Latif M. A.

• PhD
• Chief Scientific Officer at Bangladesh Rice Research Institute

The rice blast caused by Magnaporthe oryzae is one of the major and recurrent threats to sustainable rice production in Bangladesh. To mitigate this problem, the present study was undertaken with the aim to investigate the inhibitory effects of commercially available silver nanoparticles (Ag-NPs) on the growth and development of Magnaporthe oryzae,...

This study presents a comprehensive investigation into the characterization and management of seedling blight pathogens that affect tray-based seedling production, which is a significant limiting factor for mechanized cultivation in Bangladesh, particularly during the Boro season (December-June). Various types of seedling blight pathogens were isol...

Landraces serve as important reservoirs and primary sources of disease resistance genes. Harnessing these genes in advanced breeding lines is crucial to combat emerging pathogenic races and develop disease-resistant crop varieties. The International Rice Research Institute (IRRI) has already sequenced the rice genome, including 186 germplasm from B...

Rice plant suffer from different biotic and abiotic stresses, among the biotic stresses diseases are major threat for rice production. Disease management mainly dependent on application of chemicals,but chemicals are hazardous for both human health and environment. Application of nano-particles in rice disease management would be an alternative to...

An experiment was conducted in the laboratory and field to study the interaction among three sheath diseases of rice namely sheath blight, sheath spot and aggregated sheath spot and their causal agents, Rhizoctonia solani, R. oryzae and R. oryzae-sativae. In addition to RFLP-PCR analysis and DNA sequencing of three sheath disease species, antagonis...

Bacterial blight (BB) disease, caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the major bottlenecks of rice (Oryza sativa) production. The present study was undertaken to identify BB-resistant parental lines of hybrid rice based on pathogenicity tests and molecular screening. Three virulent races of Xoo, i.e. BXo93, BXo822 and BXo887 were...

Bacterial blight, one of the oldest and most severe diseases of rice poses a major threat to global rice production and food security. Thereafter, sustainable management of this disease has given paramount importance globally. In the current study, we explored 792 landraces to evaluate their disease reaction status against three highly virulent str...

Rice blast disease is one of the major bottlenecks of rice production in the world including Bangladesh. To develop blast resistant lines, a cross was made between a high yielding but blast susceptible variety MR263 and a blast resistant variety Pongsu seribu 2. Marker-assisted backcross breeding was followed to develop F1, BC1F1, BC2F1, BC2F2, BC2...

Bacterial blight disease (causal organism: Xanthomonas oryzae pv. oryzae) is an economically significant menace to rice cultivation in Bangladesh as well as in the world, which reduces significant yield loss in rice and hampers food security. The most sustainable strategy to fight this disease is the adoption of disease-resistant cultivars. The mor...

Rice is an important cereal worldwide and it occupies the top position among the cereals in Bangladesh. Rice plant suffers from around 32 diseases of which ten are major in Bangladesh at present. Among the diseases, Bacterial Blight (BB) caused by X. oryzae pv. oryzae (Xoo) considered as a most destructive disease occurs in both rainfed and irrigat...

Bacterial blight, one of the oldest and severe diseases of rice poses a major threat towards global rice production and food security. Thereafter, sustainable management of this disease has given paramount importance globally. In the current study, we explored 792 landraces to evaluate their disease reaction status against three highly virulent str...

Farmers’ steps in the seedbed to prevent rice tungro disease: Tungro disease occurs in Cumilla region in Aus, Aman and Boro seasons. However, it also takes place in Chattogram, Feni, Noakhali, Chandpur, Brahmanbaria, Barishal, Sylhet, Dhaka, Mymensngh, Gazipur and 3 rice cropped regions in Bangladesh. Every year, hundreds hectare of rice fields are...

Farmers’ steps to manage blast disease of rice: Rice blast disease is one of the devastating disease of rice. This is caused by one of the fungal organism Pyricularia grisea. This disease occurs mainly in Aman and Boro season in Bangladesh. Incidence and severity of blast disease of rice is recorded in all agro-ecological zones (AEZs) of Bangladesh...

Purpose Top management commitment (TMC) and prosocial voice behaviour in Lean teams are vital for the successful Lean implementation. This study aims to investigate how TMC influences Lean team members’ prosocial voice behaviour and how such changed voice behaviour affects the outcome of Lean implementations. Design/methodology/approach The author...

Fusarium moniliforme, a causal agent of rice Bakanae is a seedborne fungal disease which infects plants through the roots or crowns and it then grows systemically within the plant. From the in-vitro and in-vivo results, it was concluded that Bavistin, Knowin, Haydazim, Topsil plus and Sunphanate @ 0.3% could be effective as seed treating fungicides...

Bacterial blight (BB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is a serious threat to rice production in the world. Identification of Xoo races pattern, distribution and deployment of race‐specific resistant genes is a sustainable strategy to develop bacterial blight resistant varieties. In this study, a total of 118 isolates of Xoo were colle...

Rice Tungro disease caused by Rice Tungro Virus (RTV) transmitted by vector Green Leaf Hopper (GLH), Nephotettix virescens. Every year tungro disease infection is devastating in many rice growing areas in Cumilla region as well as countrywide. In Bangladesh, yield loss due to tungro was reported to be as high as 100% under severe conditions. Three...

A study was conducted to assess the resistant sources, genetic variability and phylogenetic relationship among 44 rice genotypes based on pathogenicity, genetic parameters of morphological traits and simple sequence repeat markers. Results demonstrated that, 9 genotypes were resistant, 9 were moderately resistant and the rest of the genotypes were...

PATHOGENICITY OF BLAST ISOLATES AND GENETIC VARIATION OF RESISTANCE OF RICE IN BANGLADESH ABSTRACT Blast caused by Pyricularia oryzae Cavara is one of the most destructive diseases and recurrent problem in Bangladesh rice (Oryza sativa L.). Most of the high yielding, fine and aromatic varieties are severely affected by blast and causes serious yiel...

Eight promising hybrids along with three checks were evaluated for yield and yield contributing traits to observe their genetic variability, heritability, correlation and path coefficient analysis during T. Aman season 2014. The results indicated that the highest genotypic variance was recorded in spikelet panicle-1 followed by effective tiller m-2...

Tungro disease of rice caused by Rice Tungro Virus (RTV). It is transmitted by vector Green Leaf Hopper (GLH), Nephotettix virescens. Tungro disease is devastating in Cumilla region as well as countrywide. In Bangladesh, yield loss due to tungro was reported to be as high as 100% under severe conditions (BRRI 2019-20). Three crop rice (Rice-Rice-Ri...

Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae is one of the major hindrances in rice (Oryza sativa L.) production across the world including Malaysia. Therefore, the development of disease-resistant varieties remains a very economical and effective method of controlling BLB in rice. Based on this background, this study was con...

Rice (Oryza sativa L.) is the main food for the largest community in the world, and its demand is increasing day by day. It is a highly palatable energy-rich food as a result of its starch content. Among different parts of rice, rice bran consists of high protein and energy. Brown rice is the most nutritious and consists of different types of fat (...

Grain and nutritional qualities in rice are important attributes that rice breeders have dedicated to improve and tailor according to the preference of consumers. The recent advancement of DNA marker and sequencing technologies, mutation discovery and gene editing technologies has revolutionized the breeding approaches for developing rice with impr...

Aggregate sheath spot disease of rice caused by Rhizoctonia oryzaesativae has emerged in higher incidence in North-Western region of Bangladesh. Thirty isolates of R. oryzae-sativae were studied by using morphological and molecular marker. Isolates were confirmed using specific primer where a single band of 1200bp was amplified. Two distinct groups...

Field experiment was carried out in hot and humid summer (Transplanted Aus) season to realize the yield loss of a susceptible rice variety Purbachi inoculated with bacterial blight (BB). Treatments consist of BB inoculations at different crop growth stages like maximum tillering (MT), panicle initiation (PI), booting (Bt), flowering and heading sta...

Eight promising hybrids along with three checks were evaluated for yield and yield contributing traits to observe their genetic variability, heritability, correlation and path coefficient analysis during T. Aman season 2014. The results indicated that the highest genotypic variance was recorded in spikelet panicle-1 followed by effective tiller m-2...

Identification of physiological races of Xanthomonas oryzae pv oryzae, evaluation of bacterial blight resistant pyramid lines and development of resistant varieties through marker assisted selection in Bangladesh: Experiment was conducted in Plant Pathology Division, BRRI Gazipur funded by STRASA, IRRI. Total 102 rice materials were tested against...

GR2-E BRRI dhan29 Golden Rice was developed and selected at IRRI from cross between GR2-E Kaybonnet Golden Rice and BRRI dhan29.TheKaybonnetwas modified through agrobacterium-mediated transformation of beta carotene gene (ZmPSY1) from maize using plasmid pSYN12424.Event GR2E was uniquely identified using a multiplex PCR method employing three prime...

GR2-E BRRI dhan29 Golden Rice was developed and selected at IRRI from cross between GR2-E Kaybonnet Golden Rice and BRRI dhan29.TheKaybonnetwas modified through agrobacterium-mediated transformation of beta carotene gene (ZmPSY1) from maize using plasmid pSYN12424.Event GR2E was uniquely identified using a multiplex PCR method employing three prime...

Rice blast disease, caused by Magnaporthe oryzae, is the most important and most devastating rice disease globally. For genetic analysis of resistance to rice blast, the present study used a BC2F1 population developed through marker-assisted backcrossing from a cross between blast susceptible MR263 and blast resistant Pongsu Seribu 1 (PS1). We sele...

Aromatic rice cultivars constitute a small but special group of rice and are considered the best in terms of quality and aroma. Aroma is one of the most significant quality traits of rice, and variety with aroma has a higher price in the market. This research was carried out to study the genetic diversity among the 50 aromatic rice accessions from...

All the 147 SSR markers that were used in this study are presented in a supplementary table.

Genetic variation in blast resistance was clarified in 334 Bangladesh rice accessions from 4 major ecotypes (Aus, Aman, Boro and Jhum). Cluster analysis of polymorphism data of 74 SSR markers separated these accessions into cluster I (corresponding to the Japonica Group) and cluster II (corresponding to the Indica Group). Cluster II accessions were...

Arsenic (As) toxicity has become a major problem since a few decades in different parts of Bangladesh mainly due to the massive groundwater extraction for both drinking and irrigation purposes. This study was undertaken to investigate the spatial variation and spatial dependency of soil As at four locations in Bangladesh. Composite soil samples wer...

IntroductionThe brown planthopper (BPH), Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), is a notorious insect pest of rice. Nutrient supplementation can alter plant biochemical compositions which may indirectly affect on ecological fitness parameters of its insect pest. However, few findings are available on nutrient-induced ecological fitness...

Screening of rice genotypes (NILs and pyramid lines) against bacterial blight disease of rice in Bangladesh: rice genotypes (NILs and pyramid lines) were evaluated against bacterial blight (BB) disease of rice due to find out the resistant gene(s) against bacterial blight pathogen in Bangladesh environment. The experiment was conducted under artifi...

Nowadays, in extreme changing environments, development of submergence tolerance variety is necessary for ensuring crop production stability where, it is known that Malaysian commercial rice varieties such as MR219, MR220 and MR263 were severely susceptible to submergence. First step towards the development of submergence tolerance variety starts w...

Bacterial blight disease caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most devastating biotic stresses of rice in all rice growing countries of the world. Find out the resistant source is the first step for the development of resistant rice variety through breeding techniques. In this experiment, one hundred land races including susc...

Fragrance in rice is an appealing attribute to consumers. The increasing demand for fragrant rice highlights the need to develop fragrant rice variety that suit the preference of local consumers in addition to reduce fragrant rice imports. Marker-assisted backcrossing (MABC) was employed to develop advanced fragrant rice lines from the cross betwee...

Brown planthopper (BPH), Nilaparvata lugens Stål (Hemiptera: Delphacide), is a destructive insect pest of rice, Oryza sativa L. (Poaceae), in rice-producing areas worldwide. Host plant resistance is a major aspect of managing this pest. In this study, a mapping population consisting of 150 F3 lines, derived from a cross of MR276 and Rathu Heenati,...

Extraction of RNA of high quality and integrity is essential for gene expression studies and all downstream RNA-based techniques. The leaves of 16 merit Malaysian rice varieties were used to isolate total RNA using five different methods. The quantity, quality and integrity of extracted RNA were confirmed using three different means. The ratios of...

Background: The rice cultivar MR219 is famous for its better yield and long and fine grain quality; however, it is susceptible to blast disease. The main objective of this study was to introgress blast resistance genes into MR219 through marker-assisted selection (MAS). The rice cultivar MR219 was used as the recurrent parent, and Pongsu Seribu 1...

Arsenic pollution became a great problem in the recent past in different countries including Bangladesh. The microlevel studies were conducted to see the spatial variation of arsenic in soils and plant parts contaminated through ground water irrigation. The study was performed in shallow tube well command areas in Sadar Upazila (subdistrict), Farid...

Full Text HTML | PDF Print | PDF with Links | The pathogenicity of 331 blast isolates (Pyricularia oryzae Cavara) collected from different regions and ecosystems for rice cultivation in Bangladesh was evaluated by compatibility on 23 differential varieties (DV), each harboring a single blast resistance gene, and susceptible 'Lijiangxintuanheigu' (L...

A cross was made between MR219 (high yielding but submergence intolerant) and Swarna-Sub1 (submergence tolerant) to produce submergence-tolerant rice variety using the marker-assisted backcrossing (MABC) method to protect the farmers of low-lying land from flash floods during rain. Knowledge of yield and yield contributing factors plays a vital rol...

Assessment of genetic diversity and molecular characterization among elite rice cultivars of Bangladesh is important for varietal identification. Genetic diversity of 20 geographical indication (GI) rice landraces of Bangladesh was analyzed for 30 loci using simple sequence repeat markers to characterize the varieties and also to establish the sove...

The leaf spot or Tikka disease (Cercospora arachidicola and Cercosporidium personatum) of groundnut (Arachis hypogea L.) is a predominant, devastating and economically important foliar fungal disease and a major yield reducing factor of groundnut, not only in Bangladesh, but also all over the world. The yield loss was calculated in the groundnut va...

The experiments were carried out during 2010 to 2011 to find out the time of application and level of inoculum of T. harzianum for controlling root rot (F. solani) and collar rot (S. rolfsii) of lentil (Lens culinaris Medik). F. solani and S. rolfsii and their antagonist, Trichoderma were collected from different pulses growing areas of Bangladesh....

Eighteen isolates of Rhizoctonia solani collected from infected rice plants in four different locations of Bangladesh were studied by using morphological characters and molecular markers. Anastomosis study with a reference isolate confirmed that all the isolates belonged to R. solani. Significant variation was observed in sclerotial size, shape and...

p>Cross infection between rice and wheat blast fungi was investigated in a series of experiments conducted under controlled glasshouse condition following a completely randomized design. Two rice (BRRI dhan29 and LTH) and two wheat (BARI Gom25 and BARI Gom26) varieties were grown in plastic trays as sole and rice-wheat mixed crop culture. Plants we...

To examine the impact of regional and seasonal variations on the incidence and severity of sheath rot, a major seed-borne disease of rice caused by Sarocladium oryzae, data on incidence and severity were collected from 27 selected fields in the Gazipur, Rangpur, Bogra, Chittagong, Comilla, Gopalgonj, Jessore, Manikgonj, and Bhola districts of Bangl...

Blast is the most common biotic stress leading to the reduction of rice yield in many rice-growing areas of the world, including Malaysia. Improvement of blast resistance of rice varieties cultivated in blast endemic areas is one of the most important objectives of rice breeding programs. In this study, the marker-assisted backcrossing strategy was...

Disease susceptibility and genetic variability in 10 eggplant genotypes were studied after inoculating Phomopsis vexans under confined field conditions. Random amplified polymorphic DNA (RAPD) markers were used to assess genetic variation and relationships among eggplant genotypes. The disease index of leaves ranged 0.208-13.79%, while fruit infect...

An experiment was carried out at the Pulse Research Sub-Station (PRSS), Madaripur, Bangladesh during three consecutive years of 2008-2009, 2009-2010 and 2010-2011 to screen out the suitable high yielding chickpea varieties that are resistant to Botrytis Gray Mold (BGM) under artificial inoculated condition. Twenty four deshi chickpea lines/varietie...

After yield, quality is one of the most important aspects of rice breeding. Preference for rice quality varies among cultures and regions; therefore, rice breeders have to tailor the quality according to the preferences of local consumers. Rice quality assessment requires routine chemical analysis procedures. The advancement of molecular marker tec...

The study was conducted to identify the suitable SSR markers in relation to blast disease of rice. Backcross breeding was applied and BC2F1 generation was produced using rice variety Pongsu Seribu 2 as blast resistant parent and MR219 as susceptible parent. Eleven SSR markers related to blast resistance genes (Pi gene) were found highly polymorphic...

The study was carried out in the University Putra Malaysia (UPM) Rice Research Centre to evaluate the yield performance of newly developed selected blast resistant plants of BC2F3 generations derived from a cross between MR263, a high yielding rice variety but blast susceptible and Pongsu Seribu 1, donor with blast resistant (Pi-7(t) and Pi-d (t)1,...

Recurrent parent genome (RPG) recovery is the most important step of marker assisted backcross breeding. This breeding approach is used to develop new varieties by inserting a particular gene of interest into the background of a modern variety, and removing undesirable segments linked with the target allele, which can survive after many generations...

Developing cultivars resistant against different biotypes of brown planthopper (BPH) through marker-assisted selection (MAS) is an effective management strategy to control this pest. In this study, 28 polymorphic simple sequence repeat (SSR) markers were analyzed in 108 F3 progenies derived from the cross of Rathu Heenati and MR276 rice cultivars t...

Segregation distortion analysis in plant breeding is highly important because deviation factors are occasionally serious barriers for introgression of genes of interest into cultivated species. Also, the identification of major QTLs by high linkage disequilibrium is highly useful for breeders in order for crop improvement programs and the further f...

When a phenotype of interest is associated with an external/internal covariate, covariate inclusion in quantitative trait loci (QTL) analyses can diminish residual variation and subsequently enhance the ability of QTL detection. In the in vitro synthesis of 2-acetyl-1-pyrroline (2AP), the main fragrance compound in rice, the thermal processing duri...

Rice false smut (RFSm) has lately been recognized as an emerging disease worldwide. Its alarming prevalence in Bangladesh in the transplant Aman rice in the last three years has been widely reported. However, there is no effective control measure against the disease in this country. We hypothesized that manipulation of flowering time in aman rice m...

The rice blast fungus Magnaporthe oryzae is a serious pathogen that jeopardises the world's most important food-security crop. Ten common Malaysian rice varieties were examined for their morphological, physiological and genomic responses to this rice blast pathogen. qPCR quantification was used to assess the growth of the pathogen population in res...

Abstract: Pattern of disease spread provides improved knowledge on how the pathogen introduces itself and interacts with environment in fields and expresses as a disease. It is especially significant when epidemiology of a disease, such as Rice False Smut (RFSm), is unclearly understood. Not reported before, this study attempted an analysis of spat...

BACKGROUND Genetic analysis using generation mean analysis is a tool for designing the most appropriate breeding approaches to developing varieties of rice. It estimates the gene actions that control quantitative traits, as well as the additive, dominance and epistatic effects. This study was conducted using three rice populations that were derived...

Genetic variability with mutagenic agents has been employed in plant breeding due to its significant contribution to the improvement of the existing rice genotypes by using genetically diverse parents. This study evaluated the genetic variability and diversity of mutant rice using quantitative traits and inter-simple sequence repeats (ISSR) molecul...

Background: Blast caused by the fungus Magnaporthe oryzae is a significant disease threat to rice across the world and is especially prevalent in Malaysia. An elite, early-maturing, high-yielding Malaysian rice variety, MR263, is susceptible to blast and was used as the recurrent parent in this study. To improve MR263 disease resistance, the Pongs...

Experiments were carried out to study the mechanisms for heat tolerance in chili pepper (Capsicum annuum). To assess these mechanisms, six genotypes were evaluated for cellular membrane thermostability (CMT) and for HSP70 gene expression. The plants were grown in an experimental plant growth chamber. The mean value of CMT indicates that membrane in...

Rice blast caused by Magnaporthe oryzae is one of the most devastating diseases of rice around the world and crop losses due to blast are considerably high. Many blast resistant rice varieties have been developed by classical plant breeding and adopted by farmers in various rice-growing countries. However, the variability in the pathogenicity of th...

Developing fragrant rice through marker-assisted/aided selection (MAS) is an economical and profitable approach worldwide for the enrichment of an elite genetic background with a pleasant aroma. The PCR-based DNA markers that distinguish the alleles of major fragrance genes in rice have been synthesized to develop rice scent biofortification throug...

The BC2F1 population was derived from a cross between rice variety, MR219 (susceptible to blast) and Pongsu Seribu 1 (resistant to blast). The objectives of this research were to know the inheritance pattern of blast resistance and to identify the linked markers associated with blast resistance in BC2F1 population. Sixteen microsatellite markers we...