Latiful Bari

University of Dhaka · Center for Advanced Research in Sciences

The prevalence of undesirable microbial contaminants in fresh vegetables, often consumed raw as salad vegetables, is a concern for the global public health community. In this study, samples from different local markets in Dhaka were collected to evaluate the microbiological quality and safety of four raw salad vegetables, namely, lettuce, tomato, c...

In order to assess the activity of the live yeast, this study suggested measuring the menadione-mediated current in real-time following the addition of accelerators or inhibitors to the suspension of yeast cells. The study attempted to demonstrate the association between menadione reduction and proliferation of yeast by employing carbohydrates inci...

Aloe Vera juice is an integral part of the food culture in Bangladesh as this type of street juice is usually prepared by instant processing before consumption. This juice is widely accepted by many people in developing countries because they are viewed as nutritious, inexpensive, convenient, and attractive. This research presents a comprehensive a...

The wet market poultry processing is a concern of spreading the microbial pathogens into the environment or to the products. This study evaluated microbiological contamination in wet market poultry slaughter and processing lines, as well as the efficacy of non-chlorine sanitizers for improved sanitation. Swab samples from inner drum surfaces, de-fe...

Roselle (Hibiscus sabdariffa L.) flower is a commonly consumed vegetable in Bangladesh and have culinary value to preparation of soup, jelly, and baking. Since previous studies suggested the presence of anthocyanins in roselle calyces, this study was designed to enrich anthocyanins from the calyces of this flower. This study explored that addition...

This study investigates the effects of heat and ultrasonic treatments on the physicochemical parameters and rennet-induced coagulation properties of milk from a variety of species, including cow, goat, buffalo, and donkey. Milk samples were subjected to heat treatments at different temperatures (65 °C, 80 °C, 90 °C, 100 °C) and ultrasonic treatment...

Roselle (Hibiscus sabdariffa L.) flower is a commonly consumed vegetable in Bangladesh and have culinary value to preparation of soup, jelly, and baking. Since previous studies suggested the presence of anthocyanins in roselle calyces, this study was designed to enrich anthocyanins from the calyces of this flower. This study explored that addition...

The wet market poultry processing is a concern of spreading microbial pathogens into the environment or to the products. So, this study evaluated the microbiological contamination in poultry settings, i.e., slaughter and processing lines of wet market poultry shops, and the use of nonchlorine sanitizer to improve the sanitation. Swab samples from i...

The safety of vegetable food is compromised by various factors, including the inefficient or excessive use of sanitizers. Instances of individuals falling ill after consuming raw vegetables have been reported, with outbreaks of diseases caused by pathogens on fresh vegetables becoming increasingly prevalent globally, attracting significant media co...

The safety of vegetable food is compromised by various factors, including the inefficient or excessive use of sanitizers. Instances of individuals falling ill after consuming raw vegetables have been reported, with outbreaks of diseases caused by pathogens on fresh vegetables becoming increasingly prevalent globally, attracting significant media co...

This study aimed to investigate antibiotic residues such as oxytetracycline, ciprofloxacin, enrofloxacin and levofloxacin, in both pasteurised and raw cow's milk. A method using high-performance liquid chromatography with a UV detector (HPLC-UV) was developed and validated following International Conference on Harmonization (ICH) guidelines for sim...

Legionella cause Legionnaires’ disease and Pontiac fever, and, more rarely, extrapulmonary infections, collectively referred to as legionellosis. Legionella pneumophila causes serious pneumonic infections, and water and Air Conditioning systems are a common source. We performed this study focusing on identifying and assessing the potential sources...

Degradation of soil health is a growing concern as both human health and soil microbial community are directly related to this. One of the main hazards to soil deterioration in Bangladesh is soil salinization. In this study, the microbiological status of two commercial biofertilizers, an agricultural soil, and saline soil in Bangladesh were analyze...

Rapid urbanization and poor hygiene knowledge among city dwellers have led to a growing sanitation crisis in urban areas of Bangladesh and potential exposure to fecal contamination in the urban water systems due to inadequate sanitation and poor fecal sludge management. This study was carried out from April to June 2022 to assess the fecal indic...

This study presents a newly developed chitosan-collagen material composite with great potential for dye removal. With a remarkable dye separation efficiency of 88.23%, this composite proves highly effective in eliminating dyes from solutions, offering significant promise for wastewater treatment and environmental remediation. Operating optimally un...

Various reasons, including use, abuse, and excess using sanitizers ineffectively or at low doses, endanger vegetable food safety. People have become ill after eating raw vegetables, and pathogen-caused disease outbreaks on fresh vegetables have become more common worldwide, are widely covered in the media, and affect the profitability of producing...

The usage of biofuel as a potential alternative source for fossil fuels has gained significant importance in the last two decades. Bio-ethanol, an ideal bio-fuel could be used to reduce the total contribution of greenhouse gas from the atmosphere. Agricultural byproducts can be used for the production of bioethanol. In this study, rice bran and soy...

The presence of trace metals in aquatic ecosystems can have detrimental effects on fish survival. The Tanguar haor, a Ramsar conservation wetland, receives sediment and water from multiple transboundary rivers. However, there have been limited studies on the metal concentrations in fish species in this sediment-rich wetland. This study aimed to ana...

Simple and low-cost postharvest technologies designed for smallholders are usually less effective than the recommended but capital-intensive mechanical refrigeration system. Thus, to improve the efficacy of these technologies, it could be beneficial to use them in an integrated system. This study combined the use of non-chlorine sanitizer and modif...

Background Abiotic stressors impair crop yields and growth potential. Despite recent developments, no comprehensive literature review on crop abiotic stress assessment employing deep learning exists. Unlike conventional approaches, deep learning-based computer vision techniques can be employed in farming to offer a non-evasive and practical alterna...

Traditionally, inorganic chemical-based fertilizers is used for soil management strategies, which can cause public health and environmental threats. Alternatively, bio-fertilizer can be used to increase the productivity and sustainability of soil without causing environmental pollution. The present study aimed to cost-effectively produce bio-fertil...

Technology for providing clean drinking water to remote areas of low-resource nations remains a significant challenge for human life The study aimed to develop a simple technology for rural households that might be adopted to utilize the bactericidal properties of metals. Three thick metal sheets made of copper, zinc, and brass (an alloy of copper...

More than 4.5 million people nationwide have experienced diarrhea in the country’s most recent outbreak, which began on March, 2022. About 70% to 80% of patients (of whom 23% had severe diarrhea or cholera) were hospitalized. Several factors were associated with the risk for diarrhea include; unhygienic and unsafe environment, age, low educational...

Background: The human pathogen Legionella pneumophila causes a serious pneumonia-like respiratory disease called Legionnaires' disease, mainly in elderly and immunocompromised individuals. This pathogen can be found in the water distribution systems of large constructions with cooling towers which is a common phenomenon at present in Bangladesh due...

Fecal Contamination in Multiple Water Samples

Planar (2D) nanomaterials are garnering broad recognition in diverse scientific areas because of their intrinsic features. Herein, bulk graphitic carbon nitride (g-C3N4) was prepared from melamine, which was exfoliated to produce g-C3N4 nanosheets. The prepared g-C3N4 nanosheets were characterized by transmission electron microscopy (TEM), atomic f...

In conventional preservation of hides and skins, the use of NaCl (50%) evolves enormous salinity and total dissolved solids (TDS) in soaking effluent during leather processing. Plant-based skins preservation is a sustainable solution in reducing salinity and TDS from leather industry to replace conventional curing. In this study, the application of...

Introduction: Poor hygiene practices and restaurant food handling put consumers at risk of enteric disease, especially in developing countries. This study aims to assess some cheap and crowded restaurants' hygiene and food handling practices in Dhaka city of Bangladesh Method: Total 93 samples were collected from various cheap and crowded restauran...

The term “stress” refers to any extracellular influence that threatens the ability of microorganisms to perform their living functions. In nature, microorganisms are constantly exposed to diverse changes in temperature, oxygen, moisture, light, pH, and chemical composition. Thanks to their wide array of molecular responses that make them survive by...

Mining water quality must be monitored regularly to assess the potential impact on local water resources, surface water, and groundwater. This report determined the physicochemical, heavy metals content, and microbiological characteristics of mining drainage water and paddy field water in the surrounding area to determine the extent of pollution. T...

Raw salad vegetables are evaluated for the consumer’s perceptions on taking ready to eat fresh cut-vegetables and the effectiveness of some non-chlorine disinfectants [peracetic acid (PAA), shell powder (SP) and hydrogen peroxide (H2O2)] in improving the microbial safety, quality and shelf life of ready to eat fresh-cut vegetables (lettuce, carrot...

Considering the potential health hazardous effects, the distribution and deposition of heavy metals and trace elements including Mn, Fe, Cu, Zn, Cd, Cr, and Pb in different tissues (skin, brain, liver, meat, and bone) of broiler and layer chickens, their feeds, litter, and eggs were analyzed using atomic absorption spectrophotometer (AAS) after nit...

Considering the potential health hazardous effects, the distribution and deposition of heavy metals and trace elements including Mn, Fe, Cu, Zn, Cd, Cr, and Pb in different tissues (skin, brain, liver, meat, and bone) of broiler and layer chickens, their feeds, litter, and eggs were analyzed using atomic absorption spectrophotometer (AAS) after nit...

Bangladesh experiences a second wave of COVID-19 since March 2021, despite the nationwide vaccination drive with the ChAdOx1 (Oxford-AstraZeneca) vaccine from early February 2021. Here, we characterized 19 nasopharyngeal swab (NPS) samples from COVID-19 suspect patients using genomic and metagenomic approaches. Screening for SARS-CoV-2 by RT-PCR an...

The emergence of antibiotic resistant bacteria is a major health concern worldwide in recent years. The objective of this study is to establish the larvae of the silk moth (commonly known as silkworm), Bombyx mori as an infection model to study antibacterial effect of antibiotics against Klebsiella pneumoniae. In this study, the pathogenicity of a...

Molicular diagnosis, if culture-free, is always considered as the relatively safe diagnostic tool, as a confirmatory diagnosis is possible from a minimum number of microorganisms. However, processing the specimens still requires adequate biosafety and biosecurity measures depending on the risk group of the target pathogens or pathogens supposedly b...

The present study was conducted to assess the presence of bacterial populations at the upper sub-surface water from the offshore areas near the South East Coast of Bangladesh, the northern Bay of Bengal and to evaluate their antibiotic resistance pattern. Water samples were collected from 5 stations near the South East Coast of Bangladesh, the nort...

The presence of foodborne pathogen in betel leaf represents a major threat from the public health perspective and is an important export obstacle for South East Asian countries causing huge economical loss to the betel leaf farmers. Hence, to comply with national and international food safety requirements, use of non-chlorine sanitizer and low-cost...

Chronic Suppurative Otitis Media (CSOM) is characterized by persistent otorrhoea from the middle ear through a perforated tympanic membrane. It is regarded as one of the most important causes of hearing impairment in developing countries. In many cases, treating CSOM with antibiotics generates fears of resistance. Unlike conventional antibiotics, h...

Morbidity and mortality resulting from the consumption of microbial contaminated foods remain significant problems in modern society, and the ability to conduct epidemiological investigations and intervene in foodborne illnesses is a critical part of the existing public health infrastructure. Ensuring safe food supply is a major challenge to the fo...

A pure bacterial culture remains essential for the study of its virulence, antibiotic susceptibility, and genome sequence in order to facilitate the understanding and treatment of diseases caused by it. Culture media is of fundamental importance for most microbiological tests, to obtain pure cultures, to grow and count microbial cells, and to culti...

This study was designed to evaluate the use of non‐chlorine sanitizer in enhancing quality, microbial safety, and increased shelf life of carrots under various low‐cost packaging and storage at commercial cold storage. The study results revealed that irrespective of packaging conditions, none of the washed carrots stored in a commercial cold storag...

In this study, we estimate the proximate compositions, phytochemicals (po-lyphenol, flavonoids, flavonol, tannin, protein, carbohydrate, reducing sugar, and β carotene), antioxidant activities, vitamins, minerals, and heavy metals of the four pulses (mung, anchor, chickpea, lentils) and compare among them to find out more nutritious pulse samples....

Cytotoxic effects of menadione on normal and cytochrome c-deficient yeast cells were examined on the basis of the cell growth rate, NAD(P)H concentration, reactive oxygen production, plasma membrane H+-ATPase activity, and ethanol production. In aerobically or anaerobically cultured yeast cells, NAD(P)H concentration decreased with increasing conce...

The increasing clinical incidence of antibiotic resistance in bacteria is a major global health care issue. Rampant use of antimicrobials is one of the major reasons of the dramatic rise in antibiotic-resistant bacterial strains. Suitable animal models are required to improve our understanding of bacterial pathogenicity, evolution and search for no...

Introduction Many emerging and reemerging pathogens have been identified as major public health threats in Bangladesh. Collection, transportation, and storage of infectious materials and management of generated waste from diagnosing those diseases require strict adherence to biosafety and biosecurity practices. Such activities in Bangladesh need su...

This study demonstrated that glucose-induced proton release from yeast cells was more sensitive to various inhibitors than cell proliferation. In this study the inhibition of glucose-induced proton release was determined on the basis of color change of pH indicator, methyl red, from pH 5 to pH6 at cell density of 2.5 × 10⁷ cells/ml. When yeast cell...

In this study, we evaluated the ability of waste shell powder (WSP) and moringa seed powder (MSP) individually or in combination to eliminate dye, heavy metal and resistant bacteria from the industrial wastewater (IWW). The presence of dyes, heavy metals, approximately 7.0 log CFU/ml of aerobic bacteria and 3.0-4.0 log CFU/ml of other pathogens inc...

Rhizobia are Gram-negative soil-inhabiting bacteria commonly found in nodules of leguminous plants. These bacteria exclusively fix atmospheric nitrogen into ammonia, which is convertible to urea to serve natural fertilizer to the plants. However, rhizobial microbiota of Bangladesh have been studied poorly. Here, twenty isolates were identified as R...

The national food system of Bangladesh has made substantial progress since experiencing famine in 1974, soon after independence. After the famine, the government placed a strong emphasis on policies required to attain grain self-sufficiency; since attaining self-sufficiency, the production system, policies related to it, and resulting diets have be...

The application of large quantity of foreign microbial inoculants (as biofertilizer) may create selective pressure on the existing soil microorganisms or its funa and flora, In addition, foreign microbial inoculants, may act as a predator, or can compete or suppress other beneficial microorganisms exist in soil, or may not be able to survive/adapt...

This chapter discusses Yersinia enterocolitica, a zoonotic pathogen that causes yersiniosis in humans and animals. Yersinia enterocolitica is a Gram‐negative, non‐spore‐forming, non‐toxin‐producing, rod‐shaped bacterium. It is an aerobic bacterium but can grow anaerobically and is a good competitor with other bacteria. An important property of this...

Chronic Suppurative Otitis Media (CSOM) is a common infectious disease of middle ear; characterized by a persistent discharge through a perforated tympanic membrane. CSOM is one of the prime causes of hearing impairment in developing countries. Indiscriminate use of antibiotics for treating CSOM promotes antibiotic resistance leading to accelerated...

This study was conducted to evaluate the effectiveness of a simplified, low cost, pasteurization device in inactivating the diarrheal pathogens present in pond/lake/river water in order to provide safe potable water to people living in the rural areas of low resource countries. In this process, water in polyethylene bags was exposed to sunshine, wh...

The presence of undesirable heavy metals, pesticide residues, and microbial contaminants in fresh produces is a worldwide public health concern. This study was undertaken to evaluate the residual pesticides (Diazinon, Malathion, Cypermethrin, Dimethoate, Quinalphos, and Chloropyrofos), heavy metal contamination (Pb, Cd, and Cr), and microbiological...

Background. A simple, rapid, accurate, precise, and economic spectrophotometric methods for estimation of vitamin D in from food, feed, pharmaceutical and environmental samples have been developed. Method. Vitamin D has absorbance maximums at 265.0 nm, so absorbance was measured at the same wave length for the estimation of vitamin D. Absorbance is...

The excessive use of chemical preservatives is a serious problem worldwide, which necessitates the discovery of new classes of antimicrobials from other sources like herbs and spices for preservation of food and/or food products. In this study, antibacterial activity of essential oils (EOs) such as cinnamaldehyde and carvacrol were tested against f...

Chemical preservatives for food preservation at high concentration are hazardous for public health. The natural antimicrobials from plants, herbs and spices have been given priority as alternatives of the chemical preservatives. In this study, antibacterial activity of the essential oil (3%) from black cumin seeds was tested against five food borne...