Laura García-Vicente

• Doctor of Philosophy
• University of Salamanca

Glioblastoma (GBM) is an aggressive tumor with very bad prognosis. The urgent need to find new effective therapies is challenged by the unique characteristics of GBM, including high intra and intertumoral heterogeneity. Using single-nucleus transcriptomics (snRNA-seq), we characterized the panorama of a preclinical immunocompetent murine model base...

Background Glioblastoma is the most aggressive primary brain tumour and has a very poor prognosis. Inhibition of c-Src activity in glioblastoma stem cells (GSCs, responsible for glioblastoma lethality) and primary glioblastoma cells by the peptide TAT-Cx43266–283 reduces tumorigenicity, and boosts survival in preclinical models. Because c-Src can m...

Background: Malignant gliomas are the most frequent primary brain tumors and remain among the most incurable cancers. Although the role of the gap junction protein, connexin43, has been deeply investigated in malignant gliomas, no compounds have been reported with the ability to recapitulate the tumor suppressor properties of this protein in in vi...

Glioblastoma is the most aggressive primary brain cancer, with a median survival of 1 to 2 years. These tumours contain glioma stem cells (GSCs), which are highly tumorigenic, resistant to conventional therapies, and exhibit metabolic plasticity to adapt to challenging environments. GSCs can be specifically targeted by a short cell-penetrating pept...

Introduction Connexin43 (Cx43), the main gap junction channel-forming protein in astrocytes, is downregulated in malignant gliomas. These tumours are composed of a heterogeneous population of cells that include many with stem cell-like properties, called glioma stem cells (GSCs). Interestingly, restoring Cx43 in these cells reverses GSC phenotype a...

Here we present a protocol to study intracellular protein-protein interactions that is based on the widely used biotin-avidin pull-down system. The modification presented includes the combination of this technique with cell-penetrating sequences. We propose to design cell-penetrating baits that can be incubated with living cells instead of cell lys...