Lars Fieseler

Lars Fieseler
Zurich University of Applied Sciences | ZHAW · Institute of Food and Beverage Innovation (ILGI)

PhD

About

181
Publications
7,362
Reads
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2,067
Citations
Additional affiliations
September 2011 - present
Zurich University of Applied Sciences
Position
  • Head of Department
September 2011 - present
Zurich University of Applied Sciences
Position
  • Lecturer
April 2006 - August 2011
Institute of Food, Nutrition, and Health, ETH Zurich, Zurich, Switzerland
Position
  • PostDoc Position
Education
February 2001 - April 2005
University of Wuerzburg
Field of study
  • PhD student

Publications

Publications (181)
Article
Full-text available
Broad application of antibiotics gave rise to increasing numbers of antibiotic resistant bacteria. Therefore, effective alternatives are currently investigated. Bacteriophages, natural predators of bacteria, could work as such an alternative. Although phages can be highly effective at eliminating specific bacteria, phage resistance can be observed...
Article
Bacteriophages are highly selective in targeting bacteria. This selectivity relies on the specific adsorption of phages to the host cell surface. In this study, a Tn5 transposon mutant library of Erwinia amylovora, the causative agent of fire blight, was screened to identify bacterial receptors required for infection by the podovirus S6. Phage S6 w...
Article
Shiga toxin producing Escherichia coli (STEC) are common etiological agents of food borne illnesses and outbreaks, most often caused by consuming contaminated beef products, followed by raw vegetables and dairy products. Patients infected with E. coli O157 are more likely hospitalized than patients infected with non-O157 STEC, making E. coli O157 a...
Article
Background The NEMIS Technologies N-LightTM L. monocytogenes assay utilizes chemiluminescence designed for the qualitative detection of Listeria monocytogenes from environmental surface samples. Objective To validate the NEMIS Technologies N-LightTM L. monocytogenes assay as part of the AOAC Performance Tested MethodSM Program for the detection of...
Article
Full-text available
Methicillin-resistant Staphylococcus aureus (MRSA) can cause a wide range of infections from mild to life-threatening conditions. Its enhanced antibiotic resistance often leads to therapeutic failures and therefore alternative eradication methods must be considered. Potential candidates to control MRSA infections are bacteriophages and their lytic...
Article
Drone brood of Apis mellifera is often removed from the beehive to control the honeybee parasite Varroa destructor. Instead of discarding the drone brood, it could rather be used as a new food source for human nutrition. However, studies on microbiological hazards caused by edible insects are rare, especially in the case of drone brood. In this sur...
Article
In order to prevent microbial contamination of food, monitoring of the production environment, together with the rapid detection of foodborne pathogens have proven to be of utmost importance for Food Safety. Environmental monitoring should detect harmful pathogens at the earliest point in time in order for the necessary interventions to be taken. H...
Article
Full-text available
Bacteriophages of the Podoviridae family often exhibit so-called depolymerases as structural components of the virion. These enzymes appear as tail spike proteins (TSPs). After specific binding to capsular polysaccharides (CPS), exopolysaccharides (EPS) or lipopolysaccharide (LPS) of the host bacteria, polysaccharide-repeating units are specificall...
Article
Detection of Salmonella and L. monocytogenes in food samples by current diagnostic methods requires relatively long time to results (2-6 days). Furthermore, the ability to perform environmental monitoring at the factory site for these pathogens is limited due to the need for laboratory facilities. Herein, we report new chemiluminescence probes for...
Article
Detection of Salmonella and L. monocytogenes in food samples by current diagnostic methods requires relatively long time to results (2–6 days). Furthermore, the ability to perform environmental monitoring at the factory site for these pathogens is limited due to the need for laboratory facilities. Herein, we report new chemiluminescence probes for...
Article
Full-text available
Phages vB_EcoM-EP75 (EP75) and vB_EcoP-EP335 (EP335) specifically infect Shiga toxin (Stx)-producing Escherichia coli (STEC) O157 strains. EP75 has a genome size of 158,143 bp and belongs to the genus Vi1virus . The genome size of EP335 is 76,622 bp, and it belongs to the genus Phieco32virus .
Article
Full-text available
Bacteriophages represent a promising alternative for controlling pathogenic bacteria. They are ubiquitous in the environment, and their isolation is usually simple and fast. However, not every phage is suitable for biocontrol applications. It must be virulent (i.e., strictly lytic), non-transducing, and safe. We have developed a method for identify...
Article
Full-text available
Phages vB_EamP-S2 (S2) and vB_EamM-Bue1 (Bue1) infect the plant pathogen Erwinia amylovora . S2 has a genome size of 45,495 bp and belongs to the genus SP6virus . The genome size of Bue1, related to Salmonella phage Vil, is 164,037 bp. Both phages possess a depolymerase enzyme, a frequent feature of E. amylovora phages.
Article
Full-text available
To date, a small number of jumbo myoviruses have been reported to possess atypical whisker-like structures along the surface of their contractile tails. Erwinia amylovora phage vB_EamM_Y3 is another example. It possesses a genome of 261,365 kbp with 333 predicted ORFs. Using a combination of BLASTP, Interproscan and HHpred, about 21% of its putativ...
Article
Full-text available
Wall teichoic acids (WTAs) are the most abundant glycopolymers found on the cell wall of many Gram-positive bacteria, whose diverse surface structures play key roles in multiple biological processes. Despite recent technological advances in glycan analysis, structural elucidation of WTAs remains challenging due to their complex nature. Here, we emp...
Article
To enable specific and tightly controlled gene expression both in-vitro and during the intracellular lifecycle of the pathogen Listeria monocytogenes, a TetR-dependent genetic induction system was developed. Highest concentration of cytoplasmic TetR and best repression of tetO-controlled genes was obtained by tetR expression from the synthetic prom...
Article
Full-text available
Erwinia amylovora is the causative agent of fire blight, a devastating plant disease affecting members of the Rosaceae. Alternatives to antibiotics for control of fire blight symptoms and outbreaks are highly desirable, due to increasing drug resistance and tight regulatory restrictions. Moreover, the available diagnostic methods either lack sensit...
Article
The microbial quality of drinking and environmental water is usually determined by culture-based detection of fecal indicator bacteria according to ISO reference methods 16649-1 and 7899-2, respectively. Because of an increasing demand for rapid, culture-independent methods,we tested three quantitative polymerase chain reaction (qPCR) approaches fo...
Article
Full-text available
Bacteriophages have regained much attention as biocontrol agents against bacterial pathogens. However, with respect to stability, phages are biomolecules and are therefore sensitive to a number of environmental influences. UV-irradiation can readily inactivate phage infectivity, which impedes their potential application in the plant phyllosphere. T...
Article
Full-text available
To monitor dominant species of lactic acid bacteria during cocoa bean fermentation, i.e. Lactobacillus plantarum and L. fermentum, a fast and reliable culture-independent qPCR assay was developed. A modified DNA isolation procedure using a commercial kit followed by two species-specific qPCR assays resulted in 100% sensitivity for L. plantarum and...
Conference Paper
Full-text available
Facility management (FM) is present in healthcare. According to the International Facility Management Association (IFMA) guidelines, an organisation is obliged to take precautions to enable continuous business operation and reduce hazards. As FM organizations or facilities services are sometimes responsible for water systems, and hence in the reduc...
Article
Listeria monocytogenes can grow as a saphrophyte in diverse habitats, e.g., soil, rivers, lakes, and on decaying plant material. In these environments, the bacteria are frequently exposed to predatory protozoa such as Acanthamoeba. Although L. monocytogenes is a facultative intracellular pathogen it does not infect or survive intracellular in Acant...
Chapter
Today, in terms of food safety and biocontrol, sales professionals promote phages as 'natural born killers'. This is mainly because bacteriophages feature unique and efficient mechanisms to specifically target and eliminate bacteria. According to the International Committee on Taxonomy of Viruses, tailed phages are further separated into three dist...
Article
The depolymerase enzyme (DpoL1) encoded by the T7-like phage L1 efficiently degrades amylovoran, an important virulence factor and major component of the extracellular polysaccharide (EPS) of its host, the plant pathogen Erwinia amylovora. Mass spectrometry analysis of hydrolysed EPS revealed that DpoL1 cleaves the galactose-containing backbone of...
Article
Full-text available
Acid production from rhamnose is a characteristic phenotype of Listeria monocytogenes. We report the identification of the rhamnose transport and utilization operon located at lmo2846 to lmo2851, including the rhamnose-dependent promoter P(rha). Expression of reporter genes under control of P(rha) on a single copy integration vector demonstrated it...
Article
Despite its prominent role as an intracellular human pathogen, Listeria monocytogenes normally features a saprophytic lifestyle, and shares many environmental habitats with predatory protozoa. Earlier studies claimed that Acanthamoeba may act as environmental reservoirs for L. monocytogenes, whereas others failed to confirm this hypothesis. Our fin...
Article
Many marine sponges (Porifera) are known to contain large amounts of phylogenetically diverse microorganisms. Sponges are also known for their large arsenal of natural products, many of which are halogenated. In this study, 36 different FADH(2)-dependent halogenase gene fragments were amplified from various Caribbean and Mediterranean sponges using...
Article
Full-text available
A diverse set of 24 novel phages infecting the fire blight pathogen Erwinia amylovora was isolated from fruit production environments in Switzerland. Based on initial screening, four phages (L1, M7, S6, and Y2) with broad host ranges were selected for detailed characterization and genome sequencing. Phage L1 is a member of the Podoviridae, with a 3...
Article
Bacteriophages and phage encoded endolysins exhibit valuable properties to specifically target and control unwanted bacteria in foods. Here, the construction of reporterphages for pathogen detection, characteristics of phage encoded endolysins, and application of phages for biocontrol purposes are summarized. Moreover, the standards required for us...
Article
Full-text available
Poribacteria were found in nine sponge species belonging to six orders of Porifera from three oceans. Phylogenetic analysis revealed four distinct poribacterial clades, which contained organisms obtained from several different geographic regions, indicating that the distribution of poribacteria is cosmopolitan. Members of divergent poribacterial cl...