Lasse Kvich

Lasse Kvich
University of Copenhagen · Faculty of Health and Medical Sciences Department of Immunology and Microbiology & Surgical Department at Zealand University Hospital

PhD student MSc of Health Science

About

13
Publications
1,824
Reads
How we measure 'reads'
A 'read' is counted each time someone views a publication summary (such as the title, abstract, and list of authors), clicks on a figure, or views or downloads the full-text. Learn more
148
Citations
Introduction
I have a great interest in clinical microbiology and the role of bacterial biofilms in chronic infections. In particular, the physiology of bacteria and how this influences later growth has been a focus area. I recently started a Ph.D. where I will combine these interests with a new specialty, colorectal cancer, where the relationship between specific bacteria and the presence of biofilm will be investigated concerning colorectal cancer carcinogenesis.
Additional affiliations
December 2015 - December 2019
University of Copenhagen
Position
  • Research Assistant

Publications

Publications (13)
Article
Full-text available
Objective: The aim of this study was to prospectively investigate the incidence of Propionibacterium acnes in thioglycollate broths reported as culture-negative at the Department of Clinical Microbiology, Rigshospitalet, to evaluate whether five days of incubation was enough to find all relevant cases. Method: Five hundred thioglycollate broths...
Article
Full-text available
Induction of a non-culturable state can have major consequences in a clinical perspective. Here, we show how restriction of O2 generates difficult-to-culture (DTC) bacteria during biofilm growth. A subpopulation of P. aeruginosa, S. aureus and S. epidermidis entered a DTC state after restriction of O2. These results demonstrate that habituation to...
Article
Full-text available
The microtiter assay is one of the most widely used methods for assessing biofilm formation. Though it has high throughput, this assay is known for its substantial deviation from experiment to experiment, and even from well to well. Since the assay constitutes one of the pillars of biofilm research, it was decided to examine the wells of a microtit...
Article
Pseudomonas aeruginosa is generally described as ubiquitous in natural settings, such as soil and water. However, because anecdotal observations and published reports have questioned whether or not this description is true, we undertook a rigorous study using three methods to investigate the occurrence of P. aeruginosa: we investigated environmenta...
Article
Full-text available
Chronic infections present a serious economic burden to health-care systems. The severity and prevalence of chronic infections are continuously increasing due to an aging population and an elevated number of lifestyle related diseases such as diabetes. Treatment of chronic infections has proven difficult, mainly due to the presence of biofilms that...
Article
Full-text available
The use of bone implants and prostheses has contributed to a revolution in modern medicine; however, in the beginning, not much was asked from the implant and prosthetic materials per se. Therefore, the next game-changer in orthopedic research will come from new material designs which for instance can aid in prevention of implant-associated bone in...
Article
Full-text available
Current evidence suggests that bacteria contribute to the development of certain cancers, such as colorectal cancer (CRC), partly by stimulating chronic inflammation. However, little is known about the bacterial impact on molecular pathways in CRC. Recent studies have demonstrated how specific bacteria can influence the major CRC-related pathways,...
Poster
Full-text available
This poster was presented at the Danish Surgical Society Annual Meeting (DKS) and displayed the methodological approach to visual characterization of bacteria in colorectal cancer (CRC) carcinogenesis, with particular attention to F. nucleatum and B. fragilis. For this purpose, we developed species-specific, synthetic DNA probes targeting these bac...
Article
Full-text available
The carcinogenic effects of microorganisms have been discovered in multiple cancer types. In urology, the development of squamous cell carcinoma of the bladder due to the parasitic infection with Schistosoma Mansoni is widely accepted. The oncogenic potential of biofilms has been studied in colorectal cancer and experimental studies have shown that...
Article
Full-text available
Coating surfaces with a copper-silver alloy in clinical settings can be an alternative or complementary antibacterial strategy to other existing technologies and disinfection interventions. A newly developed copper-silver alloy coating has a high antibacterial efficacy against common pathogenic bacteria in laboratory setups, and the purpose of this...
Preprint
Full-text available
Induction of a non-culturable state has been demonstrated for many bacteria. In a clinical perspective, the lack of growth due to these non-culturable bacteria can have major consequences for the diagnosis and treatment of patients. Here we show how anoxic conditioning (restriction of molecular oxygen, O 2 ) generates difficult-to-culture (DTC) bac...
Article
Full-text available
The protozoan parasite Toxoplasma gondii causes severe opportunistic infections. Here, we report an unexpected diagnosis of cerebral toxoplasmosis. T. gondii was diagnosed by 16S and D2LSU rDNA sequencing of a cerebral biopsy specimen and confirmed by T. gondii-specific PCR and immunohistochemistry. The patient was later diagnosed with HIV/AIDS. Co...
Poster
Full-text available
Identification of microorganisms usually takes 1-2 days. Using PCR and 16S rDNA sequencing, identification can be obtained the same day and patients can get a specific treatment faster. 16S is a small part of the ribosomal genome in bacteria, which is a conserved part with minimal changes through evolution, allowing differentiation between species...

Network

Cited By

Projects

Projects (4)
Project
The aim of this PhD project is to investigate the bacterial role in cancer carcinogenesis with special attention to colorectal cancer. Characterization of the bacteria i.e. what they are doing, how they respond to treatments and how they are interplaying with human cells will improve our understanding and lead to new strategies to treat and predict development of cancer. Colorectal cancer (CRC) is a genetic disease resulting from mutations, which follow well-established molecular pathways. Recently, several studies have linked the gut microbiota to CRC carcinogenesis. Microbial imbalance (dysbiosis) and known bacterial drivers play a significant role in inducing genomic mutations in human cells and/or exacerbate tumor-promoting inflammation.
Archived project
The aim of this project was to investigate the properties of oxygen restriction in biofilms in relation to later growth
Project
This project will investigate the heterogeneity of O2 and pH landscapes in and between biofilm aggregates to elucidate interactions between the chemical microenvironment and growth rates of pathogenic bacteria. The project will also unravel if interactions between bacterial strains influence the chemical microenvironment and how such community synergies affect growth characteristics of pathogens.