive multibiomarker responses was used to investigate effects of long-term exposu...

The growing economic interest in the exploitation of mineral resources on deep-ocean beds, including those in the vicinity of sensitive-rich habitats such as hydrothermal vents, raise a mounting concern about the damage that such actions might originate to these poorly-know ecosystems, which represent millions of years of evolution and adaptations...

Manifestations of climate change can be found in subtle shifts in environmental conditions, such as sea level rise, melting glaciers or changing oceanic acidity due to atmospheric CO 2 uptake. Over the past two decades, a great deal of media production has focused on informing the public about scientific discoveries about the health of the ocean an...

The aim of the present study was to test biomarker responses in an edible mollusk, Donax trunculus L. (Mollusca, Bivalvia) associated with environmental pollution in the Gulf of Annaba (northeastern Algeria). The biomarkers selected were glutathione S-transferase (GST), acetylcholinesterase (AChE) and metallothioneins (MTs). Samples were collected...

Atmospheric particulate matter (PM) is a recognized risk factor contributing to a number of diseases in human populations and wildlife globally. Organic matter is a major component of PM, but its contribution to overall toxicity of PM has not been thoroughly evaluated yet. In the present work, the biological activity of organic extracts from PM1 (p...

Objective Year 2000 marked a turning point in stroke prevention and treatment in Portugal. In face of high incidence rates stroke awareness campaigns, close surveillance of vascular risk factors and implementation of hospital stroke units were advanced by the National Health Authorities. To understand the effect of such measures, we assessed change...

ICES - International Council for the Exploration of the Sea

A multidisciplinary approach was developed to estimate urban groundwater vulnerability to contamination combining hydrogeology, hydrogeochemistry, subterranean hydrogeotechnics, groundwater ecotoxicology and isotope tracers. Paranhos and Salgueiros spring waters in Porto City were used as a case study. Historical and current vulnerability scenarios...

Atmospheric deposition of particulate matter (PM) is recognized as a relevant input vector for toxic compounds, such as polycyclic aromatic hydrocarbons (PAHs), into the marine environment. In this work we aimed to analyse the biological activity and potential adverse effects of PM constituents to aquatic organisms. Organic extracts of atmospheric...

Recent findings indicate that different Endocrine Disrupting Chemicals (EDCs) interfere with lipid metabolic pathways in mammals and promote fat accumulation, a previously unknown site of action for these compounds. The antifoulant and environmental pollutant tributyltin (TBT), which causes imposex in gastropod snails, induces an "obesogenic" pheno...

Principal Component Analysis of the brain gene expression profile in TBT-exposed zebrafish. (PDF)

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TBT concentration in the water. Evaluation of the concentration of Tributyltin (TBT), Dibutyltin (DBT) and Monobutyltin (MBT) in the water from the 50 ng/L TBT (as Sn) exposure tanks at 20 days post fertilization. The aquaria were dosed twice daily (at 0 and 8 hours). (PDF)

Primer pair sequences used for RT-qPCR analysis. (PDF)

Principal Component Analysis of the liver gene expression profile in TBT-exposed zebrafish. (PDF)

Hepatic organotin levels. (PDF)

Visceral fat in TBT exposed zebrafish. (PDF)

Quantification of organotins in zebrafish liver. (PDF)

Zebrafish mortality. Mortality rate in zebrafish exposed to TBT (as Sn) at 60 days post fertilization (dpf). (PDF)

Shipping industry and seaborne trade have rapidly increased over the last fifty years, mainly due to the continuous increasing demand for chemicals and fuels. Consequently, despite current regulations, the occurrence of accidental spills poses an important risk. Hazardous and noxious substances (HNSs) have been raising major concern among environme...

The Water Framework Directive (WFD) provides an important legislative opportunity to promote and implement an integrated approach for the protection of inland surface waters, transitional waters, coastal waters and groundwaters. The transitional waters constitute a central piece as they are usually under high environmental pressure and by their inh...