Laura Barrio Cano

• PhD
• Engineer at Institut Pasteur

During embryonic development, mutually antagonistic signaling cascades determine gonadal fate toward a testicular or ovarian identity. Errors in this process result in disorders of sex development (DSDs), characterized by discordance between chromosomal, gonadal, and anatomical sex. The absence of an appropriate, accessible in vitro system is a maj...

Streptococcus pneumoniae is a natural colonizer of the human respiratory tract and an opportunistic pathogen. Although epithelial cells are among the first to encounter pneumococci, the cellular processes and contribution of epithelial cells to the host response are poorly understood. Here, we show that a S. pneumoniae serotype 6B ST90 strain, whic...

The enteroinvasive bacterium Shigella flexneri forces its uptake into non-phagocytic host cells through the translocation of T3SS effectors that subvert the actin cytoskeleton. Here, we report de novo actin polymerization after cellular entry around the bacterium-containing vacuole (BCV) leading to the formation of a dynamic actin cocoon. This coco...

p38MAP kinase (MAPK) signal transduction pathways are important regulators of inflammation and the immune response; their involvement in immune cell development and function is still largely unknown. Here we analysed the role of the p38 MAPK isoforms p38γ and p38δ in B cell differentiation in bone marrow (BM) and spleen, using mice lacking p38γ and...

The enteroinvasive bacterium Shigella flexneri forces its uptake into non-phagocytic host cells through the translocation of T3SS effectors that subvert the actin cytoskeleton. Here, we report de novo actin polymerization after cellular entry around the bacterial containing vacuole (BCV) leading to the formation of a dynamic actin cocoon. This coco...

Streptococcus pneumoniae (Sp), a natural colonizer of the human respiratory tract, is a diverse species with over 90 serotypes. Initial pneumococcal colonization of the human nasopharynx induces two distinct host outcomes; asymptomatic carriage or symptomatic invasive pneumococcal disease depending on the serotype and the host response. Epithelial...

A current challenge in cell motility studies is to understand the molecular and physical mechanisms that govern chemokine receptor nanoscale organization at the cell membrane, and their influence on cell response. Using single-particle tracking and super-resolution microscopy, we found that the chemokine receptor CXCR4 forms basal nanoclusters in r...

NKX2 homeobox family proteins have a role in cancer development. Here we show that NKX2-3 is overexpressed in tumour cells from a subset of patients with marginal-zone lymphomas, but not with other B-cell malignancies. While Nkx2-3-deficient mice exhibit the absence of marginal-zone B cells, transgenic mice with expression of NKX2-3 in B cells show...

List of the differentially expressed genes in 18 months Em-NKX2-3 vs. wild-type using LIMMA (B>0, FDR<0.02; 630 genes) defining the Em-NKX2-3 transcriptional signature.

Supplementary Figures 1-7 and Supplementary Tables 1-9

Dynamics of WT and NKX2-3 transgenic B cells from 6 month-old mice. Caption: DIC and IRM images of SNARF-1-labelled WT B cells (red) and CFSE-labelled NKX2-3 transgenic B cells (green), mixed in 1:1 ratio, in contact with ICAM-1 membranes, over time (15 min; 3 frames/sec) are shown. Tracks are highlighted with dragon tail (red, WT B cells; green, N...

Dynamics of WT and NKX2-3 transgenic B cells from 12 month-old mice. DIC and IRM images of SNARF-1-labelled WT B cells (red) and CFSE-labelled NKX2-3 transgenic B cells (green), mixed in 1:1 ratio, in contact with ICAM-1 membranes, over time (15 min; 3 frames/sec) are shown. Tracks are highlighted with dragon tail (red, WT B cells; green, NKX2-3 tr...

Dynamics of WT and NKX2-3 transgenic B cells from 6 month-old mice in presence of CXCL12. DIC and IRM images of SNARF-1- labelled WT B cells (red) and CFSE-labelled NKX2-3 transgenic B cells (green), mixed in 1:1 ratio, in contact with ICAM-1 membranes coated with CXCL12, over time (15 min; 3 frames/sec) are shown. Tracks are highlighted with drago...

Dynamics of WT and NKX2-3 transgenic B cells from 12 month-old mice in presence of CXCL12. DIC and IRM images of SNARF-1- labelled WT B cells (red) and CFSE-labelled NKX2-3 transgenic B cells (green), mixed in 1:1 ratio, in contact with ICAM-1 membranes coated with CXCL12, over time (15 min; 3 frames/sec) are shown. Tracks are highlighted with drag...

Dynamics of WT and NKX2-3 transgenic B cells from 18 month-old mice. DIC and IRM images of SNARF-1-labelled WT B cells (red) and CFSE-labelled NKX2-3 transgenic B cells (green), mixed in 1:1 ratio, in contact with ICAM-1 membranes, over time (15 min; 3 frames/sec) are shown. Tracks are highlighted with dragon tail (red, WT B cells; green, NKX2-3 tr...

List of the differentially expressed genes in nine biopsies from SMZL patients vs. human CD19+ cells using LIMMA (B>0, FDR<0.03), defining the SMZL transcriptional signature.

Dynamics of WT and NKX2-3 transgenic B cells from 18 month-old mice in presence of CXCL12. DIC and IRM images of SNARF-1- labelled WT B cells (red) and CFSE-labelled NKX2-3 transgenic B cells (green), mixed in 1:1 ratio, in contact with ICAM-1 membranes coated with CXCL12, over time (15 min; 3 frames/sec) are shown. Tracks are highlighted with drag...

Background aims: Mesenchymal stromal cells hold special interest for cell-based therapy because of their tissue-regenerative and immunosuppressive abilities. B-cell involvement in chronic inflammatory and autoimmune pathologies makes them a desirable target for cell-based therapy. Mesenchymal stromal cells are able to regulate B-cell function; alt...

B cells use a plethora of TLR to recognize pathogen-derived ligands. These innate signals have an important function in the B cell adaptive immune response and modify their trafficking and tissue location. The direct role of TLR signaling on B cell dynamics nonetheless remains almost entirely unknown. In this study, we used a state-of-the-art two-d...

Lymphocytes use integrin-based platforms to move and adhere firmly to the surface of other cells. The molecular mechanisms governing lymphocyte adhesion dynamics are however poorly understood. In this study, we show that in mouse B lymphocytes, the actin binding protein vinculin localizes to the ring-shaped integrin-rich domain of the immune synaps...

Continuous migration of B cells at the follicle contrasts with their stable arrest after encounter with antigen. Two main ligand/receptor pairs are involved in these cell behaviors: the chemokine CXCL13/chemokine receptor CXCR5 and antigen/BCR. Little is known regarding the interplay between CXCR5 and BCR signaling in the modulation of B-cell dynam...